• The Korean Journal of Zoology
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• v.29 no.4
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• pp.283-293
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• 1986

For the preliminary step to make and characterize the monoclonal antibodies of human alpha-fetoprotein (AFP) was purified from 534g of human fetal tissues through the procedures of tissue extraction, DEAE-cellulose, concanavalin A-Sepharose, Cibacron Blue F3GA-agarose and immunoadsorbent affinity chromatography. The isolated AFP preparation showed a single band on polyacrylamide gel electrophoresis and a single precipitin are against rabbit anti-human cord serum and anti-human AFP on immunoelectrophoresis. Our AFP also displayed a single band on SDS-polyacrylamide gel electrophoresis. The recovery of AFP was 8.76mg total.

• Journal of Korea Multimedia Society
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• v.7 no.6
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• pp.781-790
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• 2004

This paper presents a fourth-order cumulants based iterative algorithm for blind channel equalization. It is robust with respect to the existence of heavy Gaussian noise in a channel and does not require the minimum phase characteristic of the channel. In this approach, the transmitted signals at the receiver are over-sampled to ensure the channel described by a full-column rank matrix. It changes a single-input/single-output (SISO) finite-impulse response (FIR) channel to a single-input/multi-output (SIMO) channel. Based on the properties of the fourth-order cumulants of the over-sampled channel outputs, the iterative algorithm is derived to estimate the deconvolution matrix which makes the overall transfer matrix transparent, i.e., it can be reduced to the identity matrix by simple reordering and scaling. Both a closed-form and a stochastic version of the proposed algorithm are tested with three-ray multi-path channels in simulation studies, and their performances are compared with a method based on conventional second-order cumulants. Relatively good results are achieved, even when the transmitted symbols are significantly corrupted with Gaussian noise.

• Environmental Engineering Research
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• v.24 no.1
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• pp.31-37
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• 2019

In this paper the results of the biosorption of lead(II) and cadmium(II) with sugarcane bagasse in fixed bed columns are presented. Experimental data were fitted to several models describing the rupture curve for single-component and two-component systems. The percentages of removal of lead and cadmium in single-component systems are 91% and 90%, respectively. In lead-cadmium bicomponent systems the percentage of elimination of lead was 90% and cadmium 92%. In single-component systems, Yoon-Nelson and Thomas models successfully reproduce the rupture curves. In two-component system, the Dose-Response model was the best one reproducing the experimental rupture curves in the entire measured range.

• Geotechnical Engineering
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• v.6 no.1
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• pp.7-14
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• 1990

The two degree of freedom model is proposed to study the effects of imperfect fixing at the active end of spring-top resonant column apparatus. A computer program using the SYMPHONY spreadsheet is developed to calculate the dimensionless frequency, F, from which modulug can be determined. It is found that the effect of reaction mass through the parameter Tr on dimensionless frequency, F, can not be ignored if Tr$\leq$20. As To increases, the variation of F increases. But for Tr$\geq$ 20, the effect of To becomes small. It is recommended that T. be greater than 20 if single degree of freedom model is rosed to determine modulus of soil. It also is found that damping ratios of specimen and apparatus do not strongly affect the dimensionless frequency, F.

• KCI Concrete Journal
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• v.14 no.3
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• pp.102-110
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• 2002

Results of an experimental study on the pullout behavior of the headed reinforcement are presented. A total of 48 pullout tests was performed to evaluate pullout strengths and load-displacement behaviors in pullout of the headed bars. The square steel heads had gross area of 4 $A_{b}$ and thickness of $d_{b}$ The test program consisted of three pullout test groups: Simple and Edge pullout tests using plain concrete slabs, comparison of pullout performances between the standard hooks and the headed reinforcement, and pullout tests of headed reinforcement using reinforced concrete columns. Test variables included concrete strengths ( $f_{c}$' = 27.1MPa, 39.1MPa), reinforcing bar diameters (D16~D29), embedment depths (6 $d_{b}$~12 $d_{b}$), edge conditions, column reinforcement, and single-vs.-multiple bar pullout. Test results revealed that the heads effectively provided the pullout resistances of the deformed bars in tension. The load-displacement behaviors were similar between the 90-degree hooks and the headed reinforcement. When a multiple number of headed bars installed with small head-to-head spacings was pulled out, reinforcement designed to run across the concrete failure surface in a direction parallel to the headed bars helped improve the pullout performances of the headed reinforcement.t.ement.t.

• Electrical & Electronic Materials
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• v.8 no.3
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• pp.272-277
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• 1995

We report the properties of optically pumped stimulated emission at room temperature (RT) from column-III nitride semiconductors of GaN, AlGaN/GaN double heterostructure (DH) and AlGaN/GaInN DH which prepared on a sapphire substrate using an AIN buffer-layer by the nietalorganic vapor phase epitaxy (MOVPE) method. The peak wavelength of the stimulated emission at RT from AIGaN/GaN DH is 369nm and the threshold of excitation pumping power density (P$\_$th/) is about 84kW/cm$\^$2/, and they from AlGaN/GaInN DH are 402nm and 130kW/cm$\^$2/ at the pumping power density of 200kW/cm$\^$2/, respectively. The P$\_$th/ of AIGaN/GaN and AlGaN/GaInN DHs are lower than the single layers of GaN and GaInN due to optical confinement within the active layers of GaN and GaInN, respectively.

• KSBB Journal
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• v.14 no.3
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• pp.322-327
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• 1999

The cholesterol oxidase(EC.1.1.3.6) produced from Streptomyces sp. No.4 which isolated from soil was purified and investigated for the enzymatic properties. The enzyme was purified specifically by cholesterol affinity column chromatography with a yield of 28.3%. The purified enzyme showed a single polypeptide on SDS-PAGE and the molecular weight was estimated to be 60,000 daltons. The enzyme activity was strongly inhibited by metal ions such as $HgCl_2$ and $CuSO_4$. Dithiothreitol and mercaptoethanol inhibited the enzyme activity at concentration of 1mM. The Michaelis constant(Km) for cholesterol was found to be 1.38mM by Lineweaver-Burk plot analysis. Amino acid analysis showed that the enzyme protein was composed of 416 amino acid residues including 52moles of glycine and 19moles of tryptophane.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.6
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• pp.833-838
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• 1993

A strain of Penicillium sp. extracellularly produced an inhibitory substance for lipoxygenase. These purification procedures were followed : ethanol treatment, chromatographies on Dowex 50W, Sephadex G-25, silica gel column and HPLC. The inhibitor was stable in pH range from 3.0 to 5.0 at $25^{\circ}C$, and a treatment at 10$0^{\circ}C$ for 2 hours didn't diminish its original activity. The purified inhibitor was charred at temperature near 22$0^{\circ}C$~23$0^{\circ}C$ and decomposed. Molecular weight of the inhibitor was estimated to be approximately 270 by Sephadex G-25 column chromatography. The inhibitor rapidly formed EI complex with lipoxygenase and inhibited enzyme activity.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2006.04a
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• pp.20-28
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• 2006

Partitioning tracer tests were conducted to quantify the saturation degree of diesel and water in unsaturated soil, respectively. The use of partitioning tracers that partition into diesel, water, and air (i.e., three-phase partitioning), is in attractive alternative to traditional coring and analysis method. These gaseous partition tracers not global warming gas like CFC's are Butane, Acetylene, Ethylene, Methylene chloride, and Methane. The glass column packed with sandy soil was prepared, in which a three-phase system of air, water, and diesel was maintained. Conservative and partition gas tracers were injected into the columns and detected easily using a single GC detector(FID). For each tracer, a method of moments was used to estimate partition coefficient between water, diesel. and the air, respectively. The results from the column studies showed that the diesel/air tracer partition coefficient ranged from 8.2 to 868 and 9.2 for water/air. Saturation degree of diesel and water in the column, predicted by the partition coefficients obtained from tests, was underestimated up to 66% and 23% respectively.

• Journal of ILASS-Korea
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• v.9 no.2
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• pp.34-40
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• 2004

In a liquid-fueled ramjet engine, the insufficient mixing and evaporation result in the low combustion efficiency and combustion instability. Improving its characteristics and devising a means of fuel droplets with air may compensate these disadvantages of liquid fuel ramjet engine. The jet penetrations of various fuel injectors were measured to investigate the spray characteristics of a liquid-fueled ramjet engine under high pressure air-stream conditions. The penetrations in high pressure conditions are smaller than the values calculated from Inamura's or Lee's equations, and the jet penetrations in the high pressure conditions have a similar tendency. In the dual orifice injectors, the jet penetrations of rare orifice is rapidly increased due to the reduction of the drag, which is created by the jet column of front orifice. The jet penetration of rare orifice is increased because of the drag reduction created by the jet column of the front orifice. Because of the drag reduction formed by the column of jet, the jet penetration in the rear orifice of dual orifice injector is much larger than the jet penetrations of single orifice injector. As the distances of the orifice are increased, the jet penetrations of the rear orifice decrease.