• The Korean Journal of Mycology
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• v.49 no.1
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• pp.109-118
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• 2021

In this study, we analyzed the diversity of ectomycorrhizal (ECM) fungal communities of Quercus spp. roots in the limestone area. We identified 45 generd of ECM using next generation sequencing (NGS) analysis. Soil chemical composition analysis confirmed soil pH, substitution calcium concentration, total nitrogen content, organic phosphate, and organic matter content. Shannon's Index was calculated according to the changes in soil chemical composition. The results of cluster analysis showed that Sebacina, Tomentella, Tuber, Densospora, Inocybe, Suillus, and Piloderma were the main genera of symbiotic ECM fungi that thrived in soil with high pH and calcium content.

• The Korean Journal of Mycology
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• v.42 no.1
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• pp.34-39
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• 2014

In this study, the community structures of arbuscular mycorrhizal fungi (AMF) in rhizospheres of Camellia japonica and neighboring woody plants in Wando, Korea were investigated. Rhizospheres of C. japonica and other woody plants were dominated by the same species, Acaulospora mellea, but Shannon's index, species richness and total spore numbers of the AMF communities were higher in non-C. japonica than in neighboring plants. Regardless of host plant species, the frequency of A. mellea was significantly high comparing with other AMF species. The community similarity of AMF within C. japonica was significantly higher than between C. japonica and neighboring plants or neighboring plants (p<0.005). Results showed that AM fungal communities in rhizospheres of C. japonica have unique community structure and are different from that of neighboring host plants, suggesting that community structure of AMF could be influenced by host plant species.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.4
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• pp.29-44
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• 2016

This study was carried out to the structure of plant community, and ecological succession sere of forest ecosystem in Soguemgang Valley, Odaesan National Park. Fifteenth plots(size is $20m{\times}20m$) were set up and the results analyzed by DCA which is one of the ordination technique showed that the plant communities were divided into seven groups which area community I(Quercus variabilis-Pinus densiflora community), II(Pinus densiflora community), III(Pinus densiflora-Quercus variabilis community), IV(Pinus densiflora-Quercus serrata community), V(Quercus serrata community), VI(Pinus densiflora-Deciduous broad-leaved plant community), VII(Cornus controversa-Carpinus laxiflora community). Shannon diversity index per $400m^2$ was to 0.7777 to 1.1440 and the age of Pinus densiflora 86 years old, Quercus variabilis was ranged from 66 to 87 years old, Quercus serrata was ranged from 51 to 62 years old, Carpinus laxiflora was 94 years old. In 2013, the succession trend was predicted Pinus densiflora${\rightarrow}$Quercus variabilis, Quercus serrata ${\rightarrow}$Cornus controversa, Carpinus laxiflora. The ecological sucession progress has declined power of the Pinus densiflora and the increased power of the deciduous broad-leaved and Quercus spp. in Soguemgang Valley, Odaesan National Park. Quercus serrata and Quercus variabilis communities that judged Pinus densiflora were progressing by direction of landform. The southern slopes vegetation were progressing for Quercus variabilis, the northern slopes vegetation were progressing for Quercus serrata. In flat Valley, mainly native species of Cornus controversa, Carpinus laxiflora are predicted ecological succession for deciduous broad-leaved tree community.

• Ocean and Polar Research
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• v.33 no.1
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• pp.85-97
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• 2011

The tidal flats of Korea today have reduced by 40% in size compared to 1964. To manage this important habitat properly, development of well-organized and nationwide-applicable grading systems is required. There have been several assessment systems proposed previously in Korea, but they are critically flawed in that selected biological indicators are not adequate and grading criteria are obscure and arbitrary. We reviewed the indicators used in these previous evaluation systems (e.g., diversity indices, quantity and quality of benthic macrofauna, halophytes, water birds, etc.) and subsequently proposed new indicators and an improved grading scheme. For the quantitative assessment of macrobenthic community, biomass reflecting production and ecosystem function is recommended over density, which is much less discriminatory among habitats. Of biodiversity indices used, within-, between-habitat and regional biodiversity indices that accurately reflect sampling efforts are suggested. In addition, we proposed to include species rarity, ecosystem engineers, and the ecological quality index ISEP (Inverse function of Shannon-Wiener Evenness Proportion). As for halophytes, their low spatial coverage on benthic habitat suggests that their presence can be used as an ecological indicator of benthic habitat, regardless of their protective status. We stress the need to introduce 1) quantile approach for quantitative indicators (e.g., diversity, biomass, etc.) in relation to grading, 2) presence-absence approach for spatial or aggregate indicators (e.g., boundaries of halophytes and feeding ground of water birds) and 3) benthic habitat mapping that combines all of these indicators.

• Journal of Microbiology and Biotechnology
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• v.30 no.6
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• pp.804-810
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• 2020

The profitability of the dairy and beef industries is largely affected by the actually achieved reproductive efficiency. Although a large proportion of cows worldwide are bred by artificial insemination (AI) services, many potential factors affecting the outcome of pregnancy by AI remain to be addressed. In the present study, we investigated the vaginal microbiota by high-throughput sequencing of 16S rRNA gene and analyzed their association with differential pregnancy outcomes (i.e., pregnant vs. nonpregnant) of multiple AI services in dairy cows. Sequencing of the V3-V4 region totally produced 512,046 high-quality sequences that were computationally clustered into 2,584 operational taxonomic units (OTUs). All OTUs were taxonomically assigned to 10 bacterial phyla. There were statistically significant differences among the three AI service times (T1, T2 and T3) with respect to the Shannon index and number of observed OTUs (p < 0.05). Bray-Curtis distance-based PCoA analysis also revealed that T2 group could be significantly distinguished from T1 and T3. However, no significant difference between the pregnant and nonpregnant cows was found in confidence regarding both alpha diversity and beta diversity. These results could help us better understand the possible influence of vaginal microbial community on pregnancy outcomes of AI service in cows.

• Journal of Ecology and Environment
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• v.38 no.1
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• pp.95-107
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• 2015

Because an ecological community consists of diverse species that vary nonlinearly with environmental variability, its dynamics are complex and difficult to analyze. To investigate temporal variations of benthic macroinvertebrate community, we used the community data that were collected at the sampling site in Baenae Stream near Busan, Korea, which is a clean stream with minimum pollution, from July 2006 to July 2013. First, we used a self-organizing map (SOM) to heuristically derive the states that characterizes the biotic condition of the benthic macroinvertebrate communities in forms of time series data. Next, we applied the hidden Markov model (HMM) to fine-tune the states objectively and to obtain the transition probabilities between the states and the emission probabilities that show the connection of the states with observable events such as the number of species, the diversity measured by Shannon entropy, and the biological water quality index (BMWP). While the number of species apparently addressed the state of the community, the diversity reflected the state changes after the HMM training along with seasonal variations in cyclic manners. The BMWP showed clear characterization of events that correspond to the different states based on the emission probabilities. The environmental factors such as temperature and precipitation also indicated the seasonal and cyclic changes according to the HMM. Though the usage of the HMM alone can guarantee the convergence of the training or the precision of the derived states based on field data in this study, the derivation of the states by the SOM that followed the fine-tuning by the HMM well elucidated the states of the community and could serve as an alternative reference system to reveal the ecological structures in stream communities.

• Journal of Life Science
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• v.17 no.1 s.81
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• pp.24-30
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• 2007

Inter simple sequence repeat (ISSR) markers were performed in order to analyse the genetic relation-ships of both taxa of Gardenia jasminoides var. radicans and G. jasminoides for. grandifora. Over the 88 fragments, only one locus (ISSR-11-05) was specific to G. jasminoides var. radicans and only one (ISSR-09-05) G. jasminoides for. grandiflora. Although G. jasminoides var. radicans showed low levels of alleles and Shannon's information index than G. jasminoides for. Grandiflora, however, there was not significant differences (p > 0.05). For both taxa the mean genetic diversity of natural populations was higher than that of cultivation populations. It was suggested that domestication processes via artificial selection do not have eroded the high levels of genetic diversity. ISSR markers were more effective in classifying natural populations of wild G. jasminoides in East Asia as well as cultivated G. jasminoides. The information about the phylogenetic relationship of G. jasminoides var. radicans and its closely related species is very valuable of the systematics of genus Gardenia, the origin of cultivated G. jasminoides, and future G. jasminoides breeding.

• Korean Journal of Environment and Ecology
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• v.3 no.1
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• pp.28-41
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• 1989

A survey of Hongryu-Dong and Chi-in district. Gaya National Park, was conducted using 40 sample sites of 500$m^2$ size. TWINSPAN classification confirmed a complex pattern of both local and geographical variation in the vegetation: Dry and wet community types. Within dry community types, two floristic assocation of Pinus densiflora were defined according to local variation. Within wet community types. two floristic association were defined according to altitude. Those associations can be further subdivided floristically into eight subassociation. The vegetation pattern presented by DCA ordination corresponds to one of TWINSPAN at the first two division. The DCA ordination was successful in separating Pinus densiflora from broad leaf forest. Ordination of samples produced arrangements reflectly environmental gradient of soil. The correlation between the first axe of DCA and soil moisture, soil acid, altitude, maximum species diversity and species diversity was significantly negative. The similarity index between each community was very low level.

• Korean Journal of Environment and Ecology
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• v.11 no.4
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• pp.493-505
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• 1998

To study vegetation structure and distance between trees of Pinus densiflora forest in Chayang-chon area, Soraksan National Park, thirty-nine plots(10m$\times$10m) were established. They were classified into two communities by TWINSPAN and DCA technique. In the Community I, P. densiflora(DBH 35~75cm, height 9~16m, age 60~80) dominated in canopy and were predicted to be changed by deciduous broad-leaved trees also. In the Community II, P. densiflora(DBH 9~26.5cm, height 9~16m, age 30~50m) dominated in canopy. It seemed that the community II would maintain P. densiflora community for a long time since P. densiflora dominated in both canopy and understory. Shonnon's diversity index was higher in the community II(1.4247) than in the community I(1.2978). The distantes between canopy trees were 4.92$\pm $2.14m in the community I and 2.41$\pm $0.97m in the community II. The regression between DBH in canpy and ecological distance was Y(Distance) = 0.06355$\times $(DBH)+1.51613.

• Korean Journal of Environment and Ecology
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• v.29 no.2
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• pp.250-262
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• 2015

This study aims to investigate the characteristics of the vegetation structure in the sectin stretching between the Jilmoi wetlands and the Donghae Observatory and to set the criteria for the basic data for a management plan including restoration afterwards. 12 plots($10m{\times}40m$, $20m{\times}20m$) were set up to analyse the vegetation structure. The analysis of the classification by TWINSPAN and ordination by DCA, importance percentage and property, distribution of diameter of breast height, growth increments of major woody species, species diversity and the physicochemical properties of soil were all analyzed. Vegetation classes were divided into 3 communities, which are community I (Pinus densiflora community), community II (Quercus mongolica community) and community III (Quercus mongolica-Tilia amurensis community). The P. densiflora community declined when competing with Q. mongolica and Fraxinus rhynchophylla and Q. mongolica competed with T. amurensis on an understory layer in Q. mongolica community. Q. mongolica competed with T. amurensis on both canopy and understory layers in Q. mongolica-T. amurensis community. P. densiflora declined and it was assumed to succeed to F. rhynchophylla or T. amurensis through Q. mongolica based on the importance percentage and distribution of the diameter of the breast height of small and middle sized trees. The age of P. densiflora was between 47 to 51 years old and Q. mongolica was 61years old. T. amurensis was 61 years old and the growth of Q. mongolica slowed a little. As the result of Shannon's index of species diversity, community I ranged from 0.9578 to 1.1862, community II ranged from 0.7904 to 1.2286 and community III ranged from 0.8701 to 1.0323. The contents of organic matter and cation were low compared to uncultivated mountain soil and it were analysed to be inappropriate for tree growth.