• Title/Summary/Keyword: Size-based selection

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Genetic diversity and divergence among Korean cattle breeds assessed using a BovineHD single-nucleotide polymorphism chip

  • Kim, Seungchang;Cheong, Hyun Sub;Shin, Hyoung Doo;Lee, Sung-Soo;Roh, Hee-Jong;Jeon, Da-Yeon;Cho, Chang-Yeon
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.11
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    • pp.1691-1699
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    • 2018
  • Objective: In Korea, there are three main cattle breeds, which are distinguished by coat color: Brown Hanwoo (BH), Brindle Hanwoo (BRH), and Jeju Black (JB). In this study, we sought to compare the genetic diversity and divergence among there Korean cattle breeds using a BovineHD chip genotyping array. Methods: Sample data were collected from 168 cattle in three populations of BH (48 cattle), BRH (96 cattle), and JB (24 cattle). The single-nucleotide polymorphism (SNP) genotyping was performed using the Illumina BovineHD SNP 777K Bead chip. Results: Heterozygosity, used as a measure of within-breed genetic diversity, was higher in BH (0.293) and BRH (0.296) than in JB (0.266). Linkage disequilibrium decay was more rapid in BH and BRH than in JB, reaching an average $r^2$ value of 0.2 before 26 kb in BH and BRH, whereas the corresponding value was reached before 32 kb in JB. Intra-population, interpopulation, and Fst analyses were used to identify candidate signatures of positive selection in the genome of a domestic Korean cattle population and 48, 11, and 11 loci were detected in the genomic region of the BRH breed, respectively. A Neighbor-Joining phylogenetic tree showed two main groups: a group comprising BH and BRH on one side and a group containing JB on the other. The runs of homozygosity analysis between Korean breeds indicated that the BRH and JB breeds have high inbreeding within breeds compared with BH. An analysis of differentiation based on a high-density SNP chip showed differences between Korean cattle breeds and the closeness of breeds corresponding to the geographic regions where they are evolving. Conclusion: Our results indicate that although the Korean cattle breeds have common features, they also show reliable breed diversity.

Current status and prospects of chrysanthemum genomics (국화 유전체 연구의 동향)

  • Won, So Youn;Kim, Jung Sun;Kang, Sang-Ho;Sohn, Seong-Han
    • Journal of Plant Biotechnology
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    • v.43 no.3
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    • pp.272-280
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    • 2016
  • Chrysanthemum is one of the top floriculture species with ornamental and medicinal value. Although chrysanthemum breeding program has contributed to the development of various cultivars so far, it needs to be advanced from the traditional phenotype-based selection to marker-assisted selection (molecular breeding) as shown in major cereal and vegetable crops. Molecular breeding relies on trait-linked molecular markers identified from genetic, molecular, and genomic studies. However, these studies in chrysanthemum are significantly hampered by the reproductive, genetic, and genomic properties of chrysanthemum such as self-incompatibility, inbreeding depression, allohexaploid, heterozygosity, and gigantic genome size. Nevertheless, several genetic studies have constructed genetic linkage maps and identified molecular markers linked to important traits of flower, leaf, and plant architecture. With progress in sequencing technology, chrysanthemum transcriptome has been sequenced to construct reference gene set and identify genes responsible for developments or induced by biotic or abiotic stresses. Recently, a genome sequencing project has been launched on a diploid wild Chrysanthemum species. The massive sequencing information would serve as fundamental resources for molecular breeding of chrysanthemum. In this review, we summarized the current status of molecular genetics and genomics in chrysanthemum and briefly discussed future prospects.

Selection of Resistant Rootstock and Development of Overwintering Methods for Control of Crown Gall Disease on Grapevine (거봉의 뿌리혹병 방제를 위한 저항성 대목 선발 및 월동법)

  • Kang, Sung-Su;Park, Sang-Heon;Park, Mun-Kyun;Park, Tae-Jin;Kang, Hee-Wan;Choi, Jae-Eul
    • Research in Plant Disease
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    • v.13 no.2
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    • pp.98-103
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    • 2007
  • Grapevines will experience various types of winter damage. Some winter damages are caused by mechanical injury, freezing temperatures or poor vine vigor. This research was conducted to find out the appropriate control methods through selection of resistant rootstocks and improvement of overwintering methods for the control of crown gall disease on 'Kyoho' grape. The crown gall symptoms were not found when three stock plants of grapevine SO4, 5BB and 3306 were inoculated with $10^4cfu/ml$ of Agrobacterium vitis strains (YK2823, YK3312, LMG259, HKA234). But when they were inoculated with higher concentration $(10^6 cfu/ml)$ of A. vitis, irrespective of stocks plants, crown galls were formed all of them and the gall size was much smaller than that of kyoho. Three stock plants were selected as resistant based on above mentioned. Covering trunks and branches with rice straw and insulating coverlet was the most effective method for prevention of crown gall disease. This treatment minimized the ambient temperature changes on grapevine trees during winter season to $9.6^{\circ}C$ and the normal plant growth was due to the absence of freezing injury.

Geophysical and Geological Exploration of Cobalt-rich Ferromanganese Crusts on a Seamount in the Western Pacific (서태평양 해저산 고코발트 망간각 자원평가를 위한 광역 탐사 방안)

  • Kim, Jonguk;Ko, Young-Tak;Hyeong, Kiseong;Moon, Jai-Woon
    • Economic and Environmental Geology
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    • v.46 no.6
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    • pp.569-580
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    • 2013
  • Co-rich ferromanganese crusts (Fe-Mn crusts) distributed on the seamounts in the western Pacific are potential economic resources for cobalt, nickel, platinum, and other rare metals in the future. Regulations for prospecting and exploration of Fe-Mn crusts in the Area, which enables the process to obtain an exclusive exploration right for blocks of the fixed size, were enacted recently by the International Seabed Authority, which led to public attention on its potential for commercial development. Evaluation and selection of a mining site can be established based on abundance and grade of Fe-Mn crusts in the site as well as topography that should be smooth enough for mining efficiency. Therefore, acquisition of shipboard echo-sounding and acoustic backscatter data are prerequisite to select potential mine sites in addition to visual and sampling operations. Acoustic backscatter data can be used to locate crust-covered areas in a regional scale with the understanding of acoustic properties of crust through its correlation with visual and sampling data. KIOST had collected the topographic and geologic data to assess the resources potential for Fe-Mn crusts in the west Pacific region from 1994 to 2001. However, they could not obtain acoustic backscatter data that is crucial for the selection of prospective mining sites. Therefore, additional exploration surveys are required to carry out side scan sonar mapping combined with seafloor observation and sampling to decide the blocks for application of an exclusive exploration right.

Mature Market Sub-segmentation and Its Evaluation by the Degree of Homogeneity (동질도 평가를 통한 실버세대 세분군 분류 및 평가)

  • Bae, Jae-ho
    • Journal of Distribution Science
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    • v.8 no.3
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    • pp.27-35
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    • 2010
  • As the population, buying power, and intensity of self-expression of the elderly generation increase, its importance as a market segment is also growing. Therefore, the mass marketing strategy for the elderly generation must be changed to a micro-marketing strategy based on the results of sub-segmentation that suitably captures the characteristics of this generation. Furthermore, as a customer access strategy is decided by sub-segmentation, proper segmentation is one of the key success factors for micro-marketing. Segments or sub-segments are different from sectors, because segmentation or sub-segmentation for micro-marketing is based on the homogeneity of customer needs. Theoretically, complete segmentation would reveal a single voice. However, it is impossible to achieve complete segmentation because of economic factors, factors that affect effectiveness, etc. To obtain a single voice from a segment, we sometimes need to divide it into many individual cases. In such a case, there would be a many segments to deal with. On the other hand, to maximize market access performance, fewer segments are preferred. In this paper, we use the term "sub-segmentation" instead of "segmentation," because we divide a specific segment into more detailed segments. To sub-segment the elderly generation, this paper takes their lifestyles and life stages into consideration. In order to reflect these aspects, various surveys and several rounds of expert interviews and focused group interviews (FGIs) were performed. Using the results of these qualitative surveys, we can define six sub-segments of the elderly generation. This paper uses five rules to divide the elderly generation. The five rules are (1) mutually exclusive and collectively exhaustive (MECE) sub-segmentation, (2) important life stages, (3) notable lifestyles, (4) minimum number of and easy classifiable sub-segments, and (5) significant difference in voices among the sub-segments. The most critical point for dividing the elderly market is whether children are married. The other points are source of income, gender, and occupation. In this paper, the elderly market is divided into six sub-segments. As mentioned, the number of sub-segments is a very key point for a successful marketing approach. Too many sub-segments would lead to narrow substantiality or lack of actionability. On the other hand, too few sub-segments would have no effects. Therefore, the creation of the optimum number of sub-segments is a critical problem faced by marketers. This paper presents a method of evaluating the fitness of sub-segments that was deduced from the preceding surveys. The presented method uses the degree of homogeneity (DoH) to measure the adequacy of sub-segments. This measure uses quantitative survey questions to calculate adequacy. The ratio of significantly homogeneous questions to the total numbers of survey questions indicates the DoH. A significantly homogeneous question is defined as a question in which one case is selected significantly more often than others. To show whether a case is selected significantly more often than others, we use a hypothesis test. In this case, the null hypothesis (H0) would be that there is no significant difference between the selection of one case and that of the others. Thus, the total number of significantly homogeneous questions is the total number of cases in which the null hypothesis is rejected. To calculate the DoH, we conducted a quantitative survey (total sample size was 400, 60 questions, 4~5 cases for each question). The sample size of the first sub-segment-has no unmarried offspring and earns a living independently-is 113. The sample size of the second sub-segment-has no unmarried offspring and is economically supported by its offspring-is 57. The sample size of the third sub-segment-has unmarried offspring and is employed and male-is 70. The sample size of the fourth sub-segment-has unmarried offspring and is not employed and male-is 45. The sample size of the fifth sub-segment-has unmarried offspring and is female and employed (either the female herself or her husband)-is 63. The sample size of the last sub-segment-has unmarried offspring and is female and not employed (not even the husband)-is 52. Statistically, the sample size of each sub-segment is sufficiently large. Therefore, we use the z-test for testing hypotheses. When the significance level is 0.05, the DoHs of the six sub-segments are 1.00, 0.95, 0.95, 0.87, 0.93, and 1.00, respectively. When the significance level is 0.01, the DoHs of the six sub-segments are 0.95, 0.87, 0.85, 0.80, 0.88, and 0.87, respectively. These results show that the first sub-segment is the most homogeneous category, while the fourth has more variety in terms of its needs. If the sample size is sufficiently large, more segmentation would be better in a given sub-segment. However, as the fourth sub-segment is smaller than the others, more detailed segmentation is not proceeded. A very critical point for a successful micro-marketing strategy is measuring the fit of a sub-segment. However, until now, there have been no robust rules for measuring fit. This paper presents a method of evaluating the fit of sub-segments. This method will be very helpful for deciding the adequacy of sub-segmentation. However, it has some limitations that prevent it from being robust. These limitations include the following: (1) the method is restricted to only quantitative questions; (2) the type of questions that must be involved in calculation pose difficulties; (3) DoH values depend on content formation. Despite these limitations, this paper has presented a useful method for conducting adequate sub-segmentation. We believe that the present method can be applied widely in many areas. Furthermore, the results of the sub-segmentation of the elderly generation can serve as a reference for mature marketing.

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Prioritized Channel Contention Access Method for TDMA based MAC Protocol in Wireless Mesh Network (WMN에서 TDMA 기반 MAC Protocol을 위한 우선순위 채널 경쟁 접근 방법)

  • Yun, Sang-Man;Lee, Soon-Sik;Lee, Sang-Wook;Jeon, Seong-Geun;Lee, Woo-Jae
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.13 no.9
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    • pp.1883-1890
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    • 2009
  • Existing MAC protocol can not show good performance in WMN environment. New MAC protocols is proposed because of Mobile Point's mobility, entire distributed environment, heavy traffic problems. This thesis proposes new channel contention method fur Mesh DCF. Mesh DCF uses ACH phase in TDMA frame to perform selection and elimination. Prioritized phases's count m and Fair Elimination phases's count n is determine contention level and make string probability to only one win the contention. Contention Number group's count K to determine the contention level in Fair Elimination Phase gives Fairness but make low probability to only one win the contention. It is sure that enough size of n and K can improve entire performance as result.

Genetic diversity analysis of Thai indigenous chickens based on complete sequences of mitochondrial DNA D-loop region

  • Teinlek, Piyanat;Siripattarapravat, Kannika;Tirawattanawanich, Chanin
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.6
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    • pp.804-811
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    • 2018
  • Objective: Complete mtDNA D-loop sequences of four Thai indigenous chicken varieties, including Pra-dhu-hang-dam (PD), Leung-hang-khao (LK), Chee (CH), and Dang (DA) were explored for genetic diversity and relationships with their potential ancestor and possible associates to address chicken domestication in Thailand. Methods: A total of 220 complete mtDNA D-loop sequences of the four Thai indigenous chicken varieties were obtained by Sanger direct sequencing of polymerase chain reaction amplicons of 1,231 to 1,232 base pair in size. A neighbor-joining dendrogram was constructed with reference complete mtDNA D-loop sequences of Red Junglefowl (RJF) and those different chicken breeds available on National Center for Biotechnology Information database. Genetic diversity indices and neutrality test by Tajima's D test were performed. Genetic differences both within and among populations were estimated using analysis of molecular variance (AMOVA). Pairwise fixation index ($F_{ST}$) was conducted to evaluated genetic relationships between these varieties. Results: Twenty-three identified haplotypes were classified in six haplogroups (A-E and H) with the majority clustered in haplogroup A and B. Each variety was in multiple haplogroups with haplogroups A, B, D, and E being shared by all studied varieties. The averaged haplotype and nucleotide diversities were, respectively 0.8607 and 0.00579 with non-significant Tajima's D values being observed in all populations. Haplogroup distribution was closely related to that of RJF particularly Gallus gallus gallus (G. g. gallus) and G. g. spadiceus. As denoted by AMOVA, the mean diversity was mostly due to within-population variation (90.53%) while between-population variation (9.47%) accounted for much less. By pairwise $F_{ST}$, LK was most closely related to DA ($F_{ST}=0.00879$) while DA was farthest from CH ($F_{ST}=0.24882$). Conclusion: All 4 Thai indigenous chickens are in close relationship with their potential ancestor, the RJF. A contribution of shared, multiple maternal lineages was in the nature of these varieties, which have been domesticated under neutral selection.

Improved CNN Algorithm for Object Detection in Large Images

  • Yang, Seong Bong;Lee, Soo Jin
    • Journal of the Korea Society of Computer and Information
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    • v.25 no.1
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    • pp.45-53
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    • 2020
  • Conventional Convolutional Neural Network(CNN) algorithms have limitations in detecting small objects in large image. In this paper, we propose an improved model which is based on Region Of Interest(ROI) selection and image dividing technique. We prepared YOLOv3 / Faster R-CNN algorithms which are transfer-learned by airfield and aircraft datasets. Also we prepared large images for testing. In order to verify our model, we selected airfield area from large image as ROI first and divided it in two power n orders. Then we compared the aircraft detection rates by number of divisions. We could get the best size of divided image pieces for efficient small object detection derived from the comparison of aircraft detection rates. As a result, we could verify that the improved CNN algorithm can detect small object in large images.

A Study on the Possibility of Applying Blended teaming to Design Education - Focused on the survey of learners′ satisfaction - (디자인교육에서 혼합형 수업(Blended learning) 적용 가능성 - 학습자 만족도 조사를 중심으로 -)

  • 백수희
    • Archives of design research
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    • v.16 no.4
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    • pp.443-452
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    • 2003
  • This study is to find out the possibility of applying the Blended Learning which combines the advantage of face-to-face classroom instruction and e-Learning to design education. This Blended Loaming Model has five steps such as 'prepare, teach, show, interact, and collaborate'. It is designed by the learning decision points (performance outcomes, content stability and structure, audience size, and so on) and the characteristics of delivery methods(asynchronous, synchronous). This research is based on the learners' satisfaction data of each step. The number of the participants is 27 and a questionnaire with 21 items is administered. Ten items of them are designed with 5-point Likert scales and the rest of them ask learners describe the reason of their selection. The result show that learners are satisfied with the blended learning than the off-line classroom. It is expected that the blended learning can be a substitute of the off-line classroom for the effectiveness of design education.

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Overestimation of own body weights in female university students: associations with lifestyles, weight control behaviors and depression

  • Kim, Mi-So;Lee, Hong-Mie
    • Nutrition Research and Practice
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    • v.4 no.6
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    • pp.499-506
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    • 2010
  • The study aimed to analyze the lifestyles, weight control behavior, dietary habits, and depression of female university students. The subjects were 532 students from 8 universities located in 4 provinces in Korea. According to percent ideal body weight, 33 (6.4%), 181 (34.0%), 283 (53.2%), 22 (4.1%) and 13 (2.5%) were severely underweight, underweight, normal, overweight and obese, respectively, based on self-reported height and weight. As much as 64.1 % and only 2.4%, respectively, overestimated and underestimated their body weight status. Six overweight subjects were excluded from overestimation group for the purpose of this study, resulting in overestimation group consisting of only underweight and normal weight subjects. Compared to those from the normal perception group, significantly more subjects from the overestimation group were currently smoking (P=0.017) and drank more often than once a week (P=0.015), without any significant differences in dietary habits. Despite similar BMIs, subjects who overestimated their own weight statuses had significantly higher weight dissatisfaction (P= 0.000), obesity stress (P= 0.000), obsession to lose weight (P = 0.007) and depression (P = 0.018). Also, more of them wanted to lose weight (P = 0.000), checked their body weights more often than once a week (P=0.025) and had dieting experiences using 'reducing meal size' (P=0.012), 'reducing snacks' (P=0.042) and 'taking prescribed pills' (P = 0.032), and presented 'for a wider range of clothes selection' as the reason for weight loss (P = 0.039), although none was actually overweight or obese. Unlike the case with overestimating one's own weight, being overweight was associated with less drinking 0.035) and exercising more often (P=0.001) and for longer (P=0.001) and healthier reasons for weight control (P=0.002), despite no differences in frequency of weighing and depression. The results showed that weight overestimation, independent of weight status, is associated with risky lifestyles, weight control behaviors, and mental conditions. Preventive interventions should focus not only on obesity, but also on body weight overestimation.