• Restorative Dentistry and Endodontics
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• 제14권1호
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• pp.57-70
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• 1989

The purpose of this study was to investigate the effect of salivary gland on the calcification of dentin in rats. 80 Sprague-Dawley male rats that weighed approximately 120gm were used in this study. 5 rats among them were shared as controls. 75 rats received sialoadenectomy were divided into submaxillary adenectomy group, parotidectomy group, and submaxillary-parotid gland combined removal group. In experimental groups, 25 rats in each of the 3 groups were sacrificed at the following intervals; 3 days, 1, 2, 3 and 4 weeks. All animals were sacrificed by vascular perfusion with 10% formalin. The maxillary incisors including periapical tissues were removed and defatted in 20% KOH solution at $0^{\circ}C$ for 24 hours, and dehydrated with acetone. Each tooth specimen was attached on the stab for scanning electron microscopic study. Gold was coated on the each specimen in the thickness of 300${\AA}$ at D.C. 1400V, 6mA for 6 minutes with coating machine (Eiko IB-3). Inner dentinal surfaces of the specimens were observed with SEM (Hitachi S-450). The results were as follows, 1. Parotidectomy groups were found to be inhibited the formation of dentinal calcification compared to submaxillary adenectomy groups in the eady stages. 2. Combined removal of submaxillary and parotid gland was appeared to cause more severe inhibition effect on the dentinal calcification than that of each salivary gland separately. 3. Inhibition of the calcification and mineralization of dentin caused by sialoadenectomy was more extreme from 3 day to 2 weeks after beginning of the experiments. However it was tended to be normalized after that. 4. Salivary gland was responsible for alterations in calcification and mineralization of dentinal growth.

• 한국콘텐츠학회논문지
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• 제9권3호
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• pp.175-184
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• 2009

2006년도에는 한국의 세계 문화콘텐츠 시장 점유율은 2.4%로 9위를 차지하고 있다. 따라서 문화콘텐츠 산업을 국내에서는 신성장동력산업으로 분류하고 있으며, 이에 따라 정부에서는 통합 콘텐츠정책추진체계 를 정비하면서, 불법복제를 근절, 저작권을 보호하고, 콘텐츠창작 역량을 강화하고 있으며, 기업의 해외시장개척에 지원을 하고 있다. 이렇듯 문화콘텐츠산업의 중요성은 나날이 부각되고 있다. 이에 본 연구에서는 산업연관분석을 통하여 문화콘텐츠산업의 생산유발효과, 부가가치유발효과, 취업유발효과, 공급지장효과 그리고 물가파급효과에 대한 시계열적인 흐름에 따른 크기의 변화를 분석하였다. 특히 물가파급효과의 경우 시간의 흐름에 따라 증가하였다. 이는 문화콘텐츠산업의 산출물의 가격변화가 타 산업에 미치는 영향이 점차 증가하고 있다는 것을 의미한다.

• Journal of Plant Biotechnology
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• 제36권4호
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• pp.407-414
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• 2009

A cDNA clone encoding phytocystatin was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (name as Brcpi1; GenBank accession no.: EF079953) had a total length of 881 bp with an open reading frame of 609 bp, and encoded predicted polypeptide of 203 amino acid (aa) residues including a putative N-terminal signal peptide. Other relevant regions found its sequence included the G and PW conserved aa motifs, and the consensus LARFAV sequence for phytocystatins and the reactive site QVVAG. The BrCPI1 protein shared 95, 94, 81, 80 and 78% identity with other CPI proterins isolated from Brassica oleracea (BoCPI-1), Arabidopsis thaliana (AtCY SB), Glycine max (GmCPI), Oryza sativa (OsCYS-2) and Zea may (ZmCPI) at amino acid level, respectively. Southern blot analysis showed that Brcpi1 was a low copy gene. Expression pattern analysis revealed that Brcpi1 was a tissue-specific expressing gene during reproductive growth and strongly expressed at mature seedling stages. Furthermore, overexpression of Brcpi1 in transgenic Arabidopsis was enhanced tolerance to salt and cold stresses. Meanwhile the juvenile seedling of Brcpi1 transgenic plants was not affected by various concentrations ABA in MS medium. Taken together, the results showed that Brcpi1 functioned as a cysteine protease inhibitor and it exhibited a protective agent against diverse types of abiotic stress, which induced this gene in a tissue- and stress-specific manner.

• 한국IT서비스학회지
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• 제16권3호
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• pp.167-183
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• 2017

The rapid growth of information technology and mobile service platforms, i.e., internet, google, and facebook, etc. has led the abundance of data. Due to this environment, the world is now facing a revolution in the process that data is searched, collected, stored, and shared. Abundance of data gives us several opportunities to knowledge discovery and data mining techniques. In recent years, data mining methods as a solution to discovery and extraction of available knowledge in database has been more popular in e-commerce service fields such as, in particular, movie recommendation. However, most of the classification approaches for predicting the movie popularity have used only several types of information of the movie such as actor, director, rating score, language and countries etc. In this study, we propose a classification-based support vector machine (SVM) model for predicting the movie popularity based on movie's genre data and social network data. Social network analysis (SNA) is used for improving the classification accuracy. This study builds the movies' network (one mode network) based on initial data which is a two mode network as user-to-movie network. For the proposed method we computed degree centrality, betweenness centrality, closeness centrality, and eigenvector centrality as centrality measures in movie's network. Those four centrality values and movies' genre data were used to classify the movie popularity in this study. The logistic regression, neural network, $na{\ddot{i}}ve$ Bayes classifier, and decision tree as benchmarking models for movie popularity classification were also used for comparison with the performance of our proposed model. To assess the classifier's performance accuracy this study used MovieLens data as an open database. Our empirical results indicate that our proposed model with movie's genre and centrality data has by approximately 0% higher accuracy than other classification models with only movie's genre data. The implications of our results show that our proposed model can be used for improving movie popularity classification accuracy.

• Fisheries and Aquatic Sciences
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• 제19권8호
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• pp.33.1-33.7
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• 2016

Antifreeze proteins (AFPs) lower the freezing point but not the melting point of aqueous solutions by inhibiting the growth of ice crystals via an adsorption-inhibition mechanism. However, the function of type IV AFP (AFP IV) is questionable, as its antifreeze activity is on the verge of detectable limits, its physiological concentration in adult fish blood is too low to function as a biological antifreeze, and its homologues are present even in fish from tropic oceans as well as freshwater. Therefore, we speculated that AFP IV may have gained antifreeze activity not by selective pressure but by chance. To test this hypothesis, we cloned, expressed, and assayed AFP IV from cultured subtropical olive flounder (Paralichthys olivaceus), which do not require antifreeze protein for survival. Among the identified expressed sequence tags of the flounder liver sample, a 5'-deleted complementary DNA (cDNA) sequence similar to the afp4 gene of the longhorn sculpin was identified, and its full-length cDNA and genome structure were examined. The deduced amino acid sequence of flounder AFP IV shared 55, 53, 52, and 49 % identity with those of Pleuragramma antarcticum, Myoxocephalus octodecemspinosus, Myoxocephalus scorpius, and Notothenia coriiceps, respectively. Furthermore, the genomic structure of this gene was conserved with those of other known AFP IVs. Notably, the recombinant AFP IV showed a weak but distinct thermal hysteresis of $0.07{\pm}0.01^{\circ}C$ at the concentration of 0.5 mg/mL, and ice crystals in an AFP IV solution grew star-shaped, which are very similar to those obtained from other polar AFP IVs. Taken together, our results do not support the hypothesis of evolution of AFP IV by selective pressure, suggesting that the antifreeze activity of AFP IV may have been gained by chance.

• Journal of Microbiology and Biotechnology
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• 제20권5호
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• pp.893-903
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• 2010

A cellulolytic and xylanolytic enzyme complex-producing alkalothermoanaerobacterium strain, Tepidimicrobium xylanilyticum BT14, is described. The cell was Grampositive, rod-shaped, and endospore-forming. Based on 16S rRNA gene analysis and various lines of biochemical and physiological properties, the strain BT14 is a new member of the genus Tepidimicrobium. The strain BT14 cells had the ability to bind to Avicel, xylan, and corn hull. The pH and temperature optima for growth were 9.0 and $60^{\circ}C$, respectively. The strain BT14 was able to use a variety of carbon sources. When the bacterium was grown on corn hulls under an anaerobic condition, a cellulolytic and xylanolytic enzyme complex was produced. Crude enzyme containing cellulase and xylanase of the strain BT14 was active in broad ranges of pH and temperature. The optimum conditions for cellulase and xylanase activities were pH 8.0 and 9.0 at $60^{\circ}C$, respectively. The crude enzyme had the ability to bind to Avicel and xylan. The analysis of native-PAGE and native-zymograms indicated the cellulosebinding protein showing both cellulase and xylanase activities, whereas SDS-PAGE zymograms showed 4 bands of cellulases and 5 bands of xylanases. Evidence of a cohesinlike amino acid sequence seemed to indicate that the protein complex shared a direct relationship with the cellulosome of Clostridium thermocellum. The crude enzyme from the strain BT14 showed effective degradation of plant biomass. When grown on corn hulls at pH 9.0 and $60^{\circ}C$ under anaerobic conditions, the strain BT14 produced ethanol and acetate as the main fermentation products.

• 한국인터넷방송통신학회논문지
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• 제15권3호
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• pp.181-189
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• 2015

본 연구의 목적은 초기 창업기업에게 민간자금이 유입되게 하는 방안 연구이다. 정부지원 자금 신청 시 수집된 기업정보의 활용방안 및 기업에게 민간자금이 유입할 수 있도록 생산자인 기업과 소비자인 투자자들을 연결하는 정보공유의 장인 플랫폼을 제시하였다. 경제 활성화 방안 중의 하나인 창업지원정책은 정부의 지원 자금 규모의 한계를 때문에 적극적인 민간의 자금 유입을 통한 지속적인 성장지원이 필요하다 이를 위해 본 연구는 정부가 보유한 기업정보와 기업에 투자하는 창업투자자가 교류할 수 있는 장을 마련하는 것으로 민간자금이 우수한 창업기업에게 흘러 들어 갈 수 있는 환경을 제공하는 플랫폼을 제안 하였다. 본 연구는 창업지원 등 관련연구 및 창업활성화에 기여할 것이다. 향후 연구방향은 창업기업 지원을 위한 자금지원이외의 다양한 지원 방안연구가 필요하다.

• 정보보호학회논문지
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• 제25권3호
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• pp.493-500
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• 2015

정부 3.0 공공정보 공유 및 개방, 소셜네트워크서비스의 활성화 그리고 사용자 간의 공유 데이터 증가로 인터넷상에 노출되는 사용자의 개인 정보가 증가하고 있다. 이에 따라 프라이버시를 지키기 위한 익명화 알고리즘이 등장하였으며 관계형 데이터베이스에서의 익명화 알고리즘은 k-익명성(k-anonymity)을 시작으로 ${\ell}$-다양성(${\ell}$-diversity), t-밀집성(t-closeness)으로 발전하였다. 익명화 알고리즘의 성능 향상 부분은 계속해서 효율적인 방법이 제안되고 있지만, 기업이나 공공기관에서는 알고리즘 성능의 향상보다는 전체적인 익명화 처리 방법이 필요한 실정이다. 본 논문에서는 관계형 데이터베이스에서 데이터의 그룹화를 이용하여 k-익명성, ${\ell}$-다양성, t-밀집성 알고리즘을 처리하는 과정을 구체화하였다.

• 한국실내디자인학회논문집
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• 제24권5호
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• pp.117-127
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• 2015

This paper depicts a case study of the BIM (Building Information Modeling)-enabled evaluation of design alternatives for an actual remodeling project in Yeongwol, Korea. The increase of urban population and income followed by the growth of economy has derived massive supply of grand scale housing project in Korea since 1970s. Consequently, building remodeling became one of the feasible resolutions for renovating such old housings in these days. This paper aims to introduce a technical approach to such remodeling projects based on BIM-enabled applications focusing on quantitative analysis of design alternatives. Among the technical issues of such building remodeling projects, this paper focuses on the BIM-enabled area analysis and comparison between design alternatives to support decision-making even in early phase of remodeling design process. BIM and its variety of applications have broadly influenced the domain of AEC-FM (Architecture, Engineering, Construction, and Facility Management) within the lifecycle of buildings. As one of the applications facilitated by BIM, the automated area calculation and scenario-based comparison between alternatives can play an important role in the early phase of remodeling project. We modeled three design alternatives (buildings) and three housing modules (units) based on the actual case in Yeongwol city, Korea using a BIM design authoring tool. Nine combinatorial BIM models were demonstrated for the BIM-enabled review process described in this paper. To determine the most optimal design scenario among nine alternatives, this paper demonstrates a result of the conducted spatial program review. The main subject includes specific spatial program issues on; 1) the number of unit spaces; and 2) area of individual/grouped and private/shared spaces.

• Journal of Microbiology and Biotechnology
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• 제22권7호
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• pp.889-893
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• 2012

Appressorium is a specialized infection structure of filamentous pathogenic fungi and plays an important role in establishing a pathogenic relationship with the host. The Egh16/Egh16H family members are involved in appressorium formation and pathogenesis in pathogenic filamentous fungi. In this study, a homolog of Egh16H, Magas1, was identified from an entomopathogenic fungus, Metarhizium acridum. The Magas1 protein shared a number of conserved motifs with other Egh16/Egh16H family members and specifically expressed during the appressorium development period. Magas1-EGFP fusion expression showed that Magas1 protein was not localized inside the cell. Deletion of the Magas1 gene had no impact on vegetative growth, conidiation and appressorium formation, but resulted in a decreased mortality of host insect when topically inoculated. However, the mortality was not significant between the Magas1 deletion mutant and wild-type treatment when the cuticle was bypassed by injecting conidia directly into the hemocoel. Our results suggested that Magas1 may influence virulence by affecting the penetration of the insects' cuticle.