• Korean Journal of Environmental Agriculture
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• v.16 no.2
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• pp.130-135
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• 1997

Tomatoes are flooded differently 0, 5, 10 and 15cm, according to the developing stages such as flowering stage under the condition of greenhouse. Along with this, they are treated according to the time condition such as 6, 12, 24, 48 and 120 hours. The results obtained are summarized as follows. Plant height decreased in the depth of $0{\sim}10cm$ for over 48 hours, in the depth of 15cm for over 24 hours. Number of leaves was the same as in control, and it decreased over. Number of flowers and fruit setting of individuals decreased conspicuously according as the depth and the hours got greater and longer. Adventitious root occurred remarkably in the depth of $0{\sim}10cm$, for over 24 hours and in the depth of 15cm, 12 hours. Epinastic curvature increased greatly as the depth and the hours got greater and longer. Diffusion resistance of stomata cell increased as the depth and the hours got greater and longer. Diseases occurred conspicuously as the hours of flooding got longer rather than as the depth greater. The preventing of diseases caused by insecticide was observed, but it was not greater than in the seedling and transplanting stage. Fertilization was effective in the case of increasing the weight of shoot. Number of fruits per plant did not decrease in the depth of 0cm up to 24 hours, but decreased on the deeper level of flooding and increased as the hours got longer. Moreover with the exception of 120 hours per respective depth of the treatment, average weight of a fruit got greater as the depth and the hours got greater and longer. In the case of epinastic curvature and diffusion resistance, there was negative correlation between all the other investigated characters and positive correlation between weight of a fruits and average weight of a fruit.

• Korean Journal of Plant Resources
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• v.22 no.4
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• pp.349-358
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• 2009

The potential use of 4 plant species, Astragalus membranaceus var. membranaceus, Senna occidentalis, Dianthus longicalyx and Plantago asiatica, as new sprout vegetables with high antioxidant function was examined in the present experiments. Seeds of above plants were allowed to germinate under light condition, and seedlings were maintained under dark condition for shoot growth in length for contain period of time. Then the seedlings were put under light for photosynthesis (greening treatment) for the period of 0${\sim}$3 days. Samples were collected to analyze the changes in antioxidant levels and activity, and it was observed that antioxidant substances were affected by greening treatments, depending on plant species. In A. membranaceus, the contents of total polyphenol was highest with no greening, total flavonoids with 3 days greening, DPPH radical scavenging effects with no greening, ABTS scavenging with 1 day greening, $Fe^{2+}$ chelating effects with no greening, and inhibitory activity against linoleic acid peroxidation with 3 day greening. In S. occidentalis, highest levels of antioxidant activity and radical scavenging effects were obtained by 2 day greening, $Fe^{2+}$ chelating effects by no greening and inhibitory activity against linoleic acid peroxidation by 1 day greening. In D. longicalyx, highest levels of antioxidant activity and $Fe^{2+}$ chelating effects were obtained by 2 day greening, $Fe^{2+}$ chelating effects by no greening and inhibitory activity against linoleic acid peroxidation by 1 day greening. In D. longicalyx, highest levels antioxidant activity and $Fe^{2+}$ chelating effects were observed with 3 day greening, and highest radical scavenging effects and inhibitory activity against linoleic acid peroxidation with no greening treatment. In P. asiatica, antioxidant activity and radicals scavenging effects were highest with 2 day greening, whereas highest chelating effects was obtained with no greening and highset inhibitory activity against linoleic acid peroxidation with 3 day greening. As the length of greening treatments influenced the antioxidant levels and function in plant species tested in this experiments, different culture methods are recommended for different plant species to get maximum health benefits out of sprout vegetables.

• Korean Journal of Organic Agriculture
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• v.16 no.1
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• pp.127-142
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• 2008

This study was carried out to germination characteristics by temperature conditions and allelopathic effects of aqueous extracts on Amaranthus spinosus L. in order to investigate the competitive dominant in plant ecosystem and possibility application of natural herbicide. A. spinosus L. sprouted after 24 hour from seeding in $30{\sim}45^{\circ}C$ treatment. The germination ratio of A. spinosus L. was the highest estimated at 63.3(${\pm}2.9$)% in $40^{\circ}C$ treatment, followed by 33.3(${\pm}2.9$)% in $35^{\circ}C$, 1.7(${\pm}2.9$)% in $30^{\circ}C$ and 5.0(${\pm}0.0$)% in $45^{\circ}C$ treatment. It was generally decreased the Relative Germination Ratio(RGR), the Relative Elongation Ratio(RER), the Relative Fresh weight Ratio(RFR) of receptor plants by the aqueous extracts of concentration of A. spinosus L. but it was differently inhibited the kind of receptor plant species. And it was different effected among growing regions that aqueous extracts of concentration of A. spinosus L. inhibited more root than shoot. The total phenolic compounds content of A. spinosus L. was decreased gradually middle, first and last of growth stage, and gradually decreased to leaves, roots and stems. We think that A. spinosus L. appeared high germination ratio at a short period and showed allelopathic effects on other plants. Therefore A. spinosus. L. hold the competitive dominant of plant ecosystem in Jeju Island and possibility application of natural herbicide.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.4
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• pp.265-272
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• 2001

A manure compost has been identified as an alternative to fertilizer to increase soil fertility and crop production in farming fields. The aim of the present study was to evaluate the effects of pig manure compost on soil properties and rice productivity as well as to determine the optimum application rate. In 1997, a field experiment was carried out to evaluate the growth of rice on sandy loam, loam, and clay loam soils amended with 0, 5, 10, and $20Mg\;ha^{-1}$ of pig manure compost plus NPK, which decided by soil testing. Rice yields were higher in soils receiving manure compost amendment. The maxim um yields were evaluated with $7,520kg\;ha^{-1}$ in the levels of $4.2Mg\;ha^{-1}$ of pig manure compost application plus NPK in sandy loam, $7,320kg\;ha^{-1}$ in the levels of $10.7Mg\;ha^{-1}$ in loam, and $6,320kg\;ha^{-1}$ in $17.2Mg\;ha^{-1}$ in clay loam soil. The optimum application rate of pig manure compost, which decided for 95% of maximum yields, was $4.0Mg\;ha^{-1}$ in sandy loam and $7.0Mg\;ha^{-1}$ in loam and clay loam soils under the condition of chemical fertilization by soil diagnosis. An increase in rice yield indicated a better nutrient status in compost-amended soil which was supported by the higher nutrient contents of N, P and K in shoot of plants grown in soil with manure compost amendment. Addition of manure compost increased available phosphate, silicate and exchangeable K in the amended soils according to the rate of compost application rate. It can be concluded that the manure compost could be a suitable organic fertilizer for improving rice productivity and soil fertility, and an application rate of $4.0Mg\;ha^{-1}$ in sandy loam and $7.0Mg\;ha^{-1}$ in loam and clay loam soils would give the optimum rice yields in the standard fertilization by chemical fertilization.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.3
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• pp.257-263
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• 1992

Immature embryos were tested for investigating the effect of cold treatment duration and GA$_3$ concentration on the vernalization response in barley. Immature embryos were cultured on B$_{5}$ medium with GA$_3$ for 5 days, and were transplanted under 20/15$^{\circ}C$ temperature after cold treatment. Germination rate and shoot length were increased more in 20-day-old embryos than those in 13-day-old ones but germination day was decreased. Addition of 1 or 10 ppm of GA$_3$ to B5 medium was effective on the growth of immature embryos. The higher GA$_3$ concentration was, the shorter time from embryo culture to flag leaf emergence and heading was. The earlist flag leaf emergence and heading were showed on treatment of 1 or 10 ppm of GA$_3$, but GA$_3$ did not completely replace vernalization. The days to flag leaf emergence of immature embryo-cultured barley with cold treatment for two and three weeks was shortened by 3 and 18 days at 1 ppm GA$_3$, and 16 and 20 days at 10 ppm GA$_3$, respectively, when compared to 0 ppm GA$_3$ culture with cold treatment for three weeks. It was at 10 ppm GA$_3$ with 3 weeks cold treatment that showed the lowest culm length, spike length and number of grain per spike. GA$_3$ concentration was not correlated significantly with each character in 1 week cold treatment, but was significant with each character in 2 weeks. In 3 weeks cold treatment, it was except for days to heading. Correlation between cold treatment duration and culm length was negative in 0 ppm GA$_3$. In 1 or 10 ppm of GA$_3$, all characters had highly negative correlation with cold treatment duration.n.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.5
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• pp.500-506
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• 2008

Enhanced phytoremediation with EDTA or PSM(Phosphate solubilizing microorganism) was studied using green foxtail (Setaria viridis) in columns packed with 1,200 mgPb/kg contaminated soil to investigate the effects of EDTA or PSM on the plant uptake and vertical migration of Pb. EDTA, equimolar amount of total Pb in the column soil, was administered in two methods: the one was treated with 1/6 aliquots of the equimolar EDTA every week for 6 weeks and the other was treated with single dose of the equimolar EDTA before 14 days of harvest. The results showed that higher concentrations of Pb accumulated in the biomass of green fowtail after the chemical or biological treatment. The plant-root Pb concentration in PSM treatment(M), EDTA aliquot treatment(ES), and single dose treatment(E) was 2.6, 3.0, and 3.3 times higher, respectively, than that in the plant-root of control(164.7 mg/kg). The plant-stem Pb concentration in the M, ES and E treatment was 27, 37, and 40 times higher than that in the stem of control(8.1 mg/kg). The translocation factor, the ratio of shoot/root Pb concentration, was 0.6 in the two EDTA treatment, 0.5 in the M treatment, and 0.05 in the control, respectively. The largest amount of Pb was phyto-extracted in the E treatment whereas vertical migration of EDTA was significant in the ES treatment. This result showed that a single large dose of EDTA before harvest serves better for enhanced phytoremediation of Pb. Although, treatment with PSM showed less Pb phytoextraction by the plant but enhanced both the growth of plants in the column and microbial dehydrogenase activity in the soils. Therefore, enhanced phytoextraction of Pb with PSM treatment can be an alternative option for EDTA treatment, which is toxic to plants and soil ecosystem.

• Journal of Bio-Environment Control
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• v.25 no.4
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• pp.343-350
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• 2016

The aim of this study was to evaluate effects of immature compost on the amount of nutrient content, heavy metal concentration, and application rate that were used for lettuce cultivation. The characteristics of the two composts (Compost A (CA) was immature compost and Compost B (CB) was mature compost) were evaluated upon mixing with commercial soil at 0%, 25%, 50%, and 75% (w/w). The poor chemical characteristics were appeared by use of immature compost as soil amendment; the 50% and 75% rates were weakly acidic at pH 5.39 and 5.50, respectively. The total carbon content at using of 75% of the immature compost and mature compost increased the most to 14.5 and 6.5% and it significantly increased concentrations of the total nitrogen and phosphorus compared to control. As for 75% mature compost rate increased significantly the concentrations of Cu ($128mg\;kg^{-1}$), Zn ($260mg\;kg^{-1}$), Pb ($0.32mg\;kg^{-1}$) and, Cd ($0.48mg\;kg^{-1}$) compared to control, and the highest As concentration increased significantly at 75% and 50% (6.69 and $6.28mg\;kg^{-1}$) including in 25% immature compost as $6.48mg\;kg^{-1}$. However, all of the high compost rates significantly decreased the shoot biomass of lettuce. The immature compost was potentially amended at an application rate of 25% due to a slight salinity and low risk to heavy metal uptake on lettuce growth. This use may be available if the rate is lower than that used in this trial.

• Journal of Plant Biotechnology
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• v.41 no.3
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• pp.116-122
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• 2014

Total of fifty accessions of Brassica rapa with various morphological characteristics were used for production of double haploid plants though microspore culture in Brassica rapa. Among them, only 30 accessions induced embryos from microspores. The highest efficiency of embryo induction of 1.194 per bud was obtained from IT135449 of turnip type, while 3 accessions of sarson (winter oil) type did not generate embryo. The effect of heat shock periods for embryogenesis was also investigated with 4 accessions (IT135449; Turnip type, IT199710; Chinese cabbage type, IT212886; Pak choi type, IT218043; Summer oil type). The high productions of embryos were observed in IT135449, IT199710 and IT212886 when microspores were pre-cultured to $32^{\circ}C$ for 2 days. In IT218043, high embryogenesis was observed at the 3 days of heat shock treatment. The optimal condition of shoot regeneration for IT199710 was observed in MS medium supplemented with NAA $0.5mg{\cdot}L^{-1}$ and BAP $1mg{\cdot}L^{-1}$. In contrast, the IT135449 and IT212886 were observed high regeneration frequency in MS medium without plant growth regulators. All the plantlets regenerated from microspore-derived embryos have been successfully transplanted to soil, and bud self-pollinated seeds were produced from doubled haploid plants. This indicated that double-haploid genotype was likely generated naturally during embryogenesis process.

• Journal of Korean Society of Forest Science
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• v.104 no.3
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• pp.390-396
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• 2015

This study was carried out in order to closely examine quality index by shading level of 2-year-old (1-1 seedling) container seedling of Daphniphyllum macropodum which is known as the species of having shade tolerance that is evergreen broad leaved tree in the warm temperate region. The shading treatment was regulated with the shading level of full sunlight, and 35%, 55%, 75%, 95% of full sunlight. As a result of surveying growth according to the shading level, both height and root collar diameter were surveyed to be the highest with 45.1 cm and 8.22 mm, respectively, under 75% of shading. The next was surveyed to be 43.2 cm & 8.05 mm and 42.5 cm & 7.98 mm, respectively, in order of 35% and 55% in shading. Leaf, shoot, root, and whole dry mass production were the highest under 75% of shading. The next was higher in leaf, stem, and whole dry mass production under 55% of shading. A root was higher under 35% of shading in the next. H/D ratio was the range of 5.29~5.35 under the 35~75% shading that showed the relatively high height and root collar diameter. T/R ratio was the lowest with 1.17 under 35% of shading. It was 0.41 under 95% of shading as for LWR, 0.24 under 75%-95% of shading as for SWR, and 0.46 under full sun and 35% shading as for RWR. QI was the highest with 3.74 under 75% of shading. As a result of surveying the whole experiment, it is concluded that the production of D. macropodum seedling is more effective under 75% shading

• Journal of Korean Society of Forest Science
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• v.83 no.3
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• pp.311-321
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• 1994

Experiments were conducted to evaluate the efficacy of ground and foliar sprinkling of natural water for control of the pine needle gall midge, Thecodiplosis japonensis, by disturbing the reproductive behavior of adults such as copulation and oviposition. Diurnal ground spray for whole area application and foliar spray for single tree treatment were tested at day time during the period of adult emergence. The results obtained are as follows : 1. The population density of overwintering larvae in soil was not affected by the water spray during the adult emergence period. 2. Spraying the water showed no effect on the whole period and number of adult emergence, but retarded early emergence and facilitated Late emergence. The difference in the emerging time by 50% emergence day was approximately 7 days. 3. Ground spray was significantly different from control in gall formation rates but was not sufficient for effective control. As a single-tree treatment, the foliar spray was found to be highly effective for control of the pine needle gall midge, Average gall formation of spray plots and untreated ones were 2.6% and 38%, respectively ; control value exceeded 90%. 4. Ground spray of water was not effective in reducing larvae in gall of needle. Foliar spray, however, reduced 45% of larval numbers. 5. Foliar spray accelerated shoot growth by 15%-18%. 6. Foliar spray was highly effective for the control of sucking insects, such as mites and aphids on pine trees.