• Asian-Australasian Journal of Animal Sciences
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• 제6권1호
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• pp.45-51
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• 1993

With the purpose to utilize cage layer manure (CLM), whole crop corn forage was ensiled with 30% CLM (MS silage) and without CLM (CS silage). MS silage was significantly (p < 0.05) higher in pH value, total VFA, propionic and butyric acids, and the ratio of ammonia nitrogen to total nitrogen, but lower (p < 0.05) in lactic acid and water soluble carbohydrate (WSC) contents than CS silage. Digestibility was evaluated using Shiba strain Japanese goats. Urea was supplemented to CS silage at feeding to adjust nitrogen intake to MS silage (US silage). There were no differences in digestibilities of dry matter (DM) and energy among the three silages. However, US silage showed higher (p < 0.05) digestibility of crude protein, but digestibilities of NDF, ADF, hemicellulose and cellulose were higher (p < 0.05) in MS silage. Nitrogen retention was positive in US and MS silages, but it was negative in CS silage. Voluntary feed intake of goats was 11.02, 12.03 and 13.34 g of DM per metabolic body weight ($kg^{0.75}$) for 10 minutes, for CS, US and MS silages, respectively.

• 한국유기농업학회지
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• 제9권3호
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• pp.73-83
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• 2001

The qualities of the rice straw, black locust and orchardgrass silage added with grape pomace were investigated in this study and the amount of grape pomace added in different treatments were 0, 20, 40 and 50%, respectively. Crude protein contents of rice straw(6.5-10.6%) and orchardgrass silage(13.2-14.8%) added with grape pomace were significantly higher(p<0.05) than that of 100% rice straw(5.3%) and orchard grass silage(12.8%), however the crude protein contents(14.9-15.6%) of black locust silage in supplementation were lower than that of 100% black locust silage(16.3%). With higher amount of addition of grape pomade to rice straw, black locust and orchardgrass silages, ADF, NDF and crude ash contents decreased significantly(p<0.75). Moisture contents decreased according to the higher proportion of grape pomace in the rice straw silages, but increased according to the higher proportion of grape pomace in the black locust and orchardgrass silage. Values of pH in 20∼60% addition of grape pomace in rice straw(4.0∼4.2), black locust(4.1∼4.3) and orchardgrass silages(4.2∼4.4) were lower than that of 100% in rice straw(4.6), black locust(5.4) and orchardgrass silages(4.7). The contents of acetic acid and total volatile fatty acid significantly increased according to higher levels of addition of grape pomace compared to the respective values of silage. However the contents of lactic acid in supplementation of grape pomace were lower than that of 100% black locust silage.

• Animal Bioscience
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• 제34권1호
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• pp.48-55
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• 2021

Objective: This study was conducted to investigate the effects of wilting and microbial inoculant treatment on the fermentation pattern and quality of Italian ryegrass silage. Methods: Italian ryegrass was harvested at heading stage and ensiled into vinyl bags (20 cm×30 cm) for 60d. Italian ryegrass was ensiled with 4 treatments (NWNA, no-wilting noadditive; NWA, no-wilting with additive; WNA, wilting no-additive; WA, wilting with additive) in 3 replications, wilting time was 5 hours and additives were treated with 106 cfu/g of Lactobacillus plantarum. The silages samples were collected at 1, 2, 3, 5, 10, 20, 30, 45, and 60 days after ensiling and analyzed for the ensiling quality and characteristics of fermentation patterns. Results: Wilting treatment resulted in lower crude protein and in vitro dry matter digestibility and there were no significant differences in acid detergent fiber (ADF), total digestible nutrient (TDN), water-soluble carbohydrate (WSC), ammonia content, and pH (p>0.05). However, wilting treatment resulted in higher ADF and neutral detergent fiber content of Italian ryegrass silage (p<0.05), and the WNA treatment showed the lowest TDN and in vitro dry matter digestibility. The pH of the silage was higher in the wilting group (WNA and WA) and lower in the additive treatment group. Meanwhile, the decrease in pH occurred sharply between the 3-5th day of storage. The ammonia nitrogen content was significantly lower in the additive treatment (p<0.05), and wilting had no effect. As fermentation progressed, the lactic and acetic acid contents were increased and showed the highest content at 30 days of storage. Conclusion: The wilting treatment did not significantly improve the silage fermentation, but the inoculant treatment improved the fermentation patterns and quality of the silage. So, inoculation before ensiling is recommended when preparing high quality of Italian ryegrass silage, and when wilting, it is recommended to combine inoculation for making high quality silage.

• 한국초지조사료학회지
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• 제41권4호
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• pp.302-307
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• 2021

In the current study, lactic lactic acid bacteria (LAB) Lactobacillus plantarum and Pediococcus pentosaceus were used as a mixed additive for the production of Orchardgrass silage by ensiled method and nutritional change fermentation ability and microbial content of experimental silages. The addition of LAB to Orchardgrass during ensiling process rapidly reduced the pH of the silages than the non-inoculated silages. In addition, the lactic and acetic acid content of silage was increased by LAB strains than the non-inoculated silages whereas butyric acid content was reduced in silage treated with LAB. A microbiological study revealed that higher LAB but lower yeast counts were observed in inoculated silages compared to non-inoculated silage. Overall data suggested that the addition of LAB stains could have ability to induce the fermentation process and improve the silage quality via increasing lactic acid and decreasing undesirable microbes.

• Asian-Australasian Journal of Animal Sciences
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• 제18권6호
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• pp.807-811
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• 2005

This experiment examined the characteristics of fermented juice of epiphytic lactic acid bacteria (FJLB) prepared by the addition of glucose, sucrose and molasses as a fermentation substrate. The effect of FJLB on the fermentative quality and changes in chemical composition during fermentation of guineagrass silage were also investigated. The pH value of the silages treated with FJLB rapidly decreased, and reached to the lowest value within 7 days of start of fermentation, as compared to the control. The number of lactic acid bacteria (LAB) in the treated silages increased for the first 3 days, thereafter the number of LAB declined gradually up to the end of the experiment. Silages treated with FJLB had larger populations of LAB than the control. Ammonia-nitrogen production increased throughout the ensiling period, which in the control and no-sugar added FJLB silages were higher than the other treated silages. Lactic acid levels varied with the time of ensiling and among the silage treatments. For any sugar FJLB treated silages, the lactic acid increased initially, and then slightly reduced to less than 50 g/kg of dry matter until 49 days after ensiling, except the silage treated with glucose added FJLB. Nevertheless, lactic acid content of the control decreased constantly from the beginning of ensiling and was not found after 35 days. Moreover, acetic acid content increased throughout the ensiling period. All the FJLB treated silages had significantly (p<0.05) lower pH and ammonia-nitrogen content, while significantly (p<0.05) higher lactic acid content and V-score value compared with the control. This study confirmed that the applying of FJLB with any sugar substrate improved fermentative quality of silage.

• Asian-Australasian Journal of Animal Sciences
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• 제33권8호
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• pp.1273-1283
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• 2020

Objective: This study investigated a method of preparing corn stover for Irpex lacteus (I. lacteus) treatment to improve its in vitro rumen degradability under non-sterile conditions. Methods: Corn stover was inoculated with Lactobacillus plantarum (L. plantarum), Lactobacillus buchneri (L. buchneri), and an equal mixture of these strains, and ensiled for 0, 3, 7, 14, and 28 days. After each period, a portion of the silage was sampled to assess the silage quality, and another portion of the silage was further treated with I. lacteus at 28℃ for 28 d. All the samples were analyzed for fermentation quality, chemical composition, and in vitro gas production (IVGP) as a measure of rumen fermentation capacity. Results: Lactic acid bacteria (LAB) was found to improve the silage quality of the corn stover, and the corn stover silage inoculated with L. plantarum produced more lactic acid and higher IVGP than other silage groups. The I. lacteus colonies flourished in the early stage of corn stover silage, especially on the 3-d corn stover silage inoculated with both L. plantarum and L. buchneri. This led to an 18% decrease in the acid detergent lignin content, and a 49.6% increase in IVGP compared with the raw stover. Conclusion: The combination of ensiling with the mixed LAB inoculation and I. lacteus treatment provided a cost-effective method for the improvement of the IVGP of corn stover from 164.8 mL/g organic matter (OM) to 246.6 mL/g OM.

• 한국초지조사료학회지
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• 제37권3호
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• pp.189-194
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• 2017

The present study analyzes the role of Lactic Acid Bacteria Mixture (LBM) on improving rye silage quality. Rye of four different stages (Booting, Heading, Flowering, and Late flowering) was collected and silage was prepared. The nutrient profile analysis of experimental silage groups showed no significant changes between control and LBM inoculation. Interestingly, the pH of rye silage in LBM treatments showed significant reduction than control (p<0.05) in all stages of rye silage. However, lowest pH (3.69) resulted on booting stage among other stages of rye. Subsequently significant lactic acid production was noted in all stages of LBM inoculation than control. Conversely maximum lactic acid production of (5.33%DM) was noted at booting stage followed by (4.86%DM) in heading stage. Further the lactic acid bacterial (LAB) count in LBM inoculated group showed significant increase than control. Similarly, the silage of booting stage group registered maximum LAB population ($63.7{\times}10^6CFU/g$) after that heading stage ($32.3{\times}10^6CFU/g$). Further significant reduction in yeast growth and no fungal growth was noted in all LPM treatment groups. Hence, LBM inoculants could be a better additive for improving rye silage quality.

• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1273-1278
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• 2005

This study is to evaluate the effect of different levels of ensiling density on the fermentation quality of guineagrass silages during the early stage of ensiling. Guineagrass at the milky ripe stage was chopped and ensiled into a small-scale laboratory silo at two ensiling density levels (high density at 95 g/silo and low density at 75 g/silo). Three silos per level were opened after six ensiling periods (0.5, 1, 1.5, 2, 3 and 7 days of ensiling) and the fermentation qualities were analyzed. Within the initial 1.5 days of ensiling there were not significant (p>0.05) differences in the fermentation qualities between two density levels silages, and an almost constant pH and no or only small amounts of lactic acid, acetic acid and total volatile fatty acids were detected. However, the high density silage significantly (p<0.05) increased the rate and extent of fermentation after 1.5 days of ensiling, which was well reflected in significantly (p<0.05) faster and larger pH decline and lactic acid production at each elapsed time as compared with the low density silage. This resulted in significantly (p<0.05) lower finial pH and significantly (p<0.05) higher lactic acid content at the end of the experiment. Moreover, there was higher AA content relative to LA in both the H-D and L-D silages during the full fermentation course, and resulted in the AA-type silage. There were generally somewhat or significantly (p<0.05) higher acetic acid, volatile fatty acids and ammonia-N/total nitrogen in the high density silage than in the low density silage during the initial 3 days of ensiling. However, there were higher (p>0.05) ammonia-N/total nitrogen and significantly (p<0.05) higher butyric acid content in the low density silage at day 7 of ensiling. The silages of two density levels showed an initial increase in glucose between 0.5 and 1 day for the high density silage and between 1 and 1.5 days for the low density silage, respectively, thereafter showed a large decrease until the end of the experiment. There were not large differences (p>0.05) in ethanol content between the low density and high density silages that showed small amounts within initial 3 days of ensiling. However, the low density silage had a significantly (p<0.05) higher ethanol content than the high density silage at the end of experiment. From the above results it was suggested that the increase in ensiling density was an effective method to improve the fermentation quality, especially for tropical grasses.

• 한국초지조사료학회지
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• 제32권3호
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• pp.221-228
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• 2012

본 연구는 수수 ${\times}$ 수수 교잡종의 원형 곤포 및 트렌치 사일리지의 부위에 따른 사료가치 및 품질에 미치는 영향을 구명하기 위하여 천안에 위치한 국립축산과학원 자원개발부에서 수행되었다. 수수${\times}$ 수수 교잡종 SS405는 5월 초순에 파종한 다음 출수기에 수확하여 사일리지로 조제하였다. 원형 곤포사일리지의 조단백질, NDF 함량 및 in vitro 건물소화율은 트랜치 사일리지와 비슷한 수준을 보였으나 NDF 및 TDN 함량에서는 원형 곤포사일리지 보다 트랜치 사일리지에서 약간 증가하였다. 사일리지 부위별 조단백질 함량은 외부나 중간부위 보다 내부에서 약간의 감소를 보였으나(p<0.05), NDF, ADF 및 TDN 함량 및 in vitro 건물소화율에서는 차이를 보이지 않았다. 원형 곤포사일리지의 젖산 함량은 트랜치 사일리지 보다 유의적으로 감소하였으며(p<0.05) 초산 함량도 감소하는 경향을 보였을 뿐 유의차는 나타나지 않았다. 그리고 사일리지 부위별 젖산, 초산 및 낙산 함량은 큰 차이를 보이지 않았다.

• 한국초지조사료학회지
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• 제15권3호
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• pp.198-206
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• 1995

본 연구는 1991년부터 1993년까지 사료작물과 목초류 7종(호맥, 대맥, 연맥, Italian ryegrass, alfalfa, orchardgrass 및 혼과목초)을 공시재료로 하여 곤포사일리지(BS=baled silage making)조제이용을 위한 수확시기와 이때의 BS 생산성 및 품질을 평가하였다. 시험방법에 있어서 작물재배는 농촌진흥청의 사료작물 및 목초 표준경종방법에 준하여 실시하였다. 사일리지 조제작업중 BS는 자체적으로 제작한 각형 베일러를 이용 개체당 크기 90cm$\times$60cm$\times$50cm, 중량은 건물기준 20kg 내외, 곤포압착 21~23mm를 유지하여 조제하였다. 한편 고수분 재료는 수분정도에 따라 예건처리(무처리, 0.5일 및 1.0일)와 formic acid(무처리, 0.3% 및 0.6%)를 병행 처리하여 BS 를 조제하였다. 맨류작물의 BS 생산을 위한 수확적기는 대맥, 황숙기, 호맥과 연맥은 유숙기 이었으며 이때의 BS 수량은 각각 11.92톤(대맥), 12.64톤(호맥) 및 8.41톤/ha(연맥)이었다. Italian ryegrass 및 목초의 수확적기는 각 초종 모두 개화기로서 년간 BS 생산성은 Italian 13.81톤(2회 예취), 혼파목초 11.46톤(3회), alfalfa 10.62톤/ha(3회)이었다. 한편 조기수확에 따른 고수분 재료의 BS 품질은 무처리시의 35점에서 예건 및 FA 동시처리시에는 84점으로 향상되었다. Italian tyegrass의 BS품질도 무처리시의 61점에서 예건 및 FA 처리시에는 88점으로 개선되는 효과가 있었다.