• Title/Summary/Keyword: Signal site

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A Method for Identifying Splice Sites and Translation Start Sites in Human Genomic Sequences

  • Kim, Ki-Bong;Park, Kie-Jung;Kong, Eun-Bae
    • BMB Reports
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    • v.35 no.5
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    • pp.513-517
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    • 2002
  • We describe a new method for identifying the sequences that signal the start of translation, and the boundaries between exons and introns (donor and acceptor sites) in human mRNA. According to the mandatory keyword, ORGANISM, and feature key, CDS, a large set of standard data for each signal site was extracted from the ASCII flat file, gbpri.seq, in the GenBank release 108.0. This was used to generate the scoring matrices, which summarize the sequence information for each signal site. The scoring matrices take into account the independent nucleotide frequencies between adjacent bases in each position within the signal site regions, and the relative weight on each nucleotide in proportion to their probabilities in the known signal sites. Using a scoring scheme that is based on the nucleotide scoring matrices, the method has great sensitivity and specificity when used to locate signals in uncharacterized human genomic DNA. These matrices are especially effective at distinguishing true and false sites.

A Study on the Facility Layout and Signal-fire Stand of Border Signal-fire in Chosun Dynasty (조선시대 연변봉수(沿邊烽燧)의 배치형식 및 연대(煙臺)에 관한 연구)

  • Lee, Chul-Young;Yoon, Jae-Woong
    • Journal of architectural history
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    • v.15 no.5
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    • pp.41-56
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    • 2006
  • Signal fire was a communication system which deliver urgent news by smoke and light. It informed the condition of the border to the central government and the military base rapidly. This research was to analyze the border signal-fire which is the main facility of military protective duty for safety of sea side in chosun dynasty. The results of this research were described separately as follows. 1. The border signal-fire has three types of facility layout, The majority was one-site, near two-site, separate type. The majority was near two-site type. 2. Plan configuration of signal-fire stand which is separated with circle, oval, round-rectangular and rectangular type roughly was constructed with circle type generally. Four types of signal-fire stand was observed in southern and western sea side impartially. However, in eastern side, circle type of signal-fire stand was mainly found. Therefore, regional characteristics could be considered partially. 3. The height of signal-fire stand was about 8~10m. The diameter of combustion chamber on the signal-fire stand was 2~3m in circle type, and the dimension of rectangular type was about $1.9{\times}1.9{\sim}3.2{\times}3.0m$. 4. Building base that protect ground moisture and infilteration of rainfall was found in 10 border singal-fire, the height of it was about 0.3m~2.5m.

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Signal Sequence Prediction Based on Hydrophobicity and Substitution Matrix (소수성과 치환행렬에 기반한 신호서열 예측)

  • Chi, Sang-Mun
    • Journal of KIISE:Software and Applications
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    • v.34 no.7
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    • pp.595-602
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    • 2007
  • This paper proposes a method that discriminates signal peptide and predicts the cleavage site of the secretory proteins cleaved by the signal peptidase I. The preprocessing stage uses hydrophobicity scales of amino acids in order to predict the presence of signal sequence and the cleavage site. The preprocessing enhances the performance of the prediction method by eliminating the non-secretory proteins in the early stage of prediction. for the effective use of support vector machine for the signal sequence prediction, the biologically relevant distance between the amino acid sequences is defined by using the hydrophobicity and substitution matrix; the hydrophobicity can be used to Predict the location of amino acid in a cell and the substitution matrix represents the evolutionary relationships of amino acids. The proposed method showed 98.9% discrimination rates from signal sequences and 88% correct rate of the cleavage site prediction on Swiss-Prot release 50 protein database using the 5-fold-cross-validation. In the comparison tests, the proposed method has performed significantly better than other prediction methods.

Analysis on the nucleotide sequence of the signal region of bacillus subitilis extracellular cellulase gene (Bacillus subtilis로 부터 분리한 cellulase 유전자의 조절부위에 대한 염기서열분석)

  • 서연수;이영호;백운화;강현삼
    • Korean Journal of Microbiology
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    • v.24 no.3
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    • pp.236-242
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    • 1986
  • The nucleotide sequence of the genetic control site of Bacillus subtilis gene for $(1-4)-{\beta}-D-glucan$ endoglucanase (cellulase) was determined according to the procedures of the dideoxy chain termination method(Sanger et. al., 1977). The deduced amino acid sequence of this enzyme has a hydrophobic signal peptide at the $NH_2$ terminus similar to those found in fifteen other extracellualr enzymes from Bacillus species. This is followed by a sequence resembling the Bacillus ribosome binding site 14 nucleotide before the first codon of the gene. The presumptive promoter sequence was located 92 base pairs upstream fromthe initiation codon. The homology region in signal sequences was striking when comparing all the signal sequences of sixteen extracellular enzymes from Bacillus species so far compiled.

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Removal of Clutter from Doppler Radar Signal to Measure Accurate Muzzle Velocity (도플러 레이더를 이용한 포구속도 계측 시 클러터 제거 방법)

  • Kim, Hyoung-rae
    • Journal of Advanced Navigation Technology
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    • v.23 no.2
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    • pp.142-150
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    • 2019
  • Muzzle Velocity is one of the most important measurement items for evaluation of ammunition. The muzzle velocity is defined as the velocity when the projectile leaves the muzzle. Particularly, since the muzzle velocity is closely related to the performance of the propellant, precise measurement of muzzle velocity is required. Doppler radar is used to measure the muzzle velocity, but the quality of Doppler radar signal depends on the test site environment. In this paper, a method to remove the clutter that degrades the signal quality of Doppler radar by improving the structure of the test site and the signal processing method is suggested. For the application of the improved signal processing method, a program for acquiring Doppler radar's raw Doppler data was created. Statistical verification of the velocity data obtained through the improvement of the test site structure and signal processing method proved that the proposed method is effective for the removal of clutter as compared with the existing method.

Physical Mapping of rDNAs Using McFISH in Anemarrhena asphodeloides Bunge (지모에서 McFISH를 이용한 rDNAs의 물리지도 작성)

  • Kim, Soo-Young;Choi, Hae-Woon;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.6
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    • pp.515-518
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    • 2004
  • Anemarrhena asphodeloides, a medicinal plant, has chromosome number of 2n=2x=22. To characterize the somatic metaphase chromosomes, physical mapping of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) was applied. Two pairs of 45S rDNA loci were detected on the terminal regions of the short arm of chromosomes 1 and 3. A pair of 5S rDNA signal was observed on the short arm of chromosome 3. 5S rDNA site seemed to be the same locus as one of the 45S rDNA site. McFISH was very useful tool for the localization and identification of rDNAs on the metaphase chromosomes in A. asphodeloides.

3' end of putative sequences of the packaging signal in moloney-murine leukemia virus (Moloney murine Leukemia Virus에서 포장신호의 가능한 3' 끝의 염기서열)

  • 박종상
    • Korean Journal of Microbiology
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    • v.26 no.2
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    • pp.101-105
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    • 1988
  • 6M-MuLV mutants containing deldtions around the putative packaging signal were constructed by using recombinant DNA technique and transfected into NIH/3T3 cell. 2 of 6 mutants can not be packaged into virions even in the presence of the wild type helper virus. The boundary between the packagible and the non-packagible genome is located around Pvu I site, 421 nucleotide downstream from the 5' end of M-MuLV genome. 10 base pair inverted repeat sequence (GAGUCCAAAA) which can make stem structure around Pvu Isite could be the putative packaging signal.

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Nonlinearity Detection and Compensation in Radio over Fiber Systems Using a Monitoring Channel

  • Kim, Sung-Man
    • Journal of information and communication convergence engineering
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    • v.13 no.3
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    • pp.167-171
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    • 2015
  • A radio over fiber (RoF) system is a kind of analog optical transmission system and considered as a strong candidate for the next-generation fronthaul link in the future mobile network. In RoF systems, nonlinearity compensation is essential to increase the link capacity. In this paper, we propose a nonlinearity detection and compensation scheme using a monitoring channel in RoF systems. A monitoring channel is added at the transmitter site and used for transmitting a reference signal in an RoF transmission. The nonlinearity in the RoF transmission is detected by comparing the received monitoring signal and the original reference signal at the receiver site. Finally, the nonlinearity is compensated at the receiver by giving the reverse function of the detected nonlinearity. Our results show that the proposed scheme can almost remove the error vector magnitude degradation induced by the nonlinearity in the RoF system.

Functional Importance of TRAF6-Binding Motif in IL -1 Mediated Signal Transduction

  • Yim , Mi-Jung
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.311.1-311.1
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    • 2002
  • Crystal structure of TRAF6 in complex with TRAF6-binding sites from CD40 was previously determined. The structure revealed a distinct TRAF6-binding groove of CD40. the key structural determinant of interaction. The structural information leads to a proposed TRAF6-binding motif. This allows the identification of TRAF6-binding sequences in the hlRAK protein, whose functional requirement in IL-1 mediated signal transduction is further demonstrated using site-directed mutagenesis. The mutational in IL-1 mediated signal transduction is further dimonstrated using site-directed mutagenesis. The mutational effects of hlRAK on the down-stream NF-${\kappa}$ signaling shows the importance of the TRAF6 interface for signaling by IL-1.

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AN INTELLIGENT TRANSPORT MANAGEMENT SYSTEM FOCUSED ON MICROSCOPIC TRAFFIC SIGNAL CONTROL

  • Nazmi, Mohd;Takaba, Sadao
    • Proceedings of the Korea Society for Simulation Conference
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    • 2001.10a
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    • pp.77-82
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    • 2001
  • An intelligent road transport management system focused on microscopic, real-time traffic signal control is proposed. Referring to the development of those systems in Japan, extensive use of image traffic detectors observing the movement of vehicles inside intersections, and direct data exchange between the signal controllers of neighboring intersections are newly assumed. On site investigation of five intersections in Japan or in Malaysia shows the possibility of effective information provision and simple algorithm for solving heavy congestion, as well as easy installation.

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