• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.4
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• pp.212-215
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• 2002

Tyrosinase transcript In the blood Is known as the marker of malignant melanoma and it has been often determined by using reverse transcription-polymerase chain reaction (RT-PCA) . However, after the PCR process, the quantification of amplified CDMA by the gel electrophoresis is not reliable and time-consuming. for this reason, we tried to quantify the PCR product using a cuvette-type biosensor, where the oligonucleotide probe was immobilized on the cuvette surface and the single strand CDMA, the denatured PCH product, was then hybridized onto the immobilized probe to give a response signal. The response was Immediate and takes 15 min to obtain a stable signal. The biosensor was much more sensitive comparing to the gel electrophoresis method. The quantification of PCR product using a cuvette-type biosensor was feasible and rapid.

• BMB Reports
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• v.39 no.2
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• pp.140-144
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• 2006

A deoxyriboendonuclease has been purified to near homogeneity from a fast growing mycobacterium species, M. smegmatis and characterized to some extent. The size of enzyme is about 43 kDa as determined by a denaturing gel analysis. It shows optimum activity at $32^{\circ}C$ in Tris-HCl buffer (pH 7.2) containing 2.5 mM of $MgCl_2$. Both EDTA and $K^+$ but not $Na^+$ inhibit its activity. Evidences show that the enzyme is not a restriction endonuclease but catalyzes the endonucleolytic cleavage of both the double- as well as the single-strand DNA non-specifically. It has been shown that the cleavage by this enzyme generates DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, respectively. Analysis reveals that no endonuclease having size and property identical to our deoxyriboendonuclease had been purified from M. smegmatis before. The property of our enzymes closely matches with the deoxyriboendonucleases purified from diverse sources including bacteria.

• Archives of Pharmacal Research
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• v.29 no.3
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• pp.213-217
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• 2006

The present study was undertaken to test the hypothesis that dietary antioxidants protect DNA damage induced by peroxynitrite, a potent physiological inorganic toxin. The present study showed that dietary antioxidants such as (-)-epigallocatechin gallate, quercerin, rutin, resveratrol, and ursolic acid inhibit single strand breaks in supercoiled plasmid DNA induced by 3-morpholinosydnomine N-ethylcarbamide (SIN-1), a generator of peroxynitrite through the reaction between nitric oxide and superoxide anion. The formation of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) in calf thymus DNA by SIN-1 was also inhibited by dietary antioxidants. When U937 cells were incubated with 1 mM SIN-1 bolus, a significant increase of 8-OH-dG level was observed. However, oxidative DNA damage was significantly lower in the cells pre-treated with dietary antioxidants when cells were exposed to SIN-1.

• BMB Reports
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• v.36 no.4
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• pp.399-402
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• 2003

Early detection of bladder cancer is particularly important since it dramatically affects the survival rates. However, neither urinary cytology nor tumor markers that are currently used are sensitive enough for the early detection of bladder cancer or recurrent disease. The ras genes are frequently mutated in cancer. In this study, we investigated the diagnostic potential of ras mutation analysis in urinary sediments of patients with bladder cancer using a single-strand conformation polymorphism analysis and polymerase chain reaction. Mutation in codon 12 of the H-ras gene was observed in 39% of the patients. Our results indicate that this approach may significantly improve diagnostic sensitivity in detecting bladder tumors.

• Journal of Microbiology
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• v.34 no.3
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• pp.236-240
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• 1996

Rifampin is the most powerful drug for treating leprosy and tuberculosis today. It inhibits initiation and elongation of RNA transcription by binding to $\beta$-subunit of RNA polymerase, leading to kill mycobacteria. We isolated one variant strain of Mycobacterium leprae from 24 Korean leprosy patients who are less susceptible to rifampin or have suffered from relapse by polymerase chain reaction and single strand conformation polymorphism (PCR-SSCP) of the rpoB gene. Direct sequencing of the rpoB region of M. leprae variant revealed missense mutations which altered the amino acids sequenceof RpoB to Ser-464, Arg-465, Arg-467 and Ala-468. This is the first finding on rpoB gene mutation of M. leprae from Korean patients ; moreover the mutant type was found to be different from the previously reported cases in other countries.

• Proceedings of the Optical Society of Korea Conference
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• 2003.02a
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• pp.312-313
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• 2003

최근 인터넷 트래픽의 증가와 음성, 데이터, 동영상과 같은 다양한 멀티미디어 서비스의 영향으로 가입자망의 고속화가 요구되고 있다. 인터넷 트래픽을 효율적으로 가입자에게 제공하기 위해서는 광을 이용한 통신이 이루어져야 한다. 이를 위하여 다양한 기술들이 제시되고 있으며, 경제적인 측면과 구성의 용이성을 고려한 광 가입자망인 PON(Passive Optical Network)에 대한 관심이 고조되고 있다. 본 논문에서는 파장 분할 다중화 방식으로 단심 상하향 동일파장을 사용하여 소요되는 광섬유의 비용을 반으로 줄이고, 채널당 전송용량을 두 배로 높일 수 있는 매트로 이더넷용 단심 양방향 버스형 WDM-EPON 시스템에 대한 연구를 하고자 한다. (중략)

• Journal of Microbiology and Biotechnology
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• v.25 no.4
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• pp.492-495
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• 2015

This study provides evidence that RecG regulates the expression of the OxyR-independent gene mexA in Pseudomonas aeruginosa PAO1. A reduction in mexA expression was observed in the absence of RecG, but not OxyR, by northern blot and quantitative real-time PCR analyses. The canonical palindromic RecG binding sequence was present upstream of the mexA promoter, and bound purified RecG and single strand-binding protein. These data reveal a novel mechanism of OxyR-independent gene transcription by RecG.

• Proceedings of the KIEE Conference
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• 2000.07c
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• pp.2257-2259
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• 2000

This paper reports on the design, fabrication and driving experiment of micro mirror array(MMA) for lithography process to apply to biochip fabrication Photolithography technology is applied to activate specific area on the surface of modified glass surface, DNA monomers are bound on the activated area of the glass surface. After repeat of DNA monomer synthesizing process, DNA single strand probes could be solid-synthesized on the glass substrate. Without using photomask, photolithography process is tried using micro mirror array(MMA). Photomask or mask alignment is not required in maskless photolithography process using micro mirror array.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2003.03a
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• pp.377-384
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• 2003

Recently, there are much concerns about new and innovative transverse materials which could be used instead of conventional transverse steel in reinforced concrete bridge piers. FRP materials could be substituted for conventional transverse steel because of their sufficient strength, light weight, easy fabrication, and useful applicability to any shapes of pier sections, such as rectangular or circular sections. The objective of this research is to evaluate the seismic performance of reinforced concrete bridge pier specimens with FRP transverse reinforcement by means of the Quasi-Static test. In the first task, test columns were made using FRP rope, but these specimens appeared to fail at low displacement ductility levels due to insufficient confinement of strand extension itself. Therefore, the second task was to evaluate the seismic performance of test specimens transversely confined with FRP band. Although FRP banded specimens showed lower seismic performance than the specimen with spiral reinforcing steel, it satisfied with the response modification factor, 3, required for the single column of Korea bridge roadway design code. It was concluded that FRP band could be efficiently substituted for conventional reinforcing steel.

• Bulletin of the Korean Chemical Society
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• v.19 no.4
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• pp.444-449
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• 1998

The kinetics of the formation of triplex $poly(dA){\cdot}[poly(dT)]_2$ from poly(dA)·poly(dT) and poly(dT) is examined by various optical spectroscopic methods, including absorption, circular and linear dichroism (LD) spectroscopy. In the pseudo first order condition, where the poly(dT) concentration is kept lower than that of duplex, the association of the poly(dT) is enhanced by the presence of ethidium; the rate constant is proportional to the amount of ethidium in the mixture. When the concentration of the duplex and the single strand is the same, a spectral change is explained by double exponential curves, indicating that at least two steps are involved, the fast association and slow rearrangement steps. In contrast to the pseudo first order kinetics, the association step in an equimolar condition is not affected by the presence of ethidium. In the rearrangement step, the magnitude of LD decreases with an increase in ethidium concentration, suggesting that the bending of polynucleotide around the intercalation site occurs in the rearrangement step.