• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.11
• /
• pp.1527-1533
• /
• 2001

Garole is a prolific, rare, less known and small size Indian sheep breed found in low and humid Sunderban region of West Bengal. Although information on stored Garole ram liquid semen upto 24 h is available, but there is a need to further investigate the short-term and long-term preservability of Garole ram semen for extensive utilization of this valuable germplasm by artificial insemination. The aim of the present study was to apply computer-assisted sperm analysis technique for assessing the motion characteristics of Garole ram semen stored (i) in liquid state at refrigeration temperature for short-term preservation upto 48 h and (ii) in frozen state at $-196^{\circ}C$ for long-term preservation after packaging in mini straws. Short-term preservation had a significant effect on motility (p<0.01) as the motility progressively decreased from 90.1% at 0 h to 85.5% and 73.2% after 24 and 48 h of storage, respectively. Although the decline in rapid moving sperms was also significant (p<0.01) on storage but the decrease was more pronounced at 48 h as compared to 24 h of storage period. Storage of chilled semen had also a significant effect on % linearity (p<0.05), % straightness (p<0.01), sperm velocities (p<0.01), amplitude of lateral head displacement (p<0.01) and beat frequency (pO.Ol) of spermatozoa. The replication had a significant effect for all the variables except average path and straight line velocity. However, the interactions of short-term storage and replication were non-significant for most of the variables except % of medium moving sperms, sperm velocities and beat frequency. On long-term preservation of Garole ram spermatozoa under controlled conditions the mean post-thaw recovery of 70.4 and 71.4% motile spermatozoa was achieved having 48.8 and 48.9% of rapidly motile spermatozoa, respectively in both the replicates. The effect of replication on cryopreservation was significant (p<0.05) on amplitude of lateral head displacement and beat frequency, but there was no significant effect on motility, rapidly motile spermatozoa, linearity, straightness and sperm velocities of frozen-thawed spermatozoa. It can be concluded from these results that an average 70% motility can be achieved on storage of Garole ram semen in chilled liquid state upto 48 h or in liquid nitrogen after freezing under controlled conditions in straws. However, further studies are required to evaluate the fertility of short-term and long-term preserved Garole ram semen for extensive use of this prolific sheep breed.

• Journal of Aquaculture
• /
• v.10 no.3
• /
• pp.273-279
• /
• 1997

Condition for fresh storage of tiger puffer in the liquid state were investigated in several experiments. When marine fish ringer solution and 1% NaCl were used as the diluent for the short-term preservation method, spermatozoa activity index (SAI) and survival rate showed the best result among the various diluents tested. The dilution rate for the shortterm preservation of spermatozoa was suitable between 3 and 5 times with the 1% NaCi diluent. The appropriate range of temperature for the short-term preservation showed between 0 and $5^{\circ}C$. In order to keep high SAI and survival rate of spermatozoa, antibiotic addition (800 ppm neomycin) could be suggested. These results indicated that the short-term preservation method could be employed in tiger puffer spermatozoa.

• 대한치주과학회:학술대회논문집
• /
• 2000.11a
• /
• pp.154-154
• /
• 2000

• 대한치주과학회:학술대회논문집
• /
• 1999.11a
• /
• pp.106-106
• /
• 1999

• Food Science and Preservation
• /
• v.4 no.2
• /
• pp.123-130
• /
• 1997

To develop the prediction program for quality change of Citrus unshiu during marketing, we examined the quality characteristics of Citrus unshiu stored at experimental refrigerator set to 4, 8, 12 and 16$^{\circ}C$ for 2 months. According to the storage temperature the changes of quality characteristics were different respectively, but it was most severe during 16$^{\circ}C$ storage. Activation energy and Q10 value were 6683.16 cal/mol K and 1.53 respectively. The determination coefficient of regression equation of pH, acidity and vitamin C by surface response analysis were over 0.85. Using these regression equation, we developed the prediction program for the change of pH, acidity and vitamin C contents. The calculated values and experimental values of pH, acidity and vitamin C contents for short-term storage of Citrus unshiu were coincided well.

• Journal of Environmental Impact Assessment
• /
• v.21 no.1
• /
• pp.185-199
• /
• 2012

Ecotourism has been attracting much attention as an example industry that can vitalize local economy markets and preserve natural resources. Implementation of 'Ecotourism Designation System' is under consideration to provide ecotourism products that can both emphasize the original meanings of ecotourism and ensure stronger trust from customers than ever before. This research introduced the direction, principles, subjects, and assessment indicators by subjects in terms of implementing the system and proposed fundamentals for both designation assignment and score calculation. In the face of implementing such a system in Korea, the investigation sought subjects whose target areas and programs are in need of examination. For assessment criteria by subjects, six assessment indicators were selected in the end for ecotourism sites, including: (1) the value of natural/ecological preservation (2) facility's ecological design (3) understanding both the environment and local culture (4) systems connecting local communities for cooperation (5) long/short term preservation and maintenance strategies/plans (6) willingness to further develop ecotourism and cooperative platform. In addition, seven assessment indicators were selected for program assignments, including: (1) the value of natural/ecological preservation (2) minimization of environmental effects (3) expansion of environmental understanding (4) sharing the local culture (5) vitalizing local connections and economical activities among communities (6) securing and maintaining professionality among human resources (7) odds of continuation, creativity, and aptness to improve programs. After considering the pros and cons of both categorizing system and yes/no system, implementation of categorizing system for the short term and yes/no for the long term were suggested, followed by additional suggestions of a score calculation system for each system. The official implementation of the 'Ecotourism Designation System' is scheduled for launch in 2013 after the trial operation in 2012. The assessment indicators and fundamentals suggested in this research, considering judgment, must be improved via systematic and practical methods through the trial operation.

• Food Science and Preservation
• /
• v.22 no.5
• /
• pp.623-628
• /
• 2015

Fresh cut vegetables provide convenience and rapidity to consumers. However, they have a weakness with respect to their short shelf-life due to browning and quality degradation via increased respiration. To overcome this problem, the effect of packaging film on the short-term storage of cut kimchi cabbages was investigated. Polypropylene (PP), oriented polypropylene (OPP), and low-density polyethylene (LDPE) films were used as packaging film, and cut kimchi cabbages were stored in the packaging films at $5^{\circ}C$ for 4 weeks. PP film packaging showed the least weight loss and soluble solids loss after 4 weeks. Titratable acidity increased during storage for all samples, however, the increase rate of titratable acidity in PP and OPP film packaging decreased, which was lower than that of LDPE film packaging. Color values decreased over time during storage. In appearance, PP film packaging was better than other films due to their high transparency. In a sensory test, there was no statistical difference among samples. Taken together, the transparent PP film packaging was more effective for short-term storage of cut kimchi cabbages. Thus, this study provides useful information for the selection of packaging materials for cut kimchi cabbage marketing.

• Korean Journal of Animal Reproduction
• /
• v.16 no.4
• /
• pp.301-310
• /
• 1993

This experiment was performed to develop simple practical methods for short term preservation of rabbit embryos. A total of 55 cross bred does were superovulated by intramuscular injection of PMSG and HCG. Embryos were recovered at 25~30 hrs, 60~65 hrs and 80~85 hrs after mating and selected by morphological examination. Four cell stage, morulae and blastocyst embryos were stored in PBS enrich with 1, 10, 20 and 40% heat-treated FCS at 4, 20, 30 and 37$^{\circ}C$, respectively. Embryos were examined morphologically at 24, 48 and 72 hrs following storage. The result obtained in this experiment were summarized as follows: The superovulation was induced by PMSG 200 IU and HCG 100 IU. The average number of ovulation points and embryos recovered by collection time were 19.0, 15.6(25~30 hr), 17.3, 13.5(60~65 hr) and 19.2, 14.4(80~85 hr), respectively. And recovery rates of embryos recovered at 25~30 hr, 60~65 hr and 80~85 hr after mating were 62.8%(4 cell), 84.7%(morulae) and 79.6%(blastocyst), respectively. On the other hand, the average number of ovulation points collected by the no, of operations for the repeated collection was 17.3(60~65 hr), 19.2(80~85 hr) in 1st and 9.4(60~65 hr), 10.6(80~85 hr) in 2nd surgery, respectively. There was a significant decrease(P<0.05) in the number of ovulation points the 2nd surgery as compared to the 1st surgery. All of the 4-cell stage embryos stored at 4$^{\circ}C$ for 48 hrs showed the same morphology throught the storage period, on the contrary, 4-cell stage embryos stored at 2$0^{\circ}C$ and 3$0^{\circ}C$ for 48 hrs showed degeneration embryos and stored at 37$^{\circ}C$ for 24 hrs showed degeneration embryos. Morulae and blastodcyst stored at 4, 20, 30 and 37$^{\circ}C$ for 24 hrs showed degeneration embryos. All of the blastocyst stored at 37$^{\circ}C$ for 72 hrs showed degeneration embryos.

• The Korean Journal of Archival Studies
• /
• no.79
• /
• pp.343-387
• /
• 2024

Electronic records, characterized by their inherent volatility and instability, necessitate sustainable preservation measures to ensure their long-term accessibility. The National Archives of Korea has instituted a selection system for preservation formats tailored predominantly for document-type electronic records. However, this system falls short in accommodating other record types such as audiovisual records. This study endeavors to broaden the applicability of the existing system, with a concentrated focus on image-type electronic records, and to formulate foundational guidelines for their long-term preservation. In South Korea, image-type electronic records rank as the second most prevalent category following document-type. The image-type electronic records are the most basic form of audio-visual records, and research on this lays the foundation for future discussions on other audio-visual records. Consequently, this research has led to the development of a selection system for preservation formats specifically for image-type electronic records. This system is designed to facilitate the prompt and efficient evaluation of preservation format suitability, even in the context of emerging image formats. The efficacy of this system was validated through its application to extant image formats, resulting in the selection of TIFF, JFIF, and PNG as the optimal preservation formats. The outcomes of this study offer valuable insights and practical reference points for future preservation format evaluations within the field of electronic record management.

• Korean Journal of Animal Reproduction
• /
• v.14 no.3
• /
• pp.199-204
• /
• 1990

Equine semen was analysed for its survival after storage under different conditions. Total 12 ejaculates from 2 Thoroughbred were analysed for general characterisitcs and preservation at low temperature. The sperm fraction, concentration, nd the rate of motile spermatozoa were 57.91ml per ejaculate, 2.18$\times$108/ml and 74.1%, respectively. The survival rate of spermatozoa was highest when diluted semen with E-Z Mixin was stored at 7~8$^{\circ}C$. The optimum survival rate(>54%) can be obtained upto 24h at 7~8$^{\circ}C$. However only 10% spermatozoa survived after 5h storage at 7~8$^{\circ}C$ without use of E-Z Mixin. Other ranges of temperature(15$^{\circ}C$ and room temperature) gave less survival rates(<25%). These results indicate that the extender could be used as a basic solution for the preservation of equine spermatozoa at low temperature. It also provides a practical method for short-term storage of collected equine semen in a simple manner.