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Assay of ${\beta}$-Glucosidase Activity of Bifidobacteria and the Hydrolysis of Isoflavone Glycosides by Bifidobacterium sp. Int-57 in Soymilk Fermentation

  • Jeon, Ki-Suk;Ji, Geun-Eog;Hwang, In-Kyeong
    • Journal of Microbiology and Biotechnology
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    • v.12 no.1
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    • pp.8-13
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    • 2002
  • The isoflavone glycosides are hydrolyzed by ${\beta}$-glucosidase from gut microbes to the bioactive aglycones. However, the specific bacteria from the human intestinal tract that are involved in the metabolism of these compounds are not known. This study was undertaken to develop a fermented soymilk which converts isoflavones to the more bioactive aglycones form using a Bifidobacterium strain. The ${\beta}$-glucosidase activity of 15 Bifidobacterium strains were measured during cell growth. Among them, Bifidobacterium sp. Int-57 was selected for this study, because it has the highest ${\beta}$-glucosidase activity. Growth, acid development, ${\beta}$-glucosidase activity, and the hydrolysis of daidzin and genistin were investigated in four soymilks inoculated with Bifidobacterium sp. Int-57. After 12 h of fermentation, the counts of viable Bifidobacterium sp. Int-57 in all the soymilks reached a level of more than $10^8$ cfu/ml, which was then maintained. The pH of soymilks started to decrease rapidly after 6 h of fermentation and leveled off after 18 h. The titratable acidity of BL# 1 soymilk, BL#2 soymilk, and JP#l soymilk increased from 0.18 to 1.21, 1.15, and $1.08\%$ over the fermentation period, respectively. After 24 h of fermentation, the $\beta$-glucosidase activity in BL#1 soymilk, BL#2 soymilk, JP#l soymilk, and JP#2 soymilk increased to 59.528, 40.643, 70.844, and 56.962 mU/ml, respectively. The isoflavone glycosides, daidzin and genistin, in soymilks were hydrolyzed completely in the relatively short fermentation time of 18 h. These results show that Bifidobacterium sp. Int-57 can be used as a potential starter culture for developing fermented soymilk which has completely hydrolyzed isoflavone glycosides.

A Study on the Difference of Cold-heat Patterns between Health and Mibyeong Group (건강군과 미병군의 한열지표 차이에 관한 연구)

  • Kim, Sujung;Lee, Siwoo;Lee, Youngseop
    • Journal of Society of Preventive Korean Medicine
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    • v.21 no.1
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    • pp.49-56
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    • 2017
  • Objectives : In this study, we diagnosed Mibyeong group of adult by taking into Mibyeong Index consideration, and identified the correlation of a Mibyeong group with cold-heat pattern and life quality of them. Methods : The questionnaires were collected by Gallup Korea professional surveyor through face to face interviews. To analyze the differences between health and mibyeong group, we used the descriptive statistics, Pearson's correlation, ANOVA. And multinomial logistic regression was used to generate the odds ratios (ORs) and 95 % confidence interval (CI) for the differences between health and mibyeong group. Results and Conclusions : The gender composition of respondents in this study that there 545 male (49.5%) and 555 female (50.5%). The score of both cold pattern(health: $21.33{\pm}4.25$, MI 1: $22.43{\pm}4.29$, MI 2: $24.09{\pm}5.03$; post hoc test, p <0.001) and heat pattern(health: $18.4{\pm}4.01$, MI 1: $19.48{\pm}4.10$ MI 2: $19.88{\pm}4.81$; post hoc test, p <0.001) in mibyeong group is higher than the score health group. And, these result have no relevance to age. The score of both Physical component summary (PCS) and Mental component summary (MCS) in health group is higher than the score mibyeong group. Cold-heat pattern and quality of life vary significantly according to health status. This results suggest the analysis of cold-heat pattern and quality of life by health status could provide the setting of direction to promote public health depending on health status.

General Health Related Quality of Life and Associated Factors among Prostate Cancer Patients in Two Tertiary Medical Centers in Kuala Lumpur, Malaysia: A Cross-sectional Study

  • Isa, Mohamad Rodi;Ming, Moy Foong;Razack, Azad Hassan Abdul;Zainuddin, Zulkifli Mohd;Zainal, Nur Zuraida
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.12
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    • pp.5999-6004
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    • 2012
  • Measurement of quality of life among prostate cancer patients helps the health care providers to understand the impact of the disease in the patients' own perspective. The main aim of this study is to measure the quality of life among prostate cancer patients at University Malaya Medical Center (UMMC) and Universiti Kebangsaan Malaysia Medical Centre (UKMMC) and to ascertain the association factors for physical coefficient summary (PCS) and mental coefficient summary (MCS). A hospital based, cross sectional study using the Short Form-36 (SF-36) questionnaire was conducted over a period of 6 months. A total of 193 respondents were recruited. Their total quality of life score was $70.1{\pm}14.7$ and the PCS score was lower compared to MCS. The factors associated for PCS were: age, living partner, renal problem, urinary problem of intermittency, dysuria and hematuria. Factors associated for MCS were: age, living partner, renal problem, presenting prostatic specific antigen and urinary problem of intermittency and dysuria. Our prostate cancer patients had moderate quality of life in the physical health components but their mental health was less affected.

Synthesis and Crystal Structure of a New Pentanary Thiophosphate, K0.5Ag0.5Nb2PS10

  • Dong, Yong-Kwan;Kim, Sang-Rok;Yun, Ho-Seop;Lim, Han-Jo
    • Bulletin of the Korean Chemical Society
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    • v.26 no.2
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    • pp.309-311
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    • 2005
  • Single crystals of the new pentanary thiophosphate, $K_{0.5}Ag_{0.5}Nb_2PS_{10}$ has been prepared through reactions of the elements with halide fluxes. The structure of $K_{0.5}Ag_{0.5}Nb_2PS_{10}$ has been analyzed by single crystal X-ray diffraction technique. The structure of $K_{0.5}Ag_{0.5}Nb_2PS_{10}$ is made up of one-dimensional ${^{\infty}_1}[Nb_2PS_{10}]$ chains along the [001] direction and these chains are separated from one another by $Ag^+$ and disordered $K^+$ ions. This chain is basically built up from bicapped trigonal prismatic [Nb2S12] units and tetrahedral [PS4] groups. The [$Nb_2S_{12}$] units are connected together to form the linear chain, ${^{\infty}_1}[Nb_2S_9]$ by sharing the S-S prism edge. Short (2.885(2) $\AA$ or 2.888(2) $\AA$) and long (3.743(1) $\AA$) Nb-Nb distances are alternating along the chain, and the $S_2{^2-}]\;and\;S^{2-}$anionic species are observed. The distorted octahedral coordination around the $Ag^+$ ion can be described as [2+4] bonding interaction.

Early versus Delayed Surgery for Spinal Epidural Abscess : Clinical Outcome and Health-Related Quality of Life

  • Behmanesh, Bedjan;Gessler, Florian;Quick-Weller, Johanna;Dubinski, Daniel;Konczalla, Juergen;Seifert, Volker;Setzer, Matthias;Weise, Lutz
    • Journal of Korean Neurosurgical Society
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    • v.63 no.6
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    • pp.757-766
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    • 2020
  • Objective : Spinal epidural abscess (SEA) is a severe and life-threatening disease. Although commonly performed, the effect of timing in surgical treatment on patient outcome is still unclear. With this study, we aim to provide evidence for early surgical treatment in patients with SEA. Methods : Patients treated for SEA in the authors' department between 2007 and 2016 were included for analysis and retrospectively analyzed for basic clinical parameters and outcome. Pre- and postoperative neurological status were assessed using the American Spinal Injury Association Impairment Scale (AIS). The self-reported quality of life (QOL) based on the Short-Form Health Survey 36 (SF-36) was assessed prospectively. Surgery was defined as "early", when performed within 12 hours after admission and "late" when performed thereafter. Conservative therapy was preferred and recommend in patients without neurological deficits and in patients denying surgical intervention. Results : One hundred and twenty-three patients were included in this study. Forty-nine patients (39.8%) underwent early, 47 patients (38.2%) delayed surgery and 27 (21.9%) conservative therapy. No significant differences were observed regarding mean age, sex, diabetes, prior history of spinal infection, and bony destruction. Patients undergoing early surgery revealed a significant better clinical outcome before discharge than patients undergoing late surgery (p=0.001) and conservative therapy. QOL based on SF-36 were significantly better in the early surgery cohort in two of four physical items (physical functioning and bodily pain) and in one of four psychological items (role limitation) after a mean follow-up period of 58 months. Readmission to the hospital and failure of conservative therapy were observed more often in patients undergoing conservative therapy. Conclusion : Our data on both clinical outcome and QOL provide evidence for early surgery within 12 hours after admission in patients with SEA.

Relationships between compliance and health-related quality of life in patients with hemodialysis (혈액투석환자의 이행과 건강관련 삶의 질 간의 관계)

  • Cha, Jieun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.10
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    • pp.6495-6503
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    • 2015
  • The purpose of the study was to evaluate patients' compliance with the hemodialysis and to investigate relationships between compliance, physiological parameters, and health-related quality of life. Data were collected from 220 patients at 27 local hemodialysis clinics. Compliance was measured with Sick-role Behavioral Compliance including interdialytic weight gain, serum potassium, and phosphorus. Health-related quality of life was assessed using Medical Outcomes Study Short Form-12. Data were analyzed using descriptive statistics, t-tests, ANOVA, and Pearson correlation. Total compliance scored 2.92 out of 4 points on average. Among fifteen items, 'I keep on my dialysis schedule(time and date)' was the highest score. There were differences in the scores for compliance according to age, marital status, and dialysis period. Statistically significant correlations were found between four compliance items(medication, infection control, sleep, eating vegetable and fruit) and health-related quality of life. The results of the study indicate that a patient-centered approach would be helpful to improve quality of life in patients with hemodialysis. Healthcare providers need to understand the patients' perspectives by identifying what is important to patients and taking patient values and priorities into account.

A Research on the Actual Conditions of Library Practice Education in Korea (한국에 있어서 사서실습교육의 실태 조사연구)

  • Koo Bon-Young
    • Journal of the Korean Society for Library and Information Science
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    • v.10
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    • pp.3-37
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    • 1983
  • 1. Library practice education in Korea is in the form of field experience, that is, Practicum, which is one type of field experience. 2. The practicum is a short-term work experience guided by the academic institutions. (2 to 6 weeks of full time employment in a library, or one to two semesters of approximately 10 to 12 hours weekly) 3. Field work is largely accepted as a required subject. 4. Field work is usually carried out during the first semester of senior year. (or of sophomore year in junior college) 5. Libraries in other universities (or in other institutions) can beused for a student's field work. But the current tendency is to use his or her own university's library. 6. Most universities give one credit for 2 weeks' field work. 7. The method of evaluation is strict: A or 'excellent' (above 90), B or 'good' (above 80), C or 'fair' (above 70), D or 'bad' (above 60). 8. The expense of field work is usually included in the tuition fees. However, some think that an additional fees should be collected in the case of special practice. 9. Respondents to my survey say that the field experience is necessary so that professional librarians can connect theory and practice. 10. They also indicate that there are some problems in library practice education: administrative difficulties in the department chairman's office, the burden on professors in the library being used, and the limited number of libraries in which the field work is practicable. 11. The Department of Library Science is responsible primarily for providing faculty supervision over the students, and secondarily for the preparation of evaluation cards and field work reports. 12. Most of the faculty respondents use evaluation cards for the field work, some of which serve as grade sheets, some as field work diaries, and some as field work reports.

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The Association between Mibyeong Symptoms and Quality of Life (미병 증상과 삶의 질 연관성 연구)

  • Park, Kihyun;Lee, Youngseop;Jin, Hee-Jeong
    • Journal of Society of Preventive Korean Medicine
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    • v.21 no.2
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    • pp.35-44
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    • 2017
  • Objectives : The purpose of this study was to investigate the association between quality of life and Mibyeong index of abnormal symptoms by the Mibyeong state. Methods : A total of 1,100 people were recruited into this study and were collected by specialized research company. The data were collected by personal information using MBI(Mibyeong Index) and SF-12 (Short form-12) and were analyzed with SPSS (version 21.0) computer program, and included Pearson's chi-square test, ANOVA, Pearson's correlation coefficient, and multiple regression analysis. Results : The Mibyeong Index which represents abnormal symptom type showed significant difference for Mibyeong state (p<.001). In Total subject, fatigue, pain, low sleep quality and indigestion were found to be significant predictors of PCS(Physical Component Summary). In Healthy group, pain was found to be significant predictor of PCS. In MI 1 group, fatigue, pain, indigestion and anxiety were found to be significant predictors of PCS. In MI 2 group, fatigue, pain and low sleep quality were found to be significant predictors of PCS. In Total subject, fatigue, depression, anger and anxiety were found to be significant predictors of MCS (Mental Component Summary). In Healthy group, indigestion and anxiety were found to be significant predictors of MCS. In MI 1 group, depression and anger were found to be significant predictors of MCS. In MI 2 group, fatigue and depression were found to be significant predictors of MCS. Conclusions : The relationship between Quality of life and MBI changes according to Mibyeong state. We expect that this result could be contribute to health management of people in Mibyeong state.

Mother-Child Emotional Availability Mediating the Effects of Maternal Psychological Well-being and Child's Cognitive Competence on Child Behavior Problems

  • Kang, Min-Ju
    • International Journal of Human Ecology
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    • v.12 no.2
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    • pp.95-107
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    • 2011
  • Recent intervention studies document that mother-child dyads with higher levels of Emotional Availability (EA) report fewer child behavior problems than dyads with lower EA. This study examines possible mechanisms that lead to this result by looking at the parent-child micro-system as a whole, with multi-dimensional relationships that include individual differences in the child's cognitive level, parental stress and parent-child interaction. A total 67 children ($1{\frac{1}{2}}$ to $5\;{\frac{1}{2}}$ years of age) and their mothers were videotaped during 30-min play interactions. Interactions were coded using the Emotional Availability (EA) Scales (Biringen, Robinson, & Emde, 1998). Mothers completed Parenting Stress Index-Short Form, Child Behavior Checklist/$1\;{\frac{1}{2}}$ - 5, and the Ages Stages Questionnaire. The findings showed that mothers with higher levels of parenting stress were more likely to be intrusive, hostile, insensitive, and had a tendency to do less structuring in play. The children of stressed and depressed mothers demonstrated less involvement and responsiveness towards their mothers. Children who have higher dyadic EA scores experienced fewer externalizing and internalizing problems. SEM analyses results showed a mediation effect of EA on the association between maternal psychological well-being and child behavior problems. Fewer deficits in child communication skills and problem solving skills that were related with lower parenting stress and depression were associated with higher maternal non-intrusiveness. Higher non-intrusiveness was related to less internalizing and externalizing problems that indicated the indirect effect of child cognitive competence. Possible interpretations and implications of the study findings are discussed.

AN ANALYSIS OF INFRARED IMAGES OF JUPITER IMPACTED BY P/SHOEMAKER-LEVY 9

  • KIM YONG HA;SUNG KIYUN;KIM SANG JOON;COCHRAN W. D.;LESTER D. F.;TRAFTON L.;CLARK B. E.
    • Journal of The Korean Astronomical Society
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    • v.29 no.2
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    • pp.245-253
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    • 1996
  • We have analyzed infrared (IR) images of Jupiter which was observed at the McDonald Observatory, Texas, U.S.A., during the P/SHoemaker-LEvy 9 (SL9) impact period and about one week after the last impact. The IR images were obtained on the 2.7m telescope using a NICMOS array with filters to isolate the $1.5{\mu}m\;NH_3\; band,\;the\;2.3{\mu}m\;CH_4\;band,\;the\;2.12{\mu}m\;H_2\;S(0)$ pressure-induced absorption, and the continua at $1.58{\mu}m\;and\;2.0{\mu}m$ (short K-band). All images except those with the $1.58{\mu}m$ continuum filter show bright impact sites against the relatively dark Jovian disk near the impact latitude of about $45^{\circ}$ S. This implies that dusts originated from the impacts reflect the solar radiation at high altitudes before absorbed by stratospheric $CH_4,\;NH_3 \;or\;H_2$. The impact sites observed with the $2.3{\mu}m$ filter are conspicuously bright against a very dark background. The morphology of impact sites, G, L, and H at 2.3 and $2.12{\mu}m$ filters shows clearly an asymmetric structure toward the incident direction of the comet fragments, in agreement with the studies of visible impact images obtained with the Hubble Space Telescope. Comparisons of reflectances of G, L, and H sites with simple radiative transfer models suggest that optically thick dust layers were formed at high altitudes at which methane absorption attenuates incoming sunlight only by about $1\%$. The dust layers in these sites seem to form at about the same altitude regardless of the magnitude of the impacts, but they appear to descend gradually after the impacts. The dust layers have optical depths of 2-5, according to the models.

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