• BMB Reports
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• 제37권4호
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• pp.474-479
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• 2004

The expressed sequence tag (EST) effort in Toxoplasma gondii has generated a substantial amount of gene information. To exploit this valuable resource, we chose to study tgd057, a novel gene identified by a large number of ESTs that otherwise show no significant match to known sequences in the database. Northern analysis showed that tgd057 is transcribed in this tachyzoite. The complete cDNA sequence of tgd057 is 1169 bp in length. Sequence analysis revealed that tgd057 possibly adopts two polyadenylation sites, utilizes the fourth in-frame ATG for translation initiation, and codes for a secretory protein. The longest open reading frame for the tgd057 gene was cloned and expressed as a recombinant protein (rd57) in Escherichia coli. Western analysis revealed that serum against rd57 recognized a molecule of ~21 kDa in the tachyzoite protein extract. This suggests that the tgd057 gene is expressed in vivo in the parasite.

• 대한토목학회논문집
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• 제34권5호
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• pp.1489-1504
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• 2014

본 논문은 국내 서남해안 해상풍력발전 하부기초설계 시 신뢰성 기반의 한계상태설계법 적용을 위한 말뚝기초의 부분안전계수를 제안하고자 한다. 우선, 해상풍력관련 국제 설계기준서인 IEC, GL, DNV, API, ISO, EUROCODE 등에서 제시한 다양한 부분안전계수 및 저항계수를 비교 분석하였다. 그리고 부안-영광 및 새만금 해역의 서남해안 해상풍력단지의 지반조사 결과를 가지고 불확실성 분석을 수행하였다. 불확실성 분석 결과를 토대로 신뢰성해석을 수행하여 지반정수에 대한 부분안전계수를 산정하였으며, 서남해안 지역의 해상풍력 말뚝기초의 부분안전계수는 1.3으로 평가되었다. 본 논문에서 제안된 부분안전계수는 서남해안에서의 지반조사 결과를 근거로 산정된 것이므로 기타 광역단위 해역에 활용하기 위해서는 추가적인 조사 및 지반정수에 대한 불확실성 분석을 통해 보정이 필요하다고 판단된다.

• Tribology and Lubricants
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• 제35권2호
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• pp.132-138
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• 2019

In this study, we demonstrate the effects of the acidic properties of montmorillonite (MMT), which is commonly used as a catalyst, on the conversion and selectivity of the dimer acid methyl ester (DAME) synthesis. We synthesize DAME by the dimerization of conjugated linoleic acid methyl ester (CLAME) and oleic acid methyl ester using MMT KSF. Incidentally, trimer acid methyl ester was formed as a by-product during the DAME synthesis. There is a necessity to adequately adjust the strength and quantity of the acid site to control the selectivity of DAME. Therefore, we vary the pH of the MMT acid by using various metal hydroxides. The purpose of this study is to increase the yield of monocyclic dimer acid methyl ester, which is a substance with adequate physical properties for industrial applications (e.g., lubricant and adhesive, etc.), using a heterogeneous catalyst. We report the dimerization of fatty acid methyl ester by using base treated-KSF, and apply it to conjugated soybean oil methyl ester. Then, we transmute the acid site properties of KSF, such as pH of 5 wt.% slurry KSF and various alkali metals (Li, Na, K, Ca). Characterization of base treated-KSF using a pH meter, x-ray diffraction, inductively coupled plasma-atomic emission spectrometer, Brunauer-Emmett-Teller surface analysis, and temperature-programmed desorption. We conduct an analysis of CLAME and DAME using nuclear magnetic resonance spectroscopy, gas chromatography, and gel permeation chromatography. Through these experiments, we demonstrate the effects of the acidic properties of KSF on the conversion and selectivity of the DAME synthesis, and evaluate its industrial potential by application to waste vegetable oil.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2000년도 봄 학술발표회 논문집
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• pp.273-280
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• 2000

In recent years there has been a steady increase in geoenvironmental engineering projects where geotechnical engineering has been combined with environmental concerns. Many of these projects involve some investigation of contaminant in the ground. There are many techniques such as geophysical, drilling, sampling, md pushing techniques for investigation of contaminated ground. The most rapidly developing site characterization techniques for geoenvironmental purposes involve direct push technology, that is, penetration tests. The purpose of this study investigated underground oil storage tanks(USTs) using the envi-cone penetrometer system. The electrical resistivity sensor, pH sensor, ORP sensor, and thermometer are installed in envi-cone penetrometer system. This envi-cone penetrometer system provides a continuous profile of measurements, and it is rapid, repeatable, reliable and cost effective for investigation of contaminated ground surrounding the underground oil storage tanks.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 1999년도 가을 학술발표회 논문집
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• pp.247-254
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• 1999

In recent years there has been a steady increase in geoenvironmental engineering projects where geotechnical engineering has been combined with environmental concerns. Many of these projects involve some investigation of contaminant in the ground. There are many techniques such as geophysical, drilling, sampling, and pushing techniques for investigation of contaminated ground. The most rapidly developing site characterization techniques for geoenvironmental purposes Involve direct push technology, that is, penetration tests. In this study, the envi-cone penetrometer system is developed by modification of traditional cone penetration test. The electrical resistivity sensor, pH sensor, thermometer are installed in envi-cone penetrometer system. This envi-cone penetrometer system provides a continuous profile of measurements, and it is rapid, repeatable, reliable and cost effective.

• Journal of Electrical Engineering and Technology
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• 제6권6호
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• pp.836-841
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• 2011

Plasma polymerized methyl methacrylate (ppMMA) thin films were deposited by plasma polymerization technique with different plasma powers and subsequently thermally treated at temperatures of 60 to $150^{\circ}C$. To find a better ppMMA preparation technique for application to organic thin film transistor (OTFT) as dielectric layer, the chemical composition, surface morphology, and electrical properties of ppMMA were investigated. The effect of ppMMA thin-film preparation conditions on the resulting thin film properties were discussed, specifically O-H site content in the pMMA, dielectric constant, leakage current density, and hysteresis.

• 한국안전학회지
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• 제30권3호
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• pp.20-25
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• 2015

It is predicted the mass retirement of the post-war generation and the lack of young people according to reduces the recruitment. Therefore, industry fields are concerned by the low level of occupational safety and health from issued problem in a variety of industries; the charge of expanding business range/multi-functional, black box of technology, difficulty of systematic training, relative decrease in the skill of workers, loss of know-how in the field of information followed restricted site information. In response to these problems, it is necessary to establish the long-termly and actively based on for the adoption of a safety and health management techniques utilizing IT, which is digital assistant(tablet PC, PDA, etc.), RFID/USN/ICT, database systems, and etc. In this study, we developed and evaluated a worker's safety sensing apparatus using smart phone. The apparatus may be useful to prevent accidents in the construction industry as well as confined space work.

• 분석과학
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• 제15권1호
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• pp.36-42
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• 2002

부식질 농도가 매우 높은 지하수로 부터 humic acid (HA) 와 fulvic acid (FA)를 분리 정제한 후 각각의 물리 화학적 특성을 조사하였다. 원소분석, 자외선/가시광선과 적외선 스펙트럼분석 및 $^{13}C-NMR$ 분광법으로 HA 와 FA의 구조 및 작용기를 확인하였다. 또한 양성자 교환능력과 분자 크기분포 측정 실험을 통하여 HA와 FA의 특성 외에 산출지 특성을 나타내며, 상당한 양성자 교환능력을 갖는 다분산계임을 확인하였다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 제38회 하계학술대회
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• pp.1972-1973
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• 2007

A field-oriented UHF system for on-line PD monitoring of transformers is designed, which has been installed inside the oil tank of a transformer. This system has successfully captured long intermittent discharge signals that hadn't been detected through conventional techniques, and solved the problem successfully. The results demonstrate that UHF technique has great advantages for on-line PD monitoring of transformers. By adopting the peak detection technique, it becomes easy and effective for the transplantation of the phase-resolved pattern recognition technique from conventional method to UHF method, and then to realize continuous on-line monitoring, source characterization and trending analysis.

• 한국자기공명학회논문지
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• 제22권4호
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• pp.144-148
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• 2018

Catalytic enzyme Pyrazinamidase (PncA) from Mycobacterium tuberculosis can hydrolyze substrate pyrazinamide (PZA) to pyrazoic acid (POA) as active form of compound. Using NMR spectroscopy, pH-dependent catalytic properties were monitored including metal binding mode during converting PZA to POA. There seems to be a conformational change through zinc binding in active site from the perturbation of peak intensities in series of 2D HSQC spectra the conformation changes through zinc binding.