• Title/Summary/Keyword: Slow and rapid methods

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Analysis of Attached Bacterial Community of Biological Activated Carbon Process Using FISH (FISH 기법을 이용한 생물활성탄 공정에서의 운전기간별 부착 박테리아 군집변화 분석)

  • Son, Hyeng-Sik;Son, Hee-Jong;Park, Geun-Tae;Lee, Sang-Joon
    • Journal of Environmental Science International
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    • v.22 no.1
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    • pp.25-35
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    • 2013
  • The concentration of organic compounds was analyzed at each step of BAC process though $BDOC_{total/rapid/slow}$. Further, bacteria communities and biomass concentrations measured FISH and ATP methods were analyzed. The bed volume (BV) of steady state is different from that of based on assessment of organic compounds removal. Bed volumes in DOC, $BDOC_{rapid}$ and $BDOC_{total/slow}$ removal at steady state were around 27,500 (185.8 day), 15,000 (101.4 day) and 32,000 (216.2 day), respectively. A biomass didn't change after the bed volume reached 22,500 (152.0 day) according to analyzing ATP concentration of bacteria. The concentration of ATP was 2.14 ${\mu}g/g$ in BV 22,500 (152.0 day). The total bacterial number was $4.01{\pm}0.4{\times}10^7$ cells/g at the bed volume 1,150 (7.8 day) (the initial operation) and the number of bacteria was $9.27{\pm}0.2{\times}10^9$ at the bed volume 58,560 395.7 day) that increased more than 200 times. Bacterial uptrend was reduced and bacterial communities were stabilized since BV 18,720 (126.5 day). When BV were 1,150 (7.8 day), 8,916 (60.2 day), 18,720 (126.5 day), 31,005 (209.5 day), 49,632 (335.3 day), 58,560 (395.7 day), a proportion of total bacteria for the Eubacteria were 60.1%, 66.0%, 78.4%, 82.0%, 81.3% respectively. ${\gamma}$-Proteobacteria group was the most population throughout the entire range. The correlation coefficient ($r^2$) between Eubacteria biomass and ATP concentration was 0.9448.

The Relationship between Pulse Pattern and NIHSS Score in Early Stroke Patient;Multi Center Trials (초기 중풍 환자에 있어서 맥상(脈象)과 NIHSS과의 상관성 연구;다기관 임상연구)

  • Ma, Mi-Jin;Gang, A-Mi;Choi, Dong-Jun;Han, Chang-Ho;Lee, Won-Chul;Jun, Chan-Yong;Cho, Ki-Ho;Choi, Sun-Mi
    • The Journal of Internal Korean Medicine
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    • v.28 no.4
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    • pp.727-732
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    • 2007
  • Objectives : This study investigated using National Institutes of Health Strokes Scale in acute and subacute stroke patients and evaluated relativity of pulse pattern to NIHSS. Methods : 104 acute and subacute stroke patients were selected from 4 oriental medical hospitals from April 2007 to May 2007. The patients were admitted to hospital within 1 month after stroke. Pulse feeling diagnosis was done by oriental medical doctor and classified into 7 subtypes. Results : There were significant results statistically between slow/rapid pulse and NIHSS score in the male group; in the female group, there were no significant results statistically but the results showed that slow pulse has lower NIHSS score than rapid pulse. Patients with surging pulse had lower NIHSS score than the other patients. Another pulse pattern had no relativity to NIHSS score.

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Cryopreservation of Oocytes and Embryos by Vitrification (유리화 방법에 의한 난자와 수정란의 동결보존)

  • Gupta, Mukesh Kumar;Lee, Hoon-Taek
    • Clinical and Experimental Reproductive Medicine
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    • v.37 no.4
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    • pp.267-291
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    • 2010
  • Life can be kept in suspended animation either before fertilization at oocyte stage or after fertilization at different stages of embryonic development for a variety of reasons. It not only has potential applications in fertility preservation and management in human but also has important roles in the preservation and management of animal genetic resources, low-cost international movement of selected genetics, and rapid dissemination of germplasm through assisted reproductive technologies (ART) and genetic engineering. Currently, slow-freezing and vitrification are the two approaches by which oocytes and embryos can be cryopreserved for long-term storage. Both of these methods have their own advantages and disadvantages but allow the cryopreservation of oocytes and embryos with comparable efficiency. Vitrification of oocyte and embryos, although proven successful just 13 years after slow-freezing, is generally considered an emerging technology and appears to slow gain acceptance in both animal and human ART despite having controversial storage and contamination issues. In this manuscript, we discuss the basic techniques of oocyt/embryo cryopreservation and review the current status and recent developments in vitrification.

Hydrological Consequences of Converting Forestland to Coffee Plantations and Other Agriculture Crops on Sumber Jaya Watershed, West Lampung, Indonesia

  • Manik, Tumiar Katarina;Sidle, Roy Carl
    • Journal of Forest and Environmental Science
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    • v.34 no.4
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    • pp.293-303
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    • 2018
  • Sumber Jaya (54,194 hectares) is a district in West Lampung, Indonesia, located at the upper part of Tulang Bawang watershed. This watershed is one major water resource for Lampung Province, but has become a focal point of discussion because of the widespread conversion of forestland to coffee plantations and human settlements which lead to environmental and hydrological problems. This research aimed to evaluate Sumber Jaya watershed affecting by rapid land use change using hydrological methods as a base for watershed management. Nested catchment structure consisted of eight sub-catchments was employed in this research to assess scaling issues of land use change impacts on rainfall-runoff connections. Six tipping bucket rain gages were installed on the hill slopes of each sub-catchment and Parshall flumes were installed at the outlets of each sub-catchment to monitor stream flow. First, unit hydrograph that expressed the relationship of rainfall and runoff was computed using IHACRES model. Second, unit hydrograph was also constructed from observations of input and response during several significant storms with approximately equal duration. The result showed that most of the storm flow from these catchments consisted of slow flow. A maximum of about 50% of the effective rainfall became quick flow, and only less than 10% of remaining effective rainfall which was routed as slow flow contributed to hydrograph peaks; the rest was stored. Also, comparing peak responses and recession rates on the hydrograph, storm flow discharge was generally increased slowly on the rising limb and decreased rapidly on the falling limb. These responses indicated the soils in these catchments were still able to hold and store rain water.

Studies on cryopreservation of nuclear transplanted mouse embryos (마우스 핵이식란의 동결에 관한 연구)

  • Lee, Byeong-chun;Jo, Choong-ho;Hwang, Woo-suk
    • Korean Journal of Veterinary Research
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    • v.33 no.1
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    • pp.171-178
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    • 1993
  • The present study was carried out to investigate the developmental potency to blastocyst after freezing and thawing of nuclear transplanted 2-cell embryos. The nuclei from 2-, 4- and 8-cell mouse embryos were transferred into enucleated 2-cell embryos, and the reconstituted embryos were submitted to direct current(DC) pulse at output voltage of 2.0 kV/cm for $100{\mu}$ sec to induce cell fusion. The recovery rate and developmental potency to blastocyst after freezing and thawing of nuclear transplanted 2-cell embryos was investigated. 1. The recovery rate of nuclear transplanted 2-cell embryos in normal morphology after freezing and thawing was significantly higher in rapid freezing(DMSO 4.5M) than in slow cooling(p<0.01). 2. When the recovered embryos in normal morphology were cultured in vitro, there were no significant differences in the developmental potency to blastocyst between the freezing methods and the concentrations of cryoprotectant. In summary, these experiments have proved that rapid freezing method(DMSO 4.5M) is effective in nuclear transplanted 2-cell mouse embryos. If improved micromanipulation techniques and freezing are combined, nuclear transplantation technique will contribute to the improvement of productivity in livestock animals.

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A Case Report of Idiopathic Precocious Puberty in Two Children (특발성 성조숙증으로 진단받은 환아 2례에 대한 증례보고)

  • Lee, Hye Lim;Yoo, Hwa Seung;Park, Seung Chan
    • The Journal of Pediatrics of Korean Medicine
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    • v.31 no.1
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    • pp.74-81
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    • 2017
  • Objectives The purpose of this study is to report the case of precocious puberty in two children treated by taking herbal medicine. Methods Two patients diagnosed with precocious puberty were prescribed Jogyeongseongjang-tang and were observed the effect of treatment on height, body weight, body composition, sex hormone test. Results During the treatment period, rapid progression of puberty was inhibited, and slow changes in sex hormones and steady growth were achieved. Conclusions This study showed the long-term effects of herbal medicine in treating precocious puberty, but further studies should be conducted for scientific validation.

Studies on the Development of Easy Cryopreservation Technique of Bovine Embryos I. Effects of Kinds, Concentration and Freezing Method of Cryoprotectants on In Vitro Developmental Rates of Embryos (소 수정란의 간이 동결기법 개발에 관한 연구 I. 내동제의 종류, 농도 및 동결방법이 체외발생율에 미치는 영향)

  • 김상근;남윤이;현병화;석호봉
    • Korean Journal of Animal Reproduction
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    • v.21 no.2
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    • pp.95-102
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    • 1997
  • The studies on the carried out to investigate the effective concentration of cryoprotectant agents and sucrose by one-step straw method of bovine embryos. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/ml PMSG(Sigma, USA), 10 IU/ml hCG(Sigma, USA), 1$\mu\textrm{g}$/ml $\beta$-estradiol(Sigma, USA) and 10% FCS for 24~48 hrs in incubator with 5% CO2 in air at 38.5$^{\circ}C$ and then matured oocytes were again cultured for 12~18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 3$0^{\circ}C$ water. Survival and in vitro developmental rate was defined as devellpmental rate on in vitro culture or FDA-test. The results are smmarized as followes : 1. The high in vitro developmental rates of bovine frozen embryos after rapidly thawed in freezing medium was attained 2.0M glycerol, 2.0M DMSO, 1M or 2.0M propanediol. 2. The high in vitro developmental rates of bovine frozen embryos after rapidly thawed in freezing medium was obtained single cryoprotectant(6.7~17.4%) than mixed cryoprotectants(6.7~16.7%). 3. In vitro developmental rate of bovine embryos after rapid frozen-thawing in the freezing medium added 0.25M and 0.50M sucrose were higher cleavage rate than those of sucrose concentration of 0.75M and 1.00M. 4. The freezing methods on in vitro developemental rates of bovine embryos was attained slow freezing method(9.70~15.6%) higher than rapid freezing method(9.4~13.3%).

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Changes of Nitrogeneous Compounds Depending upon the Curing Methods in Burley Tobacco (버어리종 건조방법에 따른 질소화합물의 변화)

  • 백순옥;한상빈;배성국
    • Journal of the Korean Society of Tobacco Science
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    • v.25 no.1
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    • pp.27-33
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    • 2003
  • This study was conducted to determine the changes of nitrogen compounds in lamina and midrib during the curing process with the different curing methods, such as priming and stalk-cut curing. After KB 108 burley tobacco was cultivated by the different fetilization levels such as standard and 20% higher, only the tips and leaf were harvested. Though the contents of total alkaloid and total nitrogen were similar in lamina, midrib showed a very low contents of those components by the different curing method and fertilization levels. The content of nitrate-nitrogen in lamina increased during a middle of curing process, and then these compound was decreased during an end of curing process by stalk-cut curing method. Nitrate-nitrogen contents in the lamina by the priming curing showed a high level caused by rapid drying process during an end of curing process. That component in midrib was 5-6 times higher than that of lamina. The contents of ammonia-nitrogen in the lamina and midrib were increased during a curing process. Though those amount in tips showed a similar by a different fetilization and curing method, midrib of stalk-cut curing showed a slow increasing during a curing process.

Deterministic Function Variable Step Size LMS Algorithm (결정함수 가변스텝 LMS 알고리즘)

  • Woo, Hong-Chae
    • Journal of the Institute of Convergence Signal Processing
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    • v.12 no.2
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    • pp.128-132
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    • 2011
  • Least mean square adaptive algorithms have played important role in radar, sonar, speech processing, and mobile communication. In mobile communication area, the convergence rate of a LMS algorithm is quite important. However, LMS algorithms have slow and non-uniform convergence rate problem For overcoming these shortcomings, various variable step LMS adaptive algorithms have been studied in recent years. Most of these recent LMS algorithms have used complex variable step methods to get a rapid convergence. But complex variable step methods need a high computational complexity. Therefore, the main merits such as the simplicity and the robustness in a LMS algorithm can be eroded. The proposed deterministic variable step LMS algorithm is based upon a simple deterministic function for the step update so that the simplicity of the proposed algorithm is obtained and the fast convergence is still maintainable.

Evidence of Significant Effects of Stunning and Chilling Methods on PSE Incidences

  • Park, B.Y.;Kim, J.H.;Cho, S.H.;Hah, K.H.;Lee, S.H.;Choi, C.H.;Kim, D.H.;Lee, J.M.;Kim, Y.K.;Ahn, J.N.;Hwang, I.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.2
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    • pp.257-262
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    • 2007
  • The current study was conducted to investigate the optimum stunning voltage and chilling regime with emphasis on reduction in pale, soft and exudative (PSE) pork. The experiments were conducted at seven Korean major pig abattoirs using a total of 91,082 industrial population. Frequencies of PSE meat was found to be significantly (p<0.05) increased as stunning voltage was elevated from 220-240 (13.14%), 250-280 (29.32%) to 430 volts (36.74%). Chilling methods after slaughter, either with cold water showing or rapid chilling reduced PSE meat by 22% compared to a classic chiller-based slow chilling regime. The current study also revealed that chiller temperature during the first 90 minutes had a significant (p<0.001) effect on PSE incidences. Pigs chilled between -5 to $7^{\circ}C$ resulted in the lowest PSE meat (17.8%), followed by higher than $7^{\circ}C$ (21.3%) and lower than $-5^{\circ}C$ (37.5%). The current data implies that low voltage stunning method (eg., 220-240 volts), followed by rapid chilling regime, maintaining chiller temperature between approximately -5 to $7^{\circ}C$ could reduce PSE incidences.