• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.276-276
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• 2013

Here we report an integrated microdevice consisting of an efficient passive mixer, a magnetic separation chamber, and a capillary electrophoretic microchannel in which DNA barcode assay, target pathogen separation, and barcode DNA capillary electrophoretic analysis were performed sequentially within 30 min for multiplex pathogen detection at the single-cell level. The intestine-shaped serpentine 3D micromixer provides a high mixing rate to generate magnetic particle-pathogenic bacteria-DNA barcode labelled AuNP complexes quantitatively. After magnetic separation and purification of those complexes, the barcode DNA strands were released and analyzed by the microfluidic capillary electrophoresis within 5 min. The size of the barcode DNA strand was controlled depending on the target bacteria (Staphylococcus aureus, Escherichia coli O157:H7, and Salmonella typhimurium), and the different elution time of the barcode DNA peak in the electropherogram allows us to recognize the target pathogen with ease in the monoplex as well as in the multiplex analysis. In addition, the quantity of the DNA barcode strand (~104) per AuNP is enough to be observed in the laser-induced confocal fluorescence detector, thereby making single-cell analysis possible. This novel integrated microdevice enables us to perform rapid, sensitive, and multiplex pathogen detection with sample-in-answer-out capability to be applied for biosafety testing, environmental screening, and clinical trials.

• Nuclear Engineering and Technology
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• v.41 no.1
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• pp.135-142
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• 2009

In this investigation, we prepared a 1 and 3-parallel serpentine single PEMFC, which has an active area of $100\;cm^2$ and a flow channel cross section of $1{\times}1mm$. Distribution and transport of water in a non-operating PEMFC were observed by varying flow types and the flow rates (250, 400, and 850 cc/min). This investigation was performed at the neutron imaging facility at the CO1d Neutron RAdiography facility (CONRAD), HMI, Germany of which the collimation ratio and neutron fluence rate are 250, $1{\times}10^{6}n/s/cm^2$, respectively. The neutron image was continuously recorded by a scintillator and lens-CCD coupled detector system every 10 seconds. It has been observed that although the distilled water was supplied into the cathode channel only, the neutron image showed a water movement from the cathode to the anode channel. The water at the cathode channel was completely discharged as soon as the pressurized air was supplied. But the water at the anode channel was not easily removed by the pressurized air except for the 3-parallel serpentine type with 850cc/min of air flow rate. Moreover, the water at the MEA wasn't removed for any of the cases.

• Journal of Hydrogen and New Energy
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• v.19 no.6
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• pp.467-473
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• 2008

This study is to investigate the influence of catalyst loading quantity on the direct methanol fuel cell (DMFC) performance. In this paper, Pt-Ru and Pt-black loading as the catalyst were varied from 1 to $4mg/cm^2$ at the anode and cathode, respectively. The experiment was conducted with single fuel cell consisted of $5cm^2$ effective electrode area, serpentine type flow pattern and Nafion 117 membrane. Also, AC impedance and methanol crossover current were measured to investigate the performance loss precisely. As a result, the performance of fuel cell was significantly increased with the increase of cathode catalyst loading. However, the performance did not increase further above a certain Pt-Ru catalyst loading as the increase of anode catalyst loading.

• Journal of Hydrogen and New Energy
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• v.15 no.4
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• pp.291-300
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• 2004

Performance of PEMFC is affected by many factors such as that of MEA, configuration of flow field, operating conditions, humidification, cooling and so on. In this study, in order to improve the performance of fuel cell, a small area fuel cell stack was made and its performance was tested under various operating conditions. Stack consists of 3 single PEM fuel cells. Channel is serpentine type and the active area of the electrode is $50cm^2$. The test results show that the peak power is 60W at $70^\circ{C}$ of stack temperature with humidification condition.

• Proceedings of the KSME Conference
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• 2008.11b
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• pp.2292-2297
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• 2008

Recently, a study on condensers for refrigerators has focused on new model which will cost less and will be more efficient. Some widely used condensers for domestic refrigerators are wire-and-tube type condenser, hot-wall type condenser, and spiral type condenser. Some companies which use the spiral type condenser at the moment try to develop a new type condenser which will cost less and will be as efficient as the spiral type. The new type condenser consists of a steel tube, steel plates and louver fins attached to the tube. The tube and the plate are bent into a single-passage serpentine shape.

• Journal of the Korean Society for Precision Engineering
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• v.25 no.1
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• pp.115-121
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• 2008

In this paper, a three-dimensional computational fluid dynamic model of a proton exchange membrane fuel cell(PEMFC) with serpentine flow channel is presented. A steady state, single phase and isothermal numerical model has been established to investigate the influence of the GDL (Gas Diffusion Layer) parameters. The GDL is made of a porous material such as carbon cloth, carbon paper or metal wire mesh. For the simplicity, the GDL is modeled as a block of material having numerous pathways through which gaseous reactants and liquid water can pass. The porosity, permeability and thickness of the GDL, which are employed in the model parameters significantly affect the PEMFC performance at the high current region.

• Proceedings of the KIEE Conference
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• 1999.07d
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• pp.1876-1878
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• 1999

Developed was a 5kW-class polymer electrolyte membrane fuel cell(PEMFC) system comprised of fuel cell stack, fuel processing, thermal and water management subsystems and ancillary equipments. Several large single cells have been fabricated with different gas flow field patterns and paths, and the gas flow field pattern for the stack has been determined based on the single cell performance of thin film membrane electrode assembly (MEA). The PEMFC stack was consisted of 100 cells with an electrode area of $300cm^2$, having serpentine flow pattern. Fuel processing was developed including an autothermal methanol reformer and two preferential CO oxidation reactors. The fuel processing was combined to PEMFC operation system consisted of air compressor and thermal and water management subsystems. The PEMFC stack showed performance of 5kW under the supply of $H_2$ and air, but its performance was lowered to 3.5kW under the supply of reformed gas.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.2
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• pp.318-327
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• 2002

In this paper, heat transfer and pressure oscillation characteristics on oscillating capillary tube heat pipe(OCHP) according to input heat flux, mixture ratio of working fluid and inclination angle were investigated and were compared single working fluid(R-142b) with binary mixture working fluid(R-142b-Ethano1). OCHP was made to serpentine structure of loop type with 10 turns by drilling the channels of length 220mm, width 1.5mm, and depth 1.5mm on the surface of brass plate. In this study, R-l42b and R-l42b-Ethanol were used as working fluids, the charging ratio of working fluids was 40(vol.%), the input heat flux to evaporating section was changed from 0.3W/㎠ to 1.8W/㎠, and mixture ratio of working fluid was R(100%), R(95%)-E(5%), R(90%)-E(10%), and R(85%)-E(15%). From the experimental results, it was found that the effective thermal conductivity of single working fluid was better than that of binary mixture working fluid. But, in case of binary mixture working fluid, critical heat flux was higher than that of single working fluid. And, the higher the mixture ratios of working fluid, the lower heat transfer performance. In case of pressure oscillation, as the inclination angle was lower, pressure wave was more irregular. These phenomena were more serious when the working fluid was binary mixture. Besides, when mixture ratio was higher, saturated pressure was increased, more irregular wave was observed and the mean amplitude was increased. For the same input heat flux, inclination angle and charging ratio, when pressure oscillation has sinusoidal wave, mean amplitude was small, and saturated pressure was low value, the heat transfer was excellent.

• Journal of Mechanical Science and Technology
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• v.17 no.9
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• pp.1358-1370
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• 2003

The effects of anode, cathode, and cooling channels for a Proton Exchange Membrane Fuel Cell (PEMFC) on flow fields have been investigated numerically. Continuous open-faced fluid flow channels formed in the surface of the bipolar plates traverse the central area of the plate surface in a plurality of passes such as a serpentine manner. The pressure distributions and velocity profiles of the hydrogen, air and water channels on bipolar plates of the PEMFC are analyzed using a two-dimensional simulation. The conservation equations of mass, momentum, and energy in the three-dimensional flow solver are modified to include electro-chemical characteristics of the fuel cell. In our three-dimensional numerical simulations, the operation of electro-chemical in Membrane Electrolyte Assembly (MEA) is assumed to be steady-state, involving multi-species. Supplied gases are consumed by chemical reaction. The distributions of oxygen and hydrogen concentration with constant humidity are calculated. The concentration of hydrogen is the highest at the center region of the active area, while the concentration of oxygen is the highest at the inlet region. The flow and thermal profiles are evaluated to determine the flow patterns of gas supplied and cooling plates for an optimal fuel cell stack design.

• Journal of the Korean Electrochemical Society
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• v.12 no.1
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• pp.61-67
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• 2009

Flow-field design has much influence over the performance of proton exchange membrane fuel cell (PEMFC) because it affects the pressure magnitude and distribution of the reactant gases. To obtain the pressure magnitude and distribution of reactant gases in five kinds of flow-field designs, computational fluid dynamics (CFD) analysis was performed. After the CFD analysis, a single cell test was carried out to obtain the performance values. As expected, the pressure differences due to different flow-field configurations were related to the PEMFC performance because the actual performance results showed the same tendency as the results of the CFD analysis. A large pressure drop resulted in high PEMFC performance. The single serpentine configuration gave the highest performance because of the high pressure difference magnitudes of the inlet/outlet. On the other hand, the parallel flow-field configuration gave the lowest performance because the pressure difference between inlet and outlet was the lowest.