• Title/Summary/Keyword: Sex identification

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Occurrence of Grapholita dimorpha in Korean Pear Orchards and Cross-trapping of Its Sibling Species, Grapholita molesta, to a Pheromone Lure (국내 배과원에 복숭아순나방붙이의 발생과 유사종 복숭아순나방의 페로몬 트랩 교차 유인)

  • Jung, Chung Ryul;Ahn, Jeong Joon;Eom, Hoon Sik;Seo, Jung Heun;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.479-484
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    • 2012
  • The occurrence of plum fruit moth, Grapholita dimorpha, has been recently reported in apple orchards in Korea. It has been suspected that G. dimorpha and its related species, G. molesta, may occur simultaneously in other host plants. This study reports the occurrence of G. dimorpha in pear orchards of different localities in Korea. The identification of G. dimorpha was determined by morphological characters and the DNA marker. The cross-trapping of both species may be possible because the major sex pheromone (SP) compositions for the two species are similar. From the monitoring data, G. dimorpha and G. molesta were caught in SP lure traps of G. dimorpha and both species were also caught in SP lure traps of G. molesta. This cross-trapping of G. molesta to a SP lure of G. dimorpha varied significantly among pear orchards in different geographical localities. Furthermore, the occurrence peaks of the two species were not coincidental in all monitored orchards. These suggest that monitoring data obtained from each SP trap of both species in pear orchards may be mixed with two species, which would result in the over-estimation of population density and peak frequency on both species in pear orchards.

Development of a Female-associated SCAR Marker in Schisandra nigra Max. (Schisandra nigra Max.에서 암그루에 연관된 SCAR 마커의 개발)

  • Han, Hyo Shim;Jung, Jae Sung
    • Journal of Life Science
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    • v.31 no.6
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    • pp.537-542
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    • 2021
  • Schisandra nigra Max., a dioecious plant native to Jeju Island in Korea, is cultivated on a small scale for fruit production. As fruit-producing female individuals are generally considered to be more valuable than male, early identification of plant sex at the seedling stage is important. In this study, a sequence-characterized amplified region (SCAR) marker associated with a female-specific region in the genome of S. nigra was investigated. Of 120 randomly amplified polymorphic DNA (RAPD) primers, one primer (OPB-03) consistently amplified a 749 bp band in female plants. The female-specific PCR product was isolated and cloned, and the nucleotide sequences were then determined. Southern hybridization performed using the female-specific fragment as a probe produced positive signals only in genomic DNA from the female plants. This result revealed that the 749 bp segment of DNA was present in the genome of female plants but absent in the genome of male plants. A SCAR primer pair was designed based on the RAPD marker to amplify a 436 bp fragment in the genomic DNA of female plants. This primer pair amplified the expected size of DNA fragment in female plants and four monoecious individuals collected from a natural population. The SCAR marker identified in this study can be used to distinguish female-flowering individuals at the seedling stage.

Effects of Early- and Late-Feathering Phenotypes on Growth Performance and Mortality in Korean Native Commercial Chickens (토종 실용닭의 깃털 조만성 형태가 산육능력 및 생존율에 미치는 영향)

  • Sohn, Sea Hwan;Choi, Eun Sik;Cho, Eun Jung;Kim, Bo Gyeong
    • Korean Journal of Poultry Science
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    • v.48 no.4
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    • pp.177-184
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    • 2021
  • Sex identification in day-old chicks is essential in the poultry industry. Currently, the feather-sexing using a sex-linked inheritance is a chick sexing method used extensively in the poultry industry. For chicks produced by feather-sexing, all females are early-feathering (EF) chicks and all males are late-feathering (LF) chicks. Therefore, investigating the effects of EF and LF phenotypes on production traits of chickens is critical. The purpose of this study was to analyze and compare the production performance between EF and LF chickens in Korean native commercial chickens. The results showed that the survival rate of the EF chickens was significantly higher than that of the LF chickens, from hatching to 12 weeks of age (P<0.05), with the highest difference observed in females. However, no significant difference was observed in body weight between the EF and LF chickens at almost all ages. In addition, no significant difference was observed between in feed utility, such as average daily feed intake and feed conversion ratio, between the EF and LF groups. In conclusion, although the survival rate of early-feathering chickens was superior to that of late-feathering chickens, no significant difference was observed in growth performance and feed utility among Korean native chickens. The results suggest that production capacity is not influenced by feathering type in the establishment of a breeder structure and production system for feather-sexing Korean native chickens, which is valuable for the practical application of feather-sexing in the poultry industry.

A Comparison on the General Characteristics of Acute Stroke Patients between Fire-Heat and Deficiency of Yin (화열과 음허로 진단된 급성기 뇌질환 환자의 특성 비교 연구)

  • Kim, Hye-mi;Gwak, Ja-young;Cho, Seung-yeon;Shin, Ae-sook;Lee, In-whan;Kim, Na-hee;Na, Byung-Jo;Park, Seong-Uk;Jung, Woo-sang;Mun, Sang-gwan;Park, Jung-mi;Ko, Chang-nam;Cho, Ki-ho;Kim, Young-suk;Bae, Hyung-sup
    • The Journal of the Society of Stroke on Korean Medicine
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    • v.10 no.1
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    • pp.33-39
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    • 2009
  • Objects : This study propose to compare on general characteristics of acute stroke patients between Fire-Heat group and Deficiency of Yin group. Method : We recruited acute stroke patient within 4 weeks after stroke admitted to Department of Internal Medicine of Kyunghee University Oriental Medicial Center, Dongguk University Ilsan Oriental Medical Center, Kyungwon University Songpa Oriental Medical Centar and Kyungwon University Incheon Oriental Medical Center from April 2007 to August 2009. We investigate age, sex, type of stroke, past history, general characteristics, smoking, drinking alcohol, marriage sasang constitution, etc. Result : It was significant different in sex, smoking, dringking alcohol and marriage between Fire-Heat group and Deficiency Yin group. We need more cases for another result that could associate with diagnosis of stroke. Conclusion : We found that Fire heat group was more associated with lifestyle than deficiency yin group. Further study will be needed to analysis for the diagnosis and the treatments of stroke.

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A Study on the Change of Primipara′s Mother - Infant Interaction by Infant′s Monthly Age (영아의 월령에 따른 초산모의 모아상호작용에 관한 연구)

  • 방경숙;한경자
    • Journal of Korean Academy of Nursing
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    • v.21 no.2
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    • pp.150-167
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    • 1991
  • The intent of this longitudinal descriptive correlational study is to investigate the change of the mother - infant interactions over the periods of one month, three months and six months of the infant's age. This study is also intended to explore the interrelationships among three major factors- the perception of pregnancy, the mother -infant inter-action and the maternal attitude. The samples participated are 36 pairs of mother and infant from two university hospitals in Seoul. A data collection was made, from October in 1988 to September in 1989, for each subject at five periods of time ; prenatal period (after 36 weeks in pregnancy), postpartum two or three days, one month, three months and six months of infant's age. The research tools used in this investigation are Mother - Infant Play Interaction Scale (MIPIS), Prenatal Self- Evaluation Questionnaire and Cohler's Maternal Attitude Scale. Some of the findings are as follows ; 1. There is a significant increase in the mother - infant interaction from one month to three months and six months of infant's age. The highest score of the mother - infant interaction during three periods is at the time of three months. 2. The primipara's mother -infant interaction is not affected by the demographic characteristics, such as age, education and vocation of mother, types of delivery, or sex of infant. 3. Among seven categories of the perception of pregnancy, the most positively perceived categories by primiparas are acceptance of pregnancy and identification of. a motherhood role : whereas the negative ones are preparation for labor, concern for well -being of self and baby, md fear of pain, helplessness and loss of control in order of negativity. 4. There is no significant relationship between the perception of pregnancy and the mother - infant interactions. 5. There is no significant change in the maternal attitude over the period of one month, three months, and six months of infant's age. 6. There is no significant relationship between the maternal attitude and the mother - infant interactions. 7, The significant relationships are (end between the perception of pregnancy and maternal attitude, especially in the categories of concern for well -being of self and baby, accetance of pregnancy, identification of a motherhood role, fear of pain, helplessness and loss of control, and relationship with husband. In conclusion, it is confirmed that primipara's mother-infant interactions change over time period, and that perception of pregnancy and the maternal attitude do not affect the mother-infant interactions despite a significat relationship between those two variables. The implications of this investigation include a suggestion that nurses need to teach and demonstrate to mothers how to care for and interact with their infants, with a view to improving the mother-infant interactions which can be obtained through the improvement of maternal sensitivity to their infants.

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Genetic Stock Identification of Spotted Flounder, Verasper variegatus from Yeocheun, Korea (범가자미에 대한 유전학적 동정)

  • KIM Kyung Kil;KIM Yoon;NAM Yoon Kwan;KIM Dong Soo
    • Journal of Aquaculture
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    • v.6 no.3
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    • pp.221-233
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    • 1993
  • Cell size, DNA content, chromosome and PCR-based mitochondrial 12S rRNA gene analyses were conducted to obtain basic informations for genetic stock identification of spotted flounder (Verasper variegatus) from Yeocheun, Korea. The mean erythrocytic and nuclear volumes of spotted flounder were $211.10{\mu}m^3$ and $23.03{\mu}m^3$, respectively. The haploid DNA content of this species was 0.79 pg/cell which correspond to $46.5\%$ of carp and to $22.6\%$ of mammals. Spotted flounder had the 2n = 46 acrocentric chromosomes but no heteromorphic sex chromosomes was found. Mitochondrial DNA gene for 12S ribosomal RNA was amplified by polymerase chain reaction (PCR) and the PCR products were subjected to digestion with 15 restriction endonucleases. Restriction enzyme analyses revealed that Ava I, Mae II, Sma I and Xba I had one restriction site in the mitochondrial 12S rRNA gene segment of spotted flounder, while Mae I had two. Segments of 12S rRNA gene from mitochondria in spotted flounder were sequenced and compared with channel catfish and human as controls. The 12S rRNA gene of this species was more similar to that of channel catfish than to human's.

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A Study on the Selection of Health topic areas and major concepts for Health Education in Primary and Junior High Schools (초.중학생을 위한 보건교육의 영역 및 주요개념 선정을 위한 일 연구)

  • 이경자
    • Korean Journal of Health Education and Promotion
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    • v.7 no.1
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    • pp.10-26
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    • 1990
  • In Korean education, the health contents are scattered in various course subjects throughtout the primary and junior high school curriculum. So it is very difficult to provide systematic health education. The purpose of this study was to provide a guide for health education using health topic areas and major concepts that represent the scope of material that should be covered in health instruction. The steps used in selecting these health topic areas and major concepts were as follows: 1. A review of the literature related to health and health education was done to develop the rationale underlying this study. 2. Health topic areas basic to the growth and development characteristics of children, to human needs and to societal needs for healthful living were indentified. 3. The major concepts for each health topic area based on health sciences and children's growth and development levels were selected. 4. The major concepts selected were organized in sequence to guide health education from grade one to grade nine. The results of this study were as follows: 1. The identification of eleven health topic areas essential for health education. These include: personal habits and health healthy growth and development nutrition and health prevention of disease and disorders drugs and health mental health family life and health sex education accident prevention consumer health community health 2. The identification of the major concepts(generalizations) for each health topic area: 33 major concepts were identified as a guide in determining the health content of health education programs. These are 1) body cleaniness, 2) health of the sensory organs, 3) dental health, 4) exercise and rest, 5) growth and development, 6) body structure and function, 7) developmental tasks, 8) balanced nutrition, 9) eating habits, 10) food preparation and food storage, 11) sources of disease and disorders, 12) disease preventive behavior, 13) care during illness, 14) drug use and misuse, 15) drug addiction, 16) emotional responses, 17) human relationship, 18) self concept, 19) social adjustment, 20) health habits of the family, 21) interdependence of family members, 22) origin of life, 23) characteristics of man and woman, 24) sexual instinct, 25) safety behavior, 26) emergency measures, 27) criteria for selection of health products, 28) proper use of health information, 29) utilization of health and medical services, 30) environmental conservation, 31) environmental pollution, 32) population control, 33) function of public health services. 3. The organization of the concepts(generalizations) in sequence and for continuity in health instruction at the primary and junior high school level.

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Sentinel Lymph Node Identification Using $^{99m}Tc$-Neomannosyl Human Serum Albumin in Esophageal Cancer; Comparison with $^{99m}Tc$-Phytate (식도암 환자에서 $^{99m}Tc$-Neomannosyl Human Serum Albumin과 $^{99m}Tc$-Phytate를 이용한 감시림프절 탐색의 비교)

  • Kim, Hyun-Koo;Kang, Du-Young;Kim, Seung-Eun;Park, Jong-Jae;Jeong, Jae-Min;Mok, Young-Jae;Choi, Young-Ho
    • Korean Journal of Bronchoesophagology
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    • v.16 no.2
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    • pp.109-114
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    • 2010
  • Background: The aim of this study was to compare a novel mannose receptor-binding agent(Technetium-99m human serum albumin, $^{99m}Tc$-MSA) with $^{99m}Tc$-phytate for sentinel node mapping in patients with esophageal cancer. Material and Method: Twenty patients with clinical stage T1N0m0 or T2N0M0 esophageal cancer that were candidates for esophagectomy were enrolled. Endoscopic injection of $^{99m}Tc$-MSA or $^{99m}Tc$-phytate was administered at the peri-tumor region before surgery in 10 patients. The radioactive lymph nodes were identified with a handheld gamma probe after lymph node dissection. Results: The patient's age and the sex ratio of both groups were similar. The clinical stage, tumor location, and operative technique did not differ. The total number of dissected lymph nodes did not differ ($28.5{\pm}9.12$ in MSA group, $32.2{\pm}11.24$ in phytate group, p=0.430). The sentinel node was identified in all cases in both groups. The number of sentinel nodes per patient was $2.7{\pm}1.57$ in the MSA group, which was significantly greater than the $1.7{\pm}0.88$ in the phytate group (p=0.036). Five out of 20 patients whose sentinel nodes could be identified had metastases; however, neither group had any false-negative results for sentinel node identification. Conclusion: Sentinel nodes were detected more frequently with MSA than with phytate.

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Identification and Characterization of Novel Sequences of ev21-K Locus for Feather-Sexing in Chickens

  • Eun Jung Cho;Sea Hwan Sohn
    • Korean Journal of Poultry Science
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    • v.51 no.2
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    • pp.117-125
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    • 2024
  • This study aimed to find genetic markers for breed-independent identification of early- and late-feathering chickens. We explored the novel sequences of the ev21-K locus associated with late-feathering and investigated its characterization. Additionally, the genetic transmission pattern of the identified sequences were investigated to understand its potential application in auto-sexing lines. A total of 707 chickens from 5 chicken breeds were employed for the study. The ev21-K locus was identified through a comparative analysis of the ev21 gene and the K gene related to feather development. For analysis of identified loci, specific primers for the target sequences were prepared and polymerase chain reaction (PCR) was performed to obtain the products, and then their nucleotide sequences were analyzed. Crossbreeding tests of early-feathering and late-feathering chickens were conducted to examine the genetic transmission patterns of the identified sequences. The results showed that the identified 230 bp ev21-K locus, which named as ev21-related K specific sequences were 99% homology with the ev21 gene. PCR analysis confirmed its presence exclusively in late-feathering chickens. Comparative analyses across tissues, breeds, and ages demonstrated the sequences consistency in identifying late-feathering chickens. Genetic transmission patterns were investigated through crossbreeding tests, revealing sex-linked inheritance and consistent segregation with feathering phenotypes. The inheritance patterns of the ev21-related K specific sequences demonstrated that this locus follows the typical Mendelian inheritance pattern as a dominant gene. In conclusion, the novel sequences of ev21-K locus were a reliable molecular marker for identifying early- and late-feathering chickens across breeds.

Molecular characterization and prenatal molecular evaluation of three fetuses in four unrelated Korean families with Lesch-Nyhan syndrome

  • Yoo, Han-Wook;Kim, Gu-Hwan
    • Journal of Genetic Medicine
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    • v.2 no.1
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    • pp.17-22
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    • 1998
  • The Lesch-Nyhan syndrome which is caused by the deficiency of hypoxanthine guanine phosphoribosyltransferase is an X-linked recessive disorder characterized by hyperuricemia, choreoathetosis, mental retardation and compulsive self-injurious behavior. Clinical management of the patients with the Lesch-Nyhan syndrome is frustrating and requires burdensome medical treatment since it cripples the patient and shortens the life span by progression of neurological symptoms, but there are no cures or measures for relieving relentless natural course of the disease yet. Therefore, prenatal diagnosis of the affected fetus is important in genetic counselling for the family at high risk. In this study, four different mutations in the HPRT gene of four probands have been identified in four unrelated families; K215X, Q109X, nt.631 ${\Delta}A$, and nt.289 ${\Delta}GT$. Two mutations among them altered restriction enzyme sites; SpeI for Q109X and MaeI for nt.289 ${\Delta}GT$. Based on their molecular defects, prenatal diagnoses of 3 the fetuses were successfully made between ninth and eleventh week of gestation by polymerase chain reaction (PCR), restriction digestion and DNA sequencing using cDNA obtained from chorionic villus samples (CVS). We predicted the outcome of all fetuses prenatally. Among the three fetuses two were male and one was female according to the identification made by PCR amplification of the sex determining region of the Y chromosome(SRY) gene. Each carried a wild type allele for the corresponding mutant allele. They were also tested postnatally for the mutations to be unaffected.

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