• The Plant Pathology Journal
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• v.26 no.4
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• pp.409-416
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• 2010

Fusarium species, a large group of plant pathogens, potentially pose quarantine concerns worldwide. Here, we focus on the development of a method for detecting four Fusarium species in quarantined plants in Korea: F. solani f. sp. cucurbitae, F. stilboides, F. redolens, and F. semitectum var. majus. Species-specific primers were designed from the nucleotide sequences of either the translation elongation factor-1 alpha (TEF1) gene or RNA polymerase II subunit (RPB2) gene. Two different primer sets derived from TEF1, all specific to F. solani f. sp. cucurbitae, were able to differentiate the two races (1 and 2) of this species. A set of nested primers for each race was designed to confirm the PCR results. Similarly, two primer sets derived from RPB2 successfully amplified specific fragments from five F. stilboides isolates grouped within a single phylogenetic clade. A specific TEF1 primer set amplified a DNA fragment from only four of the 12 F. redolens strains examined, which were grouped within a single phylogenetic clade. All of the F. semitectum var. majus isolates could be specifically detected with a single RPB2 primer set. The specificity of the primer sets developed here was confirmed using a total of 130 Fusarium isolates.

• The Plant Pathology Journal
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• v.15 no.3
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• pp.131-136
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• 1999

In fungi known as ascomycetes, ability to mate is controlled by a single mating type (MAT) locus with two dissimilar sequences called idiomorphs carrying genes encoding transcription factors that are unrelated to each other. Fungi requiring strains with different MAT genes to complete the sexual process are heterothallic (self-sterile); species in which as single strain is able to undergo sexual reproduction are homothallic (self-fertile). Previous analysis of sequences from several heterothallic and homothallic species of the ascomycete genus Cochliobolus showed that homothallics evolve from heterothallics and that each known Cochliobolus homothallic species arose independently, from a different heterothallic ancestral species. Here we report detailed comparative analyses of MAT sequences ad their flanking regions, and show that: (1) The level of MAT gene similarity is not correlated with reproductive life style; (2) MAT proteins from all Cochliobolus species are conserved within the transcription factor signature sequences; they are not conserved in the carboxy terminal half of MAT-1, or third of MAT-2, except in those from very closely related species; (3) A gene (ORF1) of unknown function, consistently found on the MAT flank, is more conserved than are the MAT genes themselves; (4) The intergenic sequences diverge sharply among species.

• Aerospace Engineering and Technology
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• v.11 no.1
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• pp.126-134
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• 2012

The Single Species, Unreacted Flow Model which is effectively applicable on the computational analysis of rocket exhaust flow is introduced in this paper. The basic concept of this model had been originated from chemically frozen analysis of hot air but it was complemented by compensating molecular weight and specific heat which was obtained CEA code analysis of exhaust plume. Comparing single species, unreacted model with the finite chemistry model, unreacted model can reduce calculation time to 1/5 while it makes similar simulation results.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.414-418
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• 2003

Through the PVA degrading test, 8 species of microbes were finally isolated, and two species among them were identified. To search PVA degrading rate by using 8 species of microbes, single species of microbes and combination of each species were tested. As a result, single species of microbes showed 96% of PVA degrading rate, and the similar result was obtained by using combination of two species. And 78% of PVA degrading rate was obtained by using enzyme which was secreted from good PVA degrading microbes. As a result of identification, this good PVA degrading microbes were Paenibacillus sp. and Microbacterium barkeri.

• Journal of Periodontal and Implant Science
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• v.48 no.1
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• pp.12-21
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• 2018

Purpose: The goal of this study was to develop and validate a standardized in vitro pathogenic biofilm attached onto saliva-coated surfaces. Methods: Fusobacterium nucleatum (F. nucleatum) and Porphyromonas gingivalis (P. gingivalis) strains were grown under anaerobic conditions as single species and in dual-species cultures. Initially, the bacterial biomass was evaluated at 24 and 48 hours to determine the optimal timing for the adhesion phase onto saliva-coated polystyrene surfaces. Thereafter, biofilm development was assessed over time by crystal violet staining and scanning electron microscopy. Results: The data showed no significant difference in the overall biomass after 48 hours for P. gingivalis in single- and dual-species conditions. After adhesion, P. gingivalis in single- and dual-species biofilms accumulated a substantially higher biomass after 7 days of incubation than after 3 days, but no significant difference was found between 5 and 7 days. Although the biomass of the F. nucleatum biofilm was higher at 3 days, no difference was found at 3, 5, or 7 days of incubation. Conclusions: Polystyrene substrates from well plates work as a standard surface and provide reproducible results for in vitro biofilm models. Our biofilm model could serve as a reference point for studies investigating biofilms on different surfaces.

• Journal of Plant Biology
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• v.28 no.1
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• pp.29-44
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• 1985

This study was carried out to investigate the types and frequency of abnormal stomata in Crassulaceae. We observed the surface of the leaves on twenty four species involved five varieties belonging to eight genera of Crassulaceae. Nineteen types of abnormal stomata were found in the families. Among them three types such as the single guard cell, the aborted stomata, and the single-aborted guard cell were highly observed from nineteen to twenty four species, and two types of the overlapped-thickening guard cells and the arrested stomata exhibited commonly from seven to thirteen species. The another types of them rarely occurred from one to five species. Most of abnormal stomata were found in S. alboroseum, P. chinense and S. sarmentosum, and a few types of them were observed in the other species. The three types of the ballooning guard cells, the cross cleaved stomata, and the fused pore stomata were first reported here in the seed plants. It was thought that the abnormal stomata were formed by the result of degeneration and abnormal development of normal stomata.

• Journal of Korean Society for Atmospheric Environment
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• v.21 no.3
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• pp.367-375
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• 2005

Low-Z particle Electron Probe X-ray Microanalysis was applied to characterize MSW fly- and bottom -ash particle samples originated from two municipal incinerators (denoted as A and B) in Korea. According to their chemical composition, many distinctive particle types were identified. In A fly ash, the major chemical species are carbon-rich, aluminosilicates and many particles are composed of as a mixture of $ CaCO_3$ and other chemical species such as $CaSO_4$ or $CaCl_2$. For B fly ash, Fe, iron oxide, NaCl and NaCl-containing particles are the most abundant. In bottom ash, A and B were composed of similar chemical species such as carbon-rich, Fe, iron oxide, $CaCO_3$, and aluminosilicates. It was demonstrated that the single-particle characterization using this low-Z particle EPMA technique provided detailed information on various types of chemical species in the MSW ash samples. In addition, the technique has advantage over conventional analytical techniques in the point that both crystalline and glass-like ash particles can be analyzed at the same time.

• Korean journal of applied entomology
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• v.27 no.2
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• pp.81-86
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• 1988

Experiments were conducted to investigate the locational and ovipositional preferences of the single and mixed populations of the brown planthopper(BPH), Nilaparvata lugens (Stal), and the whitebacked planthopper(WBPH),Sogatella furcifera (Hovath), with the different popula-tion densities at seeding, max-tillering, booting and heading stages of rice variety seonam by대. The WBPH showed a locational preference for the upper portion while the BPH was observed to stay generally on the lower portion regardless of rice growth stages, population density-levels and the single or the mixed populations of two species. At seeding, max-tillerinf, boo-tring and heading stage, the preference for the lower portion of the rice plants was found to be slighty reduced with the lapse of time, respectively, from the single or the mixed popula-tions of both the species. Ovipositional lication of both the species was found not to be affected by the different population densities at rice growth stage. Although the BPH prefered a ovi-positional location for the lower portion at seeding, booting and heading stages, its preference-was somewhat reduced with the developmental stages of rice plants, whereas the WBPH showed a ovipositional preference for lower portion at seeding stages, but prefered the same tendency of ovipositional preference for the upper and the lower portion, The location and ovipositional preferences were likely to be affected by the competition between species with the mixed populations rather than single.

• Fisheries and Aquatic Sciences
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• v.24 no.5
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• pp.191-196
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• 2021

A single specimen of Liparis punctulatus (30.9 mm standard length) belonging to the family Liparidae, was collected in the waters off Busan on 4 August, 2016. This species is characterized by a single pair of nostrils, unlike other Liparis species. It is distinguished from L. ochotensis by the numbers of dorsal, anal, pectoral fin rays and the location of the gill opening (above the pectoral fin in L. punctulatus vs. extending ventrally in front of the pectoral fin). In the molecular analysis, 514 bp of 16S rRNA gene of the specimen in this study were identical to that of L. punctulatus from NCBI GenBank (K2P distance < 0.002). This is the first record of the species in Korea based on a voucher specimen, and we follow the Korean name "Kko-ma-kkom-chi" for this species proposed by Myoung et al. and Kim et al.

• Journal of Korean Society of Rural Planning
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• v.24 no.4
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• pp.57-67
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• 2018

We examined the impact of paddy wetland's avian-diversity on the development of the protected horticulture complex. The results of this study were made to propose ecological protected horticulture complex and development direction suitable for avian habitat. The total number of species surveyed was analyzed as 1168 individuals in 11 orders 26 families and 36 generic 48 species. Type average was analyzed as paddy $17.25{\pm}4.83$ species and $74.50{\pm}38.08$ individuals, glass $10.00{\pm}0.82$ species and $46.75{\pm}2.06$ individuals, single $13.75{\pm}7.27$ species and $59.50{\pm}35.34$ individuals, multi $8.75{\pm}0.96$ species and $36.75{\pm}9.29$ individuals. Paddy showed higher species diversity than protected horticulture complex. The average number of species surveyed for one year was glass $14{\pm}2.83$, multi $12{\pm}1.41$, single $18{\pm}11.31$, and paddy $26{\pm}11.31$. The diversity difference was identified as paddy> single> glass and multi. Statistical analysis showed statistical difference of Ciconiiformes. Development of protected horticulture complex causes loss of avian habitat. The diversity index was glass $1.73{\pm}0.07$, multi $1.68{\pm}0.14$, single $1.91{\pm}0.47$, and paddy $2.29{\pm}0.17$. Paddy has a higher species diversity than the protected horticulture complex. For the purpose of ecological protected horticulture complex, detention ponds, artificial wetlands and habitats should be reflected in the design. This should be applied to reclamation areas or large-scale protected horticulture complexes.