• Fisheries and Aquatic Sciences
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• v.25 no.10
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• pp.525-536
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• 2022

Photosynthetic bacteria (PSB) attract considerable interest as useful microorganisms; nevertheless, a generalized culture technique has not been previously reported owing to difficulty in their cultivation. Therefore, a simple culture technique suitable for public use was investigated. Among the PSB tested, the strain Rhodobacter azotoformans EBN-7 was the most suitable for scale-up production because it showed the highest specific growth rate (0.20 h^-1) on basal medium. In scale-up cultivation (500 L), R. azotoformans EBN-7 showed 4.50 × 10¹⁰ colony-forming units mL^-1 (number of viable cells), dry cell weight of 26.8 g/L, and a specific growth rate of 0.15 h^-1. Cultivation using this final culture broth (as seed culture) in a 15 L simple reactor was successful, with maintenance of cell activity evident. For use as seed culture, the maximum allowable preservation period of R. azotoformans EBN-7 at 4℃ was 3 months. When R. azotoformans EBN-7 cultivated in a simple technique was applied to shrimp aquaculture water, NH₄⁺-N was reduced from 0.61 mg/L to 0.24 mg/L (by 60.7%) in 4 days in comparison with the control. Thus, this simple culture technique using R. azotoformans EBN-7 has the potential for a good removal efficiency of NH₄⁺-N, making seed culture easier and suitable for public use.

• Parasites, Hosts and Diseases
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• v.51 no.6
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• pp.743-745
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• 2013

We compared the efficacy and applicability of a modified formalin-ether concentration technique (M-FECT) to the conventional FECT (C-FECT) and the agar plate culture (APC) method for the detection of Strongyloides stercoralis larvae. For this purpose, we used 600 human fecal specimens collected in an endemic area of southern Thailand. In the M-FECT, we used 2 layers of wire meshes, instead of gauze, to avoid the loss by absorption/adhesion of larvae to the gauze during filtration, and we reduced the exposure time of S. stercoralis larvae in stool samples to formalin. By such simple modifications, the efficacy of M-FECT has become comparable to APC and was much better than that of C-FECT for the diagnosis of strongyloidiasis.

• Korean Journal of Animal Reproduction
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• v.7 no.2
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• pp.41-52
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• 1983

Animal in dustry in Korea urgently needs the domestic introduction and the industrial a, pp.ication of embryo transfer technique. Namely, this technique can be utilized effectively, as means of the improvement of livestocks, as means of the increase of meat production, as means of substitute for the livestock import, and dissemination of new breed. However, as this technique avaliable in our country is remaining initial stage, we can not make use of the technique industrially unless we make much improvement as follows; induction of superovulation, non-surgical recovery of embryos, synchronization between the estrus such cycles of donor and recipient, non-surgical transfer of embryos, etc. Simultaneously, the basic studies such as harvesting oocytes from ovary, in vitro culture of oocytes, in vitro capacitation of spermatozoa, cloning by culture of blastomeres and transfer of nuclei, sexing embryo, etc. should not be neglected in order to make the technique of embryo transfer more simple and convenient. For the success of these studies, universities, national and public institutes, large scale cattle farms, and small scale cattle farms should cooperate each other. For instance, universities undertake basic researches, and the national and public institutes a, pp.y the results of the researches to animal industry along with cooperation by large scale cattle farms. By the help of the cooperative organizations, the technique relevant to our environment and farm condition may be able to be finalized, and to be a, pp.ied to samll scale cattle farm. Consequently, being served to stimulate animal productivity, this technique can be contributed to the development of livestock industry in Korea.

• The Plant Pathology Journal
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• v.30 no.4
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• pp.437-439
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• 2014

A fungus Pyrenophora tritici-repentis induces tan spot of wheat which is a foliar disease that causes yield loss to wheat crops worldwide. In this study, a new, simple and non-costly technique was performed to produce the sexual stage of this fungus in culture, within 9 weeks using wheat straw. This protocol will be helpful to researchers studying the biology of sexual stage development, disease epidemiology and genetics of this fungus.

• Korean Journal of Animal Reproduction
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• v.4 no.1
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• pp.75-82
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• 1980

This experiment was carried out to improve a simple and effective procedure of egg transfer which is considered to be the most useful technique for the improvementand proliferation of domestic animals. Several experiment procedures such as superovulation, surgical recovery of ovulated egg, in vitro capacitation of ejaculated spermatozoa, in vitro fertilization and culture of embryo were conducted. The results obtained in this experiment were summarized as follows: 1. In rabbits treated with PMS in combination with estradiol and HCG, 10 to 43 eggs were obtained in one rabbit and the average ovulation number of 20 rabbits was 21 eggs. 2. Six to 24 eggs (average 13 eggs) were recovered from the removed oviducts by the methods of flushing technique and the average recovery rate of 20 rabbits was 61.9 percent. 3. Most rabbit spermatozoa ejaculated by artificial vagina were capacitated in vitro by the culture of the spermatozoa with PBI medium at 37$^{\circ}C$ for 4 hours after removal of seminal plasma. 4. Normal fetilization following in vitro fetilization was observed in 88 (42.7%) of 206 eggs. 5. The number of eggs developed to 2-, 4-, and 8-cell stage following in vitro culture with PBI medium at 37$^{\circ}C$ was 26 (70.3%), 20 (54.1%) and 17 (45.9%) of 37 eggs used for in vitro culture.

• The Journal of Asian Finance, Economics and Business
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• v.8 no.5
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• pp.277-285
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• 2021

This study aims to examine the effect of variables of financial management information systems, organizational culture, and human resource competence on the accountability of village government financial reporting. The sample was 65 villages in the two districts using a simple random sampling technique. To analyze the data of this research, multiple regression analysis was conducted. The results showed that organizational culture as a differentiator in the two districts, namely financial management information systems, organizational culture, and human resource competence has a significant positive effect on accountability in preparing village government financial reports in Tabanan Regency. Meanwhile, organizational culture does not have a significant influence in Badung Regency, this is because each village government has a different work culture. In the Tabanan regency, we use local culture as the basis for organizational culture, while in the Badung regency it can be examined between organizational culture and it is carried out differently, which indicates that no one organizational culture type is superior to other types. This means that all types can move in line with and hand-in-hand based on how and when organizational goals are to be realized.

• Journal of Microbiology
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• v.45 no.6
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• pp.593-596
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• 2007

In this study, we describe the development of a simple and efficient method for cell lysis via the insertion of a bacteriophage lambda lysis gene cluster into the pET22b expression vector in the following order; the T7 promoter, a gene for a target protein intended for production, Sam7 and R. This insertion of R and Sam7 into pET22b exerted no detrimental effects on cellular growth or the production of a target protein. The induction of the T7 promoter did not in itself result in the autolysis of cells in culture but the harvested cells were readily broken by freezing and thawing. We compared the efficiency of the cell lysis technique by freezing and thawing to that observed with sonication, and determined that both methods completely disintegrated the cells and released proteins into the solution. With our modification of pET22b, the lysis of cells became quite simple, efficient, and reliable. This strategy may prove useful for a broad variety of applications, particularly in experiments requiring extensive cell breakage, including library screening and culture condition exploration, in addition to protein purification.

• Journal of the Korean Society of Costume
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• v.58 no.9
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• pp.1-17
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• 2008

One of features of fashion in Joseon period is culture of overlapping of several clothes. This culture appeared with Hapbong(合縫:sewing of several clothes as one) at late Joseon period and developed into simpler and more practical fashion culture. Hapbong is mainly identified at men's official uniform such as Jaebok(祭服), Jobok(朝服), Kwanbok(官福), Gugunbok(具軍服), and women's formal dress such as Wonsam(圓衫) and Dangeui(唐衣) which formed several clothes get to be a set. Such fashion was designed to maintain dignity and power while simple to wear. And It has the trace of overlapping visually on the collar, sleeve, breast-tie and others. Hapbong is attributable to thoughts of practical science, post-toadyism, modernization, renovated the system of clothing, and advanced sewing technique.

• The Research Journal of the Costume Culture
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• v.12 no.6 s.53
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• pp.918-932
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• 2004

Fashion is a product of form expressing the socio-cultural mood and aesthetic value of an age. Owing to development of the mass media and diversification of arts under the influence of post-modernism in the 20th century, fashion has been more briskly expressed to be presented to the people. With such basic conceptions in mind, this study was aimed at reviewing the fashion photography as visual medium. To this end, visual narrative techniques were analyzed and thereupon, Philip-Lorca diCorcia's fashion photography was examined. This study reviews such visual narrative techniques by dividing into visual and non-visual elements and thereby, examines the three-fold aspects of fashion photography expressions : Representative Level, Ground Level and Context Level. Also this study focuses on Philip-Lorca diCorcia's fashion photography in 'W magazine' which features portfolio form. This study analyzed the visual narratives shown in fashion photography and thereby, expand in diverse ways the fixed images of the conventional fashion photography commercials featuring beautiful models and exquisite costumes, and additionally, determined that fashion photography must be a kind of expression tool which could express not only the simple fashion but also the socio-psychological elements inherent in fashion.

• International Journal of Advanced Culture Technology
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• v.9 no.4
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• pp.288-294
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• 2021

There are various ways to be infected with malicious code due to the increase in Internet use, such as the web, affiliate programs, P2P, illegal software, DNS alteration of routers, word processor vulnerabilities, spam mail, and storage media. In addition, malicious codes are produced more easily than before through automatic generation programs due to evasion technology according to the advancement of production technology. In the past, the propagation speed of malicious code was slow, the infection route was limited, and the propagation technology had a simple structure, so there was enough time to study countermeasures. However, current malicious codes have become very intelligent by absorbing technologies such as concealment technology and self-transformation, causing problems such as distributed denial of service attacks (DDoS), spam sending and personal information theft. The existing malware detection technique, which is a signature detection technique, cannot respond when it encounters a malicious code whose attack pattern has been changed or a new type of malicious code. In addition, it is difficult to perform static analysis on malicious code to which code obfuscation, encryption, and packing techniques are applied to make malicious code analysis difficult. Therefore, in this paper, a method to detect malicious code through dynamic analysis and static analysis using Trojan-type Downloader/Dropper malicious code was showed, and suggested to malicious code detection and countermeasures.