• 한국토양비료학회지
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• 제45권6호
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• pp.1073-1085
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• 2012

Soil microbial communities are immensely diverse and complex with respect to species richness and community size. These communities play essential roles in agricultural soil because they are responsible for most of the nutrient cycles in the soil and influence the plant diversity and productivity. However, the majority of these microbes remain uncharacterized because of poor culturability. Next-generation sequencing techniques have revolutionized many areas of biology by providing cheaper and faster alternatives to Sanger sequencing. Among them, amplicon pyrosequencing is a powerful tool developed by 454 Life Sciences for assessing the diversity of complex microbial communities by sequencing PCR products or amplicons. This review summarizes the current opinions in amplicon sequencing of soil microbial communities, and provides practical guidance and advice on sequence quality control, aligning, clustering, OTU- and taxon-based analysis. The last section of this article includes a few representative studies conducted using amplicon pyrosequencing.

• 한국지역사회생활과학회지
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• 제24권2호
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• pp.251-258
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• 2013

Korean oil and honey Yukwa has been paid attention as formal cake for traditional national seasons' holiday and religious service. Quality of Yukwa, however, has been maintained arbitrarily by each Yukwa manufacturer. Since even same Yukwa had severe differences in size, weight, and pattern, it has given the negative effect to the consumer. Yukwa industries need to setup the quantitative quality specifications instead of qualitative ones to maintain the uniformity of Yukwa quality. Efficient and economical inspection and process control system should be developed. In developing quality standards of Yukwa, features which can measure quality quantitatively in real time should be properly chosen. Existing quality features such as acidity, oxidization, hardness, viscosity, and texture were measured by the chemical or physical base destructive methods. Many research and developments have been performed in investigating and analyzing chemical transition states of those quality features as environment or storage condition changes. Most methods, however, require either off-line or complex treatment or time consuming process of analysis in evaluating quality features. Consumer, however, selects products mostly based on the external features such as shape, size, and color. Therefore, critical visual quality features should be chosen and the efficient real time measurement system must be developed. In this paper, computer image acquisition and processing system were developed and software modules were developed to extract the quantitative data of those features in real-time. Computer image processing system will promote in maintaining uniform quality of Yukwa and establishing quality standards of Yukwa.

• 한국음향학회지
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• 제35권6호
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• pp.445-451
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• 2016

나노기술의 실제적인 응용을 위해 응집되지 않은 나노입자 상태를 얻는 것이 매우 중요하다. 나노입자를 사용하는 제품의 기능성을 향상시키기 위해, 그들의 합성 과정에서 입자 크기 분포의 더 정밀한 제어가 요구된다. 그러나 합성된 나노입자들은 물리적 혹은 화학적인 이유로 응집되기 쉬워 나노입자의 고유한 특성이 가려져 실제적인 응용에 있어서 문제를 일으킨다. 본 연구는 단분산된 나노입자만을 분리하기 위하여 초음파 무화 효과에 의한 무화입자를 분리장으로 사용한 나노입자 분리방법을 제안하였다. 0.002 wt. %의 농도를 갖는 $TiO_2$ 나노입자 현탁액을 무화시켜 분리포집된 현탁액에 포합된 나노입자들의 입도분포를 측정하였다. 그 결과들로부터 제안된 방법을 이용하여 단분산 입자의 분리 포집이 가능함을 확인 할 수 있다.

• Journal of Biosystems Engineering
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• 제30권3호
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• pp.172-178
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• 2005

Grading and sorting an indeterminate form of agricultural products such as sweet potatoes and potatoes are a labor intensive job because its shape and size are various and complicate. It costs a great deal to sort sweet potato in an indeterminate forms. There is a great need for an automatic grader fur the potatoes. Machine vision is the promising solution for this purpose. The optical indices for qualifying weight and appearance quality such as shape, color, defects, etc. were obtained and an on-line sorting system was developed. The results are summarized as follows. Sorting system combined with an on-line inspection device was composed of 5 sections, human inspection, feeding, illumination chamber, image processing & control, and grading & discharging. The algorithms to compute geometrical parameters related to the external guality were developed and implemented for sorting the deformed sweet potatoes. Grading accuracy by image processing was $96.4\%$ and the processing capacity was 10,800 pieces per hour.

• 한국세라믹학회지
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• 제43권9호
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• pp.564-568
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• 2006

AlO(OH) nano gel is used in precursor of ceramic material, coating material and catalyst. For use of these, not only physiochemical control for particle morphology, pore characteristic and peptization but also studies of synthetic method for preparation of advanced application products were required. In this study, AlO(OH) nano gel was prepared through the aging and drying process of aluminum hydroxides gel precipitated by the hydrolysis reaction of dilute NaOH solution and aluminum sulfate solution. In this process, optimum synthetic condition of AlO(OH) nano gel having excellent pore volume as studying the effect of water and aluminum sulfate mole ratio on gel precipitates has been studied. Water and aluminum sulfate mole ratio brought about numerous changes on crystal morphology, surface area, pore volume and pore size. Physiochemical properties were investigated as using XRD, TEM, TG/DTA, FT-IR, and $N_2$ BET method.

• 한국항해항만학회지
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• 제28권1호
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• pp.43-49
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• 2004

결함검사는 생산공정에 있어서 상품의 디자인과 함께 매우 중요한 부분으로서, 상품의 경쟁력을 높이는데 필수 불가결한 것이다. 만약, 실시간 결함검출이 상품에 대한 어떤 손상도 없이 할 수 있다면, 품질 및 공정의 효율적 관리와 고비용 인력의 절감을 통하여 생산원가를 줄일 수 있다. 본 논문에서는 철판과 같은 표면에 결함이 있는 경우 필요한 정보만을 추출할 수 있는 3가지 공간필터법에 대하여 제안하였고, 공간필터의 특성을 통하여 결함검출 시스템을 구성하였다. 그리고, 최적의 표면결함 계측용 공간필터법을 개발하기 위하여 결함의 크기와 형태, 광도의 크기 및 외부 광간섭 그리고 슬리트의 개수와 같은 파라메타의 변화에 따른 측정 성능을 비교 및 분석하였다.

• 한국재료학회지
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• 제21권6호
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• pp.327-333
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• 2011

Nanostructured cobalt materials have recently attracted considerable attention due to their potential applications in high-density data storage, magnetic separation and heterogeneous catalysts. The size as well as the morphology at the nano scale strongly influences the physical and chemical properties of cobalt nano materials. In this study, cobalt nano particles synthesized by a a polyol process, which is a liquid-phase reduction method, were investigated. Cobalt hydroxide ($Co(OH)_2$), as an intermediate reaction product, was synthesized by the reaction between cobalt sulphate heptahydrate ($CoSO_4{\cdot}7H_2O$) used as a precursor and sodium hydroxide (NaOH) dissolved in DI water. As-synthesized $Co(OH)_2$ was washed and filtered several times with DI water, because intermediate reaction products had not only $Co(OH)_2$ but also sodium sulphate ($Na_2SO_4$), as an impurity. Then the cobalt powder was synthesized by diethylene glycol (DEG), as a reduction agent, with various temperatures and times. Polyvinylpyrrolidone (PVP), as a capping agent, was also added to control agglomeration and dispersion of the cobalt nano particles. The optimized synthesis condition was achieved at $220^{\circ}C$ for 4 hours with 0.6 of the PVP/$Co(OH)_2$ molar ratio. Consequently, it was confirmed that the synthesized nano sized cobalt particles had a face centered cubic (fcc) structure and with a size range of 100-200 nm.

• Journal of Microbiology and Biotechnology
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• 제33권2호
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• pp.211-218
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• 2023

A cryptic plasmid (pTH32) was characterized from Tetragenococcus halophilus 32, an isolate from jeotgal, Korean traditional fermented seafood. pTH32 is 3,198 bp in size with G+C content of 35.84%, and contains 4 open reading frames (ORFs). orf1 and orf2 are 456 bp and 273 bp in size, respectively, and their translation products showed 65.16% and 69.35% similarities with RepB family plasmid replication initiators, respectively, suggesting the rolling-circle replication (RCR) mode of pTH32. orf3 and orf4 encodes putative hypothetical protein of 186 and 76 amino acids, respectively. A novel Tetragenococcus-Escherichia coli shuttle vector, pMJ32E (7.3 kb, Em^r), was constructed by ligation of pTH32 with pBluescript II KS(+) and an erythromycin resistance gene (ErmC). pMJ32E successfully replicated in Enterococcus faecalis 29212 and T. halophilus 31 but not in other LAB species. A pepA gene, encoding aminopeptidase A (PepA) from T. halophilus CY54, was successfully expressed in T. halophilus 31 using pMJ32E. The transformant (TF) showed higher PepA activity (49.8 U/mg protein) than T. halophilus 31 cell (control). When T. halophilus 31 TF was subculturd in MRS broth without antibiotic at 48 h intervals, 53.8% of cells retained pMJ32E after 96 h, and only 2.4% of cells retained pMJ32E after 14 days, supporting the RCR mode of pTH32. pMJ32E could be useful for the genetic engineering of Tetragenococcus and Enterococcus species.

• 한국분말재료학회지
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• 제22권4호
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• pp.234-239
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• 2015

Electronic products are a major part of evolving industry and human life style; however most of them are known to emit electromagnetic waves that have severe health hazards. Therefore, different materials and fabrication techniques are understudy to control or limit transfer of such waves to human body. In this study, nanocomposite powder is dispersed into epoxy resin and shielding effects such as absorption, reflection, penetration and multiple reflections are investigated. In addition, nano size powder (Ni, $Fe_2O_3$, Fe-85Ni, C-Ni) is fabricated by pulsed wire evaporation method and dispersed manually into epoxy. Characterization techniques such as X-ray diffraction, Scanning electron microscopy and Transmission electron microscopy are used to investigate the phase analysis, size and shape as well as dispersion trend of a nano powder on epoxy matrix. Shielding effect is measured by standard test method to investigate the electromagnetic shielding effectiveness of planar materials, ASTM D4935. At lower frequency, sample consisting nano-powder of Fe-85%Wt Ni shows better electromagnetic shielding effect compared to only epoxy, only Ni, $Fe_2O_3$ and C-Ni samples.

• 한국정보과학회논문지:데이타베이스
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• 제35권6호
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• pp.533-543
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• 2008

USB 저장장치는 반도체 메모리 저장 용량에 대한 기순 발전에 따라, 대용량 저장공간을 제공하는 제품으로 생산되고 있다. 따라서 소비자는 저장공간이 상대적으로 작은 기존 USB 저장장치를 폐기하거나, 효율적으로 사용하지 않는다. 본 논문에서는 메모리 저장 용량이 다른, 다수의 USB 저장장치를 USB 허브를 통하여 그룹화 하여 사용자가 컴퓨터에서 논리적 단일 저장공간으로 사용할 수 있는 개선된 USB 허브인 시그마 허브(Sigma Hub)를 제안한다. 제안된 시그마 허브는 다수의 USB 저장장치를 단일화하기 위한 관리 모듈로서 시그마 콘트롤러(Sigma Controller)를 포함한다. 시그마 콘트롤러는 USB 저장장치 통합 알고리즘을 통하여 트랜잭션을 제어하여 논리적 단일 저장장치 사용을 가능하게 한다.