• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.6
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• pp.801-806
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• 2009

A species producing transglutaminase (EC 2.3.2.13) was isolated from forest soil and identified as Streptomyces platensis YK-2. The transglutaminase was purified from culture broth by 50% methanol precipitation, followed by successive chromatography on DEAE-Sephadex. The yield and purification-fold was 63.4% and 2.2-fold, respectively. The purified microbial transglutaminase (MTG) migrated as a single band of approximately 45 kDa upon sodium dodecyl sulfate polyacrylamide gel eletrophoresis. The isoelectric point determined by multichambered electrofocusing was pH $6.0{\sim}7.0$. The enzyme was strongly inhibited by $Hg^{++}$, but was activated by $Cd^{++}$, $Mg^{++}$, $Mn^{++}$, $Pb^{++}$ and reducing agents such as dithiothreitol and mercaptoethanol.

• Journal of Conservation Science
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• v.28 no.4
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• pp.411-416
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• 2012

The purpose of this study was to analyze AMS radiocarbon dating, using wiggle match, of a charcoal column excavated from a fired dwelling site (ra-#8) at the Pungnaptoseong earthen wall made in Baekje era. The result of wiggle matching for 6 decadal single-ring samples of the charcoal produced ${\pm}2{\sigma}$ radiocarbon date (95.4% confidence interval) as A.D. 190~280. It indicated that the dwelling site (ra-#8) belonged to the early and middle of the 3rd century. Radiocarbon dating results confirmed the date speculated by archaeologists according to dwelling structure and pottery style.

• Microbiology and Biotechnology Letters
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• v.13 no.4
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• pp.321-327
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• 1985

Cultivation conditions for the production of extracellular alkaline protease by a nonpiamentation Serratia sp. and purification of the enzyme were studied. The maximum enzyme level was obtained at the beginning of stationary phase when the organism was cultured on brain heart infusion medium at $25^{\circ}C$ under aeration (gyratory shaking, 180 cycles/min). The enzyme was purified about 100 fold with 16.5％ yield by ammonium sulfate precipitation, ammonium sulfate fractionation followed by DEAE-cellulose chromatography (1st and 2nd). The purified enzyme moved as a single symmetrical peak in the analytical ultracentrifuge. The enzyme demonstrated its maximum activity at pH 8.5-9.0 and 4$0^{\circ}C$ when vitamin-free casein was used as a substrate.

• Environmental Engineering Research
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• v.19 no.3
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• pp.289-292
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• 2014

The effect of carbon dioxide ($CO_2$) on global warming is serious problem. The adsorption with solid sorbents is one of the most appropriate options. In this study, the most interesting adsorbent is granular activated carbon (GAC). It is suitable material for $CO_2$ adsorption because of its simple availability, many specific surface area, and low-cost material. Afterwards, GAC was impregnated with chitosan solution as impregnated granular activated carbon (CGAC) in order to improve the adsorption capacity of GAC. This research aims to compare the physical and chemical characteristics of GAC and CGAC. The experiment was carried out to evaluate the efficiency of $CO_2$ adsorption between GAC and CGAC. The results indicated that the iodine number of GAC and CGAC was 137.17 and 120.30 mg/g, respectively. The Brunauer-Emmett-Teller results (BET) of both GAC and CGAC show that specific surface area was 301.9 and $531.3m^2/g$, respectively; total pore volume was 0.16 and $0.29cm^3/g$, respectively; and mean diameter of pore was 2.18 and 2.15 nm, respectively. Finally, the $CO_2$ adsorption results of both GAC and CGAC in single column how the maximum adsorption capacity was 0.17 and 0.25 mol/kg, respectively; how degeneration time was 49.6 and 80.0 min, respectively; and how the highest efficiency of $CO_2$ adsorption was 91.92% and 91.19%, respectively.

• The Plant Pathology Journal
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• v.21 no.4
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• pp.301-309
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• 2005

Trichoderma species/isolates exhibited varied degree of agglutination on sclerotial (Sc) and hyphal (Hy) surface of Macrophomina phaseolina. The agglutination efficiencies on Sc and Hy ranged from $11\;to\;57\%$. Isolates of T. harzianum (Th) and T. viride (Tv) showed greater agglutination on Sc ($23-57\%$) and Hy ($16-47\%$). Different enzymes (trypsin, pepsin, proteinase k, a-chymotrypsin, lyticase and glucosidase) and inhibitors (tunicamycin, cycloheximide, brefeldin A, sodium azide, dithiothreitol and SDS) reduced the agglutination potential of conidia of Th-23/98 and Tv-25/98; however, the extent of response varied greatly in different treatments. Different fractions of Th-23/98 and Tv-25/98 exhibited haemagglutinating reaction with human blood group A, B, AB and O. Haemagglutinating activity was inhibited by different sugars and glycoproteins tested. Crude haemagglutinating protein from outer cell wall protein fraction of Th-23/98 and Tv-25/98 were eluted on Sephadex G-100 column. Initially Th-23/98 and Tv-25/98 exhibited two peaks showing no agglutination activity; however, lectin activity was detected in the third peak. Similar to crude lectin, the purified lectin also exhibited haemagglutinating activity with different erythrocyte source. SDS-PAGE analysis of partially purified lectin revealed single band with an estimated molecular mass of 55 and 52 kDa in Th-23/98 and Tv-25/98, respectively. Trypsin, chymotrypsin and b-1,3-glucanase totally inhibited lectin activity. Similarly, various pH also affected the haemagglutinating activity of Th-23/98 and Tv-25/98. From the present observations, it can be concluded that the recognition/attachment of mycoparasite (T. harzianum and T. viride) to the host surface (M. phaseolina) may be most likely due to lectin-carbohydrate interaction.

• Horticultural Science & Technology
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• v.16 no.2
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• pp.233-235
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• 1998

Energized-functional water (EFW) and powder (EFP) were manufactured by Kyungwon Institute of Life Science, Seoul, through a series of processes; tap water ultra-purification energy imprinting with catalysts in platinum columns mixing energy-imprinted water + activated zeolites + photosynthetic bacteria fermenting at $25^{\circ}C$ filtering EFW and/or EFP. A single application of EFP to soil under tree canopy before bud burst, combined with three EFW applications to soil during growth of 'Fuji' apples (Malus domestica Borkh.) resulted in a higher Ca concentrations in fruit skins and flesh, and lower Ca and N concentrations in leaves and shoot-bark tissues. EFW also stimulated the net photosynthesis of leaves and root activity. Soluble solid concentrations (SSC) and anthocyanin levels of fruits were also significantly increased at harvest, producing greater firmness and less core browning during storage at $0^{\circ}C$. However, there was no significant difference in titratable acidity of fruit juice between the EFW treatment and the controls.

• Journal of the Earthquake Engineering Society of Korea
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• v.12 no.1
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• pp.43-55
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• 2008

The complexity of determining strain associated with shear modulus and damping ratio in torsional tests has been resolved by means of several approaches. Particularly, the modified equivalent radius approach is adequate to when generating the plots of equivalent radius ratio versus strain more effectively over any range of strains in resonant column and torsional shear (RC/TS) tests. The modified equivalent radius approach was applied for hyperbolic, modified hyperbolic, and Ramberg-Osgood models in evaluating damping ratio. Results showed that using a single value of equivalent radius ratio based on conventional equivalent radius approach is not appropriate. A new model was developed to consider the soil damping behavior at small strains as well as hysteretic damping and it was attempted to determine adjustments are required in evaluating strain associated damping when combining the two damping components.

• Journal of Sericultural and Entomological Science
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• v.28 no.1
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• pp.30-36
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• 1986

Polyacrylamide gel electrophoresis and autoradiography were applied to investigate the developmental profiles of the major hemplymph proteins (MHPs) and their biosynthesis. In addition, some biochemical methods were also used to isolate and purify the MHPs. The obtained results are summarized as follows. 1. MHP-a began to appear from the 2nd day of the fourth-instar larva while MHP-b and -c were detected first on the 1st day of the fifth-instar larva. All these proteins, however, showed a drastic increase in concentration at the 2nd day of the fifth-instar larva. 2. MHP-b and -c were synthesized in fat body on early day of the fifth-instar larva, but the possibility of MHP-a synthesis in fat body was excluded. 3. MHP-b was isolated and purified by heat-treatment (6$0^{\circ}C$), gel filtration on Sephadex G-100 and column chromatography on DEAE-cellulose and CM-cellulose. Purified MHP-b showed a single band on polyacrylamide gel- and SDS-polyacrylamide gel electrophoresis.

• Journal of People, Plants, and Environment
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• v.21 no.6
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• pp.485-492
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• 2018

This study aimed to develop and validate highly sensitive determination method of a prostaglandin ($PGE_1$, OP 1206) in human plasma by LC-MS/MS using column switching. Plasma stored at $-30^{\circ}C$ and treated with methanol effectively inhibited interferences synthesized post-sampling. Samples were added with internal standard and were separated by reversed-phase HPLC with a cycle time of 30min. The method was selective for OP 1206 and the regression models, based on internal standard, were linear across the concentration range 0.5-50 pg/mL with the limit of quantification of 0.5 pg/mL (limit of quantitation, LOQ) for OP 1206. The calibration curve of OP 1206 standards spiked in five individual plasma samples was linear ($r^2=0.9999$). Accuracy and precision at the concentrations of 0.5, 1.5, 5.0 and 40 pg/mL, and at the lower LOQ of 0.5 pg/mL were excellent at 20%. OP120 < 6 was stable in plasma samples for at least 24 hours at room temperature, 24 hours frozen at $-70^{\circ}C$, 24 hours in an auto sampler at $6^{\circ}C$, and for two freeze/unfreezing cycles. The validated determination method successfully quantified the concentrations of OP 1206 in plasma samples from simulated administrating a single $5{\mu}g$ OP 1206 formulation. Thus, this novel LC-MS/MS technique for drug separation, detection and quantitation is expected to become the standard highly-sensitive detection method in bioanalysis and to be applied to many low dose pharmaceutical products.

• Earthquakes and Structures
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• v.16 no.2
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• pp.185-196
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• 2019

One of the shortcomings of seismic bridge design codes is the lack of clarity in defining the role of different seismic isolation systems with linear or nonlinear behavior in terms of R-factor. For example, based on AASHTO guide specifications for seismic isolation design, R-factor for all substructure elements of isolated bridges should be half of those expressed in the AASHTO standard specifications for highway bridges (i.e., R=3 for single columns and R=5 for multiple column bent) but not less than 1.50. However, no distinction is made between two commonly used types of seismic isolation devices, i.e., elastomeric rubber bearing (ERB) with linear behavior, and lead rubber bearing (LRB) with nonlinear behavior. In this paper, five existing bridges located in Iran with two types of deck-pier connection including ERB and LRB isolators, and two bridge models with monolithic deck-pier connection are developed and their R-factor values are assessed based on the Uang's method. The average R-factors for the bridges with ERB isolators are calculated as 3.89 and 4.91 in the longitudinal and transverse directions, respectively, which are not in consonance with the AASHTO guide specifications for seismic isolation design (i.e., R=3/2=1.5 for the longitudinal direction and R=5/2=2.5 for the transverse direction). This is a clear indicator that the code-prescribed R-factors are conservative for typical bridges with ERB isolators. Also for the bridges with LRB isolators, the average computed R-factors equal 1.652 and 2.232 in the longitudinal and transverse directions, respectively, which are in a good agreement with the code-specified R-factor values. Moreover, in the bridges with monolithic deck-pier connection, the average R-factor in the longitudinal direction is obtained as 2.92 which is close to the specified R-factor in the bridge design codes (i.e., 3), and in the transverse direction is obtained as 2.41 which is about half of the corresponding R-factor value in the specifications (i.e., 5).