• BMB Reports
• /
• 제53권3호
• /
• pp.154-159
• /
• 2020

We investigated the effects of physalin A, B, D, and F on osteoclastogenesis induced by receptor activator of nuclear factor κB ligand (RANKL). The biological functions of different physalins were first predicted using an in silico bioinformatic tool (BATMAN-TCM). Afterwards, we tested cell viability and cell apoptosis rate to analyze the cytotoxicity of different physalins. We analyzed the inhibitory effects of physalins on RANKL-induced osteoclastogenesis from mouse bone-marrow macrophages (BMMs) using a tartrate-resistant acid phosphatase (TRAP) stain. We found that physalin D has the best selectivity index (SI) among all analyzed physalins. We then confirmed the inhibitory effects of physalin D on osteoclast maturation and function by immunostaining of F-actin and a pit-formation assay. On the molecular level, physalin D attenuated RANKL-evoked intracellular calcium ([Ca(2+)](i)) oscillation by inhibiting phosphorylation of phospholipase Cγ2 (PLCγ2) and thus blocked the downstream activation of Ca2+/calmodulin-dependent protein kinases (CaMK)IV and cAMP-responsive element-binding protein (CREB). An animal study showed that physalin D treatment rescues bone microarchitecture, prevents bone loss, and restores bone strength in a model of rapid bone loss induced by soluble RANKL. Taken together, these results suggest that physalin D inhibits RANKL-induced osteoclastogenesis and bone loss via suppressing the PLCγ2-CaMK-CREB pathway.

• Journal of Ginseng Research
• /
• 제48권3호
• /
• pp.323-332
• /
• 2024

Background: Studies have reported that the combination of two or more therapeutic compounds at certain ratios has more noticeable pharmaceutical properties than single compounds and requires reduced dosage of each agent. Red ginseng and velvet antler have been extensively used in boosting immunity and physical strength and preventing diseases. Thus, this study was conducted to elucidate the skin-protective potentials of red ginseng extract (RGE) and velvet antler extract (VAE) alone or in combination on ultraviolet (UVB)-irradiated human keratinocytes and SKH-1 hairless mice. Methods: HaCaT cells were preincubated with RGE/VAE alone or in combination for 2 h before UVB (30 mJ/cm²) irradiation. SKH-1 mice were orally given RGE/VAE alone or in combination for 15 days before exposure to single dose of UVB (600 mJ/cm²). Treated cells and treated skin tissues were collected and subjected to subsequent experiments. Results: RGE/VAE pretreatment alone or in combination significantly prevented UVB-induced cell death, apoptosis, reactive oxygen species production, and DNA damage in keratinocytes and SKH-1 mouse skins by downregulating mitogen-activated protein kinases/activator protein 1/nuclear factor kappa B and caspase signaling pathways. These extracts also strengthened the antioxidant defense systems and skin barriers in UVB-irradiated HaCaT cells and SKH-1 mouse skins. Furthermore, RGE/VAE co-administration appeared to be more effective in preventing UVB-caused skin injury than these extracts used alone. Conclusion: Overall, these findings suggest that the consumption of RGE/VAE, especially in combination, offers a protective ability against UVB-caused skin injury by preventing inflammation and apoptosis and enhancing antioxidant capacity.

• 컴퓨터교육학회논문지
• /
• 제12권4호
• /
• pp.47-55
• /
• 2009

모바일 그리드에서 모바일 장치를 작업 처리에 이용하고자 하는 연구들이 많이 이루어지고는 있지만 모바일 장치는 무선 연결 및 배터리 용량에 관한 제약을 가지고 있으므로 모바일 장치를 이용한 작업 처리는 기존 그리드 환경에서의 작업 처리에 비해 신뢰성 및 효율성이 낮다. 따라서 모바일 장치가 가지는 제약 사항들을 고려한 작업 처리 방법이 필요하다. 이 논문에서는 모바일 그리드 환경에서 작업 이주 기법을 통해 모바일 장치를 이용하여 작업을 수행하는 방법을 제안한다. 즉, 모바일 장치에서 작업 수행 시 문제가 되는 상황들을 미리 예측하고 실행중인 작업을 체크포인팅하여, 모바일 장치에 문제가 발생했을 경우 체크포인팅 정보를 이용하여 다른 모바일 장치에게 작업을 이주할 수 있도록 한다. 이를 위해 프록시 서버에는 모바일 장치 관리자를 두고 모바일 장치에는 상태 관리자를 두며, 두 관리자를 통해 모바일 장치의 접속, 무선 신호 세기, 배터리 용량을 확인한다. 시뮬레이션 결과는 제안한 작업 이주 방법이 작업 수행시 효율성 및 신뢰성을 높일 수 있음을 보여준다.

• 한국전자통신학회논문지
• /
• 제14권6호
• /
• pp.1061-1068
• /
• 2019

협력 네트워크는 송신기로부터 수신기로 신호를 전송할 때 여러 중계기를 이용하여 신호를 전송하고 수신기에서 이 신호를 결합하여 복조함으로써 통신성능 향상과 데이터 전송률을 높이는 장점을 제공한다. 일반적으로 송신기에서 수신기로 신호를 전송할 때 중계기가 송신기로부터 수신된 신호를 증폭/재변조 후 수신기로 보내는 반복적인 신호 전송형태가 널리 사용된다. 본 논문에서는 두 가지 신호전송 방식을 혼합하여 신호를 전송하는 협력 네트워크에서의 성능을 분석한다. 즉, 송신기와 중계기사이에서는 일반 변조방식을 사용하고, 중계기와 수신기 사이에서는 공간 시간 코드 방식을 적용한다. 변조방식은 시스템의 복잡도를 줄이기 위해 채널상태정보를 이용하지 않는 차등변조 방식을 이용한다. 다양한 개수의 중계기가 있을 경우에 대한 성능을 비트오류율을 이용하여 분석하며, 송신기와 중계기 및 중계기와 수신기 사이에서 서로 다른 세기로 신호를 보낼 때의 성능을 비교한다. 또한 다양한 신호세기 및 중계기의 개수를 동시에 고려한 성능에 대해서도 논의한다.

• 대한한의학방제학회지
• /
• 제30권4호
• /
• pp.269-280
• /
• 2022

Objectives : In this study, it was investigated the effects of solid-phase fermentation extraction with Phellinus linteus of discarded Schisandra chinensis extract (PS) and its action mechanism on dexamethasone-induced muscle atrophy in mice. Methods : In mice, muscle atrophy model was induced by dexamethasone (5 mg/kg, I.p) once daily for 2 weeks and with PS extract administration (100 and 300 mg/kg, p.o.) as treatment groups. The changes in body weights, grip strength, Treadmill test, muscle weights, and the expression of atrophy-related genes were measured in muscle atrophy mice. The histological changes of gastrocnemius tissues were also observed by H&E staining with measurement of myofiber size. Results : The administration of PS extract increased significantly body weights, grip strength, treadmill test and muscle weights in muscle atrophy mice. PS extract administration increased significantly the area of myofibers and inhibited structural damages of muscle and increased significantly the expression of myogenin and decreased significantly the expression of MuRF1, Atrogin1 and phosphorylation of AMPK and PGC1α in muscle tissues of muscle atrophy mice. Conclusions : These results indicate that PS extract has a improvement effects on muscle atrophy with stimulation of myogenic differentiation and inhibition of mRNA degradation that could be related with the activation of AMPK and PGC1α signaling pathways in muscle. This suggests that PS extract can apply to treat muscle atrophy in clinics.

• Fisheries and Aquatic Sciences
• /
• 제26권6호
• /
• pp.406-422
• /
• 2023

Sarcopenia is an age-related, progressive skeletal muscle disorder involving the loss of muscle mass and strength. Previous studies have shown that γ-aminobutyric acid (GABA) from fermented oysters aids in regulatory T cells (Tregs) cell expansion and function by enhancing autophagy, and concomitantly mediate muscle regeneration by modulating muscle inflammation and satellite cell function. The fermentation process of oysters not only increases the GABA content but also enhances the content of branched amino acids and free amino acids that aid the level of protein absorption and muscle strength, mass, and repair. In this study, the effect of GABA-enriched fermented sarco oyster extract (FSO) on reduced muscle mass and functions via Treg modulation and enhanced autophagy in aged mice was investigated. Results showed that FSO enhanced the expression of autophagy markers (autophagy-related gene 5 [ATG5] and GABA receptor-associated protein [GABARAP]), forkhead box protein 3 (FoxP3) expression, and levels of anti-inflammatory cytokines (interleukin [IL]-10 and transforming growth factor [TGF]-β) secreted by Tregs while reducing pro-inflammatory cytokine levels (IL-17A and interferon [IFN]-γ). Furthermore, FSO increased the expression of IL-33 and its receptor IL-1 receptor-like 1 (ST2); well-known signaling pathways that increase amphiregulin (Areg) secretion and expression of myogenesis markers (myogenic factor 5, myoblast determination protein 1, and myogenin). Muscle mass and function were also enhanced via FSO. Overall, the current study suggests that FSO increased autophagy, which enhanced Treg accumulation and function, decreased muscle inflammation, and increased satellite cell function for muscle regeneration and therefore could decrease the loss of muscle mass and function with aging.

• 인터넷정보학회논문지
• /
• 제23권3호
• /
• pp.13-20
• /
• 2022

차세대 와이파이 표준기술인 IEEE 802.11ay는 밀리미터파 대역에서 AP (Access Point)가 다수의 STA (Station)로 동시에 데이터를 전송하도록 MU-MIMO (Multiple User Multiple Input Multiple Output) 통신을 지원한다. 이를 위해, 주기적으로 MU-MIMO 빔포밍 훈련을 수행해야 하고, 효율적인 빔포밍 훈련을 위해서는 AP가 다수의 안테나로 다수의 빔을 동시에 전송할 때, 각 STA에서 측정되는 신호 세기를 정확히 예측하는 것이 중요하다. 본 논문에서는 딥러닝 기반 다중 빔 전송링크 성능 예측기법을 제안한다. 제안한 예측기법은 특정 실내 또는 실외 환경에서 미리 학습된 딥러닝 모델을 이용하여 다수의 빔이 동시에 전송될 때 STA에서 측정되는 신호 세기 예측의 정확성을 높인다. 이때, 딥러닝의 입력으로 개별 빔이 전송될 때 STA에서 측정되는 신호 세기 정보를 이용하고, 개별 빔의 신호 세기 정보를 얻는 과정은 이미 기존의 빔포밍 훈련에 포함되어 있으므로 정보 수집을 위해 추가적인 비용을 발생하지 않는다. 성능평가를 위해 NIST (National Institute of Standards and Technology)에 의해 개발된 Q-D 채널구현 (Quasi-Deterministic Channel Realization) 오픈소스 소프트웨어를 활용하였고 실측 데이터 기반으로 밀리미터파 채널을 구현하였다. 실험결과에서는 제안한 예측기법이 다른 비교기법보다 향상된 예측성능을 보였다.

• 한국정보통신학회논문지
• /
• 제7권1호
• /
• pp.75-82
• /
• 2003

인터넷을 이용한 여러 응용 서비스들 중에서 저렴한 비용으로 음성을 전송할 수 있는 VoIP 서비스의 발전으로 사용자의 급격한 증가가 예상된다. VoIP에 mobility, universal number, multiparty conference, voice mail, automatic call distribution과 같은 고품질의 서비스를 제공하기 위해서는 시그널링이 가능한 표준화된 프로토콜이 필요하다. 현재 IETF의 SIP(Session Initiation Protocol)가 빠른 호 설정과 parsing 및 compile이 쉬운 장점으로 인해 SIP를 기반으로 한 VoIP 서비스를 제공하기 위해 국내외적으로 SIP 기반 구성요소에 대한 개발이 진행 중이다. 본 논문에서는 사용자가 보내는 Request(INVITE) 메소(method)를 처리해주는 SIP 서버의 부하 경감, 망 운용의 효율성, 많은 사용자에 대한 서비스를 제공하기 위해 새로운 서버 유형인 Hybrid형 SIP 서버를 제안하고, 이를 이용하여 새로운 방식의 인터넷폰을 설계 하였다.

• The Plant Pathology Journal
• /
• 제19권1호
• /
• pp.30-33
• /
• 2003

The chestnut blight fungus, Cryphonectria parasitica, and its hypovirus aye a useful model system in the study of the mechanisms of hypoviral infection and its consequences, such as a biological control of fungal pathogens. Strains containing the double-stranded (ds) RNA viruses Cryphonectria hypovirus 1 show characteristic symptoms of hypovirulence and display hypovirulence-associated changes, such as reduced pigmentation, sporulation, laccase production, and oxalate accumulation. Interestingly, symptoms caused by hypoviral infection appear to be the result of aberrant expression of a number of specific genes in the hypovirulent strain. Several viral regulated fungal genes are identified as cutinase gene, Lac1, which encodes an extracellular laccase, Crp, which encodes an abundant tissue-specific cell-surface hydrophobin that mediates physical strength, and Mf2/1 and Mf2/2, which encode pheromone genes involved in poor sporulation in the presence of hypo-virus. Since the phenotypic changes in the fungal host are pleiotropic, although coordinated and specific, it has been suggested that the hypovirus disturbs one or several regulatory pathways (Nuss,1996). Accordingly, several studies have shown the implementation of a signal transduction pathway during viral symptom development. Although further studies are required, hypovirulence and its associated symptom development due to the hypoviral regulation of a fungal hetero-trimeric G-protein have been suggested. In addition, recent studies have shown the presence of a novel protein kinase gene cppk1 and its transcriptional upregulation by hypovirus. In this review, the presence of important components in signal transduction pathway, their putative biological function, and viral-specific regulation will be addressed.

• Journal of Information Processing Systems
• /
• 제14권3호
• /
• pp.771-789
• /
• 2018

In heterogeneous wireless networks supporting multi-access services, selecting the best network from among the possible heterogeneous connections and providing seamless service during handover for a higher Quality of Services (QoSs) is a big challenge. Thus, we need an intelligent vertical handover (VHO) decision using suitable network parameters. In the conventional VHOs, various network parameters (i.e., signal strength, bandwidth, dropping probability, monetary cost of service, and power consumption) have been used to measure network status and select the preferred network. Because of various parameter features defined in each wireless/mobile network, the parameter conversion between different networks is required for a handover decision. Therefore, the handover process is highly complex and the selection of parameters is always an issue. In this paper, we present how to maximize network utilization as more than one target network exists during VHO. Also, we show how network parameters can be imbedded into IEEE 802.21-based signaling procedures to provide seamless connectivity during a handover. The network simulation showed that QoS-effective target network selection could be achieved by choosing the suitable parameters from Layers 1 and 2 in each candidate network.