• Journal of Korean Society of Water and Wastewater
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• v.23 no.5
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• pp.599-608
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• 2009

This manuscript covers the results of field investigation and lab-scale experiments to design a double-layered biofilter system to control urban storm runoff. The biofilter system consisted of a coarse soil layer (CSL) for filtration and fine soil layer (FSL) for adsorption and biological degradation. The variations of flow rate and water quality of runoff from a local expressway were monitored for seven storm events. Laboratory column experiments were performed using seven kinds of soil and mulch to maximize pollutants removal. The site mean concentration (SMC) of storm runoff from the drainage area (runoff coefficient: 0.92) was measured to be 203 mg/L for SS, 307 mg/L for $TCOD_{Cr}$, 12.3 mg/L for TN, 7.3 mg/L for ${NH_4}^+-N$, and 0.79 mg/L for TP, respectively. This study employed a new design concept, to cover the maximum rainfall intensity with one month recurrence interval. Effective storms for last ten years (1998-2007) in seoul suggested the design rainfull intensity to be 8.8 mm/hr Single layer soil column showed the maximum removal rate of pollutants load when the uniformity coefficient of CSL was 1.58 and the silt/clay contents of FSL was virtually 7%. The removal efficiency during operation of double layer soil column was 98% for SS and turbidity, 75% for TCODCr, 56% for ${NH_4}^+-N$, 87% for TP, and 73-91% for heavy metals. The hydraulic conductivity of the soil column, 0.023 cm/sec, suggested that the surface area of the biofilter system should be about 1% of the drainage area to treat the rainfall intensity of one month recurrence interval.

• Structural Engineering and Mechanics
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• v.22 no.2
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• pp.169-184
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• 2006

A close form solution of the maximum deflection for cracked columns with rectangular cross-sections was developed and thus the elastic buckling behavior and ultimate bearing capacity were studied analytically. First, taking into account the effect of the crack in the potential energy of elastic systems, a trigonometric series solution for the elastic deflection equation of an arbitrary crack position was derived by use of the Rayleigh-Ritz energy method and an analytical expression of the maximum deflection was obtained. By comparison with the rotational spring model (Okamura et al. 1969) and the equivalent stiffness method (Sinha et al. 2002), the advantages of the present solution are that there are few assumed conditions and the effect of axial compression on crack closure was considered. Second, based on the above solutions, the equilibrium paths of the elastic buckling were analytically described for cracked columns subjected to both axial and eccentric compressive load. Finally, as examples, the influence of crack depth, load eccentricity and column slenderness on the elastic buckling behavior was investigated in the case of a rectangular column with a single-edge crack. The relationship of the load capacity of the column with respect to crack depth and eccentricity or slenderness was also illustrated. The analytical and numerical results from the examples show that there are three kinds of collapse mechanisms for the various states of cracking, eccentricity and slenderness. These are the bifurcation for axial compression, the limit point instability for the condition of the deeper crack and lighter eccentricity and the fracture for higher eccentricity. As a result, the conception of critical transition eccentricity $(e/h)_c$, from limit-point buckling to fracture failure, was proposed and the critical values of $(e/h)_c$ were numerically determined for various eccentricities, crack depths and slenderness.

• Korean Journal of Microbiology
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• v.25 no.3
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• pp.180-183
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• 1987

Stu I, type II restriction endonuclease, has been purified to homogeneity from Streptomyces tubercidicus (ATCC 25502), and its catalytic properties have been studied. For the purification of Stu I endonuclease free of nonspecific nucleases, DEAE-Sephadex (A-50), QAE-Sephadex (A-50) and Heparin-agarose column chromatography have been performed after ammonium sulfate fractionation of the crude extract. The enzyme was further purified by gel filtration using Sephadex G-100 column to obtain homogeneous form of protein. The single polypeptide species of Stu I endonuclease has a subunit molecular weight of 34,000 $\pm$ 1,000 daltons as judged by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Stu I endonuclease requires $Mg^{2+}$ ion for its activity and is maximally active at neutral pH (7.0-8.0) in the absence of NaCl.

• Korean Journal of Food Science and Technology
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• v.16 no.3
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• pp.322-328
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• 1984

These experiments were conducted to purify the glucoamylase produced by Rhizopus oryzae. Two forms of glucoamylase (GI and GII) from Phizopus oryzae were purified by $(NH_2)_2SO_4$ fractionation, acetone fractionation and successive column chromatography on DEAE-cellulose and CM-cellulose. The specific activities of GI and GII toward soluble starch were 157.6 U/㎎. protein (37.5 fold of crude extract), and 164.7 U/㎎. protein (39.2 fold of curde extract), respectively, and the yields of them were 4.3% and 3.8%, respectively. The two purified enzymes have shown a single band by polyacrylamide disc gel electrophoresis and SDS-polyacrylamide gel electrophoresis. The protein bands of their electrophoresis gel were revealed to have glucoamylase activity by iodine staining and were proved to be glycoprotein by periodic acid Schiff's staining.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.2
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• pp.312-315
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• 2000

To purify the macrophage phagocytic activity-enhancing component, ethanol-acetic acid (100 : 1, EA) extract of Korean wheat (Gobun wheat) and imported one (Australian Standard White, ASW) were fractionated with ethylacetate : methanol : $H_2O$(65 : 25 : 4, v/v/v), and identified by TLC and column chromatography. At least five fractions were separated from the EA extract of the wheats but amounts of fraction B, C and D were more in Gobun wheat than in ASW. The effects of all fractions on phagocytic activity were tested in macrophage J774 cells. Among the fractions, only fraction b of Gobun wheat showed significant increase of phagocytic activity against yeast.

• Earthquakes and Structures
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• v.23 no.5
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• pp.473-491
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• 2022

The research investigates experimentally the effect of confinement on structural behavior at the ends of beam-column in reinforced concrete (RC) frames. In the experimental study, five specimens consisting of 1/3-scaled RC frames having single-bay, representing the traditional deficiencies of existing buildings constructed without receiving proper engineering service is investigated. The RC frame specimens were produced to represent most of the existing buildings in Turkey that have damage potential. To decrease the probable damage to the existing buildings exposed to earthquakes, the carbon Textile Reinforced Mortar (TRM) strengthening technique (fully wrapping) was used on the ends of the RC frame elements to increase the energy dissipation and deformation capacity. The specimens were tested under reversed cyclic lateral loading with constant axial loads. They were constructed satisfying the weak column-strong beam condition and consisting of low-strength concrete, such as compressive strength of 15 MPa. The test results were compared and evaluated considering stiffness, strength, energy dissipation capacity, structural damping, ductility, and damage propagation in detail. Comprehensive investigations of these experimental results reveal that the strengthening of a brittle frame with fully-TRM wrapping with non-anchored was effective in increasing the stiffness, ductility, and energy dissipation capacities of RC bare frames. It was also observed that the frame-only-retrofitting with an infill wall is not enough to increase the ductility capacity. In this case, both the frame and infill wall must be retrofitted with TRM composite to increase the stiffness, lateral load carrying, ductility and energy dissipation capacities of RC frames. The presented strengthening method can be an alternative strengthening technique to enhance the seismic performance of existing or moderately damaged RC buildings.

• Structural Engineering and Mechanics
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• v.90 no.6
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• pp.577-590
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• 2024

Considering the Column-Supported Group Silos (CSGSs) often arranged by rows in practical applications, earthquake responses will be affected by group effect. Since group effect presenting uncertainties, establishing the analytic model and evaluating characteristics of CSGSs seems necessary. This study aimed at providing a simplified method to evaluate seismic performances of the CSGSs. Firstly, the CSGSs with different storage granule heights are used as numerical examples to derive the base shear formula for three-particle dynamic analytical model. Then, the base shear distribution coefficient is defined as the group effect index. The simplified calculation method of the group silos based on the distribution coefficients is proposed. Finally, based on the empty, half, and full granular storage conditions, the empirical design parameters for the group silos system are given by combining finite element simulation with shaking table test. The group effect of storage granule heights of group silos on its frequency and base shear are studied by comparative analysis between group silos and independent single silo. The results show that the frequency of CSGSs decreases with the increasing weight of the stored granule. The connection between the column top and silo bottom plate is vulnerable, and structural measures should be strengthened to improve its damage resistance. In case of different storage granule heights, distribution coefficients are effective to reconstruction the group effect. The complex calculations of seismic response for CSGSs can be avoided by adopting the empirical distribution coefficients obtained in this study. The proposed method provides a theoretical reference for evaluation on the seismic performances of the CSGSs.

• BMB Reports
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• v.30 no.3
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• pp.177-181
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• 1997

We constructed a single-chain immunoglobulin in which the carboxyl end of the heavy chain variable domain is covalently joined to the amino terminus of the light chain variable domain via peptide linker and the carboxyl end of the light chain variable domain is linked to human ${\gamma}1$ Fc region through the hinge region. The molecule was expressed in Chinese hamster ovary cells, assembled into a dimeric molecule and secreted into the culture medium. The dimeric molecule (2E11) was purified from the culture supernatant by affinity chromatography on Protein G-Sepharose column. The size of the unreduced or reduced protein was the expected molecular weight of approximately 120 or 60 kDa, respectively, as assessed by SDS-polyacrylamide gel electrophoresis. The antigen-binding affinity of 2E11 was almost the same as that of a native antibody counterpart (CS131A), suggesting that the single-chain immunoglobulin may function like a native antibody.

• Applied Biological Chemistry
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• v.49 no.1
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• pp.25-29
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• 2006

This study was carried out to isolate the possible anti-tumor promoters from the citrus peel (Citrus natsudaidai Hayata). We fractionated the cold-pressed oil of citrus peel by column chromatography, HPLC and TLC. The analysis on column chromate-graphy yielded seven peaks $(F-I{\sim}F-VII)$, all of which showed single spot on TLC analysis ($R_f$ for $F-I{\sim}VIII$; 0.31, 0.13, 0.13, 0.78, 0.79, 0.69 and 0.84). Among the seven fractions, three fractions (F-I, -II and F-IV) were re-analyzed on HPLC, also showing single peak except for one fraction (F-IV) which was divided two peaks. The retention times $(R_f)$ of F-I and F-II was 3 min. and 2.5 min., respectively, but these of two peaks from F-IV were 2 min. and 4.5 min., respectively. Since the area of the latter peak (4.5 min.) was very smaller than that of the former one (2 min.), it is considered that the latter one did not appear on TLC analysis. The inhibitory effect on tumor promoter 12-O-tetradecanoylphorbol-13-acetate(TPA)-induced Epstein-Barr virus activation in Raji cells was tested for the seven fraction obtained. It decreased in order of F-VI (82.3+1.3%) > F-I (80.4+1.6%) > F-II (77.2+0.9%) > F-III (75.0+1.2%) > F-IV (74.1=1.0%) > F-V (71.0+1.1%) > F-VII (70.2+1.2%). These results imply that some constiuents contained in citrus peels have the inhibitory activity of TPA-induced tumor promotion.

• Journal of Soil and Groundwater Environment
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• v.13 no.4
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• pp.54-61
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• 2008

Permeable reactive barriers (PRBs) technology has been focused in contaminated groundwater remediation. It is necessary to select adequate reactive material according to characteristics of contaminant in groundwater. In this research, the reaction between reactive material and heavy metal contaminants was estimated through column test. Reactive material was slag, which has been produced in Gwangyang power plant, and heavy metal contaminants were cadmium, lead and copper. Column test was performed in the condition of 1) single and multi contaminated solution and 2) different initial concentration of cadmium. Retardation factor of cadmium is 3.94 in multi contamination. But that of copper is 40.3 in single and 25 in multi. The difference of retardation between cadmium and copper is due to affinity, resulted from the difference of electronegativity. In multi-contamination, copper effluent concentration was above initial copper concentration and at the same time lead effluent concentration was decreased. This phenomenon was considered that lead extract copper sorbed in slag and then lead was sorbed to the vacant sorption site instead. And as the initial concentration was increased, the retardation factor of cadmium became decreased.