• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1991.04a
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• pp.127-132
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• 1991

충격에 민감한 용기나 기계들은 운반이나 보관중에 받을 수 있는 진동충격 을 완화시키기 위해 충격 마운트를 이용하게 되며, 충격마운트는 용기나 기 계들이 허용하는 최대가속도와 이들이 취급되는 환경에 의해 적절히 선정되 어야 한다. 그러므로 내충격 설계는 최대 허용 가속도나 취급환경에 대한 사 양과 이 사양에 의한 충격 마운트의 선정, 그리고 선정된 내충격 시스템의 충격시험에 의한 오일 탬퍼의 결합용이 사용되기도 하지만 구조가 복잡하고 가격이 비싸서 대용량이 경우에만 국한되어 사용되고 있으며, 가장 경제적인 것은 방진고무를 사용한 것이다. 내충격 시스템은 일반적으로 큰 변형량을 요구하고 있으며, 방진고무의 경우 큰 변형에서는 hardening 스프링의 비선 형성을 가지고, 형상계수와 고무배합율에 따라 정적 스프링상수와 동적 스프 링상수가 큰 차이를 보이는 등, 설계상의 여러 제한이 있다. 그러나 방진고 무는 형상변화가 자유로우며 제작이 용이하기 때문에 이러한 장점을 이용하 면 저렴하고 다양한 용도의 충격마운트 제작에 효과적으로 사용될 수 있다. 본 논문에서는 원통형 콘테이너의 내충격 설계를 위해 충격사양에 의한 충 격마운트의 설계, 설계된 내충격 시스템의 충격시험에 의한 검증을 순차적으 로 실시하여, 방진고무를 이용한 충격마운트 설계기술을 제시함으로써, 이후 방진고무제품의 설계를 위한 자료 및 과정의 제정에 도움을 주고자 한다.

• Journal of Pharmacopuncture
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• v.14 no.4
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• pp.23-32
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• 2011

Objectives: This study was performed to examine the antigenic potential of pure melittin (Sweet Bee Venom - SBV) extracted from the bee venom by utilizing protein isolation method of gel filtration. Methods: All experiments were conducted at Biotoxtech (Chungwon, Korea), authorized a non-clinical studies institution, under the regulations of Good Laboratory Practice (GLP). Antigenic potential of SBV was examined by active systemic anaphylaxis (ASA) and passive cutaneous anaphylaxis (PCA) in guinea pigs. SBV was subcutaneously administered at 0.07 and 0.28mg/kg and also as a suspension with adjuvant (Freund's complete adjuvant: FCA). Ovalbumin (OVA) as a suspension with adjuvant was used to induce positive control response ($5mg/m{\ell}$-FCA). Results: 1. In the ASA test, experimental groups showed some symptoms of anaphylaxis like piloerection, hyperpnea and staggering gait. 2. In the PCA test, low dosage group did not show any antibody responses, whereas high dosage group showed positive responses. 3. In the weight measurement and clinical observation, experimental groups didn't show any significant changes compared with control group. 4. In the autopsy of body, the abnormalities of lung were detected in the corpse. This means that the cause of death may induced anaphylactic shock. Conclusions: Above findings suggested that SBV had antigenic potential in guinea pig. Further studies on the subject should be conducted to yield more concrete evidences.

• Microbiology and Biotechnology Letters
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• v.30 no.4
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• pp.320-324
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• 2002

To establish a rapidly immunochemical identification on a dinoflagellate, Cochlodinium polykrikoides, a specific antigenic protein as a maker on the cell membrane was isolated. The cell membranes of C. polykrikoides and Gymnodinium sangineum were harvested by centrifugation after osmotic shock. The membrane proteins of both cells were solubilized in 50 mM Na-carbonate contained 1 mM DTT, and separated the proteins on SDS-PACE. Immune-blot on the solubilized membrane proteins of the both cells was performed with antiserum against the solubilized membrane proteins of C. polykrikoides. A 120 kDa membrane protein of C. polykrikoides had remarkablely different antigenicity from that of G. sangineum.

• Journal of Power System Engineering
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• v.19 no.3
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• pp.69-74
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• 2015

Check valves used in vessels include shock-release function on piping system, aside from basic back flow prevention. However, proper and enough protection of system is not obtainable due to use of high-pressure and bulk fluids, resulting from enlargement of vessels. In this study, casting analysis of check valves protecting systems in flow path from water hammering or back flow is conducted, using Z-CAST program. Also, molten metal filling, flow analysis, solidification analysis and shrinkage cavity analysis are conducted. The main results are as following. Regarding filling of each risering, molten metal showed stable supply condition without being isolated. It was identified that the final solidification exists on risering, but shrinkage cavity possibly might happens at the point of isolation solidification.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.3 no.1
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• pp.98-104
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• 2000

Pseudomembranous colitis, thought to be uncommon in children, is a bacterial, toxin-mediated inflammatory process resulting in acute or chronic diarrhea and is characterized by colonic pseudomembranes. It is mediated by toxins produced by Clostridium difficile and is increasingly recognized in pediatric population. Diagnosis is based on positive culture of C. difficile in selective media and positive test of C. difficile toxin. Oral metronidazole or vancomycin are the main treatment options but avoidance of further antibiotics should also be encouraged where possible. We have experienced a case of pseudomembranous colitis in a 4-year-old female presented with septic shock and colitis. This case was diagnosed with positive test of C. difficile toxin B and confirmed by isolation of the organism on cultire in selective media. Symptoms have been ameliorated by discontinuation of antibiotics and administration of metronidazole and oral vancomycin, and ICU care.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.13 no.2
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• pp.132-143
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• 2003

Viscoelastic components for vibration isolation or shock absorption in automobiles, machines and buildings are often subject to a high level of static deformation. From the dynamic design point of view, it is requisite to predict dynamic complex stiffness of viscoelastic components accurately and efficiently. To this end, a systematic procedure for complex modulus measurement of the viscoelastic material under large static deformation is often required in the industrial fields. In this paper, dynamic test conditions and procedures for the viscoelastic material under small oscillatory load superimposed on large static deformation are discussed. Various standard test methods are investigated in order to select an adequate test methodology. The influence of fixed boundary condition in the compression tests upon complex stiffness are investigated and an effective correction technique is proposed. Then the uniaxial tension and compression tests are performed and its results are compared with analysis results from conventional constitutive models.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2004.11a
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• pp.565-569
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• 2004

Conventional methods for reducing vibration in engineering designs (i.e. by stiffening or detuning) may be undesirable or inadequate in conditions where size or weight must be minimized or where complex vibration spectra exist. Alloys which combine high damping capacity with good mechanical properties can provide attractive technical and economic solutions to problems involving seismic, shock and vibration isolation. To meet these trends, we have developed a new high damping Fe-17%Mn alloys. Also, the alloy has advantages of good mechanical properties and more economical than any other known damping alloys(1/4 times as cost of non-ferrous damping alloy). Thus, the high damping Fe-17%6Mn alloy can be widely applied to household appliances, automobiles, industrial facilities and power plant components with its excellent damping capacity(SDC, 30%) and mechanical property(T.S 700MPa). It is the purpose of this paper to introduce the characterization of the high damping Fe-17%Mn alloy and the results of retrofit several such applications.

• Korean Journal of Microbiology
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• v.52 no.4
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• pp.444-454
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• 2016

Sixteen lactic acid bacterial strains were isolated from silage and cow dung samples, and characterized to identify their potential as silage additives. They were identified as the members of the genera Lactobacillus, Enterococcus, and Weissella, and clustered into nine groups based on the sequences of the genes for 16S rRNA, RNA polymerase alpha subunit, 60-kDa heat shock protein, and phenylalanyl-tRNA synthase alpha subunit. Among them, the three strains which were genetically similar to L. plantarum showed the fastest growth and pH decrease in MRS and rye extract media, the highest numbers of available carbohydrates, and the widest ranges of pH, temperature, and salinity for growth. In addition, they showed no amplified DNA products in the PCR examination targeting the genes for the production of biogenic amines, and the MRS media where they had been cultured showed relatively high inhibition effect against the growth of silage-spoiling microorganisms, including fungi, yeast, and clostridia. The results suggest that these strains are good candidates for silage additives. However, the rye extract media where the lactic acid bacteria had been cultured had no effect on or stimulated the growth of the silage-spoiling microorganisms, and the causes must be established for the practical use of the lactic acid bacteria as silage additives.

• Tuberculosis and Respiratory Diseases
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• v.69 no.6
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• pp.469-473
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• 2010

Nocardia farcinia, an aerobic, gram-positive bacilli actinomycetes of the genus Nocardia, is an uncommon pathogen found in humans. The most common Nocardia infection sites are the lung, central nervous system, and skin. Even though hematogenous dissemination can occur, isolation of the organism from blood cultures is very rare. We report a case of Nocardia infection that was isolated on blood cultures. A 59-year-old male with a medical history that includes a liver transplantation 6-years prior due to hepatocellular carcinoma secondary to chronic hepatitis B, developed pneumonia and was transferred to Severance Hospital. At the time of admission, the patient's initial exam showed hypothermia, tachypnea, and hypotension. His chest radiograph showed severe pneumonia and a large abscess on left upper lobe. Under the presumptive diagnosis of bacterial pneumonia or other opportunistic infection, we started broad spectrum antibiotics. However, he developed Nocardia sepsis, rapidly deteriorated, and subsequently died.

• BMB Reports
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• v.45 no.6
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• pp.331-336
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• 2012

The retinoid-related orphan nuclear receptor gamma ($ROR{\gamma}$) plays critical roles in regulation of development, immunity and metabolism. As transcription factor usually forms a protein complex to function, thus capturing and dissecting of the $ROR{\gamma}$ protein complex will be helpful for exploring the mechanisms underlying those functions. After construction of the recombinant tandem affinity purification (TAP) plasmid, pMSCVpuro $ROR{\gamma}$-CTAP(SG), the nuclear localization of $ROR{\gamma}$-CTAP(SG) fusion protein was verified. Following isolation of $ROR{\gamma}$ protein complex by TAP strategy, seven candidate interacting proteins were identified. Finally, the heat shock protein 90 (HSP90) and receptor-interacting protein 140 (RIP140) were confirmed to interplay with $ROR{\gamma}$ by co-immunoprecipitation. Interference of HSP90 or/and RIP140 genes resulted in dramatically decreased expression of CYP2C8 gene, the $ROR{\gamma}$ target gene. Data from this study demonstrate that HSP90 and RIP140 proteins interact with $ROR{\gamma}$ protein in a complex format and function as co-activators in the $ROR{\gamma}$-mediated regulatory processes of HepG2 cells.