• Korean Journal of Fisheries and Aquatic Sciences
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• v.25 no.5
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• pp.413-431
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• 1992

Gonadal development, fertilization and egg development in the maternal body and reproductive cycle of ovoviviparous rockfish, Sebastes inermis, were investigated histologically. Gonadosomatic index(GSI) of male and female were increased from September and reached maximum values in December. In the male, GSI decreased from January, but in the female maintained high values till February and decreased from March. Hepatosomatic index(HSI) was related to GSI conversely. In both sex, HSI increased from February and reached maximum in August as the gonad were degenerating and resting, and began to decrease from September as gonad were glowing. This ovoviviparous rockfish copulates in December. Fertilization with sperms maintained between ovulated oocytes in the ovary occurs in January mainly. Egg development in the ovarian cavity and discharging of hatched preiarva occurs from January to February. The reproductive cycle includes the successive stages: Growing(September), Mature (October-November), Ripe and Fertilization(Decembr-Janua), Egg development and Discharging of hatched larva(January-February), Degeneration and Resting(February-August). According to the frequency distribution of egg diameter and histological observation, the ovoviviparous rockfish discharged the prelarva at a time in a spawning season. The sexual maturation is first attained at 2 ages. All females and males reaches first maturity at body length of 17.1cm and 15.1cm respectively. The mean number of the embryos increased with the increase of the total length of female.

• The Korean Journal of Malacology
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• v.27 no.3
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• pp.261-271
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• 2011

The gametogenic cycle, the spawning season and the biological minimum sizes in female and male Protothaca (Notochione) jedoensis were investigated by quantitative statistical analysis. In females, monthly changes in the percents of the follicle areas to the ovarian tissue areas and the percents of the oocyte areas to the ovarian tissue areas increased in February and reached the maximum in April, and then gradually decreased from May to July, with the spawning peak between June and July. In males, monthly changes in the percents of the testicular tissue areas to total tissue areas and the percents of the spermatogenic stage areas to the testicular tissue areas increased in February and reached the maximum in April, and then showed a rapid decrease from May to July. From these data, it is apparent that the number of spawning seasons in female and male P. (N.) jedoensis occurred once a year, from May to July. Therefore, P. (N.) jedoensis in both sexes showed a unimodal gametogenic cycle during the year. Compared the gametogenic cycle by quantitative statistical analysis in 2007 with the previous qualitative results of this species, the results of the gametogenic cycle calculated by quantitative statistical analysis showed some differentiations in the spawning seasons evaluated by the gonad index by qualitative histological analysis. The intervals of the beginning of two spawning seasons showed one month between the results of quantitative and qualitative analyses. The biological minimum sizes (considering to 50% of group sexual maturity) in female and male clams by quantitative analysis of this species are 32.01 mm in shell length in females and 30.58 mm in males, respectively. According to the mean shell length fitted to von Bertalanffy's equation, 30.58 and 32.01 mm in shell length were considered to be two years old. Therefore, we assume that both sexes of this population begin reproduction from two years of age.

• Korean Journal of Poultry Science
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• v.19 no.4
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• pp.187-195
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• 1992

This study carried out to investigate selection response of pure line population of layer chicken. Data for two layer pure line of Single Comb White Leghorn were collected from 1986 to 1991. Primary selection criteria were egg number to 270 days of age (EN270), egg weight(BW270), body weight (BW270) at 270 days of age and the days of the 1st egg(SM). The results obtained in this study are summarized as follows ; 1. Mean phenotypic values of two strains were decreased by 1.94 day(K), 1.320ay(F) for SM and increased by 0.09g(K), -0.05g(F) for EW 270 and 0.56(K), -0.78(F) for EN270. 2. The ratio of a realized selection differential to expected selection differentials for SM (sexual maturity), EW 270, and BW 270 were not significantly decreased during 5 generations. 3. Heritability were estimated from sib analysis. The means of estimates were 0.28(F), 0.39(K) for BW270, and 0.25(F), 0.29(K) for EN270 and 0.51(F), 0.48(K) for EW270. 4. The genetic gains were estimated by method of selection index in retrospect on the basis of selection differentials. EN270 and EW270 were found to increase by 2.01(F), 2.25(K) and 0.54 (F), 0.65(K) per generation.

• Parasites, Hosts and Diseases
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• v.37 no.1
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• pp.21-26
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• 1999

The growth and development of Gymnophalloides seoi were studied in C3H/HeN mice and effects of immunosuppression of the host on the worm development were observed. Two hundred metacercariae of G. seoi were orally administered to each mouse, and worms were recovered on days 1, 3, 5, 7, 14 and 21 post-infection (PI). The worm recovery rate was significantly higher in immunosuppressed (ImSP) mice than in immunocompetent (ImCT) mice except on days 1 and 3 PI. The worms attained sexual maturity by day 3 PI with eggs in the uterus, and worm dimensions and the number of uterine eggs continuously increased until day 14 PI in ImSP mice. Worms recovered from ImSP mice were significantly larger in size than those from ImCT mice on days 1 and 3 PI, and the number of uterine eggs was significantly larger in ImSP mice on days 5 and 7 PI. Genital organs such as the ovary, testes, and vitellaria, that were already developed in the metacercarial stage. grew a little in size until day 14 PI. The results show that the C3H/HeN mouse is, though not excellent, a suitable laboratory host for G. seoi.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.40 no.1
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• pp.9-16
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• 2004

Parapenaeopsis tenella occurs widely on the south and west coasts of Korea. Sex ratio showed seasonal variations, with a mean value of 48.6% for the females. The species produces one cohort a year, with the ovaries ripening from July to August. Insemination took place from July to August, as more than half of the females sampled in the study over 14 mm CL were inseminated. Gonadosomatic index (GSI) reached the maximum between July and August. The smallest mature female was 11 mm CL. Size at 50% sexual maturity ($CL_{50}$), determined from both mature females and inseminated females, was 12.53 mm and 12.28 mm CL, respectively. The life span of females appeared to be 14-15 months according to size frequency distributions, while that of the male was 13-14 months. Population growth was estimated by the modified von Bertalanffy growth function incorporating seasonal variation in growth. Based on the growth parameters (K = 1.22 $yr^{-1}$ and $L_{\infty}$ = 21.99 mm CL for females, and K = 2.00 $yr^{-1}$ and $L_{\infty}$ = 15.00 mm CL for males) growth curves showed that females grew faster and reached a larger size at age than males.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2001.11a
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• pp.71-73
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• 2001

In this study, we could improve transmission efficiency of germline chimeras by transfer of gonadal PGCs (gPGCs) cultured in vitro. Of hatched recipient chicks, 301 chickens (141 males and 160 females) were brought up to sexual maturity and these WLs (KOC) were mated with KOCs for testcross, resulting in 27 germline chimeras (15 males and 12 females) identified by black feather color of their progenies. The production efficiency of germline chimera production of experimental groups was observed (P=0.6831). The average transmission efficiency of proven germline chimeras was 0.6 ∼56.5% (15.0% on average). The transmission efficiency of experimental group which were transferred 10-days cultured gPGCs without Ficoll treatment was highest (49.7%) and that of experimental stock which transferred non-cultured gPGCs with Ficoll treatment was lowest (0.6%). The duration of in vitro culture before transferring was significantly important for the high efficiency of germline transmission. Transferring 10-days cultured gPGCs made the transmission efficiency higher rather than transferring non-cultured and 5-days cultured gPGCs, 50 times and 10 times, respectively (p<0.0001). However, Ficoll treatment for increasing the population ratio of gPGCs negatively affected the transmission efficiency and the effects of sexuality and the reciprocal interaction between treatments showed no significant differences. These findings demonstrated that the crucial factors for improving the germline transmission were the duration of in vitro culture prior to transfer. Thus, we developed the complete system for production of germline chimera using cultured gPGCs with highly improved efficiency and this system would be useful for genetic manipulation and obtaining the transgenic aves.

• Parasites, Hosts and Diseases
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• v.26 no.3
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• pp.179-188
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• 1988

The life cycle of Fibricola seoulensis was studied in the laboratory and in the field, with special interests in the larval developments within the eggs and in the intermediate hosts. The first emergence of miracidia after incubation of eggs in 26C water began on the ninth day. The miracidia, elongate and cylindrical shape, had epidermal plates in the formula of 6, 9, 4 and 3, with two pairs of flame cells and lateral processes. A kind of fresh water snail, Hippeutis (H.) cantori, was found to shed furcocercous cercariae from the 13th day after experimental challenge with miracidia while Physa acute failed to shed. The same kind of snail collected from the field also shed the same cercariae. The cercariae were equipped with 2 pairs of penetration glands and 5 pairs of fame cells. The tadpoles of Rana nigromaculata were found susceptible to experimental infection with the cercariae. The same kind of tadpoles collected from various areas were also found naturally infected. The metacercariae in the tadpoles which were infected experimentally became infective to the definitive host in 21 days. The metacercariae were located free in the body cavity of tadpoles, and attained sexual maturity in rats in 7 days. The present study successfully followed the complete life cycle of F. seoulensis and found that it is possible to maintain the life cycle in the laboratory.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.39 no.2
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• pp.120-127
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• 2003

In Korea, Trachysalambria curvirostris occurs widely along the south and west coasts with a range extending from Kanghwado in the northwest down to Sarangdo in the southeast. Sex ratio showed seasonal variations, with a mean value of 48.6% for the females. T. curvirostris produces one cohort a year, with the ovaries ripening from July to August. Insemination appeared to take place from June to August, as more than half of the females sampled in the study of over 19 mm carapace length were inseminated. The mean gonado somatic index (GSI) reached a maximum between July and August. The smallest mature female found was a 18 mm carapace length (CL). Size at 50% sexual maturity (CL$_(50)$), determined from both mature females and inseminated females was 18.89 mm and 19.91 mm CL, respectively. The life span of females appeared to be 14-15 months according to size frequency distributions, while that of the male was 13-14 months. Population growth was estimated by the modified von Bertalanffy growth function incorporating seasonal variation in growth. Based on the growth parameters (K = 1.40 yr$_(-1)$ and L$L\infty$ = 29.54 mm CL for females, and K = 2.00yr$_(-1)$ and L$L\infty$ = 18.95 mm CL for males) growth curves showed that females grew faster and reached a larger size than males.

• Journal of the korean academy of Pediatric Dentistry
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• v.37 no.3
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• pp.359-366
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• 2010

It was easy to find that children of a skeletal anterior crossbite in the early mixed dentition period showed a stark difference in the dental maturity between their maxillary and mandibular teeth, if they have stronger physical characteristics. If the difference of dental age between maxillary and mandibular teeth which can be identified via panoramic radiographs may serve as an early sign of class III malocclusion, this is considered valuable as a tool of early detection diagnosis. We obtained lateral cephalometric radiographs, panoramic radiographs, working model and clinical images of patients of Hellman dental age IIA and IIC who visited the department of pediatric dentistry, Pusan National University Dental Hospital and examined them to select 50 patents for normal occlusion group and skeletal anterior crossbite group, respectively. Their panoramic radiographs were used for the Demirjian's method to figure out dental ages of maxillary and mandibular teeth of each group and the eruption rate of the first molars. Their differences are as follows: 1. In both groups, the dental ages from Demirjian's method were advanced than the chronological ages. No sexual dimorphism was detected for the chronological or dental age in either group (p>0.05). 2. The difference of dental age of maxillary and mandibular teeth between the normal occlusion group and crossbite group was 0.22 and 0.69 years, respectively, with a higher difference in crossbite group(p<0.05). 3. Compared to the normal occlusion group, the crossbite group showed a higher difference in the eruption rate between maxillary and mandibular first molar(p<0.05).

• Korean Journal of Agricultural Science
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• v.12 no.1
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• pp.55-61
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• 1985

This study was carried out to investigate reproductive traits and sex hormone levels by growth in Korean native goats. Serum levels of FSH, LH, prolactin, estradiol-$17{\beta}$ and progesterone were determined every 15 days from the 70 days of age to 190 days by radioimmunoassay. The age and weight at sexual maturity were 183.6 days and 14.3 kg; the length of estrous cycle and estrus period were 20.3 days and 36.7 hours, respectively. The gestation period, litter size and body weight at birth were 148.4 days, 1.4 head and 1.8 kg, respectively. The levels of serum LH were highest with 3.93 mIU/ml at 70 days of age, thereafter decreased gradually to 1.21 mIU/ml at 190 days of age. The concentrations of FSH in serum were below 1.25 mIU/ml throughout the experimental period. The levels of prolactin were lowest with 3.09ng/ml at 85 days of age and highest with 4.65 ng/ml at 175 days of age. The serum levels of estradiol-$17{\beta}$ were increased with age, thus highest with 7.95 pg/ml at 190 days of age. The serum progesterone concentrations were maintained low levels (below 1.0 ng/ml) throughout the experimental period.