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Identification of Species and Sex of Korean Roe Deer (Capreolus pygargus tianschanicus) Using SRY and CYTB Genes  

Han, Sang-Hyun (National Institute of Subtropical Agriculture, Rural Development Adminstration)
Cho, In-Cheol (National Institute of Subtropical Agriculture, Rural Development Adminstration)
Lee, Sung-Soo (National Institute of Subtropical Agriculture, Rural Development Adminstration)
Tandang, Leoncia (National Institute of Subtropical Agriculture, Rural Development Adminstration)
Lee, Hang (Conservation Genome Resource Bank for Korean Wildlife (CGRB), College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
Oh, Hong-Shik (Department of Science Education, Cheju National University)
Kim, Byoung-Soo (Department of Life Science, Cheju National University)
Oh, Moon-You (Department of Life Science, Cheju National University)
Publication Information
Animal cells and systems / v.11, no.2, 2007 , pp. 165-168 More about this Journal
Abstract
The nucleotide sequences of a male-specific marker sex determining region Y (SRY) gene and a mitochondrial cytochrome B (CYTB) gene were characterized and analyzed to establish a molecular method for identification of species and sex of Korean roe deer (Capreolus pygargus tianschanicus). Similarity search result of SRY sequences showed very similar result to those reported in Moose (Alces alces) and Reindeer (Rangifer tarandus), both of which had 95.9% similarity in identity. CYTB genes were very similar to those reported in Siberian roe deer (C. pygargus pygargus) which had 98.6% similarity and not to European roe deer (C. capreolus), suggesting that the DNA samples tested were of Siberian roe deer lineage. Polymerase chain reaction (PCR)-based sex typing successfully discriminated between carcasses of male and female roe deer. Males had SRY band on agarose gels and females did not. The result of this molecular sex typing provided similar information with that obtained by genital organ observation. Therefore, this molecular method using male specific marker SRY and mitochondrial CYTB genes would be very useful for identification of the species and sex of the carcass remains of roe deer.
Keywords
species identification; sex determination; roe deer; SRY; CYTB;
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