• Journal of Korea Multimedia Society
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• v.12 no.3
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• pp.409-418
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• 2009

Providing Quality-of-Service (QoS) guarantee requires for each router on the path of a traffic flow not to violate the flow's delay budget allocated to itself. Since the amount of load being offered to the router is determined by the budget, some imbalance in load among routers on the path may be alleviated by means of adjusting the budget. The equal allocation applied to the resource reservation protocol (RSVP) is simple to implement, but it has the drawback of a poor resource utilization. A load balancing method in which the delay budget being allocated to a router depends on its load state was developed to improve the drawback, but it's too complex to apply to the RSVP. This paper proposes an intra-path load balancing method not only applicable to the RSVP but also more effective in improving the drawback. The proposed method first partitions the end-to-end delay bound of a flow to routers by the RSVP and then let them adjust their budgets according to both the bottleneck state of the path and their links' bandwidth availabilities. The results of the simulation applying the proposed method to an evaluation network showed that the proposed method may provide the gain of 4 ${\sim}$ 17 % compared to that in the legacy one in terms of the number of maximally admittable flows.

• The Korean Journal of Nuclear Medicine
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• v.28 no.1
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• pp.106-111
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• 1994

2-iminothiolane is known to bind $NH_2$ group of lysine in the protein and deliver SH group, which can be used to label protein with $^{99m}Tc$. In this study, we looked for the best reaction condition in which 2-iminothiolane is conjugated to human polyclonal IgG and labeling condition with $^{99m}Tc$-glucoheptonate. Labeling yield was measured with TSK G4000SW column and HPLC or precipitation with 10% TCA (trichloroacetic acid) and 1% HSA. In vivo distribution was investigated with Staphylococcal abscess bearing rats. With decreasing glucoheptonate, the labeling yield decreased. Without 2-iminothiolane, $^{99m}Tc$-glucoheptonate was bound to IgG, which seemed to be direct labeling. With increasing 2-iminothiolane upto 20 times higher than IgG, the labeling yield increased, and plateau was seen with higher molar excess of 2-iminothiolane. Polymer formation was not observed. The pH for the conjugation of 2-iminothiolane and IgG was best around 6.4. $^{99m}Tc$-2-iminothiolane-IgG showed faster blood clearance, higher renal activity and lower hepatic and splenic activity than $^{99m}Tc$-DTPA-IgG. The biodistribution of $^{99m}Tc$-2-iminothiolane-IgG with higher molar excess of 2-iminothiolane was not different from that with lower molar excess. Labeling antibodies with $^{99m}Tc$ using 2-iminothiolane can afford a possible route to simple labeling and wide clinical use of the immunoscintigraphy.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.1
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• pp.47-52
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• 1985

A radioimmunoassay technique has been developed for the quantitative analysis of Abscisic acid (ABA). The antibody, obtained by immunizing rabbits against a conjugate of ABA with human serum albumin, had a high affinity (Ka=3.28x10$\^$13/l/mol) for ABA. The use of $^3$H-labelled ABA as tracer and of dextran-coated charcoal for separation of free ABA from antibody-bound ABA permitted detection of as little as 0.5x10$\^$-12/ mol ABA. The measuring range extended to 14x10$\^$-12/ mol. Because of the high specificity of this immunoassay, no extract purification steps were required prior to analysis. And then, only 2 hr in radioimmunoassay was required to ABA analysis. From these results, it is suggested using this assays that more than hundreds samples can be assayed sensitive and simple per day for ABA.

• Journal of Communications and Networks
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• v.13 no.6
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• pp.602-611
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• 2011

The inter-domain routing scalability issue is a major challenge facing the Internet. Recent wide deployments of multihoming and traffic engineering urge for solutions to this issue. So far, tunnel-based proposals and compact routing schemes have been suggested. An implicit assumption in the routing community is that structured address labels are crucial for routing scalability. This paper first systematically examines the properties of identifiers and address labels and their functional differences. It develops a simple Internet routing model and shows that a binary relation T defined on the address label set A determines the cardinality of the compact label set L. Furthermore, it is shown that routing schemes based on flat address labels are not scalable. This implies that routing scalability and routing stability are inherently related and must be considered together when a routing scheme is evaluated. Furthermore, a metric is defined to measure the efficiency of the address label coding. Simulations show that given a 3000-autonomous system (AS) topology, the required length of address labels in compact routing schemes is only 9.12 bits while the required length is 10.64 bits for the Internet protocol (IP) upper bound case. Simulations also show that the ${\alpha}$ values of the compact routing and IP routing schemes are 0.80 and 0.95, respectively, for a 3000-AS topology. This indicates that a compact routing scheme with necessary routing stability is desirable. It is also seen that using provider allocated IP addresses in multihomed stub ASs does not significantly reduce the global routing size of an IP routing system.

• Food Science of Animal Resources
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• v.33 no.4
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• pp.501-507
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• 2013

Lysozyme was trapped from $2{\times}$ diluted egg white using Amberlite FPC 3500 ion exchange resin (1 g/10mL of egg white). The lysozyme bound to the resin was recovered using 0.1 N glycine-NaOH buffers, pH 9.0, containing 0.5 M NaCl. After separating lysozyme, the pH of the egg white solution was adjusted to 4.75 and centrifuged to remove interfering proteins. The supernatant was collected, added with 2.5% citric acid and 5.0% ammonium sulfate combination to precipitate egg white proteins, except for ovalbumin. After centrifugation, both supernatant (S1) and precipitant were collected. The precipitant was dissolved with 4 volumes of distilled water, and then 2.0% ammonium sulfate and 1.5% citric acid combinations added, stirred overnight in a cold room, and centrifuged. The resulting supernatant (S2) was pooled with the first supernatant (S1), desalted using an ultrafiltration unit, heat-treated at $70^{\circ}C$ for 15 min, and then centrifuged. The supernatant was collected as an ovalbumin fraction and lyophilized. The separated proteins were confirmed using Western blotting. The yield of lysozyme and ovalbumin was > 88.9% and > 97.7%, respectively, and the purity of lysozyme and ovalbumin was > 97% and 87%, respectively. The results indicated that the protocol was simple, and separated lysozyme and ovalbumin effectively.

• JSTS:Journal of Semiconductor Technology and Science
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• v.7 no.3
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• pp.140-150
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• 2007

A simple, label-free microfluidic cell purification method is presented for separation of cancer cells by exploiting difference in cell adhesion. To maximize the adhesion difference, three types of polymeric nanostructures (50nm pillars, 50nm perpendicular and 50nm parallel lines with respect to the direction of flow) were fabricated using UV-assisted capillary moulding and included inside a polydimethylsiloxane (PDMS) microfluidic channel bonded onto glass substrate. The adhesion force of human breast epithelial cells (MCF10A) and human breast carcinoma (MCF7) was measured independently by injecting each cell line into the microfluidic device followed by culture for a period of time (e.g., one, two, and three hours). Then, the cells bound to the floor of a microfluidic channel were detached by increasing the flow rate of medium in a stepwise fashion. It was found that the adhesion force of MCF10A was always higher than that of MCF cells regardless of culture time and surface nanotopography at all flow rates, resulting in a label-free detection and separation of cancer cells. For the cell types used in our study, the optimum separation was found for 2 hours culture on 50nm parallel line pattern followed by flow-induced detachment at a flow rate of $300{\mu}l/min$.

• Proceedings of the KIEE Conference
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• 2006.10a
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• pp.53-54
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• 2006

Arsenic doped p-type ZnO thin films have been realized on intrinsic (100) GaAs substrate by RF magnetron sputtering and thermal annealing treatment. p-Type ZnO exhibits the hole concentration of $9.684{\times}10^{19}cm^3$, resistivity of $2.54{\times}10^{-3}{\Omega}cm$, and mobility of $25.37\;cm^2/Vs$. Photoluminescence (PL) spectra of As doped p-type ZnO thin films reveal neutral acceptor bound exciton ($A^{0}X$) of 3.3437 eV and a transition between free electrons and acceptor levels (FA) of 3.2924 eV. Calculated acceptor binding energy ($E_A$) is about 0.1455 eV. Thermal activation and doping mechanism of this film have been suggested by using X-ray photoelectron spectroscopy (XPS). p-Type formation mechanism of As doped ZnO thin film is more related to the complex model, namely, $As_{Zn}-2V_{Zn}$, in which the As substitutes on the Zn site, rather than simple model, Aso, in which the As substitutes on the O site. ZnO-based p-n junction was fabricated by the deposition of an undoped n-type ZnO layer on an As doped p-type ZnO layer.

• KSBB Journal
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• v.17 no.1
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• pp.20-25
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• 2002

We performed a basic experiment for rapid, on-line, real-time measurement of HBsAg by using a surface plasmon resonance biosensor to quantify the recognition and interaction of biomolecules. We immobilized the anti-HBsAg polyclonal antibody to the dextran layer on a CM5 chip surface which was pre-activated by N-hydroxysuccinimide for amine coupling. The binding of the HBsAg to the immobilized antibody was measured by the mass increase detected by the change in the SPR signal. The binding characteristics between HBsAg and its antibody followed typical monolayer adsorption isotherm. When the entire immobilized antibody was interacted, there was no additional, non-specific binding observed, which suggested the biointeraction was very specific as expected and independent of the ligand density. No significant steric hindrance was observed at 17.6 nm/$mm^2$ immobilization density. The relationship between the HBsAg concentration in the sample solution and the antigen bound to the chip surface was linear up to ca. $40\mu\textrm{g}$/mL, which is much wider than that of the ELISA method. It appeared the antigen-antibody binding was increased as the immobilized ligand density increased, but verification is warranted. This study showed the potential of this biosensor-based method as a rapid, simple, multi-sample, on-line assay. Once properly validated, it can serve as a more powerful method for HBsAg quantification replacing the current ELISA method.

• Structural Engineering and Mechanics
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• v.17 no.5
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• pp.691-706
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• 2004

The paper presents shear lag parameters for beam-to-column connections in steel box piers. Previous researches have analyzed beam-to-column connections in steel piers using a shear lag parameter ${\eta}_o$ obtained from a simple beam model, which is not based on a reasonable design assumption. Instead, the current paper proposes a cantilever beam model and has proved the effectiveness through theoretical and experimental studies. The paper examines the inaccuracy of the previous researches by estimating the effective width, the width-span length ratio L/b, and the sectional area ratio S of a cantilever beam. Two different shear lag parameters are defined using the cantilever model and the results are compared each other. The first type of shear lag parameter ${\eta}_c$ of a cantilever beam is derived using additional moments from various stress distribution functions while the other shear lag parameter ${\eta}_{eff}$ of a cantilever beam is defined based on the concept of the effective width. An evaluation method for shear lag stresses has been investigated by comparing analytical stresses with test results. Through the study, it could be observed that the shear lag parameter ${\eta}_{eff}$ agrees with ${\eta}_c$ obtained from the $2^{nd}$ order stress distribution function. Also, it could be observed that the shear lag parameter ${\eta}_c$ using the $4^{th}$ order stress distribution function almost converges to the upper bound of test results.

• Journal of the Korea Society of Computer and Information
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• v.17 no.12
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• pp.149-158
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• 2012

Proposed is a hybrid randomizing function using two asymptotically optimal randomizing functions: Elias function and Peres function. Randomizing function is an mathematical abstraction of producing a uniform random bits from a source of randomness with bias. It is known that the output rate of Elias function and Peres function approaches to the information-theoretic upper bound. Especially, for each fixed input length, Elias function is optimal. However, its computation is relatively complicated and depends on input lengths. On the contrary, Peres function is defined by a simple recursion. So its computation is much simpler, uniform over the input lengths, and runs on a small footprint. In view of this tradeoff between computational complexity and output efficiency, we propose a hybrid randomizing function that has strengths of the two randomizing functions and analyze it.