• Journal of Nutrition and Health
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• 제39권4호
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• pp.323-330
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• 2006

Peripheral insulin resistance in obese/type II diabetes animals results from an impairment of insulin-stimulated glucose uptake into skeletal muscle. Insulin stimulate the translocation of GLUT4 from intracellular location to the plasma membrane. Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) is implicated in mediation of fusion of GLUT4-containing vesicle with the plasma membrane. Present study investigated regulatory effects of Rhodiola sachalinensis administration and exercise training on the expression of GLUT4 protein and SNAREs protein in skeletal muscles of obese Zucker rats. Experimental animals were randomly assigned into one of five groups ; lean control(LN), obese control(OB), exercise-treated(EXE), Rhodiola sachalinensis-treated(Rho), combine of Rho & EXE (Rho-EXE). All animals of exercise training (EXE, Rho-EXE) performed treadmill running for 8 weeks, and animals of Rho groups (Rho, Rho-EXE) were dosed daily by gastric gavage during the same period. After experiment, blood were taken for analyses of glucose, insulin, and lipids levels. Mitochondrial oxidative enzyme (citrate synthase, CS ; $\beta$-hydroxyacyl-CoA dehydrogenase, $\beta$-HAD) activity were analysed. Skeletal muscles were dissected out for analyses of proteins (GLUT4, VAMP2, syntaxin4, SNAP23). Results are as follows. Exercise and/or Rhodiola sachalinensis administration significantly reduced body weight and improved blood lipids (TG, FFA), and increased insulin sensitivity. Endurance exercise significantly increased the activity of mitochondrial enzymes and the expression of GLUT4 protein, however, administration of Rhodiola sachalinensis did not affect them. The effect of exercise and/or Rhodiola sachalinensis administration on the expression of SNARE proteins was unclear. Our study suggested that improvement insulin sensitivity by exercise and/or Rhodiola sachalinensis administration in obese Zucker rats is independent of expression of SNARE proteins.

• 대한치과교정학회지
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• 제31권4호
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• pp.415-424
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• 2001

교정치료에 있어서 anchorage는 진단 및 치료계획에서부터 치료 종료 단계까지 교정의사가 항상 염두에 두면서 치료를 진행 해야 하는 중요한 요소이다. 환자의 적극적인 협조를 필요로 하는 전통적인 anchorage 조절법 보다는 miniscrew 같은 skeletal anchorage 가 좀더 효과적인 방법으로 제시되어 지고 있어, miniscrew의 교정적 이용 시 고려사항에 대하여 전반적인 고찰과 증례보고를 통해서 niniscrew의 다양한 임상적 적용에 대해서 살펴보았다. 또한 midpalate부위가 miniscrew의 식립 부위로서 가지는 장점과 식립 시의 주의사항을 알아보았으며 skeletal anchorage 가 교정에 도입되면서 치료개념과 방법에 있어서의 변화를 요약하면 다음과 같다. 1. 절대적인 개념의 Anchorage가 도입 되었다. 2. 생역학적 면에서 치아의 치체이동이 쉬워지고 determinate system이 설계되어 질수 있다. 3. 기존의 수술로만 가능했던 치료들 중 일부는 교정 치료만으로 치료가 가능하게 되었다.

• The Journal of Korean Physical Therapy
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• 제20권1호
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• pp.57-65
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• 2008

Purpose: Heat shock proteins (HSPs) are a group of proteins that are activated when cells are exposed to a variety of environmental stresses, such as infection, inflammation, exposure to toxins, starvation, hypoxia, brain injury, or water deprivation. The activation of HSPs by environmental stress plays a key role in signal transduction, including cytoprotection, molecular chaperone, anti-apoptotic effect, and anti-aging effects. However, the precise mechanism for the action of small HSPs, such as HSP27 and mitogen-activated protein kinases (MAPKs: extracellular-regulated protein kinase 1/2 (ERK1/2), p38MAPK, stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK), is not completely understood, particularly in application of cell stimulators including platelet-derived growth factor (PDGF), angiotensin II (AngII), tumor necrosis factor $\alpha$ (TNF$\alpha$), and $H_2O_2$. This study examined the relationship between stimulators-induced enzymatic activity of HSP27 and MAPKs from rat smooth and skeletal muscles. Methods: 2-dimensional electrophoresis (2DE) and matrix assisted laser desorption ionizationtime-of-flight/time-of-flight (MALDI-TOF/TOF) analysis were used to identify HSP27 from the intact vascular smooth and skeletal muscles. Three isoforms of HSP27 were detected on silver-stained gels of the whole protein extracts from the rat aortic smooth and skeletal muscle strips. Results: The expression of PDGF, AngII, TNF$\alpha$, and $H_2O_2$-induced activation of HSP27, p38MAPK, ERK1/2, and SAPK/JNK was higher in the smooth muscle cells than the control. SB203580 (30${\mu}$M), a p38MAPK inhibitor, increased the level of HSP27 phosphorylation induced by stimulators in smooth muscle cells. Furthermore, the age-related and starvation-induced activation of HSP27 was higher in skeletal muscle cells (L6 myoblast cell lines) and muscle strips than the control. Conclusion: These results suggest, in part, that the activity of HSP27 and MAPKs affect stressors, such as PDGF, AngII, TNF$\alpha$, $H_2O_2$, and starvation in rat smooth and skeletal muscles. However, more systemic research will be needed into physical therapy, including thermotherapy, electrotherapy, radiotherapy and others.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제33권2호
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• pp.185-189
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• 2011

Tooth impaction represents the stop of eruption by clinical and radiographical disturbance in eruption path or the dislocation of tooth germ. The most common factor in tooth eruption disorders are spacial deficiency with other causes reported to be odontogenic tumors, periodontal ligament injury, etc. Impaction of the mandibular second molar is relatively rare and reported in about 3 out of 1,000 people. Because the second molars tend to erupt in a mesial direction, this situation can lead to serious problems if untreated, including dental caries, periodontal disease and root resorption of the first molar. Treatment of this problem includes, surgical repositioning and orthodontic forced eruption. Because each procedure have the definite advantages and disadvantages, and influenced by circumferential environment, these have limits for successfu1 recovery as independent treatment. In a case at St. Mary's Hospital, we performed successful correction of a horizontal impacted mandibular second molar using a miniplate skeletal anchorage system. We introduce this treatment as a valid method for an impacted second molar and consider a oromaxillofacial surgeon's role in tooth movement treatment.

• Asian-Australasian Journal of Animal Sciences
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• 제25권8호
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• pp.1083-1088
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• 2012

The breeding value of marbling score in skeletal muscle is an important factor for evaluating beef quality. In the present study, we investigated proteins associated with the breeding value of the marbling score for bovine sirloin to select potential biomarkers to improve meat quality through comparative proteomic analysis. Proteins isolated from muscle were separated by two-dimensional gel electrophoresis. After analyzing images of the stained gel, seven protein spots for the high marbling score group were identified corresponding to changes in expression that were at least two-fold compared to the low marbling score group. Four spots with increased intensities in the high marbling score group were identified as phosphoglycerate kinase 1, triosephophate isomerase, acidic ribosomal phosphoprotein PO, and capping protein (actin filament) Z-line alpha 2. Spots with decreased intensities in the high marbling score group compared to the low score group were identified as 14-3-3 epsilon, carbonic anhydrase II, and myosin light chain 1. Expression of myosin light chain 1 and carbonic anhydrase 2 was confirmed by Western blotting. Taken together, these data could help improve the economic performance of cattle and provide useful information about the underlying the function of bovine skeletal muscle.

• Molecules and Cells
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• 제37권9호
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• pp.685-690
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• 2014

Osteoclasts are large polykaryons that have the unique capacity to degrade bone and are generated by the differentiation of myeloid lineage progenitors. To identify the genes involved in osteoclast development, we performed microarray analysis, and we found that carboxypeptidase E (CPE), a prohormone processing enzyme, was highly upregulated in osteoclasts compared with their precursors, bone marrow-derived macrophages (BMMs). Here, we demonstrate a novel role for CPE in receptor activator of NF-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation. The overexpression of CPE in BMMs increases the formation of tartrate-resistant acid phosphatase (TRAP)-positive multinuclear osteoclasts and the expression of c-Fos and nuclear factor of activated T cells c1 (NFATc1), which are key regulators in osteoclastogenesis. Furthermore, employing CPE knockout mice, we show that CPE deficiency attenuates osteoclast formation. Together, our data suggest that CPE might be an important modulator of RANKL-induced osteoclast differentiation.

• Nutrition Research and Practice
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• 제17권4호
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• pp.660-669
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• 2023

BACKGROUND/OBJECTIVES: To investigate the effect and regulatory mechanism of resveratrol supplementation on the mitochondrial energy metabolism of rats with exercise-induced fatigue. MATERIALS/METHODS: Forty-eight Sprague-Dawley male rats were divided randomly into a blank control group (C), resveratrol group (R), exercise group (E), and exercise and resveratrol group (ER), with 12 rats in each group. Group ER and group E performed 6-wk swimming training with 5% wt-bearing, 60 min each time, 6 days a wk. Group ER was given resveratrol 50 mg/kg by gavage one hour after exercise; group R was only given resveratrol 50 mg/kg by gavage; group C and group E were fed normally. The same volume of solvent was given by gavage every day. RESULTS: Resveratrol supplementation could reduce the plasma blood urea nitrogen content, creatine kinase activity, and malondialdehyde content in the skeletal muscle, increase the total superoxide dismutase activity in the skeletal muscle, and improve the fatigue state. Resveratrol supplementation could improve the activities of Ca²⁺-Mg²⁺-ATPase, Na⁺-K⁺-ATPase, succinate dehydrogenase, and citrate synthase in the skeletal muscle. Furthermore, resveratrol supplementation could up-regulate the sirtuin 1 (SIRT1)-proliferator-activated receptor gamma coactivator-1α (PGC-1α)-nuclear respiratory factor 1 pathway. CONCLUSIONS: Resveratrol supplementation could promote mitochondrial biosynthesis via the SIRT1/PGC-1α pathway, increase the activity of the mitochondrial energy metabolism-related enzymes, improve the antioxidant capacity of the body, and promote recovery from exercise-induced fatigue.

• 대한치과교정학회지
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• 제26권4호
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• pp.349-358
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• 1996

부정교합을 치료하기 위해 영구치 발거여부를 결정하는 것은 교정학의 역사상 커다란 논쟁거리였고, 따라서 진단과정에서 사용되는 많은 분석법들과 발치의 기준들이 제시되었다. 이제까지 제시되어 온 발치기준들(Extraction formulas)을 검토해 보면, 치열궁 장경 부조화, 치열의 전방돌출, 그리고 환자의 골격형태에 기초하여 발치를 결정함을 알 수 있다. 이들 중에서 가장 중요하게 고려되어야 할 진단요소는 환자의 골격형태로, 결국 개개 환자의 부정교합은 그 환자의 골격형태 범위 내에서 치료되어야 하기 때문이다. EI(Extraction Index)는 환자의 골격형태를 감별진단 할수 있는 CF를 기본으로 구성되어 있고, CF는 부정교합의 수직적, 수평적 요소를 감별해내는 ODI이와 APDI로 이루어져 있다. 또한 피에는 환자의 안모에 영향을 미치는 절치간 각과 입술의 전후방 위치가 포함되어 있어서 안모형태도 고려되어 있다. 본 연구에서는 정상 교합자로서 골격이 조화를 이루며 좋은 안모를 가지고 있고 교정치료를 받은 적이 없는 11.2 - 14.4 세의 아동을 대상으로 하여 두부계측 방사선 사진을 분석하여 EI에 관련된 항목들을 측정한 결과, 다음과 같은 결론을 얻었다. 1. ODI이는 $73.5^{\circ}$, APDI는 $82.8^{\circ}$, CF는 $156.3^{\circ}$ 였다. 2. 절치간 각은 $123.6^{\circ}$ 였다. 3. E-line에 대한 상순의 거리는 0.0mm, 하순은 1.4mm 였다. 4. Extraction Index는 153.8 였다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제37권
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• pp.41.1-41.6
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• 2015

Cleidocranial dysplasia is an autosomal dominant heritable skeletal disorder. The characteristic features of cleidocranial dysplasia (CCD) may include hypoplasia of the clavicle, delayed closure of frontanelles, late tooth eruption, and other skeletal disorders. This case report describes clinical and radiographic manifestations at the age of 11 and 29 of a CCD patient, investigates the mutation of core-binding factor A1 (CBFA1) based on gene analysis, and illustrates successful oral reconstruction with fixed prosthesis and dental implant after the extraction of multiple teeth.

• 대한치과교정학회지
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• 제15권2호
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• pp.279-289
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• 1985

This study was undertaken to find out the factor highly correlated to the depth of overbite among the skeletal factors of the craniofacial complex using lateral roentgenocephalograms. The subjects cconsited of fifty normal occlusions, sixty Class I malocclusions, sixty Class II division I malocclustions and sixty Class III malocclusions. The results were as follows: 1. Ans-Go-Me angle and lower genial angle showed high correlation to the depth of overbite in the total malocclusion sample. 2. The mean values of Ans-Go-Me angle and lower goinal angle for the normal sample were $49.8^{\circ}\;and\;75.6^{\circ}$, respectively. 3. Ans-Go-Me angle above $56^{\circ}$ or lower gonial angle above $84^{\circ}$ indicated a tendency toward an openbite. Conversely, Ans-Go-Me angle below $48^{\circ}$ or lower goinal angle below $73^{\circ}$ indicated a tendency toward a deepbite.