• Journal of Korea Association of Health Promotion
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• v.4 no.1
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• pp.68-74
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• 2006

For identification of four sibling species of the Anopheles hyrcanus complex found in Korea, the 5.8 rDNA-ITS2-28S rDNA region of each species was sequenced and the species-specific primers wee designed The amplified PCR products obtained from each species were analyzed by agarose gel electrophoresis. The result showed a single species- specific band, I.e. 559bp, 432bp, 322bp and 192bp for An. sinensis, An. sp., An. lesteri and An. pullus, respectively. In conclusion, the species-specific PCR primers designed from ITS2 variable regions functioned successfully and specifically, and can be applied as a useful tool for identifying species of the Anopheles hyrcanus complex found in Korea.

• Parasites, Hosts and Diseases
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• v.25 no.2
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• pp.207-209
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• 1987

A systematic mosquito taxonomic study in Korea was established by Lee in 1971. Since then, many species have been recorded as new to Korea. However, much of this taxonomic information has been distributed in a number of scientific publications. This fork attempts to combine this information into a single document, as well as to synthesize revised taxonomic synonyms of species cccurring in Korea. Four species, Culex vora.x, Culex kangi, Aedes kobayashii and Culiseta kanayamensis listed in our previous paper, are synonyms of Culex kalifaxii, Cutex jackseni, Aedes alehtorowi and Culiseta bergrothi, respectively. Culex annulus is a misidentified species of Culex pseudovishnui. The subspecies Cutes pipiens quinquefassiatus and subgenus Manscnia (Ccquillettidia) were treated as full species and genus respectively in a catalog of the mosquitoes of the world. However, not all current authors agree to those specific and generic treatment. Based on available publications the total number of mosquito species recorded in Korea is now 52 representing 9 genera. Forty-five of these 52 are confirmed species with preserved specimen representation. As collection of specimen continues, there is a possibility that representative of 7 unconfirmed species will be found. 1 hope this paper will provide useful information for entomology workers who are interested in mosquito taxonomy in Korea.

• Journal of Plant Biotechnology
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• v.45 no.2
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• pp.102-109
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• 2018

Thistle is a perennial plant that is widely used for medicinal purposes. Information on the genetic diversity of thistle populations are great important for their conservation and germ plasmic utilization. Although thistle is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish them from other similar species from different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from the nuclear ribosomal DNA internal transcribed spacer (ITS) regions of genomic sequences to identify distinct Korean-specific thistle species via an amplification refractory mutation system (ARMS)-PCR and high resolution melting (HRM) curve analyses. We performed molecular authentication of four different kinds of thistle species from different regions using DNA sequences in the ITS intergenic region. We also developed a quantitative PCR assay using species-specific ITS primers, which allowed us to estimate the ratio of Korean-specific thistle species using varying ratios of mixed genomic DNA templates from the two species. The SNP markers developed in this study are useful for rapidly identifying specific thistle species from different countries.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.41 no.4
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• pp.296-305
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• 2005

Acoustic target strength (TS) of 12 commercially important fish species caught in the Korean waters had been investigated and their results were presented. Laboratory measurements of target strength on 12 dominant fish species were carried out at a frequencies of 75 kHz by single beam method under the controlled condition of the water tank with the 241 samples of dead and live fishes. The target strength pattern on individual fish of each species was measured as a function of tilt angle, ranging from $-45^{\circ}$ (head down aspect) to $45^{\circ}$ (head up aspect) in $0.2^{\circ}$ intervals, and the averaged target strength was estimated by assuming the tilt angle distribution as N ($-5.0^{\circ}$, $^15.0{\circ}$). The 75 to fish length relationship for each species was independently derived by a least - squares fitting procedure. Also, a linear regression analysis for all species was performed to reduce the data to a set of empirical equations showing the variation of target strength to fish length and fish species. An empirical model for fish target strength(TS, dB) averaged over the dorsal aspect of 158 fishes of 7 species and which spans the fish length(L, m) to wavelength(${\lambda}$, m) ratio between 6.2 and 21.3 was derived: TS: 27.03 Log(L)-7.7Log(${\kanbda}$)-17.21, ($r^2$=0.59).

• Korean Journal of Plant Taxonomy
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• v.53 no.1
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• pp.47-53
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• 2023

We have determined the complete chloroplast genome of Erigeron Canadensis isolated in Korea. The circular chloroplast genome of E. canadensis is 152,767 bp long and has four subregions: 84,317 bp of large single-copy and 18,446 bp of small single-copy regions are separated by 25,004 bp of inverted repeat regions including 133 genes (88 protein-coding genes, eight rRNAs, and 37 tRNAs). The chloroplast genome isolated in Korea differs from the Chinese isolate by 103 single-nucleotide polymorphisms (SNPs) and 47 insertions and deletion (INDEL) regions, suggesting different invasion sources of E. canadensis in Korea and China. A nucleotide diversity analysis revealed that the trend of the nucleotide diversity of E. canadensis followed that of 11 Erigeron chloroplasts, except for three peaks. The phylogenetic tree showed that our E. canadensis chloroplast is clustered with E. canadensis reported from China. Erigeron canadensis can be a good target when attempting to understand genetic diversity of invasive species.

• 한국연소학회:학술대회논문집
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• 2015.12a
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• pp.301-304
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• 2015

With the experiment observation of single particle combustion, this model is built for the numerical analysis of the process. It's about the single coal particle combustion process under different conditions with reasonable assumptions. The model can express the mass, radius, density, temperature changing with different particle sizes, oxygen concentration and gas temperature. It also includes the flame sizes change in different condition and the diffusion of each species. The result shows the characters of the combustion.

• Korean Journal of Environmental Biology
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• v.33 no.2
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• pp.153-159
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• 2015

The oligochaete worms in the genus Nais are common to freshwater habitats. These worms have a highly-developed prostomium and pigmented eye spots. All species have hair chaetae, bifid or single pointed needle chaetae on the dorsal side and ventral chaetae beginning in II with bifid crotchets. Most species of Nais genus live in areas where bottom is covered by sand, gravel or organic matter in aquatic plants. Currently, 26 species of Nais have been recorded worldwide. From the recent faunal studies, 3 species of Nais have been newly reported in Korea. Here, we newly report 6 species of Nais with diagnosis, illustrations and identification keys to Korean Nais species: N. behningi, N. pseudobtusa, N. simplex, N. bretscheri, N. stolci and N. elinguis.

• Korean Journal of Plant Taxonomy
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• v.51 no.4
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• pp.345-352
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• 2021

Diarthron linifolium Turcz. is an annual herb usually found in sandy soil or limestone areas. Plants in the genus Diarthron are known to have toxic chemicals that may, however, be potentially useful as an anticancer treatment. Diarthron linifolium is a unique species among the species of the genus distributed in Korea. Here, we determine the genetic variation of D. linifolium collected in Korea with a full chloroplast genome and investigate its evolutionary status by means of a phylogenetic analysis. The chloroplast genome of Korean D. linifolium has a total length of 172,644 bp with four subregions; 86,158 bp of large single copy and 2,858 bp of small single copy (SSC) regions are separated by 41,814 bp of inverted repeat (IR) regions. We found that the SSC region of D. linifolium is considerably short but that IRs are relatively long in comparison with other chloroplast genomes. Various simple sequence repeats were identified, and our nucleotide diversity analysis suggested potential marker regions near ndhF. The phylogenetic analysis indicated that D. linifolium from Korea is a sister to the group of Daphne species.

• Journal of Species Research
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• v.11 no.2
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• pp.98-101
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• 2022

The genus Tripylina Brzeski, 1963 is a group of terrestrial nematodes that inhabit soil and semi-wet biomes, most of which have been reported from Europe, Asia, America, Africa, and New Zealand. Tripylina stramenti (Yeates, 1971) Tsalolikhin, 1983, belonging to the family Trischistomatidae Andrassy, 2007, is newly discovered from Korea. The specimens were collected from the edge of the valley of Ulleung-gun, Gyeongsangbuk-do, South Korea. Tripylina stramenti described herein shows typical morphological characters of the genus Tripylina, including six and four setae in a single whorl, present dorsal tooth and subventral teeth, prodelphic reproductive system of female, absence post-uterine sac, narrow sickle-shaped spicule of male and generally S-shaped tail. The specimens differ in their body width (from the New Zealand populations), outer labial setae length, cuticles thickness(from the West African populations), and nerve ring position (from Indian populations). This species is distinguished from other Tripylina species by its relatively large body, two anterior and subventral teeth, and single cervical seta. In this study, morphological characteristics and morphometric information of the Korean T. stramenti isolate are described, illustrated, and compared with the same species from other geographic origins.

• Journal of Species Research
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• v.12 no.1
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• pp.55-67
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• 2023

Two new records of marine nematodes, belonging to the family Ironidae de Man, 1876, are reported based on the specimens collected from intertidal sediments from the west coast of Korea. Conilia sinensis is characterized by a relatively long body length, a well-developed buccal cavity with three claw-like teeth, the presence of a single spicule with transverse striations, the presence of rib-shaped telamons, and single precloacal supplement. Pheronous donghaiensis is distinguished from other species of the genus by a well-developed buccal cavity with four claw-like solid teeth and minute denticles, relatively short and thick spicules with the central septum, the presence of 8-9 curved tubular-shaped precloacal supplements, and a sharp pointed tail with three small tubular supplements arranged into two rows in males. In this report, we provide a taxonomic description and illustrations of two unrecorded species of the family Ironidae by differential interference contrast microscopy. This is the first taxonomic report on the species of the family Ironidae from Korea.