• 제목/요약/키워드: Settled cells volume

검색결과 5건 처리시간 0.019초

닭 및 산양의 이종plasma내에서 적혈구 침강에 대한 연구 (Studies on the red blood cell sedimention rates in heteroplasma of chicken and goat)

  • 유창준;이수두
    • 대한수의학회지
    • /
    • 제28권2호
    • /
    • pp.271-277
    • /
    • 1988
  • In order to study the marked variation of red blood cell sedimentation rate in some species of animals, the packed cell volume, volume percentage of erythrocytes in whole blood, was reshuffled of 20%, 40% and 60% using heteroplasma of chicken and goat, and the red blood cell sedimentation rate was measured in Westergren tubes at $27{\pm}1^{\circ}C$ and $8{\pm}1^{\circ}C$. The results obtained were summarized as follows: 1. The values of packed cell volume(PCV) of goat and chicken were $40.7{\pm}4.1%$ and $30.2{\pm}2.2%$ respectively. 2. The sedimentation rates of reshuffled red blood cell were settled faster at lower PCV than higher PCV, i.${\acute{e}}$. there was a reverse relationship between the sedimention rate and PCV. 3. Red blood cells of chicken settled quickly, where as those of goat settled very slowly. Chicken red blood cell sedimented rapidly even in goat plasma, and goat red blood cell sedimented slowly in chicken plasma. These findings indicate that the plasma is not the only factor determining the rapid red blood cell sedimentation rate of chicken. 4. The sedimentation rate of reshuffled red blood cell of chicken and goat were accelerated at higher temperature than lower temperature.

  • PDF

낙엽송(Larix kaempferi) 현탁배양된 배발생세포로부터 체세포배 유도 및 발아를 위한 삼투압제 종류 및 농도 효과 (Effect of kinds and concentrations of osmoticum on somatic embryo induction and germination from suspended embryogenic cell in Larix kaempferi)

  • 김용욱
    • Journal of Plant Biotechnology
    • /
    • 제40권3호
    • /
    • pp.141-146
    • /
    • 2013
  • 본 연구는 낙엽송 배발생세포의 배양기간에 따른 현탁세포의 생장, 체세포배 유도 및 발아를 위한 다양한 삼투압제 종류 및 농도 효과를 조사하기 위해 수행되었다. 배발생 현탁세포의 생장은 배양일수가 증가함에 따라 settled cells volume (SCV)가 지속적으로 증가하는 경향을 보여 배양 18일 경에는 최대 10.1 ml의 SCV를 보였고, 그 이후로는 세포생장이 감소하였다. 현탁세포로부터 체세포배 발생을 위한 삼투압제 종류 및 농도에 따른 효과 비교에서 최대의 체세포배 유도 수는 0.2 M sucrose 처리구에서 352.3개(/g FW)로 나타났으며, 0.2 M maltose (203.7) 및 0.3 M maltose (193.7) 처리구에서 또한 효과가 있었다. 그러나 7.5% polyethylene glycol (PEG)이 첨가된 0.15 M sucrose 혹은 maltose 각각의 처리구에서는 체세포배 발생이 전혀 이루어지지 않았다. 다양한 삼투압제 종류 및 농도에서 발생된 체세포배의 발아율 비교에서는 최대 자엽 형성율(25.2%) 0.3 M maltose, 하배축 형성률(39%), 뿌리 발생율(30.3%) 및 식물체 재분화율(3.5%)은 0.2 M sucrose 처리구에서 각각 나타났다.

고르지 않은 바닥을 지나는 천수 흐름에 대한 유한체적 모형 (Finite-Volume Model for Shallow-Water Flow over Uneven Bottom)

  • 황승용
    • 한국수자원학회논문집
    • /
    • 제46권2호
    • /
    • pp.139-153
    • /
    • 2013
  • 고르지 않은 바닥을 지나는 천수 흐름을 해석하기 위해 천수방정식의 흐름률 경사항과 바닥 경사 생성항에 대해 HLLL 기법과 DFB(Divergence Form for Bed slope source term) 기법을 각각 적용하여 유한체적 모형을 구성하였다. 또한, PSC(Partially Submerged Cell)의 고려를 위해 VFR(Volume/Free-surface Relationship)도 이용하였다. MUSCL에서 WSDGM(Weighted Surface-Depth Gradient Method)을 보다 단순하게 고쳐도 원래의 방법과 정확도가 동등함을 1차원 정상 흐름에 대해 확인하였다. 1차원 PSC에 대한 VFR를 통해 흐름률 경사항과 바닥 경사 생성항의 선평형성이 정확하게 충족됨을 입증하였다. 2차원 PSC에서 DFB 기법으로는 지배방정식의 선평형성이 충족되지 않은 문제를 삼각형 격자에 대한 VFR를 이용하여 해소하였다. 삼각형 턱과 둥근 융기를 지나는 2차원 댐 붕괴 흐름에 대한 모의에서 실험실 실험 결과와 잘 부합됨을 확인하였다. 또한, 부분 댐 붕괴 흐름에 대한 모형의 적용에서 경사면은 물론 불규칙 바닥에서도 요철의 잠김이 성공적으로 모의되었다. 따라서 고르지 않은 실제 하천 지형에 대한 이 모형의 적용성이 기대된다.

박테리아의 산소소비량에 관한 실험적 고찰 (Respiratory Activity of Bacteria in Various Concentrations of Glucose)

  • 최명자
    • Journal of Preventive Medicine and Public Health
    • /
    • 제10권1호
    • /
    • pp.134-137
    • /
    • 1977
  • The most efficient method for reducing the organic content of dilute liquid waste is by aerobic-biological treatment. Basically, the organisms responsible for treatment possess the ability to decompose complex organic compounds and to use the energy so liberated for their bodily functions: reproduction, growth, locomotion and so on. That part of organic matter used to produce energy is converted to the essentially stable end products of carbon dioxide, water and ammonia, while the remainder is converted to new cells which can be settled and thus removed from the liquid before the waste is discharged to the receiving body water. Oxygen must be supplied continuously during the aerobic process. In the field of sewage treatment the Warburg respirometer is used mainly for the measurement of the oxygen uptake of samples. In this experiment the Warburg constant volume respirometer was used to determine the oxygen uptake by bacteria in the presence of various glucose concentrations. The rate of oxygen uptake by the bacteria was expressed as the respiratory quotient. The result indicated that the oxygen uptake was proportional to the glucose concentration. The expecting equation of the regression line was Y=7.7+0.12X where Y: respiratory quotient, ${\mu}l.\;O_2$ taken up/mg. dry wt. bacterium/hr. X: concentration of glucose, mg/l

  • PDF

낙동강 수계의 식물플랑크톤 침강속도 (Settling Velocity of Phytoplankton in the Nakdong-River)

  • 정유경;김범철;신명선;박주현
    • 한국물환경학회지
    • /
    • 제23권6호
    • /
    • pp.807-813
    • /
    • 2007
  • Settling velocity is one of major parameters determining algal biomass in water quality modeling. In this study, the settling velocity of phytoplankton was measured in reservoir and stream sites of the Nakdong River, Korea. Settling velocities of various phytoplankton species were determined by measuring algal cell biomass settled in a sedimentation cylinder. Mean settling velocities were $0.22m\;day^{-1}$ in reservoir sites and $0.33m\;day^{-1}$ in stream sites, which were relatively higher compared with other default values suggested by water quality models (e.g. $0.1m\;day^{-1}$ in CE-QUAL-W2). The lower settling velocity in reservoirs than in stream implies the adaptation of phytoplakton to low turbulence in lentic environments. Cyanobacteria showed lower settling velocity ($0.2m\;day^{-1}$) than diatoms ($0.3m\;day^{-1}$), and this phenomenon may have resulted from buoyancy mechanisms of cyanobacteria. Cell volume did not show a significant correlation with settling velocity in this study, implying that conformation factors of colonies or other factors had large effects on settling velocity of algal cells as well as cell size. The result of this study may suggest proper coefficients of settling velocity of phytoplankton in the calibration of water quality model.