• Title/Summary/Keyword: Silver transport

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The Mitochondrial Tricarboxylate Carrier of Silver Eel: Chemical Modification by Sulfhydryl Reagents

  • Capobianco, Loredana;Impagnatiello, Tecla;Ferramosca, Alessandra;Zara, Vincenzo
    • BMB Reports
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    • v.37 no.5
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    • pp.515-521
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    • 2004
  • The tricarboxylate (or citrate) carrier was purified from eel liver mitochondria and functionally reconstituted into liposomes. Incubation of the proteoliposomes with various sulfhydryl reagents led to inhibition of the reconstituted citrate transport activity. Preincubation of the proteoliposomes with reversible SH reagents, such as mercurials and methanethiosulfonates, protected the eel liver tricarboxylate carrier against inactivation by the irreversible reagent N-(1-pyrenyl)maleimide (PM). Citrate and L-malate, two substrates of the tricarboxylate carrier, protected the protein against inactivation by sulfhydryl reagents and decreased the fluorescent PM bound to the purified protein. These results suggest that the eel liver tricarboxylate carrier requires a single population of free cysteine(s) in order to manifest catalytic activity. The reactive cysteine(s) is most probably located at or near the substrate binding site of the carrier protein.

Effects of Silver lon Exchanged Water Treatment Agent upon E. Coli RB 797 and Bacillus sp. (수처리제 은이온이 E. Coli RB 797과 Bacillus sp. 에 미치는 영향)

  • 신혜자;신춘환
    • Journal of Life Science
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    • v.7 no.4
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    • pp.316-321
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    • 1997
  • The effects of the silver ion-exchanged water treatment agent (Ag-Os) upon E. coli RB 797 and Bacillus sp. have been discussed in this study. Silver ion causes a number of toxic effects with no known biological function. Silver ion-exchanged water treatment agent (Ag-Os) using oyster shell here showed antimicrobial activities. the soluble form of silver ion in water is more toxic to the growth of Bacillus sp. than that of E. Coli RB 797. The minium amount of Ag-Os needed for growth inhibition is 0.2 mg/ml for E. Coli RB 797 and 0.02 mg/ml for Bacillus sp., which is consistant with the data of the survival cell fraction. Binding studies suggested that binding of silver to the cell surface was a rapid, metabolic-independent process and different from active transport. Bacillus sp. showed more binding than E. Coli RB 797. Reducing substances of the cell cultures in the presence of Ag-Os was detected using Methylen blue as an indicator. From these results, we suggest that Ag-Os is effective as an antimicrobial agent on E. Coli RB 797 and Bacillus sp. and silver binds to the cells through rapid, metabolic-independent process and might complex to sulfur group in the cells for its toxicity.

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PEBAX-2533/Ag Salt/Al(NO3)3 Composite Using AgCF3SO3 for Facilitated Olefin Transport Membrane (올레핀 촉진수송 분리막용 AgCF3SO3를 활용한 PEBAX-2533/Ag Salt/Al(NO3)3 복합체)

  • Kim, Soyoung;Kang, Sang Wook
    • Membrane Journal
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    • v.31 no.1
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    • pp.61-66
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    • 2021
  • This study was a study on an facilitated transport membrane to replace the cryogenic separation method currently used in olefin/paraffin separation. Cost reduction is also a very important factor to commercialize facilitated transport membranes. However, AgBF4, which has been studied a lot, is a relatively expensive silver salt. To replace this, a PEBAX-2533/ AgCF3SO3/Al(NO3)3 composite film was prepared using relatively inexpensive AgCF3SO3. It was analyzed through SEM, FT-IR, and RAMAN. Through this study, it was confirmed that the polymer matrix affects the long-term stability.

Development and properties of jointed Bi-2223 superconductor tape

  • Kim, Jung-Ho;Ji, Bong-Ki;Park, Hyung-Sang;Kim, Ho-Jin;Oh, Seung-Jin;Kim, Joong-Seok;Joo, Jin-Ho;Nah, Won-Soo
    • 한국초전도학회:학술대회논문집
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    • v.10
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    • pp.298-303
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    • 2000
  • We evaluated the electric properties of Bi-2223 jointed tapes processed by both resistive- and supercondcuting-joint methods. For the resistive-joint, filler materials of wood metal, Pb/Sn, In, and silver paste were used, whereas, for the superconductive-joint, the lap joint method were used. In the resistive-joint tape, it was observed that the electrical properties such as current transport property, n-value, and contact resistance of the tape were significantly related to the resistivity of filler materials. On the other hand, in the superconducting-joint tape, the current transport property was dependent on the uniaxial pressure. Specifically, the current transport property varied 50 to 80% with uniaxial pressure, probably due to the irregular microstructure in the transition region.

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Utilization of Iodine for the Enhanced Permeance of Facilitated Olefin Transport Nanocomposite Membrane (올레핀 촉진수송 나노복합체 분리막의 투과도 향상을 위한 iodine의 활용)

  • Choi, Yeji;Lee, Eun Yong;Kang, Sang Wook
    • Membrane Journal
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    • v.24 no.6
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    • pp.448-452
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    • 2014
  • Nanocomposite membrane to show facilitated olefin transport was prepared for enhanced separation performance. Addtion of halogen molecules into PVP/AgNPs/ TCNQ nanocomposite membrane was expected to further polarize the surface of AgNPs for enhancing the separation performance. The formation of AgNPs and presence of iodine was confirmed by TEM and EDS analysis, respectively. The separation performance for propylene/propane mixture was compared with that of PVP/AgNPs/TCNQ nanocomposite membrane. The long-term stability of membrane was investigated with time.

Morphology, Mineralogy and Genetic Implication of Placer Gold from the Huongkhe Area, Vietnam (베트남 홍케 지역 사금의 산상과 생성연구)

  • Choi, Sang-Hoon;Choi, Seon-Gyu;Han, Jin-Kyun
    • Economic and Environmental Geology
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    • v.29 no.3
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    • pp.235-246
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    • 1996
  • Placer gold in collected heavy minerals from several localities in Huongkhe area, is consistently very finegrained (${\leq}100$ to $400{\mu}m$). The size and size distribution show somewhat differences at Dongdo and Hoahai : at Dongdo, predominant relatively larger and wide distribution; at Hoahai, characteristic relatively finer and narrow distribution range. The morphology of gold grains is divided into the four groups assumed by the dimension ratio : spherical, subprismoidal, prismoidal, and irregular. The gold grains at Dongdo show wide morphological distribution, whereas, at Hoahai, spherical form is predominant (${\approx}75%$). Three main types of gold are classified based on their chemical composition and mode of occurrence: type I (electrum; fineness=568~931), type II (amalgam; fineness=671~927), and type III (native gold; fineness=923~999). Type I gold contains, relatively high and variable silver contents (${\approx}11$ to 58 atomic % Ag), and has been classified into two subtypes based on their silver contents (type IA, ${\approx}11{\sim}39$ atomic % Ag; type IB, ${\approx}40{\sim}58$ atomic % Ag). However, type I gold would have been generally original compositions of electrum which originated at the provenance deposits. Mercury reacts with gold and silver to form amalgam (type II gold) which has variable Hg contents (1.2~30.5 atomic % Hg). The mercury contents in gold grains at Hoahai (10.9~30.5 atomic % Hg) are higher than those at Dongdo (5.8~21.1 atomic % Hg). The gold grains from the area generally exhibit a high-purity gold (type III) rim. The individual rims on the various grains range from <1 to $80{\mu}m$ in thickness and have silver contents of <10 atomic percent Ag, even though the core compositions range from ${\approx}11$ to 58 atomic percent Ag. The rim of gold most likely is responsible for the commonly cited cases of gold from placer deposits assaying at higher values of fineness than the gold in the corresponding source lode. The gold-rich rim in the Huongkhe area apparently forms by a combination of self-electrorefining and preferential dissolution of silver under oxidizing nature during the weathering and transport process. All data of gold grains in the Huongkhe area suggest that the transport distances and/or time of placer gold at Hoahai are generally farther than those at Dongdo. The mercurian gold bearing provenance deposits at Dongdo and Hoahai would be suggest nearest epithermal gold-silver vein-type.

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Light Scattering Effect of Incorporated PVP/Ag Nanoparticles on the Performance of Small-Molecule Organic Solar Cells

  • Heo, Il-Su;Park, Da-Som;Im, Sang-Gyu
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.08a
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    • pp.221-221
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    • 2012
  • Small-molecule organic photovoltaic cells have recently attracted growing attention due to their potential for the low-cost fabrication of flexible and lightweight solar modules. The PVP/Ag nanoparticles were synthesized by the reaction of poly vinylpyrrolidone (PVP) and silver nitrate at $150^{\circ}C$. In the reaction, the size of the nanoparticles was controlled by relative mole fractions between PVP and Ag. The PVP/Ag nanoparticles with various sizes were then spin coated on the patterned ITO glass prior to the deposition of the PEDOT:PSS hole transport layer. The scattering of the incident light caused by these incorporated nanoparticles resulted in an increase in the path length of the light through the active layer and hence the enhancement of the light absorption. This scattering effect increased as the size of the nanoparticles increased, but it was offset by the decrease in total transmittance caused by the non-transparent nanoparticles. As a result, the maximum power conversion efficiency, 0.96% which was the value enhanced by 14% compared to the cell without incorporation of nanoparticles, was obtained when the mole fraction of PVP:Ag was 24:1 and the size of the nanoparticles was 20~40 nm.

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Mobility of silver nanoparticles (AgNPs) and oxidative degradation of endocrine disrupting chemicals by saturated column experiments (포화컬럼실험에서 산화공정을 적용한 내분비계 장애물질의 제거 및 은나노물질의 거동 연구)

  • Kim, Yejin;Heo, Jiyong
    • Journal of Korean Society of Water and Wastewater
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    • v.32 no.6
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    • pp.499-505
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    • 2018
  • We applied column experiments to investigate the environmental fate and transport of silver nanoparticles(AgNPs) in fully saturated conditions of porous media. These column experiments were performed to emphasize oxidation method with $H_2O_2$ concentration and acidic conditions. The mobility of AgNPs was decreased with the increasing ionic strength that the surface charge of AgNPs(zeta potential) was neutralized with the presence of positive ions of $Na^+$. Additionally, it was also affected due to that not only more increased aggregated size of AgNPs and surface charge of quartz sand. The decreased breakthrough curves(BTCs) of bisphenol-A(BPA) and $17{\alpha}$-ethynylestradiol(EE2) were removed approximately 35.3 and 40%. This is due to that endocrine disrupting chemicals(EDCs) were removed with the release of $OH{\cdot}$ radicals by the fenton-like mechanisms from acidic and fenton-like reagent presenting. This results considered that higher input AgNPs with acidic conditions is proved to realistic in-situ oxidation method. Overall, it should be emphasized that a set of column experiments employed with adjusting pH and $H_2O_2$ concentration in proved to be effective method having potential ability of in-situ degradation for removing organic contaminants such as BPA and EE2.

Service Differentiation in Ad Hoc Networks by a Modified Backoff Algorithm (애드혹 네트워크 상에서 backoff 알고리즘 수정에 의한 서비스 차별화)

  • Seoung-Seok Kang;Jin Kim
    • Journal of KIISE:Information Networking
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    • v.31 no.4
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    • pp.414-428
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    • 2004
  • Many portable devices are coming to be commercially successful and provide useful services to mobile users. Mobile devices may request a variety of data types, including text and multimedia data, thanks to the rich content of the Internet. Different types of data and/or different classes of users may need to be treated with different qualities of service. The implementation of service differentiation in wireless networks is very difficult because of device mobility and wireless channel contention when the backoff algorithm is used to resolve contention. Modification of the t)mary exponential backoff algorithm is one possibility to allow the design of several classes of data traffic flows. We present a study of modifications to the backoff algorithm to support three classes of flows: sold, silver, and bronze. For example, the gold c]ass flows are the highest priority and should satisfy their required target bandwidth, whereas the silver class flows should receive reasonably high bandwidth compared to the bronze class flows. The mixture of the two different transport protocols, UDP and TCP, in ad hoc networks raises significant challenges when defining backoff algorithm modifications. Due to the different characteristics of UDP and TCP, different backoff algorithm modifications are applied to each class of packets from the two transport protocols. Nevertheless, we show by means of simulation that our approach of backoff algorithm modification clearly differentiates service between different flows of classes regardless of the type of transport protocol.