Journal of Korea Association of Health Promotion (한국건강관리협회지)

KOREA ASSOCIATION OF HEALTH PROMOTION (한국건강관리협회)
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Species identification of the Anopheles kyrcanus complex found in Korea using PCR

PCR을 이용한 우리나라에서 발견되는 얼룩날개모기속 모기의 종 동정

  • Published : 2006.06.01
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Abstract

For identification of four sibling species of the Anopheles hyrcanus complex found in Korea, the 5.8 rDNA-ITS2-28S rDNA region of each species was sequenced and the species-specific primers wee designed The amplified PCR products obtained from each species were analyzed by agarose gel electrophoresis. The result showed a single species- specific band, I.e. 559bp, 432bp, 322bp and 192bp for An. sinensis, An. sp., An. lesteri and An. pullus, respectively. In conclusion, the species-specific PCR primers designed from ITS2 variable regions functioned successfully and specifically, and can be applied as a useful tool for identifying species of the Anopheles hyrcanus complex found in Korea.

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