• 한국의류산업학회지
• /
• 제12권4호
• /
• pp.531-537
• /
• 2010

The purpose of this study was to review physiological responses and subjective sensations in the cold environment when the subjects wore ensemble with different clo values. Seven healthy male subjects participated in this experiment. This experiment was conducted in a climatic chamber with $-10^sC$ and 50%RH. Subjects wore five different kinds of ensemble[C1 (4.453 clo), C2 (3.452 clo), C3 (2.865 clo), C4 (2.387 clo), and C5 (2.280 clo)]. The experiment was composed of 20 min of rest period, 20min of treadmill exercise(6 km/h) period, 30 min of recovery period. We monitored skin temperature on 7 sites, clothing microclimate and subjective sensations. The clo value had positive correlations with mean skin temperature and clothing microclimate. The subjects feel more warm and humid as the clo value goes up. The subjects reported comfort when they wore C1 and C2 ensemble having over 3 clo value. However, they felt less comfortable during the exercise period since there was high humidity. Skin temperature on the extremities were more dramatically changed by the exercise rather than clo value. Thus it seems that in the cold environment, heat balance can mostly be controlled by the choice of clothing, and the clothes with high clo values can provide higher insulation. In conclusion, our findings suggest that it would be more effective to control clo value depending on the activity level for maintaining comfort level in the cold environment.

• 한국동물생명공학회지
• /
• 제34권1호
• /
• pp.20-29
• /
• 2019

Soy isoflavones have been reported to possess many physiological activities such as antioxidant activity and inhibition of cancer cell proliferation. This study investigated the photoprotective effects of soybean extract in human fibroblast cell line and hairless mice model. Human fibroblast was treated with soybean extract before and after ultraviolet B (UVB; 290-302 nm) irradiation. In the soybean extract treated group, the cells showed better resistance to ultraviolet (UV) than control group. The amount of type I collagen recovered from the soybean treated group was higher than the vehicle group exposed to UV-induced damage. Moreover, increased expression of metalloproteinases-1 as a result of UV irradiation was suppressed by the soybean extract. Female mice were orally administered soybean extract and irradiated with UVB light for 8 weeks. The effects of the soybean extract on the skin appearance, collagen deposition and epidermal thickness in the UV-damaged mouse skin were analyzed using histopathological methods. In soybean extract treated group, the skin had a better morphology than that of the control group. Furthermore, the amount of type I collagen was increased and overexpression of MMP-1 was reduced in the soybean extract group compared to vehicle group. Additionally, up-regulation of pro-inflammatory cytokines induced by UV irradiation was suppressed by dietary soybean extract treatment. It appears that soybean extract had a photoprotective effect, including anti-aging and anti-inflammatory effect, from UV-induced damage in not only human fibroblast, but also hairless mice. We confirmed that these effects were possibly due to promotion of collagen synthesis and inhibition of MMP-1 expression.

• 한국의류산업학회지
• /
• 제10권5호
• /
• pp.668-675
• /
• 2008

The purpose of this study was to collect data of skin temperature and physiological responses which is useful for standardization of insulation measurement in various garments. And we investigated sex and season difference of physiological responses of 60's males and 60's females in hot and cold environment. Healthy ten 60's males and ten 60's females volunteered as subjects. The experiment was conducted three times; One for winter condition($5^{\circ}C$, 45%), another for summer condition($30^{\circ}C$, 65%) and the other for nude condition as control($30^{\circ}C$, 65%). The subjects were sitting for 1 hour with suitable ensemble on each experimental condition in climate chamber. We measured skin temperature, rectal temperature, heart rate, oxygen uptake, sweat rate, blood flow, blood pressure and subjective sensations. We found that skin temperature and most of physiological responses were higher in male subject, summer condition than in female subject, winter condition.

• 대한화장품학회지
• /
• 제31권3호
• /
• pp.253-257
• /
• 2005

피부노화의 정도를 판정하기 위해 사용되는 주름측정법들은 객관성과 재현성의 확보가 중요한 요소이다. 최근의 경향은 주름의 형태나 깊이에 주는 영향을 최소화하기 위해 주름 측정시 피부에 직접 기계나 도구를 접촉하지 않고 측정하는 비접촉식 측정방법으로 빠르게 전환되고 있는 상황이다. 저자들은 주름측정 기술의 변천 과정을 간단히 살펴보고, 비접촉식 fringe projection 방식의 미세주름 측정기기인 PRIMOS를 중심으로 측정원리, 특징들을 접촉식 측정방법인 모사판을 이용한 방법과 비교하였다.

• Journal of Microbiology and Biotechnology
• /
• 제33권1호
• /
• pp.135-141
• /
• 2023

Protection of skin cells from chronic infrared-A (IRA) irradiation is crucial for anti-photoaging of the skin. In this study, we investigated the protective activity of Rg3(S) and Rg3(S)-incorporated anionic soybean lecithin liposomes (Rg3/Lipo) with a size of approximately 150 nm against IRA-induced photodamage in human fibroblasts. The formulated Rg3/Lipo showed increased solubility in aqueous solution up to a concentration of 200 ㎍/ml, compared to free Rg3(S). In addition, Rg3/Lipo exhibited superior colloidal stability in aqueous solutions and biocompatibility for normal human dermal fibroblasts (NHDFs). After repeated IRA irradiation on NHDFs, elevated levels of cellular and mitochondrial reactive oxygen species (ROS) were greatly reduced by Rg3(S) and Rg3/Lipo. In addition, cells treated with Rg3/Lipo exhibited noticeably reduced apoptotic signals following IRA irradiation compared to untreated cells. Thus, considering aqueous solubility and cellular responses, Rg3/Lipo could serve as a promising infrared protector for healthy aging of skin cells.

• 생명과학회지
• /
• 제26권8호
• /
• pp.983-989
• /
• 2016

체내를 구성하는 모든 세포는 시간이 흐름에 따라 그들의 주위환경에 좌우되어 분화, 괴사, 세포자살, 세포노화와 같은 운명을 경험한다. 이러한 세포과정에서 발생하는 실수가 암, 염증, 노화 및 질병과 같은 표현형에서의 여러가지 이상을 발생시킨다. 천연물로부터 유래한 항 노화 화합물을 탐색하기 위해서는 새로운 전략과 접근방식이 요구된다. 그러므로, 여기서는 핵심적인 역할을 하는 타켓 단백질에 대하여 설명한다. 먼저 기질금속단백질분해효소(MMPs)는 노화마커로 암전이, 만성염증 및 피부노화에 관여한다. 특히 히스톤 탈아세틸화효소(HDACs)는 모델동물의 수명을 연장시키려고 노력하는 노화연구원들에게 큰 관심의 대상이다. 뿐만 아니라, 여기서 p53, IGF-1 및 SIRT1이 중요한 역할을 하는 세포노화와 관련된 신호경로에 대하여 기술한다. 더욱이, 자가포식과정이 노화와 관련한 신호경로에도 관여하고 있다. 세포노화의 신호경로를 조절할 수 있는 여러가지 새로운 화합물도 본 총설논문에서 소개된다. 여기서 우리는 노화기전에 대한 분자기반 및 노화마커 개발에 대한 새로운 통찰력을 제공하려고 한다. 뿐만 아니라 소개되는 화합물은 노화와 관련 있는 질병의 예방 및 치료를 위하여 의학적으로 응용이 가능하다.

• Toxicological Research
• /
• 제28권2호
• /
• pp.113-116
• /
• 2012

Various kinds of positive effects of green tea extracts had been studied for long time which included anti-inflammation, anti-aging, and cardiometabolic effects. Although topical steroid and non-steroidal calcineurin inhibitors may control clinical symptoms of allergic contact dermatitis, some of patients also present allergic reaction to these topical agents. Therefore, we have tried green tea extracts for managing this skin disorder with expectation of anti-inflammatory effect without potential side effects including skin irritation and toxic responses. The toxicity test of green tea extract also did not show any sign of irritation in the skin throughout the test period. Moderate severity of allergic contact dermatitis presented satisfactory clinical outcome at second week follow-up which was final visit of outpatient. This result mean that green tea extract has a positive effect for managing allergic contact dermatitis but its potency and efficacy seem to be so not strong enough to control moderate severity allergy skin lesion. In this pilot study, we were able to conclude that green tea cell extracts might be applied for potential anti-inflammatory soaking without skin toxicity.

• Environmental Analysis Health and Toxicology
• /
• 제24권2호
• /
• pp.159-167
• /
• 2009

To investigate the alleviative effects of Peonia japonica water extract(PJWE) on inflammation and skin barrier damage, both the irradiation of UVB and the application of squalene monohydroperoxide (Sq-OOH) to the backs of hairless mice were performed for 4 weeks. And at the same time experimental materials were applied topically. The skin erythema indices for the positive control (PC, 0.01% retinoic acid) and experimental (E, PJWE) groups were lower than that of the control (C) group. Whereas both the lipid and water capacities for the PC and E groups were higher than those of the C group. Epidermis and dermis of the C group were remarkably thickened in comparison with the PC and E groups. Relatively much less number of inflammatory cells, including lymphocytes, neutrophils and macrophages were found in dermis of the PC and E groups compared with the C group. Lipid lamellae of the C group were broken severely showing an irregular arrangement and lipid content was much reduced. Whereas those of the PC and E groups were almost intact with a regular arrangement, which were similar to that of the N group. Taken the results all together, it was confirmed that PJWE could be effective natural herbal material for the alleviation of inflammation and skin barrier damage in hairless mice skin which were induced by UVB and Sq-OOH.

• Biomolecules & Therapeutics
• /
• 제22권4호
• /
• pp.321-327
• /
• 2014

Collagen pentapeptide (Lys-Thr-Thr-Lys-Ser, KTTKS) and its palmitoylated derivative (pal-KTTKS) have received a great deal of attention as cosmeceutical ingredients for their anti-wrinkle effects. The objective of this study was to evaluate stability and permeability of KTTKS and pal-KTTKS in hairless mouse skin. In this study, a liquid chromatography-tandem mass spectrometric method was developed for the quantification of pal-KTTKS, and used for stability and permeability studies. Stability studies were performed using skin extracts and homogenates. Both KTTKS and pal-KTTKS were rapidly degraded, but pal-KTTKS was more stable than KTTKS. When protease inhibitors were added, the stability of both compounds (KTTKS and pal-KTTKS) improved significantly. In the skin permeation study, neither KTTKS nor pal-KTTKS was detected in the receptor solution, which indicates that neither compound could permeate through the full-thickness hairless mouse skin in the experimental conditions of this study. While KTTKS was not detected in any of the skin layers (the stratum corneum, epidermis, and dermis), pal-KTTKS was observed in all skin layers: $4.2{\pm}0.7{\mu}g/cm^2$ in the stratum corneum, $2.8{\pm}0.5{\mu}g/cm^2$ in the epidermis, and $0.3{\pm}0.1{\mu}g/cm^2$ in the dermis. In conclusion, this study indicated that pal-KTTKS had greater stability and permeability than that of un-modified KTTKS, and may be a useful anti-wrinkle and anti-aging cosmeceutical agent.

• Archives of Plastic Surgery
• /
• 제42권3호
• /
• pp.282-287
• /
• 2015

Background Morphological changes that accompany aging, such as wrinkles and skin laxity, are particularly prominent on facial skin. Recently, facial rejuvenation using the hydrofilling effect of hyaluronic acid (HA) filler has been employed for improvement of skin texture. In this study, we studied rejuvenating effects of stabilized HA (Restylane Vital) through direct intradermal injections. Methods A total of 30 female patients underwent a series of procedures on face, including three sessions at intervals of four weeks. A total of 2 mL of Restylane Vital was injected along the whole face using an automatic injector. Improvement of skin surface roughness, elasticity, brightness, moisture, and fine wrinkles was evaluated. Patient satisfaction was evaluated, and pictures of patients were taken at each visit and 6 months after last treatment session. Scoring for each patient was performed by three doctors according in five subjects. Moisture, oil and elasticity were measured before the procedure and before the last treatment in 10 patients. Results The majority of patients (77%) were satisfied with the therapeutic outcomes. Approximately 66% of patients responded that the effects of this procedure persisted for longer than four months, and the majority of patients (77%) wanted to undergo this procedure again and would recommend this procedure to acquaintances. Regarding doctors' evaluation, scores for improvement of skin surface roughness, elasticity, and brightness were significantly higher than those for improvement of moisture and fine wrinkle. Conclusions Intradermal injection of HA can have a rejuvenating effect on dry and tired facial skin, especially in improvement of skin surface roughness.