This study was carried out to develop transgenic rice cultivars with the CAX1 (accession no. U57411) gene. We successfully selected the transgenic rice plants over-expressing the Arabidopsis H+/$Ca^{2+}$ antiporter CAX1 (accession no. U57411) gene in T6 generation. The brown rice of the CAX1 expressing rice contained 13.4~68.0 % more calcium $(Ca^{2+})$ than that of the wild type and 5 lines were selected based on the phenotypes compared to the control cultivar at the GMO field. The CAX1 expressing transgenic rice plants were similar in phenotype to the wild type during the whole growth period. Also these selected 4 lines appeared to be resistant to blast, cold and water solution compared with the wild type. Difference in 1,000 grain weight of brown rice was observed among each line but grain shape did not show any morphological alternations. These results suggest the enhanced Ca-substrate specificity of CAX1 exchanger in donor plant. Therefore, intact CAX1 exchanger can be functionally useful for $Ca^{2+}$ nutrient enrichment of rice with reduced accumulation of undesirable cation.
Park, Ji-Hyun;Choi, Gyung-Ja;Lee, Seon-Woo;Jang, Kyoung-Soo;Choi, Yong-Ho;Chung, Young-Ryun;Cho, Kwang-Yun;Kim, Jin-Cheol
The Korean Journal of Mycology
/
v.32
no.1
/
pp.31-38
/
2004
An endophytic bacterial strain EB215 that was isolated from cucumber (Cucumis sativus) roots displayed a potent in vivo antifungal activity against Colletotrichum species. The strain was identified as Burkholderia cepacia based on its physiological and biochemical characteristics, and 16S rDNA gene sequence. Optimal medium and incubation period for the production of antifungal substances by B. cepacia EB215 were nutrient broth (NB) and 3 days, respectively. An antifungal substance was isolated from the NB cultures of B. cepacia EB215 strain by centrifugation, n-hexane partitioning, silica gel column chromatography, preparative TLC, and in vitro bioassay. Its chemical structure was determined to be pyrrolnitrin by mass and NMR spectral analyses. Pyrrolnitrin showed potent disease control efficacy of more than 90% against pepper anthracnose (Colletotrichum coccodes), cucumber anthracnose (Colletotrichum orbiculare), rice blast (Magnaporthe grisea) and rice sheath blight (Corticium sasaki) even at a low concentration of $11.1\;{\mu}g/ml$. In addition, it effectively controlled the development of tomato gray mold (Botrytis cinerea) and wheat leaf rust (Puccinia recondita) at concentrations over $33.3\;{\mu}g/ml$. However, it had no antifungal activity against Phytophthora infestans on tomato plants. Further studies on the development of microbial fungicide using B. cepacia EB215 are in progress.
This study was to find out optimum rice cultivars for organic farming in no-tillage paddy. A field research was conducted the yield, yield components, and qualities of thirty two Korean rice cultivars, and two Japanese rice cultivars. The column length of early rice plant was shorter in medium maturing rice cultivars compared to early, and medium-late maturing rice cultivars. Planthopper population per20-plant was lower in Ilpumbyeo, Dongjinbyeo, Hojinbyeo, Donganbyeo, and Sobeebyeo in that order while was higherin rice cultivars of early maturity. The disease severity of sheath blight was higher in Saechucheongbyeo>Chucheongbyeo>Namwonbyeo>Sangmibyeo in that order, on the other hand, that was lowerinHwaseongbyeo>Junambyeo>Saesangjubyeo=Hitomebore>Ilpumbyeo>Hwayeongbyeo in that order, respectively. The grain yield of Korean rice cultivars was significantly higher in 2.95 Mg $ha^{-1}$ for Hwasinbyeo, 2.91 Mg $ha^{-1}$ for Ilpumbyeo, 2.86 Mg $ha^{-1}$ for Sobeebyeo, 2.73 Mg $ha^{-1}$ for Naepungbyeo, and 2.70 Mg $ha^{-1}$ for Saegyehwabyeo compared with 1.38 Mg $ha^{-1}$ for Hwabongbyeo, 1.62 Mg $ha^{-1}$ for Hwayeongbyeo, and 1.78 Mg $ha^{-1}$ for Haepyeongbyeo of medium maturing rice cultivars, respectively. Toyo taste value of medium maturing rice cultivars tended to higher than that of early, and medium-late maturing rice cultivars. Ripening rate of rice cultivars was significantly positive correlated with Toyo taste value while negative correlated with protein content. According to principal component analysis, these results show that Sangmibyeo, Sangsanbyeo, and Odaebyeo for early maturity, Naepungbyeo, and Sobeebyeo for medium maturity, and Hwasinbyeo, Chucheongbyeo, and Sindongjinbyeo for medium-late maturity were optimum rice cultivars for organic farming in no-tillage paddy.
The objectives of this study were to evaluate no-tillage cover crop cropping systems for organic farming in paddy. The experiment was performed at Ihyeon series (silt loam: 9.1% sand, 73.0% silt and 17.9% clay) which affected by different management practices. Planthopper population per 20 plant was significantly higher 65.3 for conventional tillage with chemical compounds (control) compared with 3.4~9.6 for no-tillage treatments (without rice straw or green manure, amended with rice straw, hairy vetch, rape, rye, and Chinese milk vetch). Also, disease severity of sheath blight was significantly higher 10.5% for control compared to 0.7~2.9% for no-tillage treatments. Four weed species, namely Monochoria vaginalis, Ludwigia prostrata, Rotala indica, and Aneilema keisak occurred in no-tillage paddy, whereas Monochoria vaginalis occurred in control only. The pH, available phosphate, and microbial biomass C in paddy were steeply decreased in response to submerging, but increased at first heading stage. Soil $NH_4$-N content at first heading stage was significantly higher in no-tillage treatments compared with control. The grain yield was significantly higher in 4.30 Mg $ha^{-1}$ for control than other treatments. Meanwhile, rice productivity was significantly higher in 2.69 Mg $ha^{-1}$ for no-tillage amended with Chinese milk vetch compared to other no-tillage treatments. The number of panicle per plant, grain number perpanicle, and percent ripened grain were highly related for increasing the yield of rice. These results show that Chinese milk vetch was optimum cover crop for organic farming in no-tillage paddy.
Journal of The Korean Society of Grassland and Forage Science
/
v.27
no.4
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pp.287-296
/
2007
The experimental work was conducted to determine the growth characteristics and yield of whole crop rice (cv. Suwon 468 and cv. Chuchungbeo) and soil properties using various type of livestock manure application on rice paddy land for 3 years ($2003{\sim}3005$). Compared Suwon 468 and Chuchungbeo, Suwon 468 has longer plant height and more DM yield than that of Chuchungbeo. Among livestock manure type, plant height was longer in order of liquid swine manure (LSM) > composted swine manure (CSM) > chemical fertilizer (CF) > composted cattle manure (CCM). Number of branch on Chuchungbeo had more than that of Suwon 468. Among livestock manure type, number of branch had more in order of LSM > CSM > CF > CCM. DM yield of whole crop rice (WCR) was affected by various types of livestock manure application and increased in order CSM > CCM = LSM. DM yield on the effects of application level of LSM was highest in LSM 75% + CF 25%. Plant diseases such as rice blast, damage by insect, smut, sheath blight occurred in LSM and CSM and there was not significantly different among application level of LSM. The nitrogen content of WCR by CSM was the highest of all treatments and the ripened ratio by CSM was in contrary order. Moreover the feed value of WCR was not significantly different among treatments. Soil pH, organic matter and total nitrogen was not different by LSM application whereas phosphorus content increased by LSM application. Cu and Zn content increased by LSM and CSM application and were not different by CCM as compared to control plots.
To investigate a comparison of rice yield and quality by a utilization of green crops using barley and hairyvetch, this study was carried out in rice (Oryza sativa L.) organic cultivation field. Convention barley harvest (CBH), Green barley crop (GBC), Green barley+Green Hairyvetch crop (GB+HVC), and Non-Green crop (NGC) were treated in rice cultivation field. The results obtained as following: 1) Culm length and pancile length in Convention barley harvest (CBH) cultivation were longer than organic cultivation but were not significant difference in all treatments. Yields of milled rice in Green barley crop (GBC) and Green barley+Green Hairyvetch crop (GB+HVC) were by 90.3% and 95.9%, respectively. 2) Protein contents in Green barley crop (GBC) and Green barley+Green Hairyvetch crop (GB+HVC) were by 5.7% lower than 6.6% in Convention barley harvest (CBH) cultivation. Amylose contents in all treatment were similar as range 18.8% to 19.1%. Whiteness contents in Green barley crop (GBC) and Green barley+Green Hairyvetch crop (GB+HVC) were slightly higher by 38.9% and 39.1%, respectively, than 37.7% in Convention barley harvest (CBH) cultivation. 3) Palatability values in Barley harvest (BH) and Non-Green crop (NGC) were slightly higher by 82.0 and 83.8, respectively, than 77.6 in Convention barley harvest (CBH) cultivation. 4) Head rice in Non-Green crop (NGC) was 95.5%, while that of Convention barley harvest (CBH), Green barley crop (GBC), and Green barley+Green Hairy-vetch crop (GB+HVC) were slightly low as range 93.8% to 94.2%. White core and belly rice in Convention barley harvest (CBH) cultivation was the highest level by 1.7%. 5) Leaf blast, neck blast, sheath blight, rice stem maggot, rice leaf roller, rice leaf-tier, and green rice leafhopper were occurred lightly in rice field.
Rhizoctonia solani [Thanatephorus cucumeris (frank) Donk], one of the major soil-borne plant pathogens with world-wide distribution, can cause great damages on various crops. In Korea, sheath blight on rice caused by this pathogen is the major concern, and active studies on this pathogen have been performed. However, most of these studies were concerned with pathogenicity of the isolates instead of molecular analyses of different AGs of R. solani. Therefore, in this study, thirty isolates of Rhizoctonia solani collected from various sources were used for the analyses of genetic relationships among themselves and for the rapid anastomosis grouping with RAPD method. As a result, thirty isolates of known and unknown AGs were grouped into five subgroups and each group included AG-1, AG-2, AG-3, AG-4, and AG-5. RS-1 isolate was found to be closely related to AG-5. Isolates RS-4, RS-14, RS-17, and RS-16 were found to be closely related to AG-2-2(III B). Isolate RS-13 was closely related to AG-4, isolates RS-8 and RS-10 were closely related to AG-1(I B), and isolates RS-7 and RS-21 were closely related to AG-2-2(IV). Isolate RS-19 was closely related to AG-1(I C), and isolates RS-3, RS-5, RS-18, RS-6, and RS-15 were found to be closely related to AG-1.
The purpose of this study was to find out optimum cultivated regions for rice organic farming. The organic and conventional rice as control were grown in three different places : secluded hill paddy field for Hapcheon, normal rural paddy field for Sancheong, and suburban paddy field for Jinju from 2005 to 2006. In secluded hill paddy field, the organic material and pesticide to control pest and disease were input twice for organic and conventional rice cultivation. However, in normal rural and suburban paddy field, those were input three times for organic and conventional rice cultivation. The occurrence of sheath blight in organic farming was higher than in conventional farming. Whereas brown planthopper population per 20 plant was significantly high 10.1~19.5 for conventional farming compared with 4.4~10.0 for organic farming. For that reason, the density of the brown planthoppers was higher in organic farming than those in conventional farming. Dominated weeds occurred in organic and conventional paddy field were namely Monochoria vaginalis, Ludwigia prostrata, and Cyperus difformis. The population per 20 plant and dried weight per $m^2$ of weeds were higher in 121 and 50.5 g for organic paddy field. The productivity of rice in different cultivated regions for organic farming was $2.96Mg\;ha^{-1}$ in hill paddy field, $4.03Mg\;ha^{-1}$ in normal rural and suburban paddy field. Toyo-taste value and ratio of perfect grain of milled rice were not different by cultivated regions in both farming system.
The locational preference of the brown planthopper (BPH) Nilaparvata lugens ($St{\aa}l$) and the whitebacked plant hopper (WBPH) Sogatella furcifera(Horvath) was studied on rice cultivars IR22 and IR36 as an integral part of subsequent research on insect-fungal pathogen relationships. The BPH was observed to stay consistently on the basal portion while the WBPH showed a general preference for the upper portion regardless of varieties, rice growth stages and insect population density levels. The habitat preference of both species (BPH and WBPH) was found not to be affected by the presence of the other species when both species are present on the same host plant Five rice cultivars with different reactions to BPH biotype 2 were used in the study on BPH-Rhizoctonia solani relationship: IR22 and TN1 (susceptible); Triveni and ASD7 (moderately resistant); and IR42 (resistant). Test plants were inoculated with R. solani (Kuhn) $3{\sim}4$days after insect infestation. Sheath blight disease severity/incidence was significantly higher in the treatment where BPH+R. solani were together than in the treatment with only the pathogen. Symptom expression of the disease in the BPH-pathogen combination was faster and mycelial growth was more profuse inducing the formation of more infection structures. Regardless of varietal reaction to BPH biotype 2, the degree of hopperburn was significantly higher in the combination of the two pests as compared with that of BPH alone. There could be a synergistic relationship between the insect pest and the pathogen indicated by a positive interaction between the two species.
Journal of Practical Agriculture & Fisheries Research
/
v.20
no.1
/
pp.5-18
/
2018
The field trial was performed to evaluate the rice growth and yield in direct seeding cultivation with iron-coated rice seeds. The required time for seed emergence was for 9~11days in the tested direct seeding methods. That was 1~2days earlier in direct seeding with pregerminated seeds than that of direct seeding with iron-coated seeds. The seedling establishment was highest in water seeding with iron-coated seeds but there was not significant difference in terms of statistical analysis. The rice plant height was taller in water seeding with broadcasting method than that of wet hill-seeding methods and in direct seeding with iron-coated seeds than that of direct seeding with pregerminated seeds. The tiller number in the rice plant was the highest in machine transplanting at 30days after direct seeding(June 17) and in water seeding with iron-coated seeds at 45days after seeding(DAS) and 60DAS. The tiller number of 75 and 90DAS in the tested rice cultivation methods being with 352~405/m2 was not significantly different in terms of statistical analysis. The heading time was not different in rice direct seeding methods but 2 day earlier in direct seeding with iron-coated seeds than that of direct seeding with pregerminated seeds. The culm length was the highest in water seeding with iron-coated seeds and the panicle length was the longest in wet hill-seeding with pregerminated seeds. The panicle number per m2 was highest in water seeding with iron-coated seeds but not significant difference among the tested rice cultivation methods. The water seeding with iron-coated seeds resulted in the highest spikelet number per m2 and the heaviest grain weight of brown rice. Percentage of ripened kernel was the highest in wet hill-seeding with iron-coated seeds. But there were not significant among the tested rice cultivation methods. The milled rice yield in direct seeding methods was 3~21% higher than that in machine transplanting. Water seeding with iron-coated seeds recorded the highest milled rice yield being with 6.86t/ha.The occurrence of sheath blight was high according to machine transplanting>wet hill-seeding>water seeding. Weed occurrence was the highest in water seeding with pregerminated seeds. Weedy rice occurred not in machine transplanting but occured 0.6~0.7% in direct seeding methods with pregerminated seeds and 0.1% in direct seeding with iron-coated seeds.
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