• Horticultural Science & Technology
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• v.33 no.6
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• pp.877-882
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• 2015

This study was carried out treatment of polyethylene glycol (PEG) in order to increase of survival rate of acclimated plants of strawberry's in vitro plantlets through bioreactor culture. We used PEG with molecular weight 6000 (PEG 6000) in this study. Concentration of PEG is non-treatment, 5, 10, 15, and $20g{\cdot}L^{-1}$ each bioreactor. $5g{\cdot}L^{-1}$ of PEG was treated at $1^{st}$, $2^{nd}$, $3^{rd}$, $4^{th}$, and $5^{th}$ week during culture. We investigated growth characteristics of in vitro plantlets after 6 weeks cultivation. Growth amount of all PEG treatment decreased as compared to non-treatment. The more concentration increased, the more plant growth decreased. In $5g{\cdot}L^{-1}$ of PEG, shoot length was shorter than non-treatment that shoot length was 7.9 cm and especially fresh weight that is 1.6 g was more decrease than non-treatment. Shoot length was ranged 3.0-3.9 cm at $1^{st}$ week treatment to $4^{th}$ week treatment of $5g{\cdot}L^{-1}$ PEG. The shoot length was not significant by 5.3 cm at $5^{th}$ week treatment. The survival rate was improved 5.4% at the treatment of $4^{th}$ week and was improved 8.7% at the $5^{th}$ week as compared to non-treatment. In order to improve of survival rate of strawberry' in vitro plantlets through bioreactor culture, the method is suitable that adding $5g{\cdot}L^{-1}$ of PEG in the medium and $5^{th}$ week's treatment is suitable.

• Korean Journal of Plant Resources
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• v.26 no.4
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• pp.503-510
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• 2013

This study was conducted to determine the optimum intensity of root pruning and the initial seedling size of 1-year-old Quercus serrata for producing 2-year-old healthy seedlings. Large( > 26 cm hight) and small size ( < 24 cm hight) seedlings were pruned their root by the length of 5, 10, and 15cm and then transplanted on nursery. Stem height, root-collar diameter(RCD), height to RCD ratio(H/D ratio), shoot dry weight(DW) and root development were measured during first growing season after transplanting. Seedling size and pruning intensity influenced on shoot growth such as height, RCD, H/D ratio and DW after transplanting. In addition, the development of lateral roots such as number, length, DW and diameter were affected by the seedling size. Taproot pruned by 15cm was shown excellent above-ground growth in large seedling group, but pruned by 10 and 15cm showed respectively better shoot growth in small seedling group. Large seedlings increased their above-ground growth, taproot regrowth, and number of lateral roots more than those of the small seedlings. But the length and DW of lateral roots were increased in the small seedlings. In conclusion, in order to produce high quality 2-year-old seedlings, it is important to produce large size of Q. serrata seedlings. Also root pruning length of 1-year-old Q. serrata was reasonable on 15 cm in taller than 26 cm or 10cm in less than the height.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.1B
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• pp.101-105
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• 2010

Sunflower (Sunking4505) and Rape (Sunmang) are oil-seeds containing high oleic acid, and these are used for the production materials of bio-diesel and applying for phytoremediation. The effect of cadmium on germination rate and the growth of the plants is evaluated. Object seeds were placed in Cd (0, 1.5, 4, 12, 30, 60, 100, 150, 300, 500 mg/L) solutions for seven days, and germination rate, root length, shoot length, seedling length, and dry weight were observed. $IC_{50}$, seedling vigor index, and tolerance indices were computed, and data were statistically analyzed by Analysis of Variance (ANOVA). Germination rate as well as root, shoot, and seedling length decreased as the cadmium concentration increased except dry weight. The $IC_{50}$ of sunflower and rape are 112 and 10 mg-Cd/L, respectively. Only one of the sunflower seeds is germinated at 500 mg-Cd/L whereas rape seeds are not germinated more than 150 mg-Cd/L solution. Root has higher cadmium sensitivity than shoot, and sunflower has higher germination rate, growth, and seedling vigor index than rape. In case of tolerance indices, sunflower has lower value than rape at relatively low concentration, but has higher value at high concentration.

• Korean Journal of Plant Resources
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• v.15 no.3
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• pp.237-242
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• 2002

Loaves, stems, cotyledons, and roots of Hovenia dulcis Thunb grown in test tube were cultured on media containing different concentrations of single or combined growth regulators. In MS media containing 2mg/ι BA, the shoot formation rate was 95.5% and it was the highest frequency of shoot formation. MS media showed most efficiency in the shoot formation at 0.01mg/ι TDZ for the callus formation, but the color of callus changed to brown at a higher concentration of TDZ. Callus formation was 89.% at 0.5mg/ 2.4-D, but IAA, IBA, and NAA were not effective on the formation of callus. Calli were formed only on wound area when IAA, IBA, and NAA were added into MS media. Combined growth regulators (BA + auxin) were more effective in roots and nodes than leaves and cotyledons on the formation of shoot. More than 97％ of shoot formation was obtained on MS media containing BA and auxin. For the production of multiple shoot, nodes of Hovenia dulcis were used and effect of growth regulators on the formation of multiple shoot was evaluated on MS media. Highest shoots (5.3) of Hovenia dulcis were induced on MS media supplied with 0.1mg/ι BA and 0.1mg/ι NAA, and an average of 6.4 shoots per explant were obtained in 1/2 MS media containing same concentration and growth regulators. An average of 7 shoots per explant after 4 weeks of culture from nodes of Hovenia dulcis was produced on a woody plant medium(WPM) containing 0.1mg/ι BA and 0.1mg/ι NAA. Shoot length was 6.0 cm in average.

• Korean Journal of Medicinal Crop Science
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• v.14 no.2
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• pp.92-95
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• 2006

A method for plant regeneration via organogenesis from Pelagonium inquinans leaf disc has been developed. Mature leaf explants were collected from field-grown plants and used for the induction of adventitious shoot regeneration on Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose plus plant growth regulators. Maximum shoot organogenesis, with $11.8{\pm}1.5$ shoots (98.6%) per leaf disc, was obtained with $2\;mg/l$ $N^6-benzyladenine$ (BA) and $0.5\;mg/l$ ${\alpha}-naphthyleneacetic$ acid (NAA) in 30 days. For rooting, the in vitro proliferated and elongated shoots were excised into 1.5-2 cm in length microcutting, which were plated individually on an half-strength MS (1/2MS) medium supplemented with 2% (w/v) sucrose plus various concentrations of indole-3-butyric acid (IBA). Shoots rooted with a frequency of 100% following culture on 1/2MS medium containing $0.5\;mg/l$ IBA.

• Plant Resources
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• v.8 no.2
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• pp.103-109
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• 2005

Lettuce (Lactuca sativa L.) is known to contain water-soluble substances that improve antioxidant status due to the richness in antioxidants. Greenhouse experiment was carried out under different shading conditions during spring lettuce growing season. Shade significantly reduced shoot weight, number of leaves and chlorophyll content, while it increased shoot length of lettuce plants. The antioxidant potential of the individual fraction was in order of n-butanol > ethyl acetate > water > n-hexane fraction, although was less than that of commonly used antioxidants, BHT and ascorbic acid. Fractions from lettuce plants dose-dependently increased DPPH free radical scavenging activity, in vitro test. By means of HPLC analysis, BuOH fraction of cultivar 'Hwahyang' (57.93 mg $100g^{-1}$) had the highest amount of antioxidant chi orogenic acid. Shading treatment increased average amount of chlorogenic acid of all cultivars in BuoH, EtOAc, hexane and water fractions by 33, 120, 144, and $58\%$, respectively. These results suggest that lettuce plants had potent antioxidant activity, and their activities were differently exhibited depending on cultivar and fraction.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.1-6
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• 2007

Effect of BA concentrations and culture methods on in vitro plant multiplication from shoot-tip cultures of Wasabia japonica was studied. Shoot-tips with leaf primordia and apical meristem were cultured on MS basal medium for all the experiments. Liquid medium for 2 weeks followed by semi-solid medium for 4 weeks containing 1.0 mg/L BA was the best to number of shoots (22.8) and shoot length (3.5 cm). Shoots proliferated could be divided into ca. 5 to 11 of cultures for the multiplication of plantlets. Divided plantlets showed root formation (90%) well onto MS basal medium without growth regulators like IBA and NAA. After rooting, all the plantlets transferred into the pots containing composed soil (bio-media Co., peatmoss $8{\sim}10%$, coir dust $66{\sim}70%$, zeolite $13{\sim}17%$, vermiculite $3{\sim}7%$, perlite $2{\sim}4%$) and grown well into whole plants with multiple shoots.

• Journal of Plant Biotechnology
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• v.36 no.1
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• pp.1-6
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• 2009

A study was conducted to investigate in vitro micro propagation of four potato cultivars of Daese, Jasim, Chubaek and Haryeng in MS medium and PM medium (a medium containing half concentration of $NH_4NO_3$ and $KNO_3$, two fold concentration of $CaCl_2$ as compared to MS medium). During $1{\sim}2$ weeks of culture, Daese and Jasim showed better shoot elongation on the solid MS medium than Chubaek and Haryeng whereas Chubaek and Haryeng did better shoot elongation on the solid PM medium. But no difference was observed after 4 weeks of culture. As compared to shoot elongation on the solid medium, it was delayed at early stage of culture in the liquid medium without shaking. Shoot formation ratio of potato (above 4 cm of shoot length) began to increase significantly after 1 week of culture and kept on increasing until 4 weeks. The four cultivars showed the different patterns of shoot growth in bioreactor. The PM medium with a quarter salt strength was effective for the regeneration of axillary buds as well as shoot elongation of Jasim and Chubaek. Daese showed vigorous regeneration of axillary buds in the PM medium with a half salt strength. On the other hand, Haryeng showed slower growth than the other three cultivars.

• Korean Journal of Weed Science
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• v.9 no.3
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• pp.177-182
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• 1989

The plant height, shoot numbers and dry matter weight in both bulrush and rice were increased by high level of nitrogen fertilizer and especially the dry matter weights in both species were sharply increased by high level of nitrogen fertilizer. The plant height, dry matter weight in rice, and shoot, and dry matter weight in bulrush were increased by high level of phosphorus fertilizer. The deficient symptom disappeared with change of low level into high level phosphorus in rice. The plant height, dry matter weight and shoot numbers in rice and bulrush were increased by high level of potassium fertilizer. In the inter-specific competition in bulrush according to various water levels, the plant heights in both species elongated longer with deep water levels, and the plant height and dry matter weight in bulrush were more sharply decreased than in rice by deep water levels. The shoot numbers and dry matter weight were critically dropped above 6-8 cm water level. In case of the shoot numbers for light source, in both species were more decreased in mixed culture than each monoculture. The exserted shoot length and weight in bulrush were larger than those in rice. The shaded shoot dry weight was more decreased in mixed culture than in each monoculture, and the decrement was larger in bulrush than in rice.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.308-313
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• 1995

In relation to shortening generation by using embryo culture of barley immature seed, it is important to find out profitable embryo age for embryo culture and to understand the relationship among embryo and other characters. Two varieties(Olbori and Dusan #12) were cultivated under two growth conditions(15/10 and 25/15$^{\circ}C$). The embryos were aseptically excised when immature seeds were collected 9, 13, 17, 21, 25 and 29 days after heading and cultured on $B_5$ medium. On the 21st day after heading, the length of embryos from top or middle part of spike was longer than that from bottom part. Embryos from bottom part under low temperature condition had the shortest length. Shoot length, root length and root number after embryo culture were little difference among three parts of spike under high temperature condition. Under low temperature, seedlings from bottom part of spike were inferior to those from top or middle part. Length of 29-day-old embryos under low temperature condition was similar to that of 17-day-old ones under high. Under high temperature condition, the length of 17-day-old embryo had positive correlation with kernel width, shoot length, root length and root number, but that of 21-day-old one didn't have. Seventeen-day-old embryos obtained from 25/15$^{\circ}C$ growth condition seem to be efficient to shortening generation by using embryo culture.