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http://dx.doi.org/10.5010/JPB.2013.40.3.141

Effect of kinds and concentrations of osmoticum on somatic embryo induction and germination from suspended embryogenic cell in Larix kaempferi  

Kim, Yong-Wook (Div. of Forest Biotechnology, Korea Forest Research Institute)
Publication Information
Journal of Plant Biotechnology / v.40, no.3, 2013 , pp. 141-146 More about this Journal
Abstract
This study was conducted to examine suspended embryogenic cells growth with days of culture, effects of various kinds/concentrations of osmoticum for induction of somatic embryos (SEs), following somatic embryos germination or plantlet regeneration. The proliferation pattern of embryogenic cells in suspension culture is characterized by settled cells volume (SCV) increased with the duration of culture with marked the maxium of SCV (10.1 ml) in 18 days of culture, however the SCV of cells gradually decreased after that. In comparison of kinds/concentrations of osmoticum on somatic embryo induction, the highest induction number (352.3/g FW) of the SE was showed in 0.2 M sucrose, in addition, we also observed some effects with treatments of 0.2 M maltose (203.7) and 0.3 M maltose (193.7), respectively. However, no somatic embryos produced in treatments of 7.5% PEG plus 0.15 M sucrose or maltose. In comparison of germination efficiency of SEs which occurred from the treatments of various kinds/ concentrations of osmoticum, the highest induction frequency of cotyledon (25.2%) was obtained from SEs that produced 0.3 M maltose, however, the best occurrence rates of hypocotyl (39%), radicle (30.3%) and plantlet regeneration (3.5%) were observed from the 0.2 M sucrose treatment, respectively.
Keywords
Germination; Japanese larch; Larix kaempferi; Settled cells volume; Somatic embryo; Osmoticum; Suspended cells;
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1 Ali MB, Thanh NT, Yu KW, Hahn EJ, Paek KY, Lee HL (2005) Induction in the antioxidative systems and lipid peroxidation in suspension culture toots of Panax ginseng induced by oxygen in bioreactors. Plant Sci 169:833-841   DOI   ScienceOn
2 Dong JZ, Dunstan D (1994) Growth parameters, protein and DNA synthesis of an embryogenic suspension culture of white spruce (Picea glauca). J Plant Physiol 144:201-208   DOI
3 Finer J, Kriebel HB, Becwar MR (1989) Initiation of embryogenic callus and suspension cultures of eastern white pine (Pinus strobus L.). Plant Cell Rep 8:203-206   DOI   ScienceOn
4 Gupta PK, Timmis R (2005) Mass propagation of conifer trees in liquid cultures-progress towards commercialization. Plant Cell Tiss Org Cult 81:339-346   DOI
5 Hakman I, von Arnold S (2000) Somatic embryogenesis and plant regeneration from suspension cultures of Picea glauca (White spruce). Physiol Plant 72:579-587
6 Ingram B, Mavituna F (2000) Effect of bioreactor configuration on the growth and maturation of Picea sitchensis somatic embryo cultures. Plant Cell Tiss Org Cult 61:87-96   DOI
7 Kim YW (2010) Somatic embryogenesis and plant regeneration with embryogenic tissue lines in Larix leptolep. J Kor For Soc 99:633-637
8 Kim YW, Moon HK (2007) Enhancement of somatic embryogenesis and plant regeneration in Japanese larch (Larix leptolepis). Plant Cell Tiss Org Cult 88:241-245   DOI   ScienceOn
9 Kim YW, Moon HK (2009) Effect of TIBA, PCIB and phloroglucinol on somatic embryo maturation and germination in Japanese larch (Larix leptolepis). J Plant Biotech 36:230-235   과학기술학회마을   DOI   ScienceOn
10 Kim YW, Youn Y, Noh ER, Kim JC (1999) Somatic embryogenesis and plant regeneration from immature zygotic embryos of Japanese larch (Larix leptolepis). Plant Cell Tiss Org Cult 55:95-101
11 Klimaszewska K, Devantier Y, Lachance D, Lelu MA, Charest PJ (1997) Larix larcina (tamarack): somatic embryogenesis and genetic transformation. Can J For Res 27:538-550
12 Krogstrup P. (1990) ffect of culture densities on cell proliferation and regeneration from embryogenic cell suspensions of Picea sitchensis. Plant Sci 72:115-123   DOI   ScienceOn
13 Lelu MA, abel P (1994) Changes in the levels of abscisic acid and its glucose ester conjugate during maturation of hybrid larch (Larix${\times}$leptoeuropaea) somatic embryos, in relation to germination and plantlet recovery. Physiol Plant 92:53-60   DOI   ScienceOn
14 Lelu MA, Paques L (2009) Simplified and improved somatic embryogenesis of hybrid larches (Larix${\times}$eurolepis and Larix${\times}$marschlinsii). Perspectives for breeding. Ann For Sci 104:1-10
15 Litvay JD, Verma DC, Johnson MA (1985) Influence of a loblolly pine (Pinus taeda L.) culture medium and its components on growth and somatic embryogenesis of the wild carrot (Darcus carota L.). Plant Cell Rep 4:325-328   DOI   ScienceOn
16 Paek KY, Chakrabarty D, Hahn EJ (2005) Application of bioreactor systems for large scale production of horticultural and medicinal plants. Plant Cell Tiss Org Cult 81:287-300   DOI   ScienceOn
17 Lulsdorf MM, Tautorus TE, Kikcio SI, Dunstan DI (1992) Growth parameters of embryogenic suspension cultures of interior spruce (Picea glauca-engelmannii complex) and black spruce (Picea mariana Mill). Plant Sci 82:227-234   DOI   ScienceOn
18 Nadel BL, Altman A, Ziv M (1989) Regulation of somatic embryogenesis in celery cell suspensions. Plant Cell Tiss Org Cult 18:181-189   DOI
19 Nagmani R, Bonga JM (1985) Embryogenesis in subcultured callus of Larix decidua. Can J For Res 15:1088-1091   DOI
20 Ramakrishnan K, Gnanam R, Sivakumar P (2005) In vitro somatic embryogenesis from cell suspension cultures of cowpea {Vigna unguiculata (L.) Walp}. Plant Cell Rep 24:449-461   DOI
21 Scott P, Lyne RL, Rees TA (1995) Metabolism of maltose and sucrose by microspores isolated from barley (Hordeum vulgare). Planta 197:435-441
22 Shoji M, Sato H, Nakagawa R, Funada R, Kubo T, Ogita S (2006) Influence of osmotic pressure on somatic embryo maturation in Pinus densiflora. J For Res 11:449-453   DOI   ScienceOn
23 Tautorus TE, Lulsdorf MM, Kikcio SI, Dunstan DI (1992) Bioreactor culture of Picea mariana Mill. (black spruce) and the species complex Picea glauca-engelmannii (interior spruce) somatic embryos. Growth parameters. Appl Microbiol Biotechnol 38:46-51
24 Yildirim T, Kaya Z, Isik K (2006) Induction of embryogenic tissue and maturation of somatic embryos in Pinus brutia TEN. Plant Cell Tiss Org Cult 87:67-76   DOI   ScienceOn