• Archives of Pharmacal Research
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• 제28권10호
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• pp.1147-1151
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• 2005

A new naturally occurring sterol, compound 5, and six known stigmasterols were isolated from fruits of Ailanthus altissima Swingle by repeated column chromatography and RP-HPLC. Their structures were identified as, 5${\alpha}$-stigmastane-3,6-dione (1), 3${\beta}$-hydroxystigmast-5-en-7-one (2), stigmast-5-ene-3${\beta}$, 7${\alpha}$-diol (3), 6${\alpha}$-hydroxystigmast-4-en-3-one (4), 5${\alpha}$-stigmastane-3${\beta}$, 6${\beta}$-diol (5), stigmast-4-ene-3${\beta}$, 6${\alpha}$-diol (6), stigmast-5-ene-3${\beta}$, 7${\alpha}$, 20$\xi$-triol (7) by spectral analysis and comparison with the published data. These compounds have not been reported from genus Ailanthus, whereas compound 7 was identified by NMR for the first time. In addition, the $95\%$ ethanol extract and compounds from the fruits of Ailanthus altissima SWINGLE were assayed for in vitro antimicrobial activity. The extract was potent active against the assayed bacteria while compounds 3 and 7 exhibited moderate activity.

• Bulletin of the Korean Chemical Society
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• 제32권1호
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• pp.127-130
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• 2011

One new tirucallane-type triterpenoid, alianthusaltinin A (1), one new $C_{29}$ sterone, alianthaltone A (2), and 12 known compounds have been isolated from the stem bark of Ailanthus altissima. The structures of new compounds were identified by means of spectroscopic methods. Compound 3 was isolated from natural sources for the first time, and compounds 4, 5, and 9 were isolated from this plant for the first time.

• 한국자원식물학회지
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• 제7권1호
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• pp.29-34
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• 1994

Two new 1,2-seco-1-nor-6((5 $\mapsto$ 10)abeo-picrasan-2,5-olideskeleton quassinoids, eurylactones A [1] and B [2], and two new $C_{19}$ skeleton quassinoids 3 and 4 were isolated from Eurycoma longifolia together with two known $C_{18}$ quassinoids 5 and 6. Their sttuctures were established by the spectral evidence.Many quassinoids have already been isolated (1-3) from the roots, wood, and leaves of Eurycoma longiolia Jack (Simaroubaceae) and have been used as a folk medicine insoutheast Asia. As part of a series of studies, we have further undertaken the chemicalinvestigation of the n-BuOH-soluble fraction of the MeOH extract and isolated two new1,2-seco-1-nor-6(5 $\mapsto$ 10)abeo-picrasane skeleton quassinoids 1 and 2, which have beenknown only in shinjulactone B (4,5) and yadanfiolide (6), two new $C_{19}$-type quasslnolds3 and 4, and two known C18-type quassinoids 5 and 6. In this paper, the structureelucidation and biosenetic relation of these compounds are described.

• 생약학회지
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• 제32권4호통권127호
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• pp.274-279
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• 2001

Ailanthus altissima belonging to the family Simaroubaceae has been used to settle an upset stomach, to combat a fever, and as an insecticide. Apoptosis is an active process, which is a critical feature of the regulated development of multicellular organisms. We investigated whether the extract of A. altissima induced apoptotic cell death in Jurkat T-acute lymphoblastic leukemia (ALL) cells. Upon treatments with the extract, the dose-dependent inhibitions of cell viability were observed. It also caused apoptosis as measured by cell morphology and DNA fragmentation. The capability of the extract to induce apoptosis was associated with proteolytic cleavage of specific target protein such as poly(ADP-ribose)polymerase (PARP) protein, suggesting the possible involvement of the activations of caspases. Further study showed that Bcl-2 protein levels were not changed in all treated groups compared to control group. These results suggest that A. altissima induces Bcl- 2-independent apoptosis in Jurkat T cells.

• Journal of Applied Biological Chemistry
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• 제56권4호
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• pp.213-217
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• 2013

Phytochemical studies on the leaves of Ailanthus altissima (Simaroubaceae) have not been reported previously. Thus, the authors isolated and identified secondary metabolites from A. altissima. Dried and powdered leaves were extracted with 80% aqueous methanol, and the concentrated extract was successively partitioned with ethyl acetate, n-butanol, and water. Four flavonoids were isolated from the ethyl acetate fraction through repeated silica gel and octadecyl silica gel column chromatography. Spectroscopic data including NMR, MS, and IR allowed for identification of the chemical structures as quercetin (1), afzelin (2), quercitrin (3), and isoquercitrin (4). This is the first report of the isolation of these compounds from A. altissima. The four isolated flavonoids 1-4 as well as solvent fractions (ethyl acetate, n-butanol, and water), were evaluated for DPPH radical scavenging activity.