• BMB Reports
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• v.53 no.3
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• pp.154-159
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• 2020

We investigated the effects of physalin A, B, D, and F on osteoclastogenesis induced by receptor activator of nuclear factor κB ligand (RANKL). The biological functions of different physalins were first predicted using an in silico bioinformatic tool (BATMAN-TCM). Afterwards, we tested cell viability and cell apoptosis rate to analyze the cytotoxicity of different physalins. We analyzed the inhibitory effects of physalins on RANKL-induced osteoclastogenesis from mouse bone-marrow macrophages (BMMs) using a tartrate-resistant acid phosphatase (TRAP) stain. We found that physalin D has the best selectivity index (SI) among all analyzed physalins. We then confirmed the inhibitory effects of physalin D on osteoclast maturation and function by immunostaining of F-actin and a pit-formation assay. On the molecular level, physalin D attenuated RANKL-evoked intracellular calcium ([Ca(2+)](i)) oscillation by inhibiting phosphorylation of phospholipase Cγ2 (PLCγ2) and thus blocked the downstream activation of Ca2+/calmodulin-dependent protein kinases (CaMK)IV and cAMP-responsive element-binding protein (CREB). An animal study showed that physalin D treatment rescues bone microarchitecture, prevents bone loss, and restores bone strength in a model of rapid bone loss induced by soluble RANKL. Taken together, these results suggest that physalin D inhibits RANKL-induced osteoclastogenesis and bone loss via suppressing the PLCγ2-CaMK-CREB pathway.

• Journal of Ginseng Research
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• v.48 no.3
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• pp.323-332
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• 2024

Background: Studies have reported that the combination of two or more therapeutic compounds at certain ratios has more noticeable pharmaceutical properties than single compounds and requires reduced dosage of each agent. Red ginseng and velvet antler have been extensively used in boosting immunity and physical strength and preventing diseases. Thus, this study was conducted to elucidate the skin-protective potentials of red ginseng extract (RGE) and velvet antler extract (VAE) alone or in combination on ultraviolet (UVB)-irradiated human keratinocytes and SKH-1 hairless mice. Methods: HaCaT cells were preincubated with RGE/VAE alone or in combination for 2 h before UVB (30 mJ/cm²) irradiation. SKH-1 mice were orally given RGE/VAE alone or in combination for 15 days before exposure to single dose of UVB (600 mJ/cm²). Treated cells and treated skin tissues were collected and subjected to subsequent experiments. Results: RGE/VAE pretreatment alone or in combination significantly prevented UVB-induced cell death, apoptosis, reactive oxygen species production, and DNA damage in keratinocytes and SKH-1 mouse skins by downregulating mitogen-activated protein kinases/activator protein 1/nuclear factor kappa B and caspase signaling pathways. These extracts also strengthened the antioxidant defense systems and skin barriers in UVB-irradiated HaCaT cells and SKH-1 mouse skins. Furthermore, RGE/VAE co-administration appeared to be more effective in preventing UVB-caused skin injury than these extracts used alone. Conclusion: Overall, these findings suggest that the consumption of RGE/VAE, especially in combination, offers a protective ability against UVB-caused skin injury by preventing inflammation and apoptosis and enhancing antioxidant capacity.

• The Journal of Korean Association of Computer Education
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• v.12 no.4
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• pp.47-55
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• 2009

There are many researches considering mobile devices as resources in mobile grids. However, the mobile device has some limitations: wireless connection and battery capacity. So, the grid operations using mobile devices have lower reliability and efficiency than those in fixed grid environments. In this paper, we propose a job migration scheme using mobile devices to overcome these limitations. The proposed job migration scheme predicts failure condition during execution and takes checkpoints. Then, if the failure occurs on mobile device during execution, the executing job can be migrated to other mobile device by checkpoint information. To perform the proposed migration scheme, we establish a mobile device manager on a proxy server and a status manager on a mobile device. Connection, wireless signal strength and battery capacity of mobile devices are identified through two managers. The simulation results show improvement of efficiency and reliability during execution.

• The Journal of the Korea institute of electronic communication sciences
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• v.14 no.6
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• pp.1061-1068
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• 2019

Cooperative networks transmit signals form the source node to the destination node via several relay node where the combining and demodulation of relay aided signals provide the benefit of performance enhancement and data rate increment. In general, a repetitive manner transmission scheme in which the received signal from the source node is amplified/re-generated and forward to the destination node is widely used. In this paper, we analyzed the performance of cooperative networks using the mixed transmission scheme. The conventional modulation scheme is used in the source-relay links, and space-time code is applied in the relay-destination links. To reduce the complexity of the overall system, we adopt differential modulation which bypasses channel state information. We analyze bit error rate (BER) of the proposed system by considering the number of relay nodes, and the performances depending on the strength of transmission signal in the source-relays and rely-destination links are compared. In addition, we also discuss the system performance with the signal strength and the number of relay nodes simultaneously.

• Herbal Formula Science
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• v.30 no.4
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• pp.269-280
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• 2022

Objectives : In this study, it was investigated the effects of solid-phase fermentation extraction with Phellinus linteus of discarded Schisandra chinensis extract (PS) and its action mechanism on dexamethasone-induced muscle atrophy in mice. Methods : In mice, muscle atrophy model was induced by dexamethasone (5 mg/kg, I.p) once daily for 2 weeks and with PS extract administration (100 and 300 mg/kg, p.o.) as treatment groups. The changes in body weights, grip strength, Treadmill test, muscle weights, and the expression of atrophy-related genes were measured in muscle atrophy mice. The histological changes of gastrocnemius tissues were also observed by H&E staining with measurement of myofiber size. Results : The administration of PS extract increased significantly body weights, grip strength, treadmill test and muscle weights in muscle atrophy mice. PS extract administration increased significantly the area of myofibers and inhibited structural damages of muscle and increased significantly the expression of myogenin and decreased significantly the expression of MuRF1, Atrogin1 and phosphorylation of AMPK and PGC1α in muscle tissues of muscle atrophy mice. Conclusions : These results indicate that PS extract has a improvement effects on muscle atrophy with stimulation of myogenic differentiation and inhibition of mRNA degradation that could be related with the activation of AMPK and PGC1α signaling pathways in muscle. This suggests that PS extract can apply to treat muscle atrophy in clinics.

• Fisheries and Aquatic Sciences
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• v.26 no.6
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• pp.406-422
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• 2023

Sarcopenia is an age-related, progressive skeletal muscle disorder involving the loss of muscle mass and strength. Previous studies have shown that γ-aminobutyric acid (GABA) from fermented oysters aids in regulatory T cells (Tregs) cell expansion and function by enhancing autophagy, and concomitantly mediate muscle regeneration by modulating muscle inflammation and satellite cell function. The fermentation process of oysters not only increases the GABA content but also enhances the content of branched amino acids and free amino acids that aid the level of protein absorption and muscle strength, mass, and repair. In this study, the effect of GABA-enriched fermented sarco oyster extract (FSO) on reduced muscle mass and functions via Treg modulation and enhanced autophagy in aged mice was investigated. Results showed that FSO enhanced the expression of autophagy markers (autophagy-related gene 5 [ATG5] and GABA receptor-associated protein [GABARAP]), forkhead box protein 3 (FoxP3) expression, and levels of anti-inflammatory cytokines (interleukin [IL]-10 and transforming growth factor [TGF]-β) secreted by Tregs while reducing pro-inflammatory cytokine levels (IL-17A and interferon [IFN]-γ). Furthermore, FSO increased the expression of IL-33 and its receptor IL-1 receptor-like 1 (ST2); well-known signaling pathways that increase amphiregulin (Areg) secretion and expression of myogenesis markers (myogenic factor 5, myoblast determination protein 1, and myogenin). Muscle mass and function were also enhanced via FSO. Overall, the current study suggests that FSO increased autophagy, which enhanced Treg accumulation and function, decreased muscle inflammation, and increased satellite cell function for muscle regeneration and therefore could decrease the loss of muscle mass and function with aging.

• Journal of Internet Computing and Services
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• v.23 no.3
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• pp.13-20
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• 2022

IEEE 802.11ay Wi-Fi is the next generation wireless technology and operates in mmWave band. It supports the MU-MIMO (Multiple User Multiple Input Multiple Output) transmission in which an AP (Access Point) can transmit multiple data streams simultaneously to multiple STAs (Stations). To this end, the AP should perform MU-MIMO beamforming training with the STAs. For efficient MU-MIMO beamforming training, it is important for the AP to estimate signal strength measured at each STA at which multiple beams are used simultaneously. Therefore, in the paper, we propose a deep learning-based link quality estimation scheme. Our proposed scheme estimates the signal strength with high accuracy by utilizing a deep learning model pre-trained for a certain indoor or outdoor propagation scenario. Specifically, to estimate the signal strength of the multiple concurrent beams, our scheme uses the signal strengths of the respective single beams, which can be obtained without additional signaling overhead, as the input of the deep learning model. For performance evaluation, we utilized a Q-D (Quasi-Deterministic) Channel Realization open source software and extensive channel measurement campaigns were conducted with NIST (National Institute of Standards and Technology) to implement the millimeter wave (mmWave) channel. Our simulation results demonstrate that our proposed scheme outperforms comparison schemes in terms of the accuracy of the signal strength estimation.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.7 no.1
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• pp.75-82
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• 2003

We can foresee the rapidly growing of users by development of VoIP service what can transmit a audio traffic with low cost among a lots application using internet. VoIP needs a standardized protocol that is able to do signaling for offering high quality of services such as mobility, universal number, multiparty conference, voice mail, automatic call distribution. At the present time, a base composition elements of SIP(Session Intiation Protocol) are developing for offering VoIP based SIP in the inside and outside of the country, because SIP of IETF which has a strength from 'fast connection', 'parsing' & 'easy to compile' points of view. This paper suggests a type of Hybrid SIP Server for providing some services as 'a reducing load of SIP server that process a request method from users', 'efficiency of managing networks', 'offering services to many users'.

• The Plant Pathology Journal
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• v.19 no.1
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• pp.30-33
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• 2003

The chestnut blight fungus, Cryphonectria parasitica, and its hypovirus aye a useful model system in the study of the mechanisms of hypoviral infection and its consequences, such as a biological control of fungal pathogens. Strains containing the double-stranded (ds) RNA viruses Cryphonectria hypovirus 1 show characteristic symptoms of hypovirulence and display hypovirulence-associated changes, such as reduced pigmentation, sporulation, laccase production, and oxalate accumulation. Interestingly, symptoms caused by hypoviral infection appear to be the result of aberrant expression of a number of specific genes in the hypovirulent strain. Several viral regulated fungal genes are identified as cutinase gene, Lac1, which encodes an extracellular laccase, Crp, which encodes an abundant tissue-specific cell-surface hydrophobin that mediates physical strength, and Mf2/1 and Mf2/2, which encode pheromone genes involved in poor sporulation in the presence of hypo-virus. Since the phenotypic changes in the fungal host are pleiotropic, although coordinated and specific, it has been suggested that the hypovirus disturbs one or several regulatory pathways (Nuss,1996). Accordingly, several studies have shown the implementation of a signal transduction pathway during viral symptom development. Although further studies are required, hypovirulence and its associated symptom development due to the hypoviral regulation of a fungal hetero-trimeric G-protein have been suggested. In addition, recent studies have shown the presence of a novel protein kinase gene cppk1 and its transcriptional upregulation by hypovirus. In this review, the presence of important components in signal transduction pathway, their putative biological function, and viral-specific regulation will be addressed.

• Journal of Information Processing Systems
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• v.14 no.3
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• pp.771-789
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• 2018

In heterogeneous wireless networks supporting multi-access services, selecting the best network from among the possible heterogeneous connections and providing seamless service during handover for a higher Quality of Services (QoSs) is a big challenge. Thus, we need an intelligent vertical handover (VHO) decision using suitable network parameters. In the conventional VHOs, various network parameters (i.e., signal strength, bandwidth, dropping probability, monetary cost of service, and power consumption) have been used to measure network status and select the preferred network. Because of various parameter features defined in each wireless/mobile network, the parameter conversion between different networks is required for a handover decision. Therefore, the handover process is highly complex and the selection of parameters is always an issue. In this paper, we present how to maximize network utilization as more than one target network exists during VHO. Also, we show how network parameters can be imbedded into IEEE 802.21-based signaling procedures to provide seamless connectivity during a handover. The network simulation showed that QoS-effective target network selection could be achieved by choosing the suitable parameters from Layers 1 and 2 in each candidate network.