• ALGAE
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• v.39 no.2
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• pp.109-127
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• 2024

High production and efficient harvesting of microalgae containing high omega-3 levels are critical concerns for industrial use. Aeration can elevate production of some microalgae by providing CO₂ and O₂. However, it may lower the production of others by generating shear stress, causing severe cell damage. The mixotrophic dinoflagellate Gymnodinium smaydae is a new, promising microalga for omega-3 fatty acid production owing to its high docosahexaenoic acid content, and determining optimal conditions and methods for high omega-3 fatty acid production and efficient harvest using G. smaydae is crucial for its commercial utilization. Therefore, to determine whether continuous aeration is required, we measured densities of G. smaydae and the dinoflagellate prey Heterocapsa rotundata in a 100-L semi-continuous cultivation system under no aeration and continuous aeration conditions daily for 9 days. Furthermore, to determine the optimal conditions for harvesting through centrifugation, different rotational speeds of the continuous centrifuge and different flow rates of the pump injecting G. smaydae + H. rotundata cells into the centrifuge were tested. Under continuous aeration, G. smaydae production gradually decreased; however, without aeration, the production remained stable. Harvesting efficiency and the dry weights of omega-3 fatty acids of G. smaydae + H. rotundata cells at a rotational speed of 16,000 rpm were significantly higher than those at 2,000-8,000 rpm. However, these parameters did not significantly differ at injection pump flow rates of 1.0-4.0 L min^-1. The results of the present study provide a basis for optimized production and harvest conditions for G. smaydae and other microalgae.

• Steel and Composite Structures
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• v.48 no.3
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• pp.251-262
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• 2023

There are many examples of steel structures subjected to severe environmental conditions with bolted connections directly exposed to extreme climatic agents such as freeze-thaw cycles or low temperatures. Some examples are: steel bridges, mining transfer towers, wind towers... These service conditions neither are included in Eurocode 3 or EN1090-2, nor there are references in other international standards. In this experimental research, 46 specimens of non-slip joints with HV M20 bolts and four different types of contact surfaces have been studied. Half of the specimens were subjected to fourteen twelve-hours freeze-thaw cycles, with periodic immersion in water and temperature oscillation. Subsequently, half of the connections were subjected to a slip test under monotonic load at temperature of -20 ± 0.5 ℃ and the other half at room temperature. The results were compared with others equal joints not subjected to freeze-thaw cycles and kept at room temperature for the same time. This finally resulted in 4 sets of joints by combining the freeze-thaw degradation or not with the low-temperature conditions or not in the slip testing. Therefore, a total of 16 different conditions were studied by also considering 4 different contact surfaces between the joined plates in each set. The results obtained show influence of environmental conditions on the slip resistant capacity of these joints.

• Computers and Concrete
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• v.5 no.6
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• pp.573-597
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• 2008

Among artificial intelligence-based computational techniques, adaptive neuro-fuzzy inference systems (ANFIS) are particularly suitable for modelling complex systems with known input-output data sets. Such systems can be efficient in modelling non-linear, complex and ambiguous behaviour of cement-based materials undergoing single, dual or multiple damage factors of different forms (chemical, physical and structural). Due to the well-known complexity of sulfate attack on cement-based materials, the current work investigates the use of ANFIS to model the behaviour of a wide range of self-consolidating concrete (SCC) mixture designs under various high-concentration sodium sulfate exposure regimes including full immersion, wetting-drying, partial immersion, freezing-thawing, and cyclic cold-hot conditions with or without sustained flexural loading. Three ANFIS models have been developed to predict the expansion, reduction in elastic dynamic modulus, and starting time of failure of the tested SCC specimens under the various high-concentration sodium sulfate exposure regimes. A fuzzy inference system was also developed to predict the level of aggression of environmental conditions associated with very severe sodium sulfate attack based on temperature, relative humidity and degree of wetting-drying. The results show that predictions of the ANFIS and fuzzy inference systems were rational and accurate, with errors not exceeding 5%. Sensitivity analyses showed that the trends of results given by the models had good agreement with actual experimental results and with thermal, mineralogical and micro-analytical studies.

• Wind and Structures
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• v.20 no.5
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• pp.683-699
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• 2015

Lighting poles and antenna masts are typically high, slender and light structures. Moreover, they are often characterized by distributed eccentricities that make very complex their shape. Experience teaches that this structural type frequently suffers severe damage and even collapses due to wind actions. To understand and interpret the aerodynamic and aeroelastic behavior of lighting poles and antenna masts, this paper presents the results of static and aeroelastic wind tunnel tests carried out on a complex prismatic element representing a segment of the shaft of such structures. Static tests are aimed at determining the aerodynamic coefficients and the Strouhal number of the test element cross-section; the former are used to evaluate the critical conditions for galloping occurrence based on quasi-steady theory; the latter provides the critical conditions for vortex-induced vibrations. Aeroelastic tests are aimed at reproducing the real behavior of the test element and at verifying the validity and reliability of quasi-steady theory. The galloping hysteresis phenomenon is identified through aeroelastic experiments conducted on increasing and decreasing the mean wind velocity.

• Explosives and Blasting
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• v.12 no.3
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• pp.32-41
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• 1994

The specific characters in recent Chemical processing and industrial plants are the handling of severe limit conditions such as high pressure, high and low temperature, high speed production and transportation plant automation and appearance of many new hazardous materials in numbers and quantity. The possibilities of these characters causing the accident and environmental pollution in industrial activities become very high. This paper describes the concrete policy In prevent the explosion accident out of the many accidents which are the safety of the safety engineering.

• Proceedings of the Korean Society For Composite Materials Conference
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• 2002.05a
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• pp.151-154
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• 2002

The durability of two kinds of CFRP plates, carbon/UP and carbon/V, was studied under severe environmental conditions. Immersion into the chemical solutions and accelerated weathering were executed on the CFRP plates and the weight change under each condition was measured. After those treatments, the plates were tested by tensile testing machine to measure the mechanical properties and observed by SEM to find the damage behaviour of the surface. Comparing the virgin plates and the chemically exposed plates, both plates show severe deterioration of the mechanical properties. But, the plates subjected to alkali solution show much larger reduction than those of acidic solution and carbon/V has better chemical resistance than carbon/UP. The material properties of CFRP plate exposed to the weathering were deteriorated linearly proportional to the exposure time.

• Journal of Ocean Engineering and Technology
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• v.14 no.3
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• pp.1-10
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• 2000

In order to effective manage the construction, disaster prevention plan and the harbor tranquility control, meteorological and sea-state characteristics around Cheju Island have been analyzed. Using results and damage examples of the port facilities under severe sea conditions, a reasonable construction control plan considering the regional characteristics of the sea-state and winds was proposed. That is, in northern part of Cheju Island, the construction work is affected mainly by the winter storms, while the typhoon mainly affects the southern part port facilities during summer to Autumn. Considering their typical characteristics, it is strongly suggested that the main construction work should be carried out during April to July in the northern part, and it should be made during October to next July in the southern part of the island. A permeable TTP mounded breakwater was constructed to protect severe waves as a temporal structure under the long-term development plan in Sogipo port. The transmission characteristics of the structure was discussed using the experimental results. The results show that the transmission coefficient $K_{t}$ is over 20% of incident waves, which cause many problems in the cargo handling in relation to harbor tranquility. In conclusion, this kind of permeable structure can be used only as a temporal structure for the disaster prevention under the construction process. It causes many problems in harbor tranquility if it is used as a permanent harbor structures.s.

• The Plant Pathology Journal
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• v.20 no.2
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• pp.92-96
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• 2004

Environmental factors such as soil moisture, land management, and weather conditions affecting Fusarium wilt of sweet potato were investigated in major sweet potato cultivation regions in Korea. Fusarium wilt occurred mainly in reclaimed terracing lands, which are flattened and located in hilly to mountainous areas at the base of the mountain, in early seasonal cultivation regions. Disease severity was lower in reclaimed fields with natural slope. The development of Fusarium wilt in the fields was highly correlated with precipitation during planting period (r=-0.96**). Fusarium wilt was more severe in fields with less than 20 cm of available soil depth than in fields with over 20 cm of available soil depth. Greenhouse studies were consistent with field studies that less soil moisture content caused severe Fusarium wilt of sweet potato. These results indicate that low rainfall and moisture of soil with low effective soil depth during planting period are important environmental factors influencing the development of Fusarium wilt.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.4 no.1
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• pp.59-69
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• 2000

The present study was undertaken in investigate the response to salinity and effect of plant growth regulators and proline under salinity stress on the germination and seedling growth of Vigna angularis. The protective effect of external Ca2+ on root elongation under saline conditions was also investigated. The seed germination of Vigna angularis decreased with an increase in salinity. The growth regulators GA3 was more effective than kinetin. At a higher salinity, low concentrations of kinetin and high concentrations of GA3 were more effective. The external application of proline and betaine improved germination under saline conditions. At a low salinity proline and betaine alleviated the salinity-induced inhibition of germination, yet at higher NaCl concentrations, proline and betaine were both ineffective. Exposure to salinity during germination was accompanied by an increase in the proline content, thereby suggesting that one compatible solute in the germinating seed would seem to be proline. The inhibition of germination by high NaCl concentrations was relatively more severe in scarified seeds than in intact seeds, indicating that the seed coat acts as a partial barrier to an Na2+ ameliorated the adverse effect of salinity stress.

• KSBB Journal
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• v.13 no.6
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• pp.662-668
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• 1998

Viruses belonging to the Hantavirus genus cause two acute severe illness in humans, i.e., Haemorrhagic Fever with Renal Syndrome (HFRS) and Hantavirus Pulmonary Syndrome(HPS). Among them, Hantaan virus is one of the most important viruses causing HFRS. Recombinant expression vectors, pKK-NP and pET-NP, with Hantaan viral nucleocapsid gene were constructed, and used to transform Eschericia coli BL21(DE3). Stability of the vectors in the host strain, and effects of some environmental conditions on the expression of nucleocapsid gene were studied. Expression vector, pKK-NP, was very unstable, and the expression level of nucleocapsid gene was very low compared to that of pET-NP. BL21(pET-NP) produced about 100 mg of N protein per liter of culture broth. Induction time did not show any significant difference on the expression level of nucleocapsid gen and cell growth. BL21(pET-NP) culture at 35$^{\circ}C$ showed a little higher expression level than at 30$^{\circ}C$ during growth phase, but reached to the same level at stationary phase. Total expression level was proportional to supplemented glucose concentration of media up to 0.5% along with cell growth, but expression level per unit cell mass was inversely proportional to glucose concentration and maximal when glucose was not supplemented at all.