• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2005년도 춘계 학술발표회 논문집
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• pp.1118-1125
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• 2005

In this study, seismic safety of CFRD(Concrete-Face Rockfill Dam) type "D" dam in operation is evaluated from the results of 1-g shaking table test using similitude laws. Model dam is made by similitude law considering the grain size of prototype dam component. After the model dam is impounded to the normal water level(N.W.L), it is excited by artificial earthquake wave corresponding to standard design respond spectrum of the "D" dam site. Displacement response behavior of the dam is examined through the measurement of vertical and horizontal displacement of dam crest. Also, amplification characteristics of acceleration with dam height is examined through the measurement of acceleration with dam height. Finally, the purpose of this study is to evaluate seismic safety of "D" dam in operation. From the results of acceleration measurement, it was found that acceleration of dam crest was amplified about 1.52 times compared to the acceleration of dam bottom and amplification phenomenon is outstanding at three quarters of dam height from the bottom of dam. From the analysis of displacement behavior, it was estimated that vertical displacement of prototype dam is 6.8cm (0.1% of dam height) and horizontal displacement 12.3cm(0.2% of dam height). These percentages is much lower than 1% of dam height(general stability criteria). Therefore, it was concluded that seismic stability of "D" dam against an estimated earthquake is guaranteed.

• The Plant Pathology Journal
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• 제38권6호
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• pp.665-672
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• 2022

Cymbidium mosaic virus (CymMV) is one of economically important viruses that cause significant losses of orchids in the world. In the present study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay combined with a lateral flow immunostrip (LFI) assay was developed for the detection of CymMV in orchid plants. A pair of primers containing fluorescent probes at each terminus that amplifies highly specifically a part of the coat protein gene of CymMV was determined for RT-RPA assay. The RT-RPA assay involved incubation at an isothermal temperature (39℃) and could be performed rapidly within 30 min. In addition, no cross-reactivity was observed to occur with odontoglossum ringspot virus and cymbidium chlorotic mosaic virus. The RT-RPA with LFI assay (RT-RPA-LFI) for CymMV showed 100 times more sensitivity than conventional reverse transcription polymerase chain reaction (RT-PCR). Furthermore, the RT-PCR-LFI assay demonstrated the simplicity and the rapidity of CymMV detection since the assay did not require any equipment, by comparing results with those of conventional RT-PCR. On-site application of the RT-RPA-LFI assay was validated for the detection of CymMV in field-collected orchids, indicating a simple, rapid, sensitive, and reliable method for detecting CymMV in orchids.

• 보존과학연구
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• 통권22호
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• pp.27-40
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• 2001

DNA typing is often used to determine identity from human remains. Recently, the molecular biological analysis of ancient deposits has become possible since methods for the recovery of DNA conserved in bones or teeth from archaeological remains have been developed. In the field of archaeology, one of the most promising approaches is to identify the individuals present in a mass burial site. We performed nuclear DNA typing and mitochondrial DNA sequencing analysis based on PCR from a Korea ancient human remain excavated from Sa-chon Nuk-island and civilian access controlline(CACL). A femur bone were collected and successfully subjected to DNA extraction, quantification, PCR amplification, and subsequently typed for several shot tandem repeat(STR)loci. 4 types of STR systems used in this study were CTT multiplex(CSF1PO, TPOX, TH01), FFv multiplex(F13A01, FESFPS, vWA), Silver STRⅢ multiplex(D16S539, D7S820, D13S317), and amelogenin for sex determination. This studies are primarily concerned with the extraction, amplification, and DNA typing of ancient human bone DNA samples. Also, it is suggestive of importance about closely relationship between both fields of archaeology and molecular biology.

• Earthquakes and Structures
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• 제19권1호
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• pp.1-16
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• 2020

This study presents an investigation of the dynamic interactions between a surface structure lying on two different soil deposits and a square-shaped buried structure embedded in the soil. To this end, a large number of numerical models are generated by using a well-known Finite Element Method software, i.e., OpenSEES. The interaction phenomenon is assumed to be affected by six different parameters. In the parametric study, these parameters are assumed to have various values in accordance with the engineering practices. A total of 1620 possible combinations of the parameter values are addressed in this study. 30 different numerical models are also generated as the 'free-field cases' to set a reference. The surface structure drift and acceleration amplifications are used as a measure to evaluate the dynamic interactions. The response (i.e., drifts and accelerations) amplifications are calculated as the ratio of the maximum surface structure response in any 'case' to the maximum surface structure response in corresponding free-field case. Variation of the response amplifications with any of the investigated parameters is addressed in this paper. The results obtained from the numerical analyses clearly reveal that the presence of a buried structure in the vicinity of a surface structure can cause both amplification and de-amplification of the surface structure responses, depending on the case parameters.

• Journal of Gastric Cancer
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• 제12권1호
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• pp.7-12
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• 2012

More than ten years have passed since the sentinel node (SN) concept for gastric cancer surgery was first discussed. Less invasive modified surgical approaches based on the SN concept have already been put into practice for malignant melanoma and breast cancer, however the SN concept is not yet placed in a standard position in gastric cancer surgery even after two multi-institutional prospective clinical trials, the Japan Clinical Oncology Group trial (JCOG0302) and the Japanese Society for Sentinel Node Navigation Surgery (SNNS) trial. What is the problem in the clinical application of the SN concept to gastric cancer surgery? There is no doubt that we need reliable indicator(s) to determine with certainty the absence of metastasis in the lymph nodes in order to avoid unnecessary lymphadenectomy. There are several matters of debate in performing the actual procedure, such as the type of tracer, the site of injection, how to detect and harvest, how to detect metastases of SNs, and learning period. These issues have to be addressed further to establish the most suitable procedure. Novel technologies such as indocyanine green (ICG) fluorescence imaging and one-step nucleic acid amplification (OSNA) may overcome the current difficulties. Once we know what the problems are and how to tackle them, we can pursue the goal.

• 한국항만학회지
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• 제6권2호
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• pp.43-64
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• 1992

This study discusses the interacting with deep water waves approaching from deep water based on the linear wave theory and steep sloping sea bottom floor by the numerical procedure. The results of particular interest are particle velocity and acceleration in x, y, z direction wave height amplification factor reflection coefficient and dimensionless pressure distribution on the steep sloping bottom with respect to the various incident wave angle. The wave loads relative to various bottom slopes, incident wave angles and wave periods on submerged breakwater and pipe are represented in comparison with mild sloping bottom the wave load parameters on the steep sloping bottom seemed to be influenced by variation of incident wave angle. In general the particle velocities and accelerations in x, y, z directions on the steep sloping bottom represented larger value or about two than those on the mild sloping bottom according to incident wave angle. However, the wave height amplification factors did not show distinct difference, but the slight variation with respect to the various incident angle showed on mild sloping bottom. The reflection coefficient increased with respect to increase of the incident angle on the steep sloping bottom the results also indicate that the very steep sloping beach produces a rather substantial amount of reflection as we expected. No significant variation of wave pressure was shown on the steep sloping bottom but it represented a certain amount of variation on the mild sloping bottom according to the various incident wave angle. The analysis at the OTEC site also showed similar results.

• Journal of Microbiology and Biotechnology
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• 제34권3호
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• pp.681-688
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• 2024

The accurate and rapid detection of methicillin-resistance of Staphylococcus aureus (SA) holds significant clinical importance. However, the methicillin-resistance detection strategies commonly require complicated cell lysis and gene extraction. Herein, we devised a novel colorimetric approach for the sensitive and accurate identification of methicillin-resistance of SA by combining allosteric probe-based target recognition with self-primer elongation-based target recycling. The PBP2a aptamer in the allosteric probe successfully identified the target MRSA, leading to the initiation of self-primer elongation based-cascade signal amplification. The peroxidase-like hemin/G-quadruplex undergo an isothermal autonomous process that effectively catalyzes the oxidation of ABTS2- and produces a distinct blue color, enabling the visual identification of MRSA at low concentrations. The method offers a shorter duration for bacteria cultivation compared to traditional susceptibility testing methods, as well as simplified manual procedures for gene analysis. The overall amplification time for this test is 60 min, and it has a detection limit of 3 CFU/ml. In addition, the approach has exceptional selectivity and reproducibility, demonstrating commendable performance when tested with real samples. Due to its advantages, this colorimetric assay exhibits considerable potential for integration into a sensor kit, thereby offering a viable and convenient alternative for the prompt and on-site detection of MRSA in patients with skin and soft tissue infections.

• Structural Engineering and Mechanics
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• 제44권5호
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• pp.663-680
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• 2012

Previous major earthquakes revealed that most damage of the buried segmented pipelines occurs at the joints of the pipelines. It has been proven that the differential motions between the pipe segments are one of the primary reasons that results in the damage (Zerva et al. 1986, O'Roueke and Liu 1999). This paper studies the combined influences of ground motion spatial variations and local soil conditions on the seismic responses of buried segmented pipelines. The heterogeneous soil deposits surrounding the pipelines are assumed resting on an elastic half-space (base rock). The spatially varying base rock motions are modelled by the filtered Tajimi-Kanai power spectral density function and an empirical coherency loss function. Local site amplification effect is derived based on the one-dimensional wave propagation theory by assuming the base rock motions consist of out-of-plane SH wave or combined in-plane P and SV waves propagating into the site with an assumed incident angle. The differential axial and lateral displacements between the pipeline segments are stochastically formulated in the frequency domain. The influences of ground motion spatial variations, local soil conditions, wave incident angle and stiffness of the joint are investigated in detail. Numerical results show that ground motion spatial variations and local soil conditions can significantly influence the differential displacements between the pipeline segments.

• 한국지진공학회논문집
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• 제17권5호
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• pp.209-217
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• 2013

In order to verify the reliability of numerical site response analysis program, both soil free-field and base rock input motions should be provided. Beside the field earthquake motion records, the most effective testing method for obtaining the above motions is the dynamic geotechnical centrifuge test. However, need is to verify if the motion recorded at the base of the soil model container in the centrifuge facility is the true base rock input motion or not. In this paper, the appropriate input motion measurement method for the verification of seismic response analysis is examined by dynamic geotechnical centrifuge test and using three-dimensional finite difference analysis results. From the results, it appears that the ESB (equivalent shear beam) model container distorts downward the propagating wave with larger magnitude of centrifugal acceleration and base rock input motion. Thus, the distortion makes the measurement of the base rock outcrop motion difficult which is essential for extracting the base rock incident motion. However, the base rock outcrop motion generated by using deconvolution method is free from the distortion effect of centrifugal acceleration.

• 한국지진공학회논문집
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• 제13권3호
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• pp.29-38
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• 2009

지진 발생시 교량 상판 연결부의 상대변위로 레일에 발생하는 부가응력으로 인한 레일의 좌굴과 파손은 열차의 탈선을 유발할 수 있다. 교량의 지진응답 증가에 영향을 미치는 지반의 탈선취약요소와 교량의 구조적인 요소를 고려하여 고속철도교량 장대레일의 지진응답을 평가하였다. 연약층이 있는 지반, 상하역전형 지반과 같은 지반 탈선취약요소를 고려하기 위해서 지반을 평행층상지반으로 모델링하여 부지효과가 고려된 자유장운동을 구하고 이를 입력지진으로 사용하였다. 교각 높이나 적용 도상을 변화시키면서 구조적 특성이 레일의 지진응답에 미치는 영향을 분석하였다.