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채영규
- The Microorganisms and Industry
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- v.14 no.3
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- pp.7-11
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- 1988
이글에서는 pet A, B, C에 대한 일차적인 아미노산 배열을 알았고 여기서 우리는 위의 정보들을 토대로하여 R. capsulatus에 대한 site-specific oligonucleotide-directed mutagenesis 등 여러 연구를 할 수가 있을 것이다.
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Kim, Ran-Soo
- Journal of architectural history
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- v.26 no.1
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- pp.71-82
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- 2017
Pavilions, follies, and installations provide a place with a new experience beyond that of simple garden architecture. From this point of view, this study tried to analyze the constructs in the Glass House site, which Philip Johnson has built for 50 years. After Chapter 1 Introduction, which summarized the background of the study, Chapter 2 investigated the design background of the landscape and the types of the constructs there. It also, studying literature on pavilions, follies, and installations, defined the basic meanings of them. Chapter 3 identified the features of each construct through the case studies of it, analyzing Johnson's intentions on it. These features are such as the applications of classical follies, the quotations of architectural history, fusion with art, architectural experiments, and the monuments of personal history. In conclusion, this study, finding the site specificity as a common feature of pavilions, follies, and installations, referred to two aspects of this, which are not only physical placeness but also cultural media.
https://doi.org/10.7738/JAH.2017.26.1.071 인용 PDF KSCI

Kim, Myungjin
- Journal of Environmental Impact Assessment
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- v.12 no.3
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- pp.151-160
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- 2003
Analysis of carrying capacity and site suitability by the systematic application of environmental information an provide a basis for implementing environmentally sensitive and sustainable resource development policies. Based on the availability of public services and environmental and site-specific constraints, a Rural Residential Density Matrix was applied in Santa Cruz County, California, USA to assess the development potential of rural land parcels since 1980. In one case, the Matrix was applied to calculate the allowable development density on a site in Lompico planned for use as a Buddhist retreat center. These calculations revealed higher environmental sensitivity and lower carrying capacity than projected in the proposed plan. Under the constraints of the county's minimum acreage policies and insufficient developable land, the developer cancelled the proposed plan. This example suggests that application of the Matrix at the concept development stage would increase the effectiveness of the prior environmental review and environmental impact assessment (EIA) system in Korea.
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Park, Sung-Joon;Cho, Young-Dong
- BMB Reports
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- v.33 no.1
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- pp.69-74
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- 2000
The Arabidopsis thaliana S-Adenosylmethionine decarboxylase (AdoMetDC) cDNA ($GenBank^{TM}$ U63633) was cloned, then the AdoMetDC protein was expressed and purified. The purified AdoMetDC was inactivated by salicylaldehyde in a pseudo first- order kinetics. The secondorder rate constant for inactivation was 126 $M^{-1}min^{-1}$ with the slope of n=0.73, suggesting that inactivation is the result of the reaction of one lysine residue in the active site of AdoMetDC. Site-specific mutagenesis was performed on the AdoMetDC to introduce mutations in conserved $lysine^{81}$ residues. These were chosen by examination of the conserved sequence and proved to be involved in enzymatic activity by chemical modification. Changing $Lys^{81}$ to alanine showed an altered optimal pH. The substrate also provided protection against inactivation by salicylaldehyde. Considering these results, we suggest that the $lysine^{81}$ residue may be involved in catalytic activity.
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