• 한국자원식물학회지
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• 제27권2호
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• pp.183-187
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• 2014

헛개나무(Hovenia dulcis var. koreana Nakai, Korea raisin tree)는 동아시아가 원산지이며, 식 약용 수종으로서 밀원수종으로도 가치가 증대되고 있는 수종이다. 본 연구는 헛개나무의 절접에 의한 접수의 채취시기와 연령 및 비닐하우스 설치에 따른 접목활착율의 효과를 조사하는데 목적이 있다. 헛개나무 신품종은 절접에 의한 접목 결과 클론간 유의적인 차이가 인정되지 않았다. 온대 북부 권역에서는 휴먼기인 춘분 전에 접수를 채취하는 것이 가장 적합하며, 비닐하우스를 설치하는 경우에는 86% 이상의 높은 접목 활착율을 얻을 수 있다. 접수 연령은 1년지가 가장 적합하며 접목 후 비닐터널을 설치하여 접목상을 관리하면 80% 이상의 높은 접목활착율을 얻을 수 있는 것으로 나타났다.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2018년도 전력전자학술대회
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• pp.286-287
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• 2018

Recently, the development of renewable energy using solar energy is drawing attention. One of these PV systems, the Dual buck Inverter, is one of the topologies used in conjunction with the system, characterized by high efficiency and shoot-through reduction. However, current distortion appears severely in grid connection. So, in this paper, the method of compensation through PR controller was verified through simulation as an analysis of current distortion components and a control method to improve them.

• 약학회지
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• 제60권3호
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• pp.112-117
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• 2016

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of caffeine in forensic aqueous sample. The centrifuged sample ($100{\mu}l$) was diluted 50-fold with distilled water. The diluted sample ($400{\mu}l$) was then diluted further with $200{\mu}l$ of 0.1% formic acid solution and $400{\mu}l$ of acetonitrile containing 500 ng of caffeine-(3-methyl-$^{13}C_3$) prior to LC-MS/MS analysis. The mobile phase was composed of 0.1% formic acid in distilled water (A) and acetonitrile (B). Chromatographic separation was performed by using a Zorbax SB-C18 ($100mm{\times}2.1mm$ i.d., $3.5{\mu}m$) column and caffeine was eluted within 1.1 min. Linear least-squares regression with a 1/x weighting factor was used to generate a calibration curve with the coefficients of determination ($r^2=0.9983$). The lower limit of quantification was $25ng/ml$ for the analyte. The process efficiency was 98.6~100.1%. Intra- and inter-day precisions were not more than 2.1% and 1.7%, while intra- and inter-day accuracies were ranged from -6.8 to 4.5%, respectively. The suitability of the method was examined by analyzing unknown forensic aqueous samples.

• Plant Biotechnology Reports
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• 제4권2호
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• pp.157-163
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• 2010

A protocol for in vitro propagation from pseudobulb sections of Lycaste armomatica (Graham ex Hook) Lindl., an ornamental and fragrant orchid, was developed. The effect of four cytokinins: kinetin (K), metatopolin (mT), $N^6$-benzyladenine (BA), and thidiazuron (TDZ), in equimolar concentrations, was investigated. Shoot formation from apical and basal pseudobulb sections was obtained in all treatments. A few medial sections cultured in media supplemented with BA formed protocorm-like bodies. Shoot formation was greater from the basal section than the apical, and mainly occurred in explants cultured in media containing TDZ. The highest average numbers of shoots per explant were achieved from basal sections cultured in media supplemented with TDZ at 4.4, 8.87 and 2.2 ${\mu}M$, forming on average 9.9, 8.6 and 7.3 shoots per explant, respectively. Since the medial pseudobulb section was the worst explant for propagation of L. aromatica, we recommend that pseudobulbs be divided into two sections; the basal half should be cultured in MS medium supplemented with TDZ at 4.4 ${\mu}M$ and the apical half with TDZ at 2.2 ${\mu}M$. Subculturing individual shoots in MS medium without plant growth regulators allows further development and rooting. A survival rate of more than 90% under greenhouse conditions was achieved. This research represents a direct contribution to the conservation and sustainable use of this valuable natural resource.

• Molecules and Cells
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• 제21권1호
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• pp.147-152
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• 2006

Most plant organs develop from meristems. Rice FON1, which is an ortholog of Clv1, regulates stem cell proliferation and organ initiation. The point mutations, fon1-1 and fon1-2, disrupt meristem balance, resulting in alteration of floral organ numbers and the architecture of primary rachis branches. In this study, we identified two knockout alleles, fon1-3 and fon1-4, generated by T-DNA and Tos17 insertion, respectively. Unlike the previously isolated point mutants, the null mutants have alterations not only of the reproductive organs but also of vegetative tissues, producing fewer tillers and secondary rachis branches. The mutant plants are semi-dwarfs due to delayed leaf emergence, and leaf senescence is delayed. SEM analysis showed that the shoot apical meristems of fon1-3 mutants are enlarged. These results indicate that FON1 controls vegetative as well as reproductive development by regulating meristem size.

• Journal of Crop Science and Biotechnology
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• 제10권2호
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• pp.92-97
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• 2007

Antibiotics used for suppressing Agrobacterium in plant transformation procedure might have negligible effects on plant tissues and regeneration. The effects of antibiotics on growth suppression of Agrobacterium and plant regeneration were investigated for enhancing Agrobacterium-mediated transformation using wheat mature embryos. Antibiotics tested, except carbenicillin, were able to suppress that embryos were coated with a layer of Agrobacterium cells in callus induction medium. Agrobacterium growth was suppressed minimally at 50 mg/l of timentin, while cefotaxime and clavamox were completely suppressed at relative high concentration of 250 mg/l. In the treatment of carbenicillin, initiation of growth suppression of Agrobacterium occurred at 750 mg/l of concentration because Agrobacterium KYRT1 contains the carbenicillin resistant gene. In Agrobacterium inoculation, effects of antibiotics were significantly different on the rate of callus induction and shoot formation. Almost embryos were induced calli at 50 mg/l of timentin whereas callus induction rate was achieved above 90% at 100 mg/l and 250 mg/l of cefotaxime and clavamox, respectively. Shoot formation rate was higher in the treatment of timentin than that of cefotaxime and clavamox at 500 mg/l of concentration, respectively. Timentin can be used as a good antibiotics in Agrobacterium-mediated wheat transformation.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2005년도 추계학술대회 학술발표 논문집 제15권 제2호
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• pp.317-320
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• 2005

본 논문에서는 퍼지 로직을 이용하여 로봇의 여러 환경변수에 따라 로봇들의 행동을 적절히 선택하는 알고리즘을 제시한다. 전략 및 전술 알고리즘으로 많이 알려진 Modular Q-학습 알고리즘은 개체의 수에 따른 상태수를 지수 함수적으로 증가시킬 뿐만 아니라, 로봇이 협력하기 위해 중재자모듈이라는 별도의 알고리즘을 필요로 한다. 그러나 앞으로 제시하는 로봇 행동의 퍼지 적정성을 고려한 로봇축구 전략 및 전술 알고리즘은 환경 변수에 따라 로봇 행동의 적절성을 퍼지 로직을 통하여 얻어내게 하였으며, 이를 이용함으로써 다수 로봇의 상호작용도 고려할 수 있게 하였다.

• 농업과학연구
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• 제37권2호
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• pp.171-175
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• 2010

This study was carried out to find the effect on growing media and proper time for water supply in rice seedling stages. Plant height of rice seedling for SIPAN-BRL among current several growing medias was higher in Chuchungbyeo than Ilpumbyeo, and that of root length was higher in check among used growing medias. Fresh and dry weight of shoot and root of two rice cultivar. Water time to absorption on each treatment of growing media concentration took similarly in SIPAN-BNH and check were similar. But 500 minutes did in 100ppm and did 370 and 470minute in 300 and 500ppm, respectively. Wilting time of rice seedling was appeared highly at 300ppm of SIPAN-BRL in Chuchungbyeo and 100ppm of SIPAN-BRL in Ilpumbyeo, while it prolonged at 300ppm in Chuchungbyeo and 500ppm in Ilpumbyeo, respectively. Water content at wilting stage was appeared highly at SIPAN-BRL 500ppm in both Chuchungbyo and Ilpumbyeo, while SIPAN-BNL was high in 300ppm of Chuchung-byeo and Ilpumbyeo, respectively. Plant and root length of SIPAN-BNL treated with 300ppm in 10day-rice seedling were highly appeared, while dry weight of shoot was high at 300ppm SIPAN-BNL.

• 농업과학연구
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• 제19권2호
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• pp.153-160
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• 1992

잔디상토로서 제지슬러지와 연탄재의 이용 가능성을 구명하기 위해 들잔디(Zoysia japonica Steud.)를 공시재료로하여 모래와 슬러지, 연탄재와 슬러지의 배합비율을 각각 3:1, 2:1, 1:1, 1:2, 1:3(V/V)으로 혼합하여 잔디의 생육과 적정 배합비율 등을 조사한 결과는 아래와 같다. 1. 활성슬러지는 대조구에 비해 초장, 생체중, 건물중을 2배이상 증가시켰으며, 모래와의 혼합비율간에는 슬러지가 67%혼합된 1:2구에서 가장 생육이 좋았다. 2. 단위면적당 shoot의 밀도는 모래와 활성슬러지의 혼합비율간에 큰 차이가 없이 대조구에 비해 모든 처리구에서 많았으며, 엽록소함량은 활성슬러지가 적게 혼합된 3:1, 2:1구에서는 대조구와 차이가 없었으나 슬러지의 함유량이 50%이상인 1:1, 1:2, 1:3구에서는 증가를 보였다. 3. 생슬러지는 대조구에 비해 잔디 생육이 저조하고 슬러지가 많이 함유된 1:3구에서는 파종잔디의 생육이 어려웠으나 엽록소함량은 큰 차이가 없었다. 그러나 영양번식을 하였을 경우 분얼증가는 대조구와 차이가 없거나 약간 저조한 경향을 보였다. 4. 연탄재와 활성슬러지의 혼합구는 모래 혼합구에 비해 잔디의 초장이 다소 적었으나 shoot의 밀도는 비슷하였고, 대조구에 비해 초장, 단위면적당 밀도, 생체중, 건물중, 분얼수가 월등히 증가하였다. 5. 연탄재와 생슬러지의 혼합토양은 파종된 잔디의 생육에는 불량하였으나 영양번식 잔디의 분얼을 대조구보다 증가시켰다.

• Journal of Plant Biotechnology
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• 제40권3호
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• pp.147-155
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• 2013

이 실험에서 우리는 더덕의 기내 소비대근형성에 의한 대량생산을 처음으로 보고하였다. 첫번째로, 다경지유도를 위한 싸이토키닌의 효과를 실험하였다. 2.0 $mg{\cdot}L^{-1}$ kinetin 처리는 줄기유도비율(19.1%)뿐만 아니라 줄기의 길이신장(17.1 mm)이 가장 높았다. 두번째로, 절간절편에서 유도되는 기내 소비대근형성에서 탄소급원의 효과를 연구하였다. 비대근의 직경은 1/2 MS배지에 145.9 mM Suc첨가시 증가하였다. 조직학적 분석은 비대근 내에 전분입자가 포함된 유세포의 수가 증가하였다. 게다가 세근은 비대근과 달리 관다발이 피층내에 흩어져 존재하였다. 세번째로, 소비대근의 보전과 발아 촉진을 위하여 $4^{\circ}C$ 저온저장고에 9달 동안 저장 후 이것을 인공상토로 이식한 결과 생존율 75%, 발아율 70%라는 결과를 보였다. 이러한 결과 더덕의 대량 번식은 기내 소비대근 형성에 의해 얻을 수 있고 이러한 방법은 우수클론의 번식 뿐만 아니라 더덕 유전자원 보존을 위해 제공될 수 있을 것으로 사료된다.