• The KIPS Transactions:PartB
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• v.9B no.3
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• pp.351-358
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• 2002

Video compression based on DCT (Discrete Cosine Transform) has weakpoints of blocking artifacts and pixel loss when the resolution is changed. DWT (Discrete Wavelet Transform) based method can overcome such problems. In SAMCoW (Scalable Adaptive Motion Compensation Wavelet), one of wavelet based video coding algorithm, both intra frames and motion compensated error frames are encoded using EZW(Embedded Zerotree Wavelet) algorithm. However the property of wavelets transform coefficients of motion compensated error frames are different from that of still images. Signal energy is not highly concentrated in the lower bands which is true for most still image cases. Signal energy is rather evenly distributed over all frequency bands. This paper suggests a new video coding algorithm utilizing these properties. Spatial band coding which is known to be very effective for encoding images with relative1y high frequency components and not utilizing the interband coefficients correlation is applied instead of EZW to encode both intra and inter frames. In spatial band coding, the position and value of significant wavelet coefficients in each band are progressively transmitted. Unlike EZW, inter band coefficients correlations are not utilized in spatial band coding. It has been shown that spatial band coding gives better performance than EZW when applied to wavelet based video compression.

• Applied Chemistry for Engineering
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• v.33 no.2
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• pp.173-178
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• 2022

A lateral flow immunoassay (LFIA) method using carbon nanodot@silica as a signaling material was developed for analyzing the concentration of retinol-binding protein 4 (RBP4), one of the lung cancer biomarkers. Instead of antibodies mainly used as bioreceptors in nitrocellulose membranes in LFIA for protein detection, aptamers that are more economical, easy to store for a long time, and have strong affinities toward specific target proteins were used. A 5' terminal of biotin-modified aptamer specific to RBP4 was first reacted with neutravidin followed by spraying the mixture on the membrane in order to immobilize the aptamer in a porous membrane by the strong binding affinity between biotin and neutravidin. Carbon nanodot@silica nanoparticles with blue fluorescent signal covalently conjugated to the RBP4 antibody, and RBP4 were injected in a lateral flow manner on to the surface bound aptamer to form a sandwich complex. Surfactant concentrations, ionic strength, and additional blocking reagents were added to the running buffer solution to optimize the fluorescent signal off from the sandwich complex which was correlated to the concentration of RBP4. A 10 mM Tris (pH 7.4) running buffer containing 150 mM NaCl and 0.05% Tween-20 with 0.6 M ethanolamine as a blocking agent showed the optimum assay condition for carbon nanodot@silica-based LFIA. The results indicate that an aptamer, more economical and easier to store for a long time can be used as an alternative immobilizing probe for antibody in a LFIA device which can be used as a point-of-care diagnosis kit for lung cancer diseases.