• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제4권4호
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• pp.154-158
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• 2023

The Asiatic black bear, Ursus thibetanus, is among the most threatened or endangered species in Asia. For its conservation and management, sex identification of U. thibetanus using non-invasive samples (e.g., hair and/or feces) is potentially valuable. In this study, a non-invasive molecular method for sex identification of U. thibetanus samples collected from various countries was first utilized, and it was based on polymerase chain reaction (PCR) amplification of the amelogenin gene via PCRs. Thirty-three bear DNA samples, extracted not only from blood (n=9) but also from hair (n=18) and feces (n=6), were used. We performed sex-specific PCR amplifications of the amelogenin gene using a primer set, SE47 and SE48. The primer set could successfully amplify a single X-specific band for females and both X- and Y-specific bands for males from all blood (100%) and hair (100%) samples. In addition, the primer set could distinguish the sex of bears in four out of a total of six fecal samples (approximately 67%). This study's findings suggest that this molecular method can be applied to sex identification of Asiatic black bears from various Asian regions using non-invasive samples, such as hair and feces.

• Animal cells and systems
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• 제11권2호
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• pp.165-168
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• 2007

The nucleotide sequences of a male-specific marker sex determining region Y (SRY) gene and a mitochondrial cytochrome B (CYTB) gene were characterized and analyzed to establish a molecular method for identification of species and sex of Korean roe deer (Capreolus pygargus tianschanicus). Similarity search result of SRY sequences showed very similar result to those reported in Moose (Alces alces) and Reindeer (Rangifer tarandus), both of which had 95.9% similarity in identity. CYTB genes were very similar to those reported in Siberian roe deer (C. pygargus pygargus) which had 98.6% similarity and not to European roe deer (C. capreolus), suggesting that the DNA samples tested were of Siberian roe deer lineage. Polymerase chain reaction (PCR)-based sex typing successfully discriminated between carcasses of male and female roe deer. Males had SRY band on agarose gels and females did not. The result of this molecular sex typing provided similar information with that obtained by genital organ observation. Therefore, this molecular method using male specific marker SRY and mitochondrial CYTB genes would be very useful for identification of the species and sex of the carcass remains of roe deer.

• 한국응용곤충학회지
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• 제34권1호
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• pp.9-14
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• 1995

From a chemist's perspective, Scarabaeidae is one of the most exciting group of insects to work on the isolation, identification, and synthesis of sex pheromones because-as opposed to Lepidoptera, which by and large utilizes straight chain alcohols, aldehydes, and acetates-the pheromonal chemistry of scarab beetles is remarkably diverse. While species in the subfamily Rutelinae utilize pheromone constituents, which are presumably fatty acid derivatives, the more primitive species in the subfamily Melolonthinae use phenolic, amino acid derivative, and terpenoid compounds. Here, I discuss the recent advances we have accomplished in the identification of scarab sex pheromones with especial emphasis on their chemical diversity. Also, I discuss the potential role of these sex pheromones in insect pest management. Field tests revealed that, in contrast to what has been frequently observed in the Lepidoptera, the higher the dosage of sex pheromone loaded in the traps the greater the capture of scarab beetles. These data suggest that mass trapping is more likely to be useful for scarab pest management than mating distruption.

• Fisheries and Aquatic Sciences
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• 제22권8호
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• pp.18.1-18.5
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• 2019

The development of sex-specific genetic assays in a species provides both a method for identifying the system of sex determination and a valuable tool to address questions of conservation and management importance. In this study, we focused on the identification of single nucleotide polymorphisms (SNPs) that differentiate genetic sex in burbot Lota lota. Burbot are the only true freshwater representative of the cod family and a species of conservation and management importance throughout Eurasia and North America. To identify sex-specific SNPs, we utilized restriction site-associated DNA sequencing (RADseq) to interrogate thousands of SNPs in burbot samples of known phenotypic sex. We discovered 170,569 biallelic SNPs, none of which fit the pattern expected under female heterogamety. However, we identified 22 SNPs that fit the pattern expected under male heterogamety (males heterozygous XY, females fixed XX) and, from these, developed two genetic assays that robustly (~ 97% genotyping success) and accurately (> 99% correct) sexed burbot samples. These sex-specific genetic assays will benefit growing conservation aquaculture programs for this species and allow future assessments of sex-specific migration, growth, and mortality.

• 한국인구학
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• 제20권1호
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• pp.65-96
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• 1997

본 연구의 목적은 1980년대 중반 이후부터 지속되고 있는 한국사회 전반에 걸친 출생시 성비불균형현상 가운데 특히 다른 지역에 비해 불균형 정도가 심각한 대구지역의 출생시 성비불균형의 원인을 경험적으로 규명하는 데 있다. 이러한 목적에서 한국에서 태어났지만 사회적,문화적,환경적 여건이 서로 다른 미국으로 이민하여 캘리포이아의 베이지역에 거주하고 있는 한국동포 기혼여성들의 성차별의식, 아들선호도, 태아성감별, 인공유산 등 출생시성비에 영향을 미친다고 알려진 요인들을 대구지역의 기혼여성들과 비교 분석하였다. 본 연구에는 대구지역과 미국 캘리포니아 베이지역에서 거주하는 25-44세 사이의 기혼여성들을 대상으로 조사한 설문조사 내용을 자료로 사용하였다. 연구결과 대구지역의 기혼여성들이 미국 베이지역의 한국동포 기혼여성보다 성차별을 더 많이 느끼고 있으며 아들선호도 역시 높은 것으로 밝혀졌다. 특히 아들을 낳기 위한 노력의 일환으로 임신 전부터 아들을 낳기 위한 시도는 대구지역 기혼여성들이 베이지역 한국동포 기혼여성들보다 2배 정도 더 높으며 태아의 성감별 후 여아로 판명되어 인공중절수술을 실시한 비율은 무려 여섯 배를 능가하는 것으로 밝혀졌다. 출생시 성비불균형에 결정적인 영향을 미치는 변수들을 경험적으로 규명하기하기 위하여 도입한 로지스틱회귀분석 결과에 의하면 대구지역의 경우 성차별 정도, 아들선호도, 성감별 등은 통계적으로 유의미한 영향을 미치는 것으로 나타났으나 베이지역의 경우 연령만이 통계적으로 유의미한 영향을 미치는 것으로 밝혀졌다. 그러므로 본 연구결과를 통하여 우리는 대구지역의 출생시 성비불균형의 가장 큰 원인은 무엇보다도 타지역에 비해 지나치게 강하게 남아있는 유교적 전통에서 비롯되는 아들선호의식에서 출발하고 있음을 알 수 있으며, 이러한 아들선호는 사회계층, 연령, 교육수준의 차이를 막론하고 존재하고 있는 것으로 보인다.

• 한국임상수의학회지
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• 제37권1호
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• pp.50-52
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• 2020

Twenty-four cinereous vultures that had been taken to a wildlife center due to starvation and exhaustion were studied to evaluate approaches for determining sex. Coelioscopy was performed to identify sexes of two vultures, whereas, DNA testing was performed to identify the sexes of the 24 vultures. Testes and ovaries could be unambiguously identified with an endoscope and DNA analyses could identify sex sex in most, but not all of the specimens. Although the coelioscopy examination can unambiguously confirm sex, the approach is invasive and requires anesthesia. Thus, coelioscopic examination should only be performed when sex cannot be determined through DNA analysis.

• Journal of Oral Medicine and Pain
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• 제19권2호
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• pp.205-219
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• 1994

Cigarette butts from 5 smokers were gathered and then, placed in room temperature for 1, 3, 5, 7, 15 days. The possible use of the cigarette butts for individual identification was evaluated in sex determination, amplification of D1S80 locus, polymorphisms of HLA-DQA1 gene from the extracted DNA. 1. DNA extraction was possible in cigarette butts weree left in room temperature for 15days, so it can be applicatable to individual identification by polymerase chain reaction(PCR). 2. Amplification of X-Y homologous amelogenin gene by PCR made it possible to identify the sex in saliva stains (cigarette butts). 3. Amplification of D1S80 locus can be acquired from adding the boving serum albumin and hot start PCR procedures from forensic samples such as saliva stains (cigarette butts), so the AMP-FLPs examining is possible. 4. Genotype could be determined simply and rapidly using Amplitype$TM$ HLA-DQ$\alpha$ forensic kit in examining the HLA-DQA1 gene. From the investigation, DNA extraction, sex determination, amplification of D1S80 locus, polymorphisms of HLA-DQA1 gene was successfully done even though the cigarette butts were left for 15 days at room temperature. Therefore cigarette butts are highly reliable and applicatable as molecular biologic samples for individual identification.

• 아동학회지
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• 제20권3호
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• pp.259-273
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• 1999

This studied investigated children's music identification, classification, and seriation cognitive task performance abilities by age and sex. The subjects were l20 six-, eight-, and ten-year-old school children. There were significant positive correlations among music cognition tasks and significant age and sex differences within each of the music tasks. Ten-year-old children were more likely to complete their music identification tasks than the younger children and girls were more likely than boys to complete their music identification tasks. Eight- and 10-year-old children were more likely to complete their music classification tasks than the younger group. Piagetian stage theory was demonstrated in children's music classification task performance. There was an age-related increase in the performance of the music seriation tasks. Developmental sequential theory was demonstrated in music seriation performance.

• Molecules and Cells
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• 제26권3호
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• pp.314-318
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• 2008

Korean long-tailed goral (Nemorhaedus caudatus) is one of the most endangered species in South Korea. However, detailed species distribution and sex ratio data on the elusive goral are still lacking due to difficulty of identification of the species and sex in the field. The primary aim of this study was to develop an economical PCR-RFLP method to identify species using invasive or non-invasive samples from five Korean ungulates: goral (N. caudatus), roe deer (Capreolus pygargus), feral goat (Capra hircus), water deer (Hydropotes inermis) and musk deer (Moschus moschiferus). The secondary aim was to find more efficient molecular sexing techniques that may be applied to invasive or non-invasive samples of ungulate species. We successfully utilized PCR-RFLP of partial mitochondrial cytochrome b gene (376 bp) for species identification, and sex-specific amplification of ZFX/Y and AMELX/Y genes for sexing. Three species (goral, goat and water deer) showed distinctive band patterns by using three restriction enzymes (Xbal, Stul or Sspl). Three different sexing primer sets (LGL331/335 for ZFX/Y gene; SE47/48 or SE47/53 for AMELX/Y gene) produced sex-specific band patterns in goral, goat and roe deer. Our results suggest that the molecular analyses of non-invasive samples might provide us with potential tools for the further genetic and ecological study of Korean goral and related species.

• 대한수의학회지
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• 제45권1호
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• pp.127-130
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• 2005

The objective of present study was to ascertain sex determination for individual identification, parentage control, and sex chromosome anomalies in horse. PCR amplification products of the equine sex determining region of the Y chromosome gene (SRY) and amelogenin gene (AMEL) were detected by using agarose gel electrophoresis. A normal sire and foal II showed 1 SRY band (430 bp) and 3 AMEL (AMELX, AMELY, and AMELX/Y) band, 175 bp, 160 bp, 190 bp, respectively, and a normal dam and foal I showed a single AMELX band (175 bp). These results enables a quick diagnosis for sex determination prior to cytogenetic analysis.