• Journal of Microbiology and Biotechnology
• /
• 제5권6호
• /
• pp.364-369
• /
• 1995

Effect of two-phase culture on Mentha piperita cell growth and essential oil formation was investigated using shake flask and air-lift bioreactors. LiChroprep RP-B(RP-B) addition did not impair M. piperita cell growth, but resulted in stimulated formation of essential oils and increased ratios of extracellular oil to intracellular oil formation. However, the combined use of RP-B and chitosan elicitor was not synergistic. Volumetric productivity of essential oils in RP-B treated culture using cell-recycled air-lift bioreactor was $6.9\;\mu\textrm{g}/l{\cdot}day$ which was substantially higher than that obtainable from the control. Our results demonstrate the potential of a second phase to enhance overall productivity for M. piperita cell culture.

• 한국균학회지
• /
• 제27권1호통권88호
• /
• pp.1-9
• /
• 1999

느타리버섯 8품종의 균사생장은 $25{\sim}30^{\circ}C$의 온도 범위와 $5.5{\sim}6.5$의 pH 범위에서 가장 우수하였다. 영양원의 선발시험에서 탄소원으로서는 황백당, 밀가루 및 옥수수가루가, 그리고 질소원으로서는 대두분이 공시균주의 균사생장이 가장 우수하였다. 농업적으로 이용성이 우수한 황백당 3%, 대두분 0.3%, 인산칼륨 0.05%, 황산마그네슘 0.05%를 배지 조성으로 하는 농업용 액체배지를 선발하였다. 삼각플라스크 배양에서는 250 ml의 erlenmeyer flask에서 보다는 250ml의 shake flask에서 직경 6 mm의 균사절편을 2개 접종하여 배양액량 100ml에서 배양했을 때 공시균주의 균사체 생산이 우수하였다. 그리고 erlenmeyer flask에서 배양방법을 달리하여 느타리버섯의 균사를 배양하였을 때, $45^{\circ}$ 경사배양한 처리구에서 균사체 생산이 가장 우수하였으며, 길이 $50mm{\times}$ 직경 10mm의 유리막대를 넣고 배양한 처리구에서는 균체량이 가장 적었지만 펄프형의 균사생육을 유도할 수 있었다.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제3권1호
• /
• pp.44-47
• /
• 1998

Maximum cellulase production was sought by comparing the activities of the cellulases produced by different Trichoderma reesei strains and Aspergillus niger. Trichoderma reesei Rut-C30 showed higher cellulase activity than other Trichoderma reesei stains and Aspergillus niger that was isolated from soil. By optimizing the cultivation conditions during shake flask culture, higher cellulase production could be achieved. The FP(filter paper) activity of 3.7U/ml and CMCase (Carboxymethylcellulase) activity of 60U/ml were obtained from shake flask culture. When it was grown in 2.5L fermentor, where pH and DO levels are controlled, the enzyme activities were 133.35U/ml (CMCase) and 11.67U/ml(FP), respectively. Ammonium sulfate precipitation method was used to recover enzymes from fermentation broth. The dried cellulase powder showed 3074.9U/g of CMCase activity and 166.7U/g of FP activity with 83.5% CMCase recovery.

• Journal of Microbiology and Biotechnology
• /
• 제14권5호
• /
• pp.944-951
• /
• 2004

The influences of inoculum size, pH, and medium composition on mycelial growth and exopolysaccharides (EPS) production were investigated in shake flasks and in a bioreactor. The optimum inoculum size for both mycelial growth and EPS production was identified to be 10% (v/v) in shake flask cultures. The optimal initial pH for mycelial growth and EPS production in shake flask cultures were found to be 5.0 and 7.0, respectively. However, the optimal pH was 5.0 for both mycelial growth and EPS production in bioreactor cultures where the pH was regulated. The optimal mass ratio of the two major carbon sources, glucose to dextrin, was 1:4. The optimal mass ratio of the two major nitrogen sources, yeast extract to soy tone peptone, was 2:1. When 500 mg $1^{-1}$ of $MnSO_4-5H_2O$ was added to the bioreactor culture, both mycelial growth and EPS production were enhanced by approximately 10%. Under the optimized conditions, a mycelial biomass of 9.85 g $1^{-1}$ and an EPS concentration of 4.92 g $1^{-1}$ were obtained in 4 days.

• Journal of Plant Biotechnology
• /
• 제35권2호
• /
• pp.155-161
• /
• 2008

본 연구는 더덕의 부정근 배양조건과 생물반응기를 이용한 대량생산 체계를 확립하고자 수행하였다. 먼저 기내 식물체를 이용하여 잎, 줄기와 뿌리로부터 부정근을 유도하였다. 부정근의 유도는 줄기에서 가장 높은 결과를 보였다. 또한, 오옥신 종류별 (NAA, IBA와 IAA) 처리시 절편당 부정근 유도수는 1.0 mg/L IBA에서 $9.9{\pm}1.2$개로 가장 양호 하였다. 그렇지만 유도된 부정근의 길이생장은 0.1 mg/L IBA에서 가장 활발하였다. 최적조건의 액체배지 조성을 선발하고자 생체중 0.2 g의 부정근을 각각 MS, 1/2 MS 및 1/3 MS 액체배지가 든 250 mL 삼각플라스크에 접종한 결과 1/2 MS 배지에서 가장 양호한 생장을 보였다. 액체배지조건에서 진탕배양된 더덕 부정근으로 5 L 용량의 공기부양식 생물반응기에 4주간 배양한 결과 1/2 MS에 1.0 mg/L IBA가 첨가된 배지에서 대조구에 비해 16배의 증식이 이루어졌다. 본 연구에서는 더덕 식물체를 이용하여 부정근을 유도 및 증식에 필요한 배양 조건을 조사하였고, 유도된 부정근을 공기부양식 생물반응기에서 대량증식 할 수 있었다.

• KSBB Journal
• /
• 제13권5호
• /
• pp.491-496
• /
• 1998

The production of fibrinolytic enzyme from Bacillus subtilis BK-17 was studied in the shake flask cultures. The important medium components studied include nitrogen source, carbon source and inorganic salts. The environmental conditions include initial pH, temperature, shaking speed and working volume. Among various N-sources, C-sources and inorganic salts tested, soybean flour, D-glucose and Na2HPO4 gave the best results, and their optimal concentrations were 1.5%, 0.5% and 0.05%, respectively. The optimal pH and temperature were 9.0 and 37$^{\circ}C$. With decreasing working volume in the range of 25∼100ml in the 250ml flask or increasing shaking speed in the range of 100∼300rpm, the enzyme production was greatly enhanced. The enzyme activity under the optimal conditions was about 1400I.U./ml with urokinase as a standard.

• Journal of Microbiology and Biotechnology
• /
• 제8권3호
• /
• pp.208-213
• /
• 1998

The effect of the addition of wheat bran to the growth medium on the production of cellulolytic enzymes of Trichoderma reesei Rut C30 was studied in batch culture using shake flasks. The activity of cellulase was enhanced by the addition of wheat bran to the cellulase production medium. $KH_2PO_4$-$K_2HPO_4$ buffer was used for pH control during cellulase production. As a result, high cellulase activities were obtained in shake flask culture; a CMC (carboxymethyl cellulose) activity of 125.78 U/ml was obtained from 2% Avicel- and 3% wheat bran-containing medium and an FP (filter paper) activity of 12.85U/ml was obtained from 1% Avicel- and 5% wheat bran-containing medium after 6 days of cultivation.

• KSBB Journal
• /
• 제8권3호
• /
• pp.295-299
• /
• 1993

Shake flask를 사용하여 M. piperita 세포의 현탁 배양에서의 fungal elicitor, pluronic F-B8, methylcellulose의 영향에 대하여 연구하였다. 그 결과 Rhodotorula rubra라는 균주에서 추출한 fungalelicitor를 처리하여 약 2배 정도 박하정유 생산의 증가를 관찰하였고 100 rpm의 교반속도에서 낮은 농도의 Pluronic F-68, methylcellulose 첨가에 의해 박하세포의 성장이 증진되었다.

• 생명과학회지
• /
• 제14권3호
• /
• pp.472-477
• /
• 2004

야생 균주 Xanthophyllomyces dendrorhous으로부터 biomass와 astaxanthin 산물을 증가시키기 위해 shake flask배양 조건에서 astaxanthin산물에 영향을 미치는 물리적 인자, 영양 인자와 Carotenogenesis의 생성 자극 인자에 대한 연구를 HPLC분석 방법을 이용하여 수행하였다. 최적에 온도, 초기 pH값, 탄소와 질소 공급 조건에서 Carotenogenesis의 선구 물질인 acetic 산, mevalonic 산, 토마토 추출물과 당근 추출물은 astaxanthin 생산을 상당히 증가시킬 수 있었다.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제11권3호
• /
• pp.223-229
• /
• 2006

In this study, we have conducted kinetics and modelling studies of Centella asiatica cell growth and substrate uptake, in an attempt to evaluate cell growth for a better understanding and control of the process. In our bioreactor cultivation experiment, we observed a growth rate of 0.18/day, a value only 20% higher than was seen in the shake flask cultivation trial. However, the observed maximum cell dry weight in the shake flask, 10.5g/L, was 14% higher than was achieved in the bioreactor. Ninety seven percentage confidence was achieved via the fitting of three unstructured growth models; the Monod, Logistic, and Gompertz equations, to the cell growth data. The Monod equation adequately described cell growth in both cultures. The specific growth rate, however, was not effectively predicted with the Logistic and Gompertz equations, which resulted in deviations of up to 73 and 393%, respectively. These deviations in the Logistic and Gompertz models may be attributable to the fact that these models were developed for substrate-independent growth and fungi growth, respectively.