• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.33 no.2
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• pp.36-48
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• 2015

This study aims to contemplated the enjoying culture of Gyeonghwasejok's garden in late Joseon dynasty. It was track down the behavior from cultural perspective by using recorded in literature. The results were as follows. First, Gyeonghwasejok was the main principal of the garden at Hanyang in Joseon Dynasty. There are established residence in the downtown and make a garden. Garden organizer recognized to fine conditions of residences even crowded downtown. As a result people tried to include habitation and garden culture for preserve their cultural benefit. Secondly, Seongsisanrim culture has appeared of common in site selection of garden for occupies the scenic beauty. Garden was surrounded by scenic beauty. Garden organizer was formed archival culture for owning the beautiful landscape through creation of guguk(九曲), designation of space and lettering on rocks. Thirdly, Formation of the collection culture was placed of various ornaments inside garden. A behaviour of landscape view and ornaments appreciation led to the archival culture such as Won-rim-gi(園林記) and essay(小品文). Moreover, hold a friendship meeting for sharing garden culture. Fourthly, Attention of flowering plants was extended to development of gardening hobby such as fashion of pot-planting, planted to exotic tree. It was know that the plants are recognized as favorite elements by target of appreciation according to introduction of plants inside garden. In addition, facility of horticulture and kitchen garden were placed inside garden. Fifth, Influx of chinese garden culture influenced construction of garden space in late Joseon dynasty. Garden organizer recognizes garden as a ideal space by garden aesthetics that Hojungcheonji(壺中天地). And the imitation of Chinese garden culture such as collecting of Chinese's ornaments has become a high-level culture.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.33 no.2
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• pp.89-102
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• 2015

This study contemplates the tendency of the examination questions for History of Korean Landscape Architecture. The study targets the questions of 'Landscape Architecture History' which has been set in the written test for National Qualification Test of a landscape engineer for recent 10 years from 2005 to 2014 and derives analyzable items based on the guidelines of question-setting presented by Human Resources Development Service of Korea. The results of the study are drawn as follows. First, among 5 areas composing Landscape History, the proportion of Korean Landscape questions is getting increased while that of Western ones is decreasing. Second, about 30 traditional trees and 11 types of traditional landscape elements including traditional facilities were shown in Korean Landscape questions. Besides, history, geographic, practical science, horticulture, anthology books and the 25 tradition landscape-related historic documents categorized as the garden painting data were found. And the kings from ancient era to Choseon Dynasty who were associated with the time of palace garden building, the builders or owners of the villas, the authors of the document and Chinese scholars also appeared. Third, there were no the questions of prehistoric times and Balhae Kingdom, whereas those of Choseon Dynasty were dominantly focused. Among the traditional sites of Choseon Dynasty, Byeolseo(villas) were set most, followed by Dosung or Gung-gweol(castle towns or palaces), houses, Nu Jeong Dea(pavilions) and Seowon(local schools) in order. Nak-an eupseong and Yong-ju sa were the only cases for a castle town and a temple each. Fourth, being associated with tradition spaces, the questions asked for understanding the detailed contents of time of sites' construction, builders, location features, building structures, ground plan types and the components s of garden. In addition, as a result of checking whether traditional landscape sites were shown in the set questions in 9 Korean Landscape textbooks, Dongchundang, Pungamjeonsa, Simgogseowon did not appeared. As a result of reviewing the tendency of the examination questions for History of Korean Landscape Architecture, the questions which ask minor facts without generality and which include difficult information and site uncomprehended in the textbook should be reconsidered.

• Journal of the Korean Association of Geographic Information Studies
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• v.14 no.3
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• pp.236-256
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• 2011

Forest carbon stocks change due to land use change is an important data required by UNFCCC(United Nations framework convention on climate change). Spatially explicit estimation of forest carbon stocks based on IPCC GPG(intergovernmental panel on climate change good practice guidance) tier 3 gives high reliability. But a current estimation which was aggregated from NFI data doesn't have detail forest carbon stocks by polygon or cell. In order to improve an estimation remote sensing and GIS have been used especially in Europe and North America. We divided research trends in main countries into 4 categories such as remote sensing, GIS, geostatistics and environmental modeling considering spatial heterogeneity. The easiest way to apply is combination NFI data with forest type map based on GIS. Considering especially complicated forest structure of Korea, geostatistics is useful to estimate local variation of forest carbon. In addition, fine scale image is good for verification of forest carbon stocks and determination of CDM site. Related domestic researches are still on initial status and forest carbon stocks are mainly estimated using k-nearest neighbor(k-NN). In order to select suitable method for forest in Korea, an applicability of diverse spatial data and algorithm must be considered. Also the comparison between methods is required.

• Journal of Korean Society of Forest Science
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• v.105 no.1
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• pp.19-41
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• 2016

This study examined the flora and community classification in the Forest Genetic Resources Reserves (FGRR) at Mt. Daeseng and Juparyeong to understand the degree of disturbance and the state of forest stand development within the Civilian Control Line (CCL) and to provide baseline data for preservation and management. Of the vascular plants dispersed throughout the study site, there were 98 families, 311 genera, 507 species, 6 subspecies, 65 varieties, and 10 forms, for a total of 588 taxa. Of these, 92 families, 290 genera, 459 species, 6 subspecies, 58 varieties, and 9 forms, for a total of 532 taxa of native plants, were determined to be dispersed around the FGRR at Mt. Daeseng, and 94 families, 259 genera, 364 species, 6 subspecies, 50 varieties, and 8 forms, for a total of 428 taxa of native plants, were confirmed to be dispersed around the FGRR at Juparyeong. There were 21 taxa of endemic Korean plants growing in the FGRR at Mt. Daeseng, with 14 taxa growing in the FGRR at Juparyeong. In terms of rare plants, 26 taxa were confirmed in the FGRR at Mt. Daeseng, and 10 taxa were confirmed in the FGRR at Juparyeong. A Cluster analysis was performed using vegetation data taken from 58 sample plots in each FGRR. The results showed a total of three representative community classifications from Mt. Daeseng: mixed mesophytic forest, Quercus mongolica-Acer pseudosieboldianum, and Quercus mongolica-Fraxinus rhynchophylla communities. Four representative community classifications were observed from Juparyeong: Quercus mongolica-broad leaved forest, Fraxinus rhynchophylla-broad leaved forest, Quercus mongolica, and Quercus communities. On the whole, the species diversity of the communities in Mt. Daeseng FGRR was greater than for the communities in Juparyeong FGRR. At Mt. Daeseng, the mixed mesophytic forest had the highest species diversity index at 1.590, while at Juparyeong, the Fraxinus rhynchophylla-broad leaved forest had the highest species diversity index at 1.319. These study results should serve as useful baseline data for future preservation and management of the demilitarized zone (DMZ) and the surrounding area.

• Economic and Environmental Geology
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• v.40 no.4
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• pp.347-360
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• 2007

In order to evaluate the degree and extent of heavy metal pollution and the environmental impacts of abandoned Cu mines in Goseong-gun, soils and paddies were collected from the mine area and have been analysed for heavy metal contents. The heavy metal contents were much higher in mountain soils than in paddy soils. Total content of heavy metals decreased in order of Cu>Zn>Pb>As>Cr>Cd in mountain soils whereas Zn>Pb>Cu>Cr>As>Cd in paddy soils. The extractable amount of heavy metals by 0.1/1N HCl decreased in order of Cu>Pb>Zn>As>Cd>Cr in mountain soils whereas Pb>Cu>Zn>As>Cd>Cr in paddy soils. Although the extraction ratios were highly various depending on the sampling site, their average values were in order of Cd(16%)>Pb(10%)>Cu(9%)>As(4.5%)>Zn-Cr(${\le}2.5%$). The soils investigated were enriched in heavy metals relative to the averages of earth crust as In order of $As{\ge}Cd$>Pb>Zn>Cu>Cr. Pollution index calculated from total or extractable heavy metals of soils indicated that the heavy metal pollution was restricted to mountain soils around abandoned Cu mines, especially the Samsan I mine. The metal contents of brown rice showed no significantly contaminated level as follows; As $nd{\sim}0.87mg/kg,\;Cd\;0.02{\sim}0.34mg/kg,\;Cu\;1.01{\sim}6.25mg/kg,\;Mn\;13.4{\sim}43.2mg/kg,\;Pb\;0.09{\sim}2.83mg/kg,\;and\;Zn\;16.5{\sim}79.1mg/kg$. From the extraction and dispersion properties of heavy metal with the soil pH ($4.5{\sim}7.8$), it can be deduced the conclusion that the heavy metal pollution is spreading in the study area mainly by the detrital migration of waste ore and gangue minerals rather than the dissolution and circulation of heavy metal.

• Korean Journal of Ecology and Environment
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• v.45 no.4
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• pp.444-452
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• 2012

We conducted a comprehensive monitoring for freshwater food web in a wetland system (Jangcheok Lake), from May to October, 2011. Monthly sampling for zooplankton, fish as well as organic matters, was implemented. In order to understand the food web structure and energy flow, we applied stable isotope analysis to the collected samples, based on ${\delta}^{13}C$ and ${\delta}^{15}N$ values of epiphytic particulate organic matter(EPOM) and particulate organic matter (POM), epiphytic and planktonic zooplankton, fish (Lepomis macrochirus). In the study site, epiphytic and planktonic zooplankton was 24 and 30 species, respectively, and coincidence species between epiphytic and planktonic zooplankton were 20 species. Epiphytic zooplankton were more abundant during the spring and early summer (May to July); however, planktonic zooplankton were more abundant during the autumn (September to October) season. Stable isotope analysis revealed that fish and epiphytic zooplankton had seasonal variations on their food sources. EPOM largely contributed epiphytic zooplankton in spring (May), but increasing contribution of POM in autumn (September) was detected. However, planktonic zooplankton depended on only POM in both seasons. Fish utilized both epiphytic and planktonic zooplankton, but small sized (1~3 cm), fish preferred epiphytic zooplankton, where as larger sized (4~7 cm) fish tended to consume planktonic zooplankton, and epiphytic zooplankton had important role in energy transfer. This pattern was clear when results of spring and autumn stable isotope analysis were compared. From the results of this study, we confirmed that wetlands ecosystem supported various epiphytic and planktonic zooplankton species, they depend on other food items, respectively. L. macrochirus also showed a difference of food source according to the body size, they depend on seasonal density change of zooplankton. In particular, epiphytic zooplankton was very important for growth and development of young fish in the spring.

• Journal of Embryo Transfer
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• v.16 no.3
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• pp.213-221
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• 2001

This study was conducted to examine in vitro developmental ability of porcine embryos after somatic cell nuclear transfer. The porcine ear fell was cultured in vitro for confluency in serum-starvation condition(TCM-199 + 0.5% FBS) far 3~6 days of cell confluency. The zona pellucida of IVM oocytes were partially drilled using laser system. Single somatic cell was individually transferred into enucleated oocytes. And the reconstructed embryos were electrically fused(single DC 1.9kv/cm, 30$\mu$ sec) with 0.3M mannitol. After electrofusion, embryos were activated(single AC 5v/mm, 5sec) and cultured in HCSU-23 medium containing 10% FBS at 39$^{\circ}C$, 5% $CO_2$ in air for 6 to 8 days. The fusion rate of donor cells was 45.6, 36.8 and 46.1% in 3~4, 5~6 days of serum starvation and non serum starvation(N-S), and were 52.7. 53.0 and 51.7% in 1~2. 5~6 and 13~14 passages of donor cell culture, respectively. No significant difference was found in the fusion rate of donor cells by the duration of serum starvation treatment or the number of donor cell passages. By the size of donor cells, however, the fusion rate was significantly higher(P<0.05) for reconstructed embryos derived from 25r $\mu$m $\geq$ site of donor cells (65.3%) than that of 25~30$\mu$ m(42.5%) or 30$\mu$ m(45.5%)$\leq$ cells. The cleavage rate was significantly (P<0.05) higher in 3~4 darts of serum starvation treatment(67.1%) than that in N-S (50.7%) or 5~6 days of starvation(57.1%). The activation rate by the size of donor cells in fused oocytes was 56.5, 68.8 and 58.5%, respectively, and was not significant.

• Korean journal of food and cookery science
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• v.26 no.5
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• pp.603-616
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• 2010

Based on recent dramatic increases in foodborne outbreaks in restaurants, self-managed sanitation systems are now recommended to control contributing risk factors. This study aimed to improve sanitation management practices in restaurants and had two objectives. First, we tried to develop a self-managed sanitation check-list, including risk factors contributing to foodborne illness and Korean food hygiene regulation articles. We also tried to evaluate current sanitation management practices in restaurants according to operation and restaurant type. Thirty restaurants were evaluated by on-the-spot inspectors using an auditing tool consisting of four dimensions, seventeen categories, and forty-one items. Total compliance rate categorized by operation type significantly differed between chain restaurants and self-managed restaurants, with values of 85.5% and 51.6%, respectively. Therefore, self-managed restaurants, which showed the lowest compliance rate of below 30.0%, need more strict control to improve current unsanitary management practices, specifically relating to 'sterilization of knives, chopping boards, and wiping cloths', 'sanitation training', 'not allowing access into the kitchen to outsiders', 'handling of food or utensils on shelves at a 15 cm distance away from floor', 'prevention of cross-contamination of cooked foods or vegetables', and 'records of kitchen access or inspection'. Thus, an effective food sanitation system is essential and should be implemented to improve the existing sanitary conditions in restaurants. However, the most important factor to achieving food sanitation management objectives is food handlers' self-motivation.

• Korean Journal of Plant Tissue Culture
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• v.24 no.1
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• pp.1-35
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• 1997

Fertilized egg, by successive cell divisions, differentiates into different tissues and organs with various structures and functions. Different cells and tissues contain different proteins, products of selective gene expression. Not all the genes in any genomes are equally active, temporal and spatial gene expression being the general rule. Present paper attempts to review the tanscriptional mechanisms or the initiations of transcription from several angles. In some of the organisms the genes in the process of transcription or the genes in the inactive state can be seen under the light microscope. Some bands of Drosophila polytene chromosomes may exhibit a swollen or puff appearance under certain conditions. A puff, unfolded or decondensed form of chromomere, represents sets of intense transcriptional activity or RNA synthesis. The heterochromatic X chromosome whose genes remain inactive in the female mammals can be visualized as a dark staining structure called Barr body, Configuration of chromatin differs between transcribed and nontranscribed chromatin. Modification to the chromatin facilitates RNA synthesis. The movement of large polymerase molecule along the DNA would probably be facilitated if some modifications of the chromatin configuration is effected. Methylation of cytosines in CG sequences is associated with inactive genes. Methylation can play a role in determination of mammalian cells during embryogenesis. Demethylation is necessary for the gene to be expressed during development A histone modification that is also known to be correlated with transcriptional capacity of chromatin is acetylation of the lysine residues of the core histones. Chromatin containing a high level of histone acetylation is very sensitive to DNase 1. For the transcription to occur TBP must first bind to the TATA box. Another TF, TF IIB, then binds to the promoter-TBP complex, facilitating the access of RNA polymerase to the transcription initiation site. As recently as eight years ago researchers assumed that histones were irrelevant to the regulation of gene expression. Histones combine with the DNA to form nucleosome of the chromatin. Histones are vital participant in gene regulation. Histone and basal factors compete for access to TATA box. When DNA is exposed to basal factors before histones are introduced, the basal factors assemble on TATA boxes preventing the access of histones, allowing transcription to occur, for transcription to begin, activator protein at the upstream activation sequence or enhancer must interact with the tail of histone H4 at TATA box and cause the histone role particle to dissociate from the TATA box leading to partial breakup of the histone core particle and allowing the basal factors to bind to the TATA box. New concept of genomic flux in contrast to the old concept of static genome has been developed based on the powerful new molecular techniques. Genomic changes such as repetitive DNAs and transposable elements, it is assumed but not yet proved, may affect some of the developmental patterns that characterize particular cells, tissues, organs, and organisms. In the last decade or so remarkable achievement have been made in the researches of the structures and functions of TFs and the specific target sequences located in promoters or enhancers where these TFs bind. TFs have independent domains that bind DNA and that activate transcription. DNA binding domain of TFs serves to bring the protein into the right location. There are many types of DNA binding domains. Common types of motifs can be found that are responsible for binding to DNA. The motifs are usually quite short and comprise only a small part of the protein structure. Steroid receptors have domains for hormone binding, DNA binding, and activating transcription. The zinc finger motif comprises a DNA binding domain. Leucine zipper consist of a stretch of amino acids with a leucine residue in every seventh position Two proteins form a dimer because they interact by means of leucine zippers on similar α-helical domain. This positions their DNA binding basic domains for interaction with the two halves of a DNA sequence with dyad symmetry of TGACTCA, ACTGAGT.

• Korean Journal of Microbiology
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• v.47 no.3
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• pp.200-208
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• 2011

Most problematic antibiotic resistance mechanism for MLS (macrolide-lincosamide-streptogramn B) antibiotics encountered in clinical practice is mono- or dimethylation of specific adenine residue at 2058 (E. coli coordinate) of 23S rRNA which is performed by Erm (erythromycin ribosome resistance) protein through which bacterial ribosomes reduce the affinity to the antibiotics and become resistant to them. ErmSF is one of the four gene products produced by Streptomyces fradiae to be resistant to its own antibiotic, tylosin. Unlike other Erm proteins, ErmSF harbors idiosyncratic long N-terminal end region (NTER) 25% of which is comprised of arginine well known to interact with RNA. Furthermore, NTER was found to be important because when it was truncated, most of the enzyme activity was lost. Based on these facts, capability of NTER peptide to inhibit the enzymatic activity of ErmSF was sought. For this, expression system for two different proteins to be expressed in one cell was developed. In this system, two plasmids, pET23b and pACYC184 have unique replication origins to be compatible with each other in a cell. And expression system harboring promoter, ribosome binding site and transcription termination signal is identical but disparate amount of protein could be expressed according to the copy number of each vector, 15 for pACYC and 40 for pET23b. Expression of NTER peptide in pET23b together with ErmSF in pACYC 184 in E. coli successfully gave more amounts of NTER than ErmSF but no inhibitory effects were observed suggesting that there should be dynamicity in interaction between ErmSF and rRNA rather than simple and fixed binding to each other in methylation of 23S rRNA by ErmSF.