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nif-Gene Organization and Nucleotide Sequence of nifV, nifH, D, K and nifE from Frankia Strain FaCl

  • An, Chung-Sun
    • Proceedings of the Zoological Society Korea Conference
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    • 1995.10b
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    • pp.120-120
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    • 1995
  • The total size of the pF AR1, a genomic clone of Frankia FaCI, was estimated to be about 44Kb by summation of the individual fragment length generated by single or double restriction enzymes. Southern hybridization analyses with Azotobacter vinelandii nif-genes as probes and partial sequencing analyses of the subclones revealed that organization of the nif-gene in the FaCI strain was nifV, H, D, K, E, N, X, W, B. The organization of the structural genes for nitrogenase is the same in this Frankia strain as it is in most other nitrogen-fixing prokaryotes but the positioning of the nifV-like gene relative to the nifHDK cluster differs. A consensus nif-promoter-like sequence, found at 5' of nifH, was not detected upstream of the niJV-like gene. nifV-like gene contained a ORF of 1206 NT encoding 401 amino acids. The nucleotide sequence and deduced amino acid sequence of the gene exhibit homology value of 65% and 41% with that from A vinelandii, respectively. The putative Shine-Dargamo sequences were present preceding nitK, nifH, D, K, and nifE, and in nitK gene putative start codon GTG was detected instead of A TG. The nucleotide and amino acid sequence of niIK of FaCI showed 82% and 76% homolgy with those of Frankia HFPCc 13, respectively. Amino acid sequence of niIK showed 69% and 61% homology with those of A vinelandii, Klebsiella pnewnoniae, respectively, while that of nifE 73% and 71%, respecti vely.i vely.

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The Real-Time Distance Relay Algorithm Using fault Location Estimation Information for Parallel Transmission Line (병행 2회선 송전선로에서 고장점 위치 추정정보를 이용한 실시간 거리계전 알고리즘)

  • 이재규;유석구
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.52 no.3
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    • pp.183-192
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    • 2003
  • This paper presents the real-time implemented distance relay algorithm which the fault distance is estimated with only local terminal information. When a single-phase-to-earth fault on a two-parallel transmission line occurs, the reach accuracy of distance relay is considerably affected by the unknown variables which are fault resistance, fault current at the fault point and zero- sequence current of sound line The zero-sequence current of sound line is estimated by using the zero sequence voltage which is measured by relaying location Also. the fault resistance is removed at the Process of numerical formula expansion. Lastly, the fault current through a fault point is expressed as a function of the zero-sequence current of fault line, zero-sequence current of sound line, and line, and fault distance. Therefore, the fault phase voltage can be expressed as the quadratic equation of the fault distance. The solution of this Quadratic equation is obtained by using a coefficient of the modified quadratic equation instead of using the square root solution method. After tile accurate fault distance is estimated. the mote accurate impedance is measured by using such an information.

An Efficient Code Acquisition Scheme for DSSS-based Real-Time Locating Systems

  • Lim, Jong-Tae;Park, Hyung-Rae
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.1A
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    • pp.41-48
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    • 2011
  • This paper proposes an efficient code acquisition scheme for direct-sequence spread spectrum (DSSS) based real-time locating systems (RTLS). The proposed scheme employs multiple bit integration to significantly decrease false alarms when no packet signal is transmitted and increase the detection probability when the RTLS tags are excited. The performance of the proposed code acquisition scheme is analyzed and numerically evaluated, and compared with those of single-dwell and traditional double-dwell code acquisition schemes. The simulation results show that the proposed multiple-bit integration scheme outperforms single-dwell and double-dwell schemes.

Determination of Mode I Fracture Toughness of Fiber Reinforced Composites by the Elastic Work Factor (섬유강화 복합재의 $G_ {IC}$ 결정을 위한 일인자방법)

  • Lee, Gyeong-Yeop;Go, Seung-Gi
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.20 no.11
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    • pp.3491-3497
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    • 1996
  • The work factor approach was applied to determine $G_ {IC}$ of fiber reinforced composites (AS4/3501) from a single unidirectional (0-deg) DCB specimen. Elastic work factors of DCB specimen for three different symmetrical staking sequences were derived from a simple bending theory and a finite element method. The results showed that elastic work factors calculated from both methods were comparable each other. In particular, the elastic work factor of DCB specimen with symmetrical stacking sequence is independent of stacking sequence. The $G_ {IC}$ determined from the work factor approach was compared with that determined by the compliance method. The results showed that the work factor approach and the compliance method produce comparable results of $G_ {IC}$. Thus, $G_ {IC}$ can be determined from a single DCB specimen using the work factor approach.

Precision Analysis of a Single Camera-based Depth Measurement System using the Reflected Images of a Rotating Mirror (회전 평면경의 반사 영상을 이용한 단일 카메라 시스템의 거리측정 정밀도 분석)

  • 나상익;손흥락;김형석
    • Proceedings of the IEEK Conference
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    • 2003.07e
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    • pp.2323-2326
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    • 2003
  • Theoretical analysis of the depth measurement system with the use of a single camera and a rotating mirror has hem done. A camera in front of a rotating mirror acquires a sequence of reflected images, from which depth information is extracted. For an object pint at a longer distance, the corresponding pixel in the sequence of images moves at a higher speed. In this paper, the principle d the depth measurement-based on the relation of the pixel movement speed and the depth of objects have been investigated. Also, necessary mathematics to implement the technique is derived and presented. The factors affecting the measurement precision have been studied Analysis shows that the measurement m increases with the increase of depth. The rotational angle of the mirror between two image-takings also affects the measurement precision. Experimental results using the real camera-mirror setup are reported.

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ON A SECURE BINARY SEQUENCE GENERATED BY A QUADRATIC POLYNOMIAL ON $\mathbb{Z}_{2^n}$

  • Rhee, Min-Surp
    • Journal of applied mathematics & informatics
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    • v.29 no.1_2
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    • pp.247-255
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    • 2011
  • Invertible functions with a single cycle property have many cryptographic applications. The main context in which we study them in this paper is pseudo random generation and stream ciphers. In some cryptographic applications we need a generator which generates binary sequences of period long enough. A common way to increase the size of the state and extend the period of a generator is to run in parallel and combine the outputs of several generators with different period. In this paper we will characterize a secure quadratic polynomial on $\mathbb{Z}_{2^n}$, which generates a binary sequence of period long enough and without consecutive elements.

Scheduling Algorithms for the Maximal Total Revenue on a Single Processor with Starting Time Penalty

  • Joo, Un-Gi
    • Management Science and Financial Engineering
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    • v.18 no.1
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    • pp.13-20
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    • 2012
  • This paper considers a revenue maximization problem on a single processor. Each job is identified as its processing time, initial reward, reward decreasing rate, and preferred start time. If the processor starts a job at time zero, revenue of the job is its initial reward. However, the revenue decreases linearly with the reward decreasing rate according to its processing start time till its preferred start time and finally its revenue is zero if it is started the processing after the preferred time. Our objective is to find the optimal sequence which maximizes the total revenue. For the problem, we characterize the optimal solution properties and prove the NP-hardness. Based upon the characterization, we develop a branch-and-bound algorithm for the optimal sequence and suggest five heuristic algorithms for efficient solutions. The numerical tests show that the characterized properties are useful for effective and efficient algorithms.

Al$_2$O$_3$ Growth control by sequence sampling (Sequence sampling에 의한 단결정(Al$_2$O$_3$) 성장 제어)

  • 조현섭;송용화;민병조
    • Proceedings of the KAIS Fall Conference
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    • 2000.10a
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    • pp.181-184
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    • 2000
  • It is a quite quality concerning to control temperature of single crystalline it does when you get most of heat treating Products. It is also important control temperature when you make the A1203(single crystalline) used to jewels, 91ass of watches. heat resistant transparent 91asses. Thus, it is a md to get the proper temperature in accordance with the time process while making mixture of oxygen and hydrogen to have the right temperature. In we will study of electrical valve positioning system for the gas mixture to im quality of Products.

Genetic Differentiation of Phytoplasma Isolates by DNA Heteroduplex Mobility Assay and Single-Strand Conformation Polymorphism Analysis

  • Cha, Byeongjin;Han, Sangsub
    • The Plant Pathology Journal
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    • v.18 no.6
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    • pp.308-312
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    • 2002
  • Heteroduplex mobility assay (HMA) and single-strand conformation polymorphism (SSCP) analyses combined with PCR were developed for genetic differentiation of various phytoplasma isolates. In the HMA and SSCP analyses, differences in the mobility shifts and the SSCP band patterns identified three distinct types of phyto-plasmas: Type Ⅰ, jujube witches'-broom (JWB) and ligustrum witches'-broom (LiWB); Type Ⅱ, mulberry dwarf(MD) and sumac witches'-broom (SuWB); and Type Ⅲ, paulownia witches'-broom (PaWB). Results of the sequence analyses revealed that phytoplasmas of JWB and MD had 100% homology with LiWB and SuWB, respectively. On the other hand, PaWB phyto-plasma had 97.8% homology with MD phytoplasma. The PCR-HMA and SSCP techniques were very useful in determining variations in sequence among several isolates of phytoplasmas. Furthermore, the methods were rapid, economical, highly sensitive, and easy to handle with the gels.

The complete chloroplast genome sequence of Dracocephalum rupestre (Lamiaceae)

  • Young-Soo KIM;Sang-Chul KIM;Young-Ho HA;Hyuk-Jin KIM
    • Korean Journal of Plant Taxonomy
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    • v.52 no.4
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    • pp.269-274
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    • 2022
  • Dracocephalum rupestre Hance is a perennial herb distributed across China, Mongolia, and Korea. This study reports the first complete chloroplast genome sequence of D. rupestre. The plastome is 151,230 bp long and exhibits a typical quadripartite structure comprising a large single-copy region of 82,536 bp, a small single-copy region of 17,408 bp, and a pair of identical inverted repeat regions of 25,643 bp each. It contains 130 genes, comprising 85 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analysis of D. rupestre and related species of Lamiaceae showed that the genus Dracocephalum is a monophyletic group, and D. rupestre is most closely related to D. psammophilum.