• Journal of Sericultural and Entomological Science
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• v.42 no.2
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• pp.109-113
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• 2000

The preparation of skin care gauze could be make to mixing separated cellulose from waste milk pack and degummed silk fibroin fiber. Also, its wound covering and anti-bacterial activity were investigated in order to find out the enhancement of their functionality. By the 30% silk fibroin fiber including skin care gauze, the anti-bacterial activity values of Staphylococcus strain are much 4 times higher than of 0∼10% silk fibroin fiber including skin care gauze. The average yield of cellulose from waste milk pack was obtained 50-60%, and their morphologies, physical properties, modulus and biodegradation ratios are studies, respectively.

• Journal of Sericultural and Entomological Science
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• v.39 no.2
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• pp.186-196
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• 1997

The IR crystallinity index of Calcium nitrate treated silk fiber decreased proportionally to the concentration of calcium nitrate. A partial change of conformation was observed in the concentration of over 46.4-47.6% changing from $\beta$-sheet or to random coil in the crystalline region. This is in coincidence with the result of crystallinity index, which was started to be reduced in the concentration range of 46.4-47.6%. A same trend was observed for the X-ray order factor, birefringence, degree of orientation and surface structure. These structural parameters were remarkably changed on the treatment of silk fibers with concentration of 46.4-17, 6% calcium nitrate. Therefore, it seems that there exists a critical concentration of calcium nitrate in affection the structure and morphology of silk fibers. According to the examination of surface morphology, the fine stripe was observed in the direction of fiber axis at 46.4% concentration. However, the treated concentration was exceeded by 47.6%, the cracks were appeared severely on the fiber surface in the transverse direction as well as fiber axis direction. This result might be related to the tensile properties, specially a tenacity of silk fibers. As a result of quantitative analysis of a dilute acid hydrolysis, three different regions, which are known as a amorphous, semi-crystalline and crystalline region, could be obtained. The hydrolysis rate curves were different with various concentrations of treatment and the relative contents of each region could be calculated.

• Fibers and Polymers
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• v.6 no.1
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• pp.1-5
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• 2005

In this study, we attempted to evaluate a novel use of sericin-fixed silk fiber (SFx) as an immobilization support of enzyme. Sericin was fixed on the silk fiber using glutaraldehyde as a fixation reagent. After 6 hours of fixation, the degree of fixation increases linearly with linear decrease of the amount of bound $\alpha$-chymotrypsin (CT). This suggests that the increase of the degree of fixation is due to the further crosslinking of free aldehyde groups on the surface of sericin-fixed silk fiber (SFx). Even though perfect fixation was not achieved, sericin did not dissolve seriously and could be removed by further washing. The specific activity did not differ significantly after 6 hours of fixation. The activity of immobilized CT on SFx decreased to its half after 6 hours of incubation at 50$^{\circ}C$. However, it retained $78\%$ of initial activity even after 1 hour of treat­ment with $100\%$ ethanol. As a result, the SFx could be used as an immobilization support of enzyme in non-aqueous media at ambient temperature.

• Composites Research
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• v.27 no.5
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• pp.196-200
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• 2014

A novel, simple and totally recyclable method has been developed for the synthesis of nontoxic, biocompatible and biodegradable bio-composite films from soy protein and silk protein. Bio films are defined as flexible films prepared from biological materials such as protein. These materials have potential application in medical and food as a packaging material. Their use depends on various parameters such as mechanical (strength and modulus), thermal, among others. In this study, prepare and characterization of bio films made from Soy Protein Isolate (SPI) (matrix) and Silk Fiber (SF) (reinforcement) through solution casting method by the addition of plasticizer and crosslinking agent. The obtained SPI and SPI/SF composites were subsequently subjected to evaluate their mechanical and thermal properties by using Universal Testing Machine and Thermal Gravimetric Analyzer respectively. The tensile testing showed significant improvements in strength with increasing amount of SF content and the % elongation at break of the composites of the SPI/SF was lower than that of the matrix. Though the interfacial bonding was moderate, the improvement in tensile strength and modulus was attributed to the higher tensile properties of the silk fiber.

• Journal of Sericultural and Entomological Science
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• v.45 no.2
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• pp.121-125
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• 2003

Dissolution of Antheraea pernyi silk fiber was carried out in a zinc nitrate 6 hydrate (Zn(NO$_3$)$_2$ㆍ6$H_2O$) solution with various dissolving conditions. The solubility was significantly dependent on the concentration of zinc nitrate, dissolving temperature and time. Regenerated A. pernyi silk fibroin powder was obtained through dialysis process to remove chaotropic salt. FTIR and X-ray diffractometer showed that the conformation of regenerated A. pernyi silk powder was sheet structure.

• Journal of Sericultural and Entomological Science
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• v.40 no.1
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• pp.70-77
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• 1998

This study was carried out in order to find out the relationship between physical and chemical properties of silk fiber treated by concentrated calcium nitrate solution. The tensile, thermal and dynamic mechanical properties are also examined on Ca(NO3)2 treated silk fibers. The tensile properties of silk fibers treated by calcium nitrate changed with a concentration. The thermal behavior were also affected by the concentration of calcium nitrate. The degradation temperature (endotherms) and glass transition temperature shifted to lower temperature as the treated concentration increased. It is thought that the physical properties are strongly related to the structure and morphology of Ca(NO3)2 treated silk fibers. As a result, these give property changes with a concentration dependence.

• Proceedings of the Korean Fiber Society Conference
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• 1999.10a
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• pp.459-464
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• 1999

• Journal of Sericultural and Entomological Science
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• v.34 no.2
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• pp.32-40
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• 1992

Many studies have been carried out on the graft finishing in order to improve the quality of silk fiber. Various vinyl monomers, for instance, styrene, methylmethacrylate, 2-hydroxyeth-ylmethacrylate and methacrylamide, have been used practically up to date. Among these monomers, methacrylamide has been applied as the most favourable monomer onto silk fibers in recent years. The polymerization mechanism about styrene- and methylmethacrylate-grafted silk fiber has been studied by many researchers. They proposed that free radicals were formed and vinyl monomers were polymerized in silk fibroin by graft polymerization mechanism, while active sites were varied by the types of monomer and initiator as well as by the reaction condition. In general. there is another Opinion that monomers are polymerized and impregnated in the internal side of the fiber by homopolymerization, which has not been proved experimentally yet More than 10 years have been passed since methacrylamide was applied on the silk fiber, and at the present time most finishings are being achieved by methacrylamide. However, no attention has been paid to the polymerization mechanism of the methacrylamide-treated silk fiber yeL In this paper, the treatments of methacrylamide on silk fibers were studied in aqueous solution using potassium persulfate as an initiator. The polymerization mechanism of the methacrylamide-treated silk fibers was investigated and analyzed on the basis of the results of infrared spectroscopy, amino acid analysis and scanning electron microscopy. From the results of these instrumental analyses, it can be suggested that polymerization mechanism about the methacrylamide-treated silk fibers is not performed by graft polymerization which has been accepted generally in styrene and methylmethacrylate-grafted silk fibers. The different mechanism is supposed to be due to the difference in monomer types, initiator types and treatment conditions.

• Textile Coloration and Finishing
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• v.2 no.3
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• pp.36-42
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• 1990

The effect of urea and its derivatives, which are known as structure breakers of water, in the dyeing of silk with acid dyes was investigated. Without ureas the maximum dye uptake was observed at $60^{\circ}C$ when dyed with Milling Cyanine 5R, but in the presence of ureas the maximum dye uptake was decreased and its temperature also shifted to lower regin in the order of urea >N-methyl urea > N,N-dimethyl urea>tetramethyl urea. These tendencies were more marked in the dyeing of silk fiber with Orange II, a typical levelling type acid dye. These can be interpreted as the decrease in the hydropholic interaction between fiber molecules which results in the broadening of the inter micelie spacing. The increased partition coefficient of dye molecules in the bath by the action of ureas also contributes to dyeing results, and it can be seen from the spectral characteristics of dyes in aqueous urea solution.

• Journal of Sericultural and Entomological Science
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• v.39 no.1
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• pp.73-78
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• 1997

The crystalline fraction of silk fibroin (Fcp) was obtained from the aqueous solution of silk fibroin hydrolyzed by $\alpha$-chymotrypsin. The molecular weight of Fcp was found approximately 7000 by using high speed GPC. On the other hand, a high molecular weight of PIFcp product could be obtained by the reverse reaction of enzymatic proteolysis of Fcp precipitates. Some parts of this PIFcp have the molecular weights of approximately 17000 and 24000. As a result of x-ray diffraction analysis, the crystal structure of Fcp and PIFcp was turned out silk-II type and silk-I type, respectively. Upon the reverse reaction of enzymatic protelysis, the structural transition occured from silk-II type to silk-I type crystal for the most of Fcp precipitates. It was confirmed that PIFcp might be somewhat stable crystal structure of silk-I type according to the thermal analysis as well as x-ray diffraction method.