• Tuberculosis and Respiratory Diseases
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• v.86 no.4
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• pp.294-303
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• 2023

Background: The human lung serves as a niche for a unique and dynamic bacterial community related to the development and aggravation of multiple respiratory diseases. Therefore, identifying the microbiome status is crucial to maintaining the microecological balance and maximizing the therapeutic effect on lung diseases. Therefore, we investigated the histological type-based differences in the lung microbiomes of patients with lung cancer. Methods: We performed 16S rRNA sequencing to evaluate the respiratory tract microbiome present in bronchoalveolar lavage fluid. Patients with non-small cell lung cancer were stratified based on two main subtypes of lung cancer: adenocarcinoma and squamous cell carcinoma (SqCC). Results: Among the 84 patients analyzed, 64 (76.2%) had adenocarcinoma, and 20 (23.8%) had SqCC. The α- and β-diversities showed significant differences between the two groups (p=0.004 for Chao1, p=0.001 for Simpson index, and p=0.011 for PERMANOVA). Actinomyces graevenitzii was dominant in the SqCC group (linear discriminant analysis [LDA] score, 2.46); the populations of Haemophilus parainfluenza (LDA score, 4.08), Neisseria subflava (LDA score, 4.07), Porphyromonas endodontalis (LDA score, 3.88), and Fusobacterium nucleatum (LDA score, 3.72) were significantly higher in the adenocarcinoma group. Conclusion: Microbiome diversity is crucial for maintaining homeostasis in the lung environment, and dysbiosis may be related to the development and prognosis of lung cancer. The mortality rate was high, and the microbiome was not diverse in SqCC. Further large-scale studies are required to investigate the role of the microbiome in the development of different lung cancer types.

• Journal of Microbiology and Biotechnology
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• v.11 no.5
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• pp.788-797
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• 2001

The usefulness of three PCR methods were evaluated for the epidemiological typing of Staphylococcus aureus: an enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR), repetitive extragenic palindromic element PCR (REP-PCR), and 16S-23S intergenic spacer PCR (ITS-PCR). The analysis was performed using a collection of S. aureus strains comprised of 6 reference and 79 isolates from patients with various diseases. Among the 85 S. aureus strains tested, 6 references and 6 isolates were found to be susceptible to methicillin, whereas the remaining 73 isolates were resistant to it. PCR methods are of special concern, as conventional phenotypic methods are unable to clearly distinguish among methicillin-resistant S. aureus (MRSA) strains. The ability of the techniques to detect different unrelated types was found to be as follows: ERIC-PCR, 19 types; REP-PCR, 36 types; and ITS-PCR, 14 types. On the basis of combining the ERIC, REP, and ITS fingerprints, the 85 S. aureus strains were grouped into 56 genetic types (designated G1 to G56). The diversities for the 85 S. aureus strains, calculated according to Simpson\`s index, were 0.88 for an ERIC-PCR, 0.93 for a REP-PCR, and 0.48 for an ITS-PCR, and the diversity increased up to 0.97 when an ERIC-PCR and REP-PCR were combined. The above discrimination indices imply that the genetic heterogeneity of S. aureus strains is high. Accordingly, this study demonstrates that DNA sequences from highly conserved repeats of a genome, particularly a combination of ERIC sequences and REP elements, are a convenient and accurate tool for the subspecies-specific discrimination and epidemiologic tracking of S. aureus.

• Mycobiology
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• v.45 no.3
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• pp.160-171
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• 2017

Larvae of Bradysia agrestis, an insect vector that transports plant pathogens, were sampled from geographically isolated regions in Korea to identify their cutaneous fungal and bacterial flora. Sampled areas were chosen within the distribution range of B. agrestis; each site was more than 91 km apart to ensure geographical segregation. We isolated 76 microbial (fungi and bacteria) strains (site 1, 29; site 2, 29; site 3, 18 strains) that were identified on the basis of morphological differences. Species identification was molecularly confirmed by determination of universal fungal internal transcribed spacer and bacterial 16S rRNA gene sequences in comparison to sequences in the EzTaxon database and the NCBI GenBank database, and their phylogenetic relationships were determined. The fungal isolates belonged to 2 phyla, 5 classes, and 7 genera; bacterial species belonged to 23 genera and 32 species. Microbial diversity differed significantly among the geographical groups with respect to Margalef's richness (3.9, 3.6, and 4.5), Menhinick's index (2.65, 2.46, and 3.30), Simpson's index (0.06, 0.12, and 0.01), and Shannon's index (2.50, 2.17, and 2.58). Although the microbial genera distribution or diversity values clearly varied among geographical groups, common genera were identified in all groups, including the fungal genus Cladosporium, and the bacterial genera Bacillus and Rhodococcus. According to classic principles of co-evolutionary relationship, these genera might have a closer association with their host insect vector B. agrestis than other genera identified. Some cutaneous bacterial genera (e.g., Pseudomonas) displaying weak interdependency with insect vectors may be hazardous to agricultural environments via mechanical transmission via B. agrestis. This study provides comprehensive information regarding the cutaneous microflora of B. agrestis, which can help in the control of such pests for crop management.

• Korean Journal of Agricultural and Forest Meteorology
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• v.5 no.3
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• pp.151-157
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• 2003

This research was conducted to investigate about structural characteristics of vegetation by change of the environmental factors for prolific open-pollinated progeny forest in Pinus koraiensis stands. Stand slope of Kapyung area was slower than Youngdong area. On the other hand, contents rate of the soil chemical properties in Kapyung was lower than Youngdong area. And relative intensity of light was higher Youngdong than Kapyung area. On the whole, growth of the Pinus koraiensis was more dominant Kapyung area than Youngdong. Analysis result of the relative density, - frequency, - coverage and importance value to shrub layer in each local stands, Quercus mongolica and Quercus acutissima were dominant species in Kapyung area. Dominant species In Youngdong area was Quercus acutissima. In Kapyung area, appearance species of the vegetation layer was consisted that live in moist soil and shaded lot. Youngdong area showed fewer species than Kpyung area. Species diversity of shrub and vegetation layer in Kapyung and Youngdong area was higher than Youngdong. This result was judged by slope difference between each areas. Kapyung area need enforce thinning and Youngdong area conduct tending management on shrub layer.

• Korean Journal of Weed Science
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• v.31 no.1
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• pp.89-95
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• 2011

In present study we focused on the weed occurrence and diversity in rice field according to methods of weed control. Experiments comprised of no herbicide treatment (NHT), herbicide treatment (HT) and golden apple snail treatment (GAST) within the three months (July to September) of growing rice. According to results, five to seven different weed species were found in NHT, while two to four different weed species were found in HT and GAST. Monochoria vaginalis was dominant species during growing period in NHT and HT while Lemna paucicostata was the dominant in July but Persicaria hydropiper was dominant for August and September. Simpson's dominance index was highest in GAST(0.96) compared with other treatments (NHT 0.27 and HT : 0.51). The similarity coefficient was 50.31%, 4.65% and 0.38% for NHT versus HT, NHT versus GAST and HT versus GAST, respectively. In conclusion, the weed species diversity, dominance and similarity coefficient were varied in different weed control methods of rice growing.

• Korean Journal of Environmental Biology
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• v.38 no.1
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• pp.146-159
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• 2020

The objective of our study was to analyze the distribution and composition of fish species and their community characteristics in agricultural waterways in the six water systems-Mankyung River, Seomjin River, Namhae Region, Tamjin River, Yeongsan River, and Dongjin River-during 2017-2018. Little is known about the distributions and community characteristics of the aquatic biota of agricultural waterways in Korea, even if the water bodies have important roles as spawning areas or habitats for fish. In this study, we identified 3,633 fish individuals in the waterways, belonging to 53 species, eleven families, and six orders (dominant species: Tanakia koreensis, T. lanceolate) and 7,108 fish individuals in the main rivers, belonging to 57 species, fifteen families, and nine orders. Moreover, three endangered wildlife (designated by the Ministry of Environment, Korea)-T. somjinensis, Acheilognathus majusculus and Coreoperca kawamebari-were found in the waterways. By fish guild analysis, we found that many waterways had a lower proportion of sensitive species and a higher proportion of omnivore species than those of the main rivers, which indicates a feature of a disturbed environment. In addition, by grouping with environmental variables, we found the group of waterways with high substrate complexity had a higher diversity index (Shannon index) and a lower dominance index (Simpson index) than that of simple substrate complexity. Overall, our study represents the characteristics of fish habitats in agricultural waterways and suggests the need to manage them and study further.

• Korean Journal of Weed Science
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• v.16 no.4
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• pp.259-263
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• 1996

Occurrence and distribution of weeds were surveyed in autumn vegetable crops(Chinese cabbage, radish, cucumber and Welsh onion) in 16 cities or counties of Chungnam area during October and November 1995. Thirty weed species were occurred, and Rorippa globosa, Alopecurus aequalis var. amurensis, Stellaria media, Portulaca oleracea, Digitaria sanguinalis and Chenopodium album var. centrorubrum showed higher importance values among them. S. media, P. oleracea and A, aequalis var. amurensis were dominant in northwest coastal area, R globosa, D. sanguinalis and S. media in southwest coastal area, R globosa, A. aegualis var. amurensis and D. sanguinalis in northern area, and R globosa, A. aequalis var. amurensis and P. oleracea in southern area. S. media, R globosa and P. oleracea were dominant in Chinese cabbage fields, R globosa, Amaranthus retroflexus and S. media in radish fields, A. retroflexus, S. media and D. sanguinalis in cucumber fields, and R. globosa and P. oleracea in Welsh onion fields.

• Journal of Plant Biology
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• v.28 no.2
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• pp.165-175
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• 1985

The natural forest vegetation of the Piagol, Mt. Chiri, was classified into five major communities, i.e. Quercus mongolica, Carpinus laxiflora, C. tschonoskii, Q. serrata and Fraxinus mandshurica community. C. tschonoskii, C. laxiflora and Q. mongolica characteristically were dominated in the range of 500∼700 m, 700∼900 m and 900∼1,400 m in altitude, respectively. Abies nephrolepis and Rhododendron schlippenbachii in the ridge of Nogodan-Nalnaribong, and F. mandshurica and Q. serrata in the Yongsuam ravine were found as the dominant species. The transition between C. tschonoskii and C. laxiflora communities was relatively wide. The boundary between F. mandshurica, in the conglomerated zone, and the other communities surrounding its community was fairly abrupt. In Q. mongolica community, the canopy was relatively opened and the oak young tree of undergrowth was fairly dense. In the community of C. laxiflora and C. tschonoskii, the species composed of their understory was rich while their coverage was lower than that of Q. mongolica community. Shannon's diversity index was decreased in order of C. tschonoskii, C. laxiflora and Q. mongolica community, while their Simpson's dominance index increased conversely. Judging by the DBH-class distribution showing the negative exponential curve, these communities were considered as the climax forest. In Polar Ordination used samples (quadrats), the vegetation of the Piagol was divided into five groups, which was coincided with five communities classified by Braun-Blanquet system. In the ordination, the first axis and the second axis may be considered soil conditions and thermal conditions, respectively. It seems that two different methods, phytosociological method in macro level and ordination techniques in micro level, are useful for the vegetation classification.

• Korean Journal of Weed Science
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• v.31 no.1
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• pp.41-48
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• 2011

This survey of weed population by different soil moisture with paddy-upland rotation was conducted to investigate information on weed flora and its ecology between two years. Weed species were assessed in April : 2009, 2010. Soil samples were taken from each study plot to assess the impact soil moisture on the occurrence and abundance of dominance weed species. Soil water of poorly drained field ranged from 10.2~18.2% more than 11.8~14.3% of somewhat poorly drained field. Weeds of fields composed of 19 species belonging to 12 families. Among 12 families, 6 weed species belonged to Compositae (31.6%) were the biggest family, Cruciferae were 2 species (10.5%), and Rubiaceae were 2 species (10.5%), respectively. Youngia japonica had the highest summed dominance ratio(SDR) (97.0%) and followed by Conyza canadensis (92.1%), Galium spurium (35.3%) and Hemistepta lyrata (28.4%) of somewhat poorly drainage in 2009. Artemisia princeps the highest SDR (100%) and followed by Stellaria alsine(55.2%), Y. japonica (38.3%) and Nasturtium officinale (28.5%) of poorly drainage in 2009. And, Stellaria alsine had the highest SDR (86.8%) and followed by Alopecurus aequalis (77.7%), Astragalus sinicus (68.7%) and Y. japonica (46.3%) of somewhat poorly drainage in 2010. S. alsine the highest SDR (93.7%) and followed by A. aequalis (78.6%), Nasturtium officinale (31.3%) and Y. japonica (30.4%) of poorly drainage in 2010. Simpson's index was calculated to 0.12~0.23, which showed that weed occurrence with different soil moisture in paddy-upland rotation and between years was various. Similarity coefficient between years was 43.0% (2009) and 74.2% (2010), which indicate a low diversity because of the moisture in the agro-ecosystem.

• Journal of Korean Society of Forest Science
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• v.80 no.1
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• pp.54-71
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• 1991

To classify and analyze the forest communities and their structures, the vegetation in Mt. Joghe was investigated from July, 1980 to August, 1989. The results obtained are as follow ; 1. A total of 750 kinds of vascular plant(49 orders, 122 families, 434 genera, 627 species, 1 subspecies, 111 varieties and 11 forma)were observed in Mt. Ioghe. The newly observed plant species were Dioscorea quingueloba, Spiranthes sinensis, Cephalanthera falcata, Angelica gigas, Clematis patents, Paeonia obovata, Hibiscus mulabilis, Ainsliaea acerifolia, Dictamnus dasycarpus, Cynachum ascyrifolia, Vaccinium koreanum, Erythrortium japonicum, Indigofera kirilowii (17species), Broussonetia kazinoki var, humillis, Euonymus, fortunei var. radicans, Juniperus communis var, nippnnica, Callicarpa japonica var. radicans, Joniperus communis var. rzipponica, Callicarpa japonica var. taquetii (4 varieties) and L indera obtusiloba for. billosum (1 forma). 2. The life spectrum of flora in Mt. Joghe was classified into $CH-D_1-R_5-e$ type. Distribution area was identical to Southern type by Nakai, Lee, and Yim. A few subtropical species were also observed. 3. Simpson's species diversity index(Ds) was 0.9 and Shannon-Weiner's diversity index (H') was 1.004. These indice suggest that the vegetation in Mt. Joghe is of complicated forest communities. 4. Pte-Q was 1.81 which was higher than the nationwide mean of 1.68. Urbanization Index (UI) was 28.75 for naturalized plant species, and 17.49 for exotic woody plant species, which were similar to those of Mt. Baekun and Mt. Naejang. 5. The forest vegetation of Mt. Joghe was grouped in 3 vegetation types : 7 natural plant Communities dominated by Quercus serrat, Quercus acutissima, Quercus variabilis, Carpinus laxiflora, Pinus derasiflora and Platycarya strobilacea, 8 substitutional plant communities Styrax japonica, Stewartia koreana, Lindera erytlrrocarpa, Zelkova serrata, Rhtrs chinensis, Controversa, and Frzrxirtus manrlshurica, and 7 plantation Communities composed of Pinus koraiensis, Pinus rigida, Magnolia nbnvata, Chamecyparis obkrsa, Larie ieptolepis, Castanea crenata and Cryptomeria japonica. 6. Actual vegetation maps and profile diagrams were made by phytosocialogical classification. 7. As the important and unique species in Mt. Joghe, Lindera sericea, Penicaria tilitorme, Hex macropoda, Hex macropoda for. pseudo-macropoda, Steroartia koreana, Adenopkora palustris and Corylop.,is coreana, which were also seported by Lee(1977), Kim and Yark(1989), were identified and Vaccinium coreanum, Cremastra appendiculinium, Juniperus comminis van. nipponica, Cephalanthera falcata, Broussortetia kazinoki var. humilis, paeonia obovata, Deutzia prunifolia, Dictamnus dasyarpus, Angelica gigics and Bupleurum falcatum were odditionally observed.