• Proceedings of the Korean Society for Bioinformatics Conference
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• 2003.10a
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• pp.277-287
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• 2003

Acetolactate synthase (ALS, EC 4.1.3.18; also referred to as acetohydroxy acid synthase) catalyzes the first common step in the biosynthesis of valine, leucine, and isoleucine in microorganisms and plants. Recently X-ray structure of yeast ALS was available. Pair-wise alignment of yeast and tobacco ALS sequences revealed 63% sequence similarity. Using Deep View and automatic modeling on Swiss model server, we have generated reliable models of tobacco ALS based on yeast ALS template with a calculated pair-wise RMSD of 0.86 Angstrom. Functional roles of four residues located on the subunit interface (H142, El43, M350, and R376) were examined by site-directed mutagenesis. Seven mutants were generated and purified, of which three mutants (H142T, M350V, and R376F) were found to be inactivated under various assay conditions. The H142k mutant showed moderately altered kinetic properties. The E143A mutant increased 10-fold in K$_m$ value while other parameters remained unchanged. The M350C mutant was strongly resistant to three tested herbicides, while the R376k mutant can bind with herbicide carder at similar affinity to that of wild type enzyme, as determined by tryptophan quenching study. Except M350V mutant, all other mutants were ate to bind with cofactor FAD. Taken together, it is likely that residues H142 and E143 are located at the active site, while residues M350 and R376 are possibly located at the overlapping region of active site and herbicide binding site of the enzyme. Our data also allows us to hypothesize that the interaction between side chains of residues M350 and R376 are probably essential for the correct conformation of the active site. It remains to be elucidated that, whether the herbicide, upon binding with enzyme, inactivates the enzyme by causing change in the active site allosterically, which is unfavorable for catalytic activity.

• Earthquakes and Structures
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• v.10 no.5
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• pp.1233-1251
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• 2016

In this paper, it is aimed to determine the stochastic response of a suspension bridge subjected to spatially varying ground motions considering the geometric nonlinearity. Bosphorus Suspension Bridge built in Turkey and connects Europe to Asia in Istanbul is selected as a numerical example. The spatial variability of the ground motion is considered with the incoherence, wave-passage and site-response effects. The importance of site-response effect which arises from the difference in the local soil conditions at different support points of the structure is also investigated. At the end of the study, mean of the maximum and variance response values obtained from the spatially varying ground motions are compared with those of the specialised cases of the ground motion model. It is seen that each component of the spatially varying ground motion model has important effects on the dynamic behaviour of the bridge. The response values obtained from the general excitation case, which also includes the site-response effect causes larger response values than those of the homogeneous soil condition cases. The variance values calculated for the general excitation case are dominated by dynamic component at the deck and Asian side tower. The response values obtained for the site-response effect alone are larger than the response values obtained for the incoherence and wave-passage effects, separately. It can be concluded that suspension bridges are sensitive to the spatial variability of ground motion. Therefore, the incoherence, the wave-passage and especially the site-response effects should be considered in the stochastic analysis of this type of engineering structures.

• ALGAE
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• v.23 no.4
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• pp.257-268
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• 2008

Species composition, standing crop and dominant species of phytoplankton were investigated at 6 sites of Galgok stream and Incheon river which run into Gomso Bay, Jeollanam-do in April, August and November 2007. Total of 210 taxa were found which were classified as 6 classes, 12 orders, 25 families, 66 genera, 177 species, 27 varieties, 5 forms and 1 unidentified species. These river and stream had lower concentration of T-N and T-P compared to that of others; however, the site 2 of Galgok stream in summer was hypertrophic in T-P and also near hypertrophic in TN, and the site 1 in Incheon river during fall appeared to be hypertrophic in T-N, and the site 3 in summer showed near hypertrophic level in T-P. Determining the trophic status of the water quality based on chlorophyll a (chl-a), the site 3 of Galgok stream in spring and the site 3 of Incheon river in fall were oligotrophic; moreover, 6 sites including the site 2 and 3 of Galgok stream in summer were mesotrophic, and 9 sites including all sites of Galgok stream in fall turned out to be eutrophic. Particularly, the site 1 of Galgok stream in summer was hypertrophic, having 58.19 mg chl-a m$^{-3}$. There was a conspicuous difference between two values of standing crops based on chl-a and cell counting. This discrepancy may have occurred because of abundant cyanophytes and exclusion of picoplankton cells in cell counting. In the study, 5 cyanophytes, Synechocystis aquatilis, Microcystis aeruginosa, M. flos-aquae, Oscillatoria angustissima, O. limnetica and 2 diatoms, Thalassiosira bramaputrae and Navicula viridula var. rostellata were abundant. Based on the T-N, T-P values, standing crops and cell counting in Galgok stream and Incheon river were between mesotrophic and eutrophic conditions.

• Journal of Soil and Groundwater Environment
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• v.18 no.6
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• pp.38-47
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• 2013

This study was performed to identify the optimal operating conditions and to evaluate the xylene removal efficiency, applying in-situ soil flushing with the low concentrated solution of 'Tween 80' at the xylene contaminated site. The pilot scale test site ($5m{\times}5m{\times}3m$), was mainly composed of 'sandy loam', with the average hydraulic conductivity of $9.1{\times}10^{-4}cm\;s^{-1}$. The average xylene concentration of the site was 42.1 mg $kg^{-1}$, which was more than 2.5 times higher than Korea soil pollution warning limit (15 mg $kg^{-1}$). For the soil flushing, 7,800 L of 0.1~0.2% surfactant solution was injected into three injection wells at the average injection time of 9 hr $d^{-1}$ for 10 days, followed by the additional only groundwater injection of 6,000 L. The same amount of the effluent solution was extracted from three extraction wells. From the analysis for xylene concentration of all effluent at 3 extraction wells, total 166 g of xylene was removed by in-situ surfactant flushing. Even though the residual xylene concentrations of 7 soil sampling locations in the test site were different due to the soil heterogeneity, from the comparison of xylene concentration at 7 locations before/after the feasibility test, 53.9% of the initial xylene in the site was removed from three extraction wells (mainly Ext-N and Ext-M well). The results showed that the in-situ soil flushing by using low concentrated 'Tween 80' solution had a great potential to remediate the xylene contaminated site.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.42 no.4
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• pp.549-557
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• 2022

Due to harsh conditions of construction site, understanding of current feature of terrain and other infrastructures is critical issue for site managers. However, because of difficulties in acquiring the geographical information of the construction sites such as large sites and limited capability of construction workers, comprehensive site investigation of current feature of construction site is not an easy task for construction managers. To address these circumstances of construction sites, this study deduce difficulties and applicabilities of unmanned aerial vehicle in the area of construction site management. To confirm applicability of UAV in civil construction project, case study have been conducted on the road construction project. The result of case study proved that the developed system is one of promising technologies that has been studied in construction site management. To improve applicability of UAV for construction and process management information, law and technical issues will be an important area of future study.

• Journal of the Korean Association of Geographic Information Studies
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• v.13 no.4
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• pp.32-49
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• 2010

Site characterization on geological and geotechnical conditions was performed for evaluating the earthquake ground motions associated with seismic site effects at a small urbanized area, Hongseong, where structural damages were recorded by an earthquake of magnitude 5.0 on October 7, 1978. In the field, various geotechnical site investigations composed of borehole drillings and seismic tests for obtaining shear wave velocity profile were carried out at 16 sites. Based on the geotechnical data from site investigation and additional collection in and near Hongseong, an expert system on geotechnical information was implemented with the spatial framework of GIS. For practical application of the GIS-based geotechnical information system to assess the earthquake motions in a small urban area, spatial seismic zoning maps on geotechnical parameters, such as the bedrock depth and the site period ($T_G$), were created over the entire administrative district of Hongseong town, and the spatial distributions of seismic vulnerability potentials were intuitively examined. Spatial zonation was also performed to determine site coefficients for seismic design by adopting a site classification system based on $T_G$. A case study of seismic zonation in the Hongseong area verified that the GIS-based site investigation was very useful for regional prediction of earthquake ground motions in a small urbanized inland area.

• Journal of Korean Society of Environmental Engineers
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• v.34 no.4
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• pp.232-238
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• 2012

In this study, the noise mapping simulation is executed to design an effective barrier reducing noise levels of a school site. The geographical features of the ambient site and the school buildings are modelled in detail in order to consider sound propagation, deflection, and absorption phenomena etc. The main sound source, sound power level of expressway, is estimated on the basis of measured noise levels at several points of the site. The noise mapping simulation is performed by using ENPro, environmental noise prediction program based on ISO 9613 to analysis the effectiveness of noise barrier. Consequently, the noise barrier is designed to meet an environmental noise standard and satisfy low cost and safety conditions.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.17 no.5
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• pp.192-195
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• 2007

Structural and electronic properties of bulk GaN with nitrogen vacancy($V_N$), oxygen substitution on nitrogen site($O_N$), and complex of nitrogen vacancy and oxygen substitution on nitrogen site($V_N-O_N$) were investigated using the first principle calculations. It was found that stability of defect formation is dependent on the epilayer growth conditions. The complex of $V_N-O_N$ is energetically the most favorable state in a condition of Ga-rich, however, oxygen substitution in nitrogen site is the most favorable state in N-rich condition. The electronic property of complex with negative charge states at $\Gamma$ point was changed from semiconductor to metal. On the contrary, the properties of nitrogen vacancy except for neutral charge state have shown the semiconductor characteristics at $\Gamma$ point. In the oxygen substitution on nitrogen site, the energy differences between conduction band minimum and Fermi level were smaller than that of defect-free GaN.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2003.11a
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• pp.276-282
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• 2003

The geoscientific study on geological disposal for radioactive wastes has established the stepwise site characterization program, methods and investigation technology. However the intrinsic properties of geological material such as heterogeneity and scale dependent properties make difficulty on satisfactory understanding of geological conditions. To avoid unnecessary time delay and unexpected extra-cost for site investigation, the accurate and complete site investigation program should be established in a stepwise manner and the QC programs for investigation methods and procedures. Moreover, the technical requirements and preferences for a repository should be distinguished and be assessed at the end of each investigation step.

• Journal of Environmental Impact Assessment
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• v.22 no.1
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• pp.79-87
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• 2013

The purpose of this study is to establish PM-10 management manual for developing large scale sites by assessing the status of PM-10 reduction at ongoing large scale development sites. After analyzing the meteorological conditions and air quality characteristics of Sihwa MTV development site, ISCST3 (Industrial Source Complex Short Term Model 3) was implemented to predict PM-10 generation. The outcomes of ISCST3 modelling were utilized for verification of site survey data. As a result of applying air pollution modeling, the diffusion rate of PM-10 decreases according as the wind speed decreases. And the emission rate of PM-10 increase is linear to the concentration of PM-10. The reduction target of PM-10 can be derived quantitatively from the difference between the forecasted emission rate and the permissible emission limit of PM-10. The assessment of PM-10 characteristics which is deduced from ISCST3 and site survey can be practically applied to accomplish environmentally acceptable air quality manual for large scale development sites.