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Development of Sequence-Based DNA Markers for Evaluation of Phylogenetic Relationships in Korean Watermelon Varieties

  • Lee, Hee-Jeong;Cho, Hwa-Jin;Lee, Kyung-Ah;Lee, Min-Seon;Shin, Yoon-Seob;Harn, Chee-Hark;Yang, Seung-Gyun;Nahm, Seok-Hyeon
    • Journal of Crop Science and Biotechnology
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    • v.10 no.2
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    • pp.98-105
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    • 2007
  • Phylogenetic relationships in Korean watermelons were evaluated by genetic similarity coefficients using 15 SSR(simple sequence repeat), 14 SCAR(sequence characterized amplified region) and 14 CAPS(sequence characterized amplified region) markers. The SSR markers were selected from previously reported melon and watermelon SSRs through testing polymorphisms within a set of commercial $F_1$ varieties. The SCAR and CAPS markers were developed from polymorphic AFLP(amplified fragment length polymorphism) markers between inbred lines 'BN4001' and 'BN4002'. From the AFLP analysis, 105 polymorphic fragments were identified between the inbred lines using 1,440 primer combinations of EcoRI+CNNN and XbaI+ANNN. Based on the sequencing data of these polymorphic fragments, we synthesized sequence specific primer pairs and detected clear and reliable polymorphisms in 27 primer pairs by indels(insertion/deletion) or RFLP(restriction fragment length polymorphism). A total of 43 sequence-based PCR markers were obtained and polymorphic information content(PIC) was analyzed to measure the informativeness of each marker in watermelon varieties. The average PIC value of SCAR markers was 0.41, which was similar to that of SSR markers. Genetic diversity was also estimated by using these markers to assess the phylogenetic relationships among commercial varieties of watermelon. These markers differentiated 26 Korean watermelon varieties into two major phylogenetic groups, but this grouping was not significantly correlated with their morphological and physiological characteristics. The mean genetic similarity was 66% within the complete set of 26 commercial varieties. In addition, these sequence-based PCR markers were reliable and useful to identify cultivars and genotypes of watermelon.

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Mineralogy of gold-silver deposits in Chungcheong Province (충청도(忠淸道) 일원(一圓)의 금(金)·은(銀)광상(鑛床)에 대한 광물학적(鑛物學的) 연구(硏究))

  • Choi, Seon Gyu;Park, No Young;Hong, Sei Sun
    • Economic and Environmental Geology
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    • v.21 no.3
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    • pp.223-234
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    • 1988
  • A large number of gold and/or silver-bearing quartz veins occur in or near Mesozoic granite batholith elongated in a NE-SW direction within the Chungcheong Province. Precambrian schists and gneisses, and Jurassic and Cretaceous granitic rocks serve as hosts for gold and/or silver deposits. On the basis of Ag/Au total production and ore grade ratio, 15 mines may be divided into three major groups: gold-dominant deposits, gold-silver deposits, and silver-dominant deposits. The chemical composition of electrum from skarn deposit (Geodo mine), alaskite-type deposit (Geumjeong mine) and 15 vein deposits was summarized. It was found that the Au content of electrum for vein deposits ranging from 5.2 to 86.5 is lower than that for skarn and alaskite deposits. Among 15 vein deposits, the composition of electrum associated with pyrrhotite is relatively high and has a narrow range of 40.8 to 86.5 atomic % Au, but the Au content of electrum with pyrite is in range of 5.2 to 82.8 atomic %, and is clearly lower than that with pyrrhotite. The grouping of ages for these mines indicates that gold and/or silver mineralizations occurred during two periods in the Mesozoic. Daebo igneous activities are restricted to gold mineralization in the range of 158 to 133 Ma, whereas Bulgugsa igneous activities are related to gold and/or silver mineralization ranging from 108 to 71 Ma. Generally speaking, Jurassic gold-dominant veins have many common characteristics; notably prominent association with pegmatites, simply massive vein morphology, high fineness in the ore concentrates, rarity of silver minerals, and a distinctively simple mineralogy, including sphalerite, galena, chalcopyrite, pyrrhotite and/or pyrite. Although individual deposits exhibit widely differing diversity, Cretaceous gold-silver and silver-dominant veins are characterized by features such as complex vein, low to medium fineness in the ore concentrates and abundance of silver minerals including Ag sulfosalts, Ag sulfides, Ag tellurides and native silver.

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Numerical taxonomy of Rhus sensu lato (Anacardiaceae) in Korea (한국산 광의의 붉나무속(Rhus L. sensu lato)의 수리분류학적 연구)

  • Tho, Jae-Hwa;Kim, Joo-Hwan
    • Korean Journal of Plant Taxonomy
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    • v.34 no.3
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    • pp.205-220
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    • 2004
  • Numerical analysis based on the 67 morphological characters from 28 populations of 6 species of Korean Rhus sensu lato (Anacardiaceae) was performed for the taxonomic delimitation. Based on the results of PCA with 47 quantitative characters, the sum of contributions for the total variance of three major principal components was 77,9% (PCl 35.2%, PC2 22.5% and PC3 20.2%). The sum of contributions for the total variance of three major principal components were 90,7% (PCl 37.7%, PC2 33.0% and PC3 20.0%) based on the results of PCA with 20 qualitative The characters. Two dimensional plotting from PCA results recognized six distinct species. UPGMA phenogram based on simple matching coefficient method recognized clear taxonomic delimitations among six taxa. On the cluster analysis, qualitative characters were more useful for grouping the species treated. Numerical analysis was very valuable to delimit the Korean taxa of Rhus s.l.

Hunminjungum Keypad (훈민정음 글자판)

  • Kim, Sungwook
    • Journal of Internet Computing and Services
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    • v.22 no.4
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    • pp.29-49
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    • 2021
  • This paper proposes the Hunminjungum Keypad that applied the creation principle of Hunminjungum to the design of keypad. The proposed keypad arranged 28 letters of Hunminjungum to have correlations with each other between consonants, between vowels, and between consonants and vowels. That is, Consonant buttons are arranged by grouping letters of the same sound by sounds of five voices. And the vowel buttons are arranged at the bottom and the right side of the consonant area according to the position where a vowel is attached to the consonant. In the meantime, Hangul keypads have mainly used 12 button keypads in 4 lines and 3 columns. These keypads have structurally disadvantageous in the touch count and moving distance. Recently, keypads with many letter buttons such as QWERTY and single-vowel are also used a lot. If the number of letter buttons provided in the keypad increases, touch count decreases. And If the letter buttons are arranged to have a correlation with each other, the moving distance becomes smaller. The experimental results show that the proposed keypad has high efficiency in all evaluation factors such as touch count, moving distance and input time.

An Experimental Study on AutoEncoder to Detect Botnet Traffic Using NetFlow-Timewindow Scheme: Revisited (넷플로우-타임윈도우 기반 봇넷 검출을 위한 오토엔코더 실험적 재고찰)

  • Koohong Kang
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.33 no.4
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    • pp.687-697
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    • 2023
  • Botnets, whose attack patterns are becoming more sophisticated and diverse, are recognized as one of the most serious cybersecurity threats today. This paper revisits the experimental results of botnet detection using autoencoder, a semi-supervised deep learning model, for UGR and CTU-13 data sets. To prepare the input vectors of autoencoder, we create data points by grouping the NetFlow records into sliding windows based on source IP address and aggregating them to form features. In particular, we discover a simple power-law; that is the number of data points that have some flow-degree is proportional to the number of NetFlow records aggregated in them. Moreover, we show that our power-law fits the real data very well resulting in correlation coefficients of 97% or higher. We also show that this power-law has an impact on the learning of autoencoder and, as a result, influences the performance of botnet detection. Furthermore, we evaluate the performance of autoencoder using the area under the Receiver Operating Characteristic (ROC) curve.

Design of an Enhanced Group Keypad to Prevent Shoulder-Surfing Attacks and Enable User Convenience (어깨 너머 공격을 차단하고 사용 편의성이 가능한 개선된 그룹 키패드 설계)

  • Hyung-Jin Mun
    • Journal of Practical Engineering Education
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    • v.15 no.3
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    • pp.641-647
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    • 2023
  • In the fintech environment, ensuring secure financial transactions with smartphones requires authenticating the device owner. Smartphone authentication techniques encompass a variety of approaches, such as passwords, biometrics, SMS authentication, and more. Among these, password-based authentication is commonly used and highly convenient for user authentication. Although it is a simple authentication mechanism, it is susceptible to eavesdropping and keylogging attacks, alongside other threats. Security keypads have been proposed to address vulnerabilities in password input on smartphones. One such innovation is a group keypad, resistant to attacks that guess characters based on touch location. However, improvements are needed for user convenience. In this study, we aim to propose a method that enhances convenience while being resistant to eavesdropping and recording attacks on the existing group keypad. The proposed method uses new signs to allow users to verify instead of the last character confirmation easily and employs dragging-to-touch for blocking recording attacks. We suggest diverse positioning methods tailored for domestic users, improving efficiency and security in password input compared to existing methods.

Genetic Diversity of Finger Millet (Eleusine coracana (L.) Gaertn.) Landraces Based on EST-SSR

  • Myung Chul Lee;Yu-Mi Choi;Myoung-Jae Shin;Hyemyeong Yoon;Seong-Hoon Kim
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.46-46
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    • 2020
  • Finger millet is more nutritious than other and millets and widely cultivate in tropical regions of the world. Furthermore, it is more tolerant against biotic and abiotic stresses such as pest, drought and salt. For this reason, finger millet is one of the putative crops to introduce and cultivate on reclaimed land and prepare the global climate exchange in Korea. In present study, genetic diversity and structure of different populations of finger millet from Africa and South Asia was examined at molecular level using newly developed EST-Simple Sequence Repeat (EST-SSR) markers. In total, 46 primers produced 292 alleles in a size range of 100-500 bp and mean Polymorphism Information Content (PIC) and Marker Index (MI) were 0.372 and 1.04, respectively. 46 primers showed polymorphism and 21 primers were identified as having a PIC value above 0.5. Principal coordinates analysis and the dendrogram constructed out of combined data of both markers showed grouping of finger millet accessions to their respective area of collection. The 156 accessions were more classified into four groups, such as three groups of Africa collection and one group of Asia. Results of present study can be useful in identifying diverse accessions and management of this plant resource. Moreover, the novel SSR markers developed can be utilized for various genetic analyses in this species in future.

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Effect of Diet Regimen of Sasang Constitution on Health Status (사상체질별 식이 섭생이 건강에 미치는 영향 -한방건강증진센터 시범운영을 위한 기초연구-)

  • Kim, Kwuy-Bun;Cho, Kyoul-Ja;Lee, Hyang-Yeon;Shin, Hye-Sook;Kim, Kwang-Joo;Moon, Heui-Ja;Kim, Yoon-Hee;Kang, Hyun-Sook;Park, Shin-Ae;Ji, Eun-Sun
    • Journal of East-West Nursing Research
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    • v.7 no.1
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    • pp.18-31
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    • 2002
  • This study, as a basic research to manage a Chinese Medicine Health Promotion Center by way of showing an example, is a before and after experiment research for simple group to verify a difference with cholesterol, health status and perception of health in order to confirm a effectiveness of diet and regimen according to the 4th status of physical constitution. Research object was chosen of 42 persons who operate a physical constitutional dietary regimen among them after selecting professors and clinical nurses (55 persons) majoring in the science of nursing who participated in Chinese Medicine-oriented Nurse Training Course from Aug. of 2001 to Feb. of 2002 all over the country. Diagnostic tools for physical constitution was used of the questionary that is currently consisted of physical constitution grouping test in Eastern & Western Diagnose Center of K Medical Center, and rating of health status was used of the tool that standardized CMI(Cornell Medical Index) to be available for Korean, and perception measurement for health status was used of a visual analogue scale for the health status that each one perceive personally, and physiological status was measured of cholesterol in blood. Analysis for the collected data was carried out by percentage, $X^2$ test, paired t-test according to research object by using SPSS, and the results of this study are as follows. 1) There was no difference with cholesterol before or after the experiment for objects. As a result of estimation about difference with health status by areas before or after the experiment, there are more improved result in eyes, ears, digestive organs, bones and sinews organs, frequency of a disorder, habit, adaptation status, angry, healthy status than before the experiment. As a whole, after the experiment the health was more improved than before the experiment. As the result to inspect a difference of health perception between before and after experiment, after the experiment the health perception level was improved than before, however there was no meaningful differences. 2) As the result to inspect a difference of cholesterol between before and after experiment according to object's physical constitution, in the case of So-yang-in(a person with the minimum male: according to the male and female principles(the sun and the moon)) among the 4th status of physical constitution there was only meaningful difference statistically, however, after the experiment their cholesterol's value was increased. As the result to inspect the difference of health status between before and after the experiment according to physical constitution, all of Ta-um-in(a person with maximum the female), So-yang-in(with the minimum male), So-um-in(with the minimum female) had a meaningful difference before and after the experiment, which means that in all case by physical constitutional groups, after the experiment their health status was more improved than before the experiment. As the result to inspect a difference of health perception between before and after according to physical constitutions, in the case of Tae-um-in and So-um-in, average score after the experiment was risen than before the experiment so that it means that the level of health perception was improved, however, there was no meaning statistically. According to the above results, if continuous diet and regimen by each physical constitutions could be implemented, it is certain that the health could be maintained and promoted. And, what we are healthy is for oneself to feel it subjectively. However, I think that cholesterol score in blood that we can view objectively could be changed distinctly if we can implement a strict diet and regimen. Accordingly, it is necessary for a method and period of experiment to be more strict and longer. According to the above results, I would like to suggest as follows. 1) In order to understand health status by Korean's physical constitutions and to generalize it, these research will be repeated against much more objects that could be selected by proper grouping method to consider a representative. 2) It is necessary for a research to inspect health status by physical constitution by developing a health status measurement tool that has higher confidence and propriety based on physical constitutional theory.

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Representative Physical and Chemical Properties of Korean Soils by the Results from Detailed Soil Survey (우리나라 토양(土壤)의 대표적(代表的)인 물리화학적(物理化學的) 특성(特性) (정밀토양조사결과(精密土壤調査結果)를 중심(中心)으로))

  • Hur, Bong-Koo;Jo, In-Sang;Min, Kyeong-Beom;Um, Ki-Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.17 no.4
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    • pp.330-336
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    • 1984
  • These studies were aimed to find out the representative values of physico-chemical properties in Korean soils and to serve the basic informations to improve the soil properties for increasing the soil productivity. By the results of detailed soil survey, 14 physico-chemical properties (soil texture, moisture characteristics, organic matter content etc) were collected from 315 series of soils except Cheju soils, computerized the simple mean and the distributed area by weighted mean values with grouping of land use, drainage class and soil type. The results were as follows; 1. Simple mean values within the efficient soil depth in whole country soils were clay 20.0%, organic matter 2.03% and CEC 10.3me/100g. But weighted mean values with land area belonged to the same soil series were clay 18.0%, OM 1.85% and CEC 8.6me/100g. 2. Mean values of paddy soils weighted by area were clay 19.7%, OM 2.0% and CEC 9.1me/100g but those of upland soils were 17.9%, 1.8% and 8.1me/100g and forest soils were 16.7%, 1.8% and 8.6me/100g respectively. 3. Weighted mean values of moderately well soils with covered area were clay 18.9%, organic matter 1.7%, CEC 8.4me/100g but those of imperfectly drained soils were 21.3%, 2.2% and 9.5me/100g, and those of poorly drained soils were 15.1%, 1.8% and 9.9me/100g respectivcly. 4. Simple mean and area weighted mean values of clay content, pH, organic matter contents, cation exchange capacity and base saturation were calculated by the types of paddy and upland.

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Genetic Diversity and Population Structure of Korean Mint Agastache rugosa (Fisch & Meyer) Kuntze (Lamiaceae) Using ISSR Markers

  • Kang, Man Jung;Sundan, Suresh;Lee, Gi An;Ko, Ho Cheol;Chung, Jong Wook;Huh, Yun Chan;Gwag, Jae Gyun;Oh, Se Jong;Kim, Yeon Gyu;Cho, Gyu Taek
    • Korean Journal of Plant Resources
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    • v.26 no.3
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    • pp.362-369
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    • 2013
  • Agastache rugosa, a member of the mint family (Labiatae), is a perennial herb widely distributed in East Asian countries. It is used in traditional medicine for the treatment of cholera, vomiting, and miasma. This study assessed the genetic diversity and population structures on 65 accessions of Korean mint A. rugosa germplasm based on inter simple sequence repeat (ISSR) markers. The selected nine ISSR primers produced reproducible polymorphic banding patterns. In total, 126 bands were scored; 119 (94.4%) were polymorphic. The number of bands generated per primer varied from 7 to 18. A minimum of seven bands was generated by primer 874, while a maximum of 18 bands was generated by the primer 844. Six primers (815, 826, 835, 844, 868, and 874) generated 100% polymorphic bands. This was supported by other parameters such as total gene diversity ($H_T$) values, which ranged from 0.112 to 0.330 with a mean of 0.218. The effective number of alleles ($N_E$) ranged from 1.174 to 1.486 with a mean value of 1.351. Nei's genetic diversity (H) mean value was 0.218, and Shannon's information index (I) mean value was 0.343. The high values for total gene diversity, effective number of alleles, Nei's genetic diversity, and Shannon's information index indicated substantial variations within the population. Cluster analysis showed characteristic grouping, which is not in accordance with their geographical affiliation. The implications of the results of this study in developing a strategy for the conservation and breeding of A. rugosa and other medicinal plant germplasm are discussed.