• Journal of the Korean Geographical Society
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• v.40 no.3 s.108
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• pp.274-284
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• 2005

On the subject of Yeoungsan River Yeoungbon c and Seumjin River Seumbon e site discussing as the measuring points of discharge and Quality by the Department of Environment, this study executes the tests of proprieties and examines the methods of flow measuring and assessment. The result of this study may summarize as belows. According to the hydrogeomorphological aspects and artificial effects, Yeoungbon c and Seumbon e site are not proper for the measuring points of the water levels. Also, the methods of river discharge measuring by the specific discharge method, first tried in this study, has an enough reliability which can be used to measure the site where is difficult to measure the flow directly or to select the representative site to measure on the up and downstream. In case of accumulating the specific discharge data throughout the flow observation by seasons and periods for a long time, these may be used to measure the flow as well.

• Journal of Digital Convergence
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• v.2 no.2
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• pp.29-44
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• 2004

The main objective of this study is to investigate the determinants of consumer trust in e-business. It examines consumer perceptions trust in a Web site and address following research questions: What factors influence consumer trust in a Web site and what specific Web site cues are associated with trust and satisfaction? We test our hypothesis in a empirical data from 568 consumers across 4 Web sites. By factor analysis, the results show that Web site characteristics are such as product information and purchase process , system stability, navigation, privacy(security), design, information of seller, pay methods, and customer service. We also find that brand and Web site characteristics such as Web site design, navigation, privacy(security), and customer service can explain over 59% of the variance in Web site trust and 60% in satisfaction. The results offer important implication for Web site strategies that include the manipulation of factors influencing Web site trust. And the future directions of the present research are discussed.

• Journal of the Korean Association of Geographic Information Studies
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• v.19 no.4
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• pp.17-35
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• 2016

Earthquake-induced disasters are often more severe in locations with soft soils than firm soils or rocks due to differences in ground motion amplification. On a regional scale, such differences can be estimated by spatially predicting subsurface soil thickness over the entire target area. In general, soil deposits are generally deeper in coastal or riverside areas than in inland regions. In this study, a coastal metropolitan area, Incheon, was selected to assess site effects and provide information on seismic hazards. Spatial prediction of geotechnical layers was performed for the entire study area within the GIS framework. Approximately 7,000 existing borehole drilling data in the Incheon area were gathered and archived into the GIS Database (DB). In addition, surface geotechnical data were acquired from a walkover survey. Based on the built geotechnical DB, spatial zoning maps of site-specific seismic response parameters were created and presented for use in a regional seismic strategy. Site response parameters were performed to determine site coefficients for seismic design over the entire target area and compared with each other. Site classifications and subsequent seismic zoning were assigned based on site coefficients. From this seismic zonation case study in Incheon, we verified that geotechnical GIS-DB can create spatial zoning maps of site-specific seismic response parameters that are useful for seismic hazard mitigation particularly in coastal metropolitan areas.

• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1799-1808
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• 2006

A food-grade integration vector based on site-specific recombination was constructed. The 5.7-kb vector, pIMA20, contained an integrase gene and a phage attachment site originating from bacteriophage A2, with the ${\alpha}$-galactosidase gene from Lactobacillus plantarum KCTC 3104 as a selection marker. pIMA20 was also equipped with a controllable promoter of nisA ($P_{nisA}$) and a signal peptide-encoding sequence of usp45 ($SP_{usp45}$) for the production and secretion of foreign proteins. pIMA20 and its derivatives mediated site-specific integration into the attB-like site on the Lactococcus lactis NZ9800 chromosome. The vector-integrated recombinant lactococci were easily detected by the appearance of blue colonies on a medium containing $X-{\alpha}-gal$ and also by their ability to grow on a medium containing melibiose as the sole carbon source. Recombinant lactococci maintained these traits in the absence of selection pressure during 100 generations. The ${\alpha}-amylase$ gene from Bacillus licheniformis, lacking a signal peptide-encoding. sequence, was inserted downstream of $P_{nisA}\;and\;SP_{usp45}$ in pIMA20, and the plasmid was integrated into the L. lactis chromosome. ${\alpha}-Amylase$ was successfully produced and secreted by the recombinant L. lactis, controlled by the addition and concentration of nisin.

• International Journal of Highway Engineering
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• v.15 no.2
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• pp.57-63
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• 2013

PURPOSES : This study was performed to investigate a feasibility of job-site use of recycled concrete aggregate exceeding 3% of absorption rate. Test variables are coarse aggregate types such as natural aggregate, job-site processed recycled aggregate, and recycled aggregate processed from the intermediate waste treatment company. METHODS : First, aggregate properties such as gradation, specific gravity and absorption rate were determined. Next a basic series of mechanical properties of concrete was tested. RESULTS : All strength test results such as compression, flexure and modulus were satisfied for the minimum requirements. Finally up to first 48 elapsed days the shrinkage strains of concretes made from both recycled aggregates (in case of volume-surface ratio of 300) appeared to be greater than 26% of the companion concretes made from natural aggregates. CONCLUSIONS : Drying shrinkage result is ascribed to greater absorption rate and specific gravity of those specimens made from recycled aggregate. This may be reduced with an addition of admixtures.

• Journal of Pharmaceutical Investigation
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• v.30 no.2
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• pp.73-83
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• 2000

Polyethylene glycol (PEG) is a water soluble, biocompatible, non-toxic polymer and PEGylation is a well established technique for the modification of therapeutic proteins and peptides. PEG-protein drugs have been extensively studies in relation to therapies for various diseases: cancer, inflammation and others. The covalent attachment of PEG to proteins and peptides prolonged plasma half-life, reduced antigenicity and immunogenicity, increased thermal and mechanical stability, and prevented degradation by enzymes. Several chemical groups for general and site specific conjugation have been exploited to activate PEG for amino group, carboxyl group, and cysteine groups. PEGylation of many proteins and peptides have been studied to enhance their properties for the potential uses. Also, the different positional isomers in several PEG-proteins have shown the difference in vivo stability and biological indicating that the site of PEG molecule attachment is one of the important factor to develop PEG-proteins as potential therapeutic agents.

• Bulletin of the Korean Chemical Society
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• v.14 no.5
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• pp.588-592
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• 1993

The $^1H-NMR$ signals of the heme methyl, propionate and related chemical groups of cytochrome $c_3$ from Desulfovibrio vulgaris Miyazaki F (D.v. MF) were site-specifically assigned by means of 1D-NOE, 2D-DQFCOSY and 2D-TOCSY spectra. They were consistent with the site-specific assignments of the hemes with the highest and second-lowest redox potentials reported by Fan et al. (Biochemistry, 29, 2257-2263 1990). The site-specific heme assignments were also supported by NOE between the methyl groups of these hemes and the side chain of Val-18.

• Journal of Microbiology and Biotechnology
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• v.4 no.4
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• pp.274-277
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• 1994

We subcloned two chicken H3 histone genes and transfected them into Rat 3 cell line. One contains 300 bp 5' to its cap site and the other contains 130 bp 5' to its cap site when cloned into plasm ids. Both of them showed 5' phase specific expression of their mRNA about 8 fold higher (during 5' phase) than during Gl phase. This means that only 130 bp 5' to its cap site was enough to confer cell cycle regulated expression of the latter gene. The DNA sequences of their 5' flanking region did not reveal any particular homologies or subtype-specific sequences. The DNA sequence data also showed that even though the protein coding regions of the histone genes have been conserved exceptionally well throughout evolution, their 5' untranslated regions have not been conserved as well.

• Agricultural and Biosystems Engineering
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• v.7 no.1
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• pp.18-26
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• 2006

In-field site-specific nitrogen (N) management increases crop yield, reduces N application to minimize the risk of nitrate contamination of ground water, and thus reduces farming cost. Real-time N sensing and fertilization is required for efficient N management. An 'on-the-go' site-specific N management system was developed and evaluated for the supplemental N application to com (Zea mays L.). This real-time N sensing and fertilization system monitored and assessed N fertilization needs using a vision-based spectral sensor and controlled the appropriate variable N rate according to N deficiency level estimated from spectral signature of crop canopies. Sensor inputs included ambient illumination, camera parameters, and image histogram of three spectral regions (red, green, and near-infrared). The real-time sensor-based supplemental N treatment improved crop N status and increased yield over most plots. The largest yield increase was achieved in plots with low initial N treatment combined with supplemental variable-rate application. Yield data for plots where N was applied the latest in the season resulted in a reduced impact on supplemental N. For plots with no supplemental N application, yield increased gradually with initial N treatment, but any N application more than 101 kg/ha had minimal impact on yield.

• Journal of Korea Multimedia Society
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• v.23 no.6
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• pp.784-791
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• 2020

A site-specific performance is a genre and a phenomenon that has recently emerged as a recent paradigm of performing arts: a communality. It involves the performativity of the audience and has various phenomena of contemporary performing art. This study aims to figure out methods of performativity. "Lost Missing and Forgotten," which is a collaboration from Finland and Korea created in 2012 and "Trailer," which is held in Copenhagen Denmark in 2010 by Kitt Johnson. Three principles of performativity which have borrowed as critical tools for this study are role reversal, community building, and contact, which based on "The Aesthetics of Performativity," written by Erika Fischer-Lichte in 2017. The study results show that performativity in the vertical structure and horizontal structure of site-specific performance can act as an environmental factor through the audience's body. Additionally, it turned out that role reversal principle was transformation audience to a creator; community building principle led to an interest in neighbors. Lastly, the contact principle was one of the methods to watch the performance.