• Bulletin of the Korean Chemical Society
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• 제30권4호
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• pp.913-916
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• 2009

Femtosecond vibrational spectroscopy was used to probe the dynamics of CO rebinding to hemoglobin (Hb), denatured by 4.0 M GdnHCl in $D_2O# at 283 K, after photolysis of HbCO. The stretching mode of $^{13}CO$ bound to the denatured $Hb^{13}CO$ showed a single band centered at 1922 $cm^{-1}$, indistinguishable from that of denatured $Mb^{13}CO$. Geminate rebinding of CO to the denatured Hb was accelerated more than 1000 times, suggesting that the native structure of the Hb is required to suppress efficient geminate rebinding of CO, as is the case in Mb. The geminate yield and rate for CO rebinding are almost the same in both the denatured Hb and Mb. Similarity in the equilibrium spectrum and rebinding dynamics of CO indicates that the state of the denatured Hb is very similar to that of the denatured Mb. In the denatured Hb, quaternary contact of the protein is likely severed, with the denatured protein existing as an independent subunit much like Mb.

• KOREAN JOURNAL OF CROP SCIENCE
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• 제42권2호
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• pp.134-140
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• 1997

The monoclonal antibody against a novel protein, which was induced in the roots of rice seedlings treated with NaCl, was produced. Oryzea sativa L. cv. Annapruna grains were sown on clean sands and grown for 10days and then the seedlings were soaked in 50mM NaCl aqueous solution. In 48hrs of the NaCl treatment, the roots were collected and homogenated with liquid nitrogen and extraction buffer. The homogenate was centrifuged and to the supernatant 75％ ammonium sulfate was added to pre-cipitate proteins. From these proteins a novel protein was purified through DEAE-ion chromatography and FPLC(Phenyl column). This protein appeared as a single band in the native electrophoresis. Using this protein as antigen, monoclonal antibody was produced. Five cell lines that secreted antibodies specifically bound to this protein were constructed.

• Journal of Microbiology and Biotechnology
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• 제22권2호
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• pp.226-229
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• 2012

Bacterial hemoglobin from Vitreoscilla (VHb) is recognized as a good fusion protein for the soluble expression of foreign protein. In this study, we generated a monoclonal antibody (MAb) against VHb for its detection. For the rapid screening of MAb, a protein chip technology based on the Alexa-488 (A488) dye labeling method was introduced. In order to fabricate the chip, the VHb protein was chemically coupled to the chip surface and then the culture supernatants of 84 hybridoma cell lines were spotted onto the VHb chip. The bound MAbs were measured by A488-modified anti-mouse IgG. A single spot (MAb A10) exhibited significantly high signal intensity. The immunoblot analysis evidenced that the MAb A10 can detect VHb-fused proteins with high specificity.

• Proceedings of the Korean Vacuum Society Conference
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• 한국진공학회 2009년도 제38회 동계학술대회 초록집
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• pp.69-69
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• 2010

The research is the structural and optical characteristics of the Cadmium Sulfide(CdS) film, nanowires and nanobelts grown on the $Al_2O_3$ substrate using the vapor phase epitaxy method. The field-emission scanning electron microscopy(FE-SEM) were used to identify the shape of the surface of the nanostructures and x-ray diffraction(XRD) and transmission electron microscopy (TEM) were used to evaluate the structural characterisitcs. As a result, the XRD was confirmed the CdS peak and the substrate peak and TEM showed single crystals with wurtzite hexagonal structure on the nanostructures. As for the optical characteristic of the nanostructures, photoluminescence(PL) and micro-raman spectrum were measured. The PL measurements confirmed the emission peak related bound exciton to neutral donor($D^0X$) peak and free exciton(FX) peak. The micro-raman spectrum showed that the peak of the nanostructures were similar to the pure crystalline CdS peak and each peak were overtone of LO phonon of the hexagonal CdS of the longitudinal optical(LO) phonon mode. Therefore, it is confirmed that the CdS nanostructures grown in this research have superior crystallinity.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제12권5호
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• pp.2124-2141
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• 2018

Recent studies have revealed the efficacy of incorporating delayed channel state information at transmit side (CSIT) in transmission scheme design. This paper focuses on transmit precoder design to maximize the ergodic sum-rate in a two-user Multiple-Input Single-Output (MISO) system with delayed and statistical CSIT. A new transmit strategy which precodes signals in all transmit slots is proposed in this paper, denoted as all time-slots precoding Alternative MAT (AAMAT). There is a common procedure in conventional delayed-CSIT based schemes, which is retransmitting the overheard interferences. Since the retransmitting signal is intended to both users, all previous schemes tend to use only one antenna. We however figure out an improvement in spectral efficiency could be realized if all antennas can be utilized. In this paper, we detail the design of the procoder which enabling all antennas and also we compute a lower bound of the ergodic sum-rate in an ideal condition. In addition, simulation results demonstrate the superiority of our proposed scheme.

• Bulletin of the Society of Naval Architects of Korea
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• 제19권1호
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• pp.3-14
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• 1982

In this paper, the sway added mass of a rectangular cylinder in a restricted water is considered by applying Hamilton's principle as the frequency tends to zero. The present method is an extension of Isshiki's method proposed in 1978. In the present method, it is assumed that the fluid velocity distribution in each subdomain of the fluid can be represented by higher order polynomials while Isshiki assumed linear velocity distribution. The fluid flow is assumed as a rotational motion in the present analysis. However, the results obtained from the present method show good agreement with Bai's numerical results for the case of large clearances between a canal wall and a cylinder. From Kelvin's minimum energy theorem, we can see that the value of sway added mass obtained from the present method approaches the upper bound. The approximate formula obtained in the present study takes a simple form which consists of the dimensions of the canal and the cylinder. The present formulae are derived for the cases of a rectangular cylinder swaying at the center of a narrow or wide canal relative to a cylinder, at off-center location in a canal, and in the restricted water with a single wall. From the results of numerical calculation, it is concluded that the sway added mass in restricted waters is more affected by water depth than clearance between a wall and a cylinder.

• Geomechanics and Engineering
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• 제32권6호
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• pp.639-652
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• 2023

Stepped earth slopes incorporated with anti-slide piles are widely utilized in landslide disaster preventions. Explicit consideration of the three-dimensional (3D) effect in the slope design warrants producing more realistic solutions. A 3D limit analysis of the stability of pile stabilized stepped slopes is performed in light of the kinematic limit analysis theorem. The influences of seismic excitation and surcharge load are both considered from a kinematic perspective. The upper bound solution to the factor of safety is optimized and compared with published solutions, demonstrating the capability and applicability of the proposed method. Comparative studies are performed with respect to the roles of 3D effect, pile location, pile spacing, seismic and surcharge loads in the safety assessments of stepped slopes. The results demonstrate that the stability of pile reinforced stepped slopes differ with that of single stage slopes dramatically. The optimum pile location lies in the upper portion of the slope around L_x/L = 0.9, but may also lies in the shoulder of the bench. The pile reinforcement reaches 10% universally for a looser pile spacing D_c/d_p = 5.0, and approaches 70% when the pile spacing reaches D_c/d_p = 2.0.

• Korean Chemical Engineering Research
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• 제61권2호
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• pp.273-277
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• 2023

The biosorption of methylene blue (MB) from aqueous solution was investigated using dried Rhodotorula glutinis as the biosorbent. The effects of pH, initial dye concentration, biosorbent dosage, and kinetic studies were determined to obtain valuable information for biosorption. Results indicated that most of the adsorbed MB bound within 30 minutes of contact and the MB adsorption capacity increased from 21.1 to 101.8 mg/g with the initial MB concentration increased from 50 to 300 mg/L. Additionally, the MB adsorption capacity gradually increased from pH 4.0 to 9.0, reaching its peak at an initial pH of 9.0. As the biomass load was increased from 0.25 to 4.0 g/L, the MB removal efficiency increased from 14.1 to 84.5%. The Langmuir model provided the best fit throughout the concentration range, and the maximum adsorption capacity (q_max) and Langmuir constant (b) were determined to be 135.14 mg/g and 0.026 l/mg, respectively. Furthermore, the biosorbent process of R. glutinis was found to follow pseudo-second-order kinetics and the calculated q_eq,cal value showed good agreement with the experimental q_eq value. Overall, the biosorption of MB by R. glutinis can be characterized as a monolayer, single site type phenomenon, and the rate-limiting step was determined to be the chemical reaction between the adsorbent and the adsorbate.

• Biodesign
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• 제5권3호
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• pp.122-125
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• 2017

Receptor for advanced glycation end products (RAGE) is one of the single transmembrane domain containing receptors and causes various inflammatory diseases including diabetes and atherosclerosis. RAGE extracellular domain has three consecutive IgG-like domains (V-C₁-C₂ domain) which interact with various soluble ligands including heparan sulfate or HMGB1. Studies have shown that each ligand induces different oligomeric forms of RAGE which results in a ligand-specific signal transduction. The structure of mouse RAGE bound to heparan sulfate has been previously determined but the electron density map of heparan sulfate was too ambiguous that the exact position of heparin sulfate could not be defined. Furthermore, the complex structure of human RAGE and heparin sulfate still remains elusive. Therefore, to determine the structure, human RAGE was overexpressed using bacterial expression system and crystallized using the sitting drop method in the condition of 0.1 M sodium acetate trihydrate pH 4.6, 8 % (w/v) polyethylene glycol 4,000 at 290 K. The crystal diffracted to 3.6 Å resolution and the space group is C121 with unit cell parameters a= 206.04 Å, b= 68.64 Å, c= 98.73 Å, α= 90.00°, β= 90.62°, γ= 90.00°.

• Journal of English Language & Literature
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• 제58권6호
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• pp.1103-1117
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• 2012

This paper focuses on eighteenth-century English pastor, poet, and hymnist, Isaac Watts (1674-1748), a significant yet neglected nonconformist dissenter, who defines a public religion and transforms poetry as a new literary political genre. During England's post-Revolutionary religio-political turmoil, Watts's poem, "The Hurry of the Spirits, in a Fever and Nervous Disorders" (1734), deliberately engages in a methodical refusal to settle upon a single system of images or terms for describing or referring to the speaker's identity or situation. Watts's, literal and metaphoric, refusal to identify with one religio-political approach to nonconformist dissent has been the very point of criticism that not only undermines the poet's monumental work on hymns but also the lasting impact that the poet had upon England's national consciousness. This study, therefore, questions why the poet refuses to choose one ideal path in his pursuit for religious freedom and, further, analyzes how the hymn writer defends his demotic aesthetics. This paper investigates Watts's comprehensive and detailed formulation of what a secularized "social religion" should entail and, further, explores its beneficial role in the pursuit for society's peace. In contrast to Milton's apocalyptic vengeance, Watts's nonconformist goal seeks to balance and locate authority in the individual with the ancient ideal of a "sacred order" that is represented in "The Hurry of the Spirits" through the means of poetic imagination.